ASHS 2024 Conference

In this interactive workshop, participants will be provided training on how to use Rosaceae (GDR, www.rosaceae.org), Vaccinium (GDV, www.vaccinoum.org) and Citrus (www.citrusgenomedb.org) specialty crop databases resources, as well as the Breeding Data Management tools, BIMS (www.breedwithbims.org), and the Field Book App for phenotype data collection. Using a case-study approach we will focus on how to use these integrated resources most efficiently for research and crop improvement efforts, how to apply FAIR data principles to sharing and submitting research data to these databases at the time of publication and facilitate a robust dialogue between researcher, breeders and the development team on needed improvements and long-term sustainability options for these resources.

This interactive workshop will bring together researchers and breeders to accelerate research and crop improvement in specialty crops. It will provide training and feedback on how to most effectively use the NIFA SCRI and National Research Support project 10 (NRSP10) funded Rosaceae, Citrus, and Vaccinium specialty crop databases and breeding data management resources (BIMS and Field Book), learn how to follow FAIR data principles, foster better two-way communication for increased feedback from researchers on further development of these resources, and continue to build a robust community around these research and crop improvement tools. The trainers include users and developers of these resources, ranging from early career to well-known and respected research enabling resource generators.

The Genome Database for Rosaceae (GDR), Genome Database for Vaccinium, and Citrus Genome Database (CGD) offer critical resources and tools to enable genomic, genetic, and breeding research for fruit, nut, and ornamental crops of great economic importance to the U.S. In addition, BIMS (BreedwithBIMS.org) and the Field Book APP provide widely used tools for managing plant breeding program data. While these resources are heavily used worldwide, many researchers are unaware of the full potential of using them and how they can contribute their own data for wider recognition and reuse. This interactive workshop aims to bridge this knowledge gap by providing hands-on training for specialty crop researchers on how to best use these resources and contribute their data. The workshop will also solicit ideas from participants on how to improve these databases and discuss the various options to make them sustainable in the long term. The workshop will bolster the utilization of integrated big data, promote future data sharing, and ensure that data is FAIR (Findable, Accessible, Interoperable, and Reusable).

The workshop aims to bring together researchers to accelerate research by more effective use of specialty crop databases and breeding data management resources, encouraging data submission at the time of publication, and gaining feedback from researchers. Through hands-on training, participants will become more familiar with the database resources and breeding data management tools, learn how to submit their data, and provide essential input for improving these databases and their long-term sustainability.

Coordinator(s)

• Dorrie Main, Washington State University, Pullman, WA, United States

Moderator(s)

• Cameron Peace, Washington State University, Horticulture, Pullman, WA, United States

Speaker/Participant(s)

• Dorrie Main, Washington State University, Pullman, WA, United States

Introduction to the Workshop and Specialty Crop Research Databases (15 mins)

• Jodi Humann, Washington State University, Horticulture, Pullman, WA, United States

How to efficiently use integrated genomics data and tools for research (20 mins)

• Sook Jung, Washington State University, Horticulture, Pullman, WA, United States
  How to efficiently use integrated genetics data and tools for research (20 mins)
  
  
• Nahla Bassil, USDA ARS, National Clonal Germplasm Repository, Corvallis, OR, United States
  How to efficiently use integrated germplasm and genotype data for research (15 mins)
  Summary:
  
  
• Trevor Rife, Clemson University, Plant and Environmental Sciences Department, Florence, SC, United States
  How to use the Field Book App for phenotypic data collection (15 mins)
• Sushan Ru, Auburn University, Auburn, United States
  How to use the Breeding Information Management System, BIMS, for Crop Improvement (20 mins)
• Cameron Peace, Washington State University, Horticulture, Pullman, WA, United States

Effect of Gamma Irradiation on Survival, Fruit Characteristic, and Thorn Length in Different Varieties of Citrus - Dong-Hoon Lee
Consumer Preference and Willingness-to-Pay for Pawpaw Value-Added Products - Sheri Crabtree
Promising Advanced Selections from Kentucky State University’s Pawpaw (Asimina triloba) Breeding Program. - Jeremy Lowe
Assessment of Fruit Quality and Value-added Potential of 16 Pawpaw Cultivars and Advanced Selections - Kirk Pomper
Effects of Temperature and Light on Sunburn, and Preventing the Sunburn Damage by Chemical Suppressants in ‘Fuyu’ Sweet Persimmons - Yunji Shin
Crown Gall Associated with Higher Risk of New Butt Rot Pathogen in California Almond Orchards - Raymond Mireles
Assessment of Biomass and Carbon Storage of Satsuma Mandarin Trees in Jeju Island, Korea - Jee-Soo Park

In this study, we investigated the variety-dependent survival of citrus seedlings and analyzed the pomological characteristics of fruits and thorn lengths following irradiation with gamma rays. Scions from the seedlings of the following citrus varieties, ‘Yuzu’, ‘Noeulhyang’, ‘Sarahyang’, ‘Namgam’ (nucellar), and ‘Meiwakumquat’ irradiated with gamma rays at 0, 60, 80, and 100 Gy were grafted onto trifoliate orange. At 6 months after grafting, the survival rates of ‘Yuzu’ and ‘Noeulhyang’ exposed to 100 Gy were 49.5% and 34.8%, and that of ‘Sarahyang’ and ‘Namgam’ (nucellar) exposed to 80 Gy were 51.6% and 46.8%, respectively. ‘Meiwakumquat’ exhibited the lowest survival among the varieties used in this study. Therefore, we classified ‘Meiwakumquat’ as highly susceptible to gamma radiation; ‘Noeulhyang’, ‘Sarahyang’, and ‘Namgam’ (nucellar) as intermediately susceptible; and ‘Yuzu’ as the least susceptible. We assessed the effects of gamma radiation on the pomological traits in irradiated ‘Tambit1ho’ and ‘Yuzu’. Among the 72 irradiated ‘Tambit1ho’ seedlings, the average seed number (number of seeds per fruit) varied between 0 and 18.6, whereas that in the unirradiated seedlings was between 8 and 18. Among the irradiated seedlings, the average seed number was less than 1.0 in eight seedlings. In addition, we observed variations in weight, rind and segment hardness, and sugar and acid contents. The thorn length of the flush in unirradiated ‘Yuzu’ was between 1.3 and 6.0 cm, whereas that of flush in gamma-irradiated seedlings was between 0.1 and 6.1 cm. Among the irradiated ‘Yuzu’ seedlings, the thorn length was less than 0.1 cm in two seedlings.(This work was carried out with the support of the Research Program for the National Institute of Horticultural

The North American pawpaw (Asimina triloba) is the largest edible tree fruit native to North America, with a flavor resembling a unique tropical-like blend of mango and banana. Commercial production and consumer interest in pawpaw is on the rise in the US and worldwide. Fresh pawpaw fruit has a short shelf life and does not store or ship well. Value-added products represent a great potential market to both diversify offerings of products containing pawpaw, and provide shelf-stable products that could be available year-round and circumvent the storage and shipping challenges of fresh fruit. Pawpaws can be utilized in many value-added products, including ice cream, yogurt, jam, custard, baked goods, hot sauces, wine, beer, and brandy. A pawpaw value-added product tasting was conducted in September 2023 at the Kentucky State University Research and Demonstration Farm as part of the annual Pawpaw Third Thursday Thing workshop. Participants tasted samples of slices of fresh pawpaw fruit of six varieties, pawpaw jam on crackers, and pawpaw ice cream. A survey was conducted evaluating preference and interest in purchasing pawpaw fruit, jam, and ice cream, and willingness to pay. The survey found that 93% of participants would be interested in purchasing pawpaw fruit at a farmers market, compared to 80% at a grocery store and 88% at an on-farm market or roadside stand. Ninety-five percent (95%) of participants would be interested in purchasing pawpaw jam if available in stores, and 95% of participants would also be interested in purchasing pawpaw ice cream if available in stores. Respondents would be willing to pay $2.20 per fruit, or $4.97 per pound for fresh pawpaw fruit. Respondents reported they would pay $7.22 for an 8 ounce jar of pawpaw jam, and $8.51 for a quart of pawpaw ice cream. Currently, pawpaw ice cream and jam are sold by very few retailers in specialty markets, so there is great opportunity for pawpaw producers to introduce high-value pawpaw products that are desired by consumers to their retail markets.

The North American Pawpaw (Asimina triloba) is a tree fruit native to the Eastern U.S. which is being produced in commercial orchards across the U.S. and internationally, with an increasing market and interest. There are approximately 50 pawpaw cultivars available, but many of these varieties have low yields with fruit sizes below the threshold of 120 grams needed for commercial sale and processing. New high yielding cultivars with excellent fruit quality would further assist in the development of the pawpaw industry. Kentucky State University serves as the National Clonal Germplasm Repository for Pawpaw. Two goals of the Repository research efforts are germplasm acquisition and evaluation. The repository contains over 2000 accessions from 16 different states; additionally, both open pollinated seedlings from superior genotypes and crosses of superior selections have been incorporated into the repository collection. Fruit weights from three of KSU’s advanced selections (Hi 1-4, Hi 7-1, and G6-120) were compared to the cultivars ‘KSU-Atwood’, ‘KSU-Benson’, ‘KSU-Chappell’, ‘Ralph’s Whopper’, and ‘Sunflower’. Fruit weights for Hi 1-4 (241.5 g) and Hi 7-1 (233.5 g) were significantly larger than ‘Sunflower’ (145.3 g). Hi 1-4 and Hi 7-1 show potential to be promising new releases and have been budded onto seedling rootstock for further evaluation.

The North American pawpaw [Asimina triloba (L.) Dunal] is a native tree-fruit that is in the early stages of commercial production in the United States. Pawpaw fruit have fresh market appeal for farmers markets, community supported agriculture, and organic markets, as well as processing potential for frozen pulp production. For the processing market, fruit with a large size of over 120 grams and less than 6 percent seed by weight, are desirable for processing for pulp recovery for value-added products such as hot sauce. Kentucky State University has already released three pawpaw cultivars, KSU-AtwoodTM in 2009, KSU-BensonTM in 2016, and KSU-ChappellTM in 2018. However, new high yielding cultivars with excellent fruit quality would further assist in the development of the pawpaw industry. The objective of this study was to determine the fruit quality of 16 pawpaw cultivars and advanced selections. Fruit weight, percent seed, and brix were determined for the cultivars and advanced selections grown at the Kentucky State University Research and Demonstration Farm in Frankfort, Kentucky. The cultivars and advanced selections evaluated were A3-1, A6-1, KSU-AtwoodTM, KSU-BensonTM, KSU-ChappellTM, G4-25, G6-120, G9-109, Hi 1-4, Hi 7-1, Hy3-120, NRVT 3-10, NRVT 3-4, Sunflower, Susquehanna, and Tallahatchie. Average fruit weight ranged from Tallahatchie at 138g to A-6-1 at 277g per fruit. A number of advanced selections had large fruit size and these included A6-1 (277g), Hy3-120 (258g), A3-1 (216g), Hi1-4 (214g), NRVT3-4 (208g), Hi7-1 (202g) and G9-109 (201g). Average percent seed for fruit ranged from G6-120 at 8.2 percent seed to the cultivar KSU-ChappellTM at 4.5 percent seed. A number of advanced selections had low percent seed and these included A6-1 (4.7%) and G4-25 (5.8%). Average percent Brix for fruit ranged from NRVT 3-10 at 19.8 Brix to the cultivar KSU-ChappellTM at 28.2 Brix. Many of these advanced selections show promise as potential new cultivars. Examples of hot sauce made from pawpaw pulp extracted from cultivars will be discussed.

As global warming progresses, sunburn damage in fruit production is becoming increasingly prevalent. However, there has not been much research on the patterns of sunburn occurrence and effective prevention measures for ‘Fuyu’ sweet persimmons. This pilot study explored the impact of strong light and high temperatures on sunburn occurrence in ‘Fuyu’ sweet persimmons, characterized the temporal pattern of sunburn development, and evaluated the efficacy of chemical suppressants in reducing damage. ‘Fuyu’ persimmons were harvested from an orchard in Jinju-si, Gyeongsangnam-do, South Korea, at 60 days (weak light intensity (WLI) conditions), 72 days (strong light intensity I (SLI-I) conditions), and 79 days (strong light intensity II (SLI-II) conditions) after full bloom. The harvested fruits were divided into five treatment groups: control (Distilled water), 2% Cellulose Nano Crystals (CNCs), 2% Sodium Alginate 300mM CaCl2 (AG), 6% Kaolin 0.1% Tween20 (K), and 2% Vapor Gard® (VG, transpiration inhibitor). Thermocouples were attached to each fruit to measure fruit surface temperature (FST) under the following conditions: WLI (using a chamber set to 200 µmol/m²/s light intensity) and SLI-I, -II (direct sunlight and temperature) conditions. When exposed to WLI conditions, the increase in fruit surface temperatures (FST) compared to air temperatures (AT) was minimal, and no sunburn was observed. Conversely, non-acclimated fruits exposed to SLI-I displayed extensive sunburn damage within a day. However, acclimated fruits under SLI-II revealed differences in sunburn. The kaolin treatment, which exhibited the lowest browning, also showed a significant reduction in FST. However, the carotenoid content was lower in the kaolin treatment compared to the control. To confirm the findings from controlled experiments, field trials were conducted for two years to evaluate the effectiveness of sunburn suppressants. In the first year, kaolin treatment reduced the severity of sunburn by half compared to other chemical suppressants. In the second year, 6% kaolin and a mixture of kaolin and carnauba wax were applied. Both kaolin and a kaolin-carnauba wax mixture effectively reduced sunburn to 30% of the control. These effective treatments decreased FST by up to 3.5℃, maintained high chlorophyll content, and augmented antioxidant enzyme activities. In conclusion, the kaolin treatment combined with carnauba wax was identified as the most promising material for preventing sunburn damage in ‘Fuyu’ persimmons under these environmental circumstances.

The wood decay fungus, Ganoderma adspersum, with no prior record in North America has emerged as a pathogen of almond in the San Joaquin Valley within the past decade. Incidence of the disease appears highest in orchards on ‘Nemaguard’ rootstock with high levels of crown gall, a bacterial disease caused by Agrobacterium tumefaciens. The putative association of G. adspersum with crown gall has been hypothesized, but not formally evaluated in the field. In February 2024, over 6,000 trees were surveyed across two orchards and rated for incidence of each disease. Both orchards were on ‘Nemaguard’ rootstock and included ‘Nonpareil,’ ‘Monterey,’ and ‘Fritz’ varieties. Results of Chi-square test for binomial ratings of two categorical variables indicate a significant association between G. adspersum and crown gall infection on all varieties in both orchards. Furthermore, when the variables were fitted using the generalized linear mixed model, infection with G. adspersum (independent variable) was found to have a significant effect on the association (response variable) for ‘Nonpareil’ variety at one site. For each unit increase in the incidence of crown gall, the probability of infection with G. adspersum increased by more than 1×109 fold on ‘Fritz’ and ‘Monterey’ at both sites, and ‘Nonpareil’ at one site, and by more than 87-fold on ‘Nonpareil’ at the second site. The results support the hypothesis that crown gall predisposes almonds to infection by G. adspersum. Further studies are needed to address the mechanism by which crown gall infection predisposes almond to G. adspersum with the long-term goal of improving the economic lifespan of California almond orchards.

In October 2020, the Republic of Korea set a landmark goal to achieve net zero emissions by 2050. However, the national greenhouse gas emission statistics did not account for carbon absorption by agricultural lands, including orchards, due to the absence of adequate calculation systems and data. Recognizing the significant role of photosynthesis in fruit trees like Citrus in carbon sequestration and climate change mitigation, this study aims to address this gap. We focused on developing allometric equations for estimating biomass and carbon stocks of citrus trees, particularly in orchards across Korea, with a case study on Jeju Island, a principal citrus cultivation area. Measurements of height, crown width, and the diameter of rootstock and trunks were conducted, observing an increase with tree age, though trunk diameter showed considerable variability. The study encompassed trees aged 5–45 years of the Satsuma Mandarin (Citrus unshiu Marc. cv. Miyagawa), a major citrus cultivar cultivated in Korea. Components including leaves, branches, trunks, and roots were harvested, dissected, fresh weighed, and oven-dried to develop dry to fresh weight ratios for dry biomass calculation. Allometric equations with trunk diameter as the sole independent variable were formulated for the Satsuma Mandarin tree. Furthermore, the total carbon content for trees aged 5–45 years was assessed through direct and destructive harvesting, revealing a carbon content range of 44.13 to 46.85% across different tree organs, with root carbon content increasing with age. This comprehensive study provides crucial insights into the biomass and potential carbon fixation capabilities of citrus trees, contributing significantly towards the accuracy of national carbon stock estimations. This work was carried out with the support of the Research Program for the National Institute of Horticultural

Tropical crops have a tremendous impact on global food systems and are well represented in Hawaii. There are many different diseases (bacterial/viral/fungal) that can impact yields. In this session, we invite an audience from a broad pool of research interests and backgrounds to attend and learn about a range of food and forestry crops that are important in Hawaii, including papaya, sweetpotato, the native koa tree and banana and the production systems unique to these crops. The wide range of crops from tuber, to fruit, to forestry has led to a variety of challenges that have attracted less attention compared to pathology problems associated with temperate crops. Short topic presentations in these areas will begiven by and from the perspective of university, non-profit institution, and USDA ARS researchers and a local commercial grower followed by discussions between speakers and individual break out groups consisting of workshop attendees. Attendees will have the opportunity to sample a few varieties of tropic fruit crops and view items made from koa. Through these talks and interactive discussions, we aim to convey the scope of tropical diseases that occur, successful strategies to overcome them given different crop-specific constraints and highlight their relevance to research on emerging problems impacting agriculture in temperate and other regions due to climate and environmental changes.
Coordinator(s)

Dr. Jon Suzuki, USDA ARS DKI U.S. PBARC, Hilo, HI, United States
Dr. Michael Kantar, Associate Professor, Department of Tropical Plant and Soil Sciences, University of Hawaii at Manoa,, Honolulu, United States

Participants:

Dr. Nathan Fumia (HARC - Hawaii Agricultural Research Center) – Understanding the role of selection on fungal disease tolerance in Acacia koa, an endemic Hawaiian hardwood (8 minutes).
A common occurrence during neo-domestication is the interplay of traits such as seedling vigor and disease resistance. The focus of this study is the effect of crossing on a wild species being selected for a domesticated form, including decisions such as number of parents, number of crosses, and number of progeny. Our aim is to begin breeding scheme development for wild species (neo-domestication) using Acacia koa as a novel study system. Breeding population size was changed in the koa orchard through thinning of individuals, a form of pollen control in the population, with the major criterion being a low durability of resistance where trees succumb to disease over time, caused by Fusarium oxysporum. This is an opportunity for insight during the development of expectations in population improvement through augmented crossing parameters, informing situational changes through breeding cycles in neo-domestication programs.
Dr. Anna McCormick (University of Hawaii at Manoa) – Examining viral presence in collections of Hawaiian heirloom sweetpotato varieties (8 minutes).
Sweetpotato has been a staple crop in the Hawaiian Islands since Polynesians settled the islands ~1,200 years ago. Previous work has established that Hawaiian Heirloom sweet potato varieties represent unique germplasm that are more closely related to each other than varieties from other parts of the world. While much work has been done characterizing Hawaiian heirloom plant genetics, much less work has been done characterizing viral genetic presence and diversity. In this study we explored viral presence across an extant collection of 70 Hawaiian heirloom varieties sourced from five different botanical collections over three Islands (O’ahu, Maui and Big Island Hawai’i). Samples were examined for the presence of four viruses from the family Potyviridae namely; Sweet potato virus G (SPVG), Sweet potato virus C (SPVC), Sweet potato virus 2 (SPV2), Sweet potato feathery mottle virus (SPFMV), and one from the family Geminiviridae; Sweet potato leaf curl virus (SPLCV). We found high levels of virus across all collections, including many instances of double and triple infections. These findings have large implications for conservation and local fresh markets.
Dr. Lorin Harvey (Mississippi State University) – Research and discovery of best practices for U.S. sweetpotato clean foundation seed (CFS) programs. (8 minutes)
Sweetpotato is an important crop for the U.S. and worldwide. Infection by viruses which accumulate over time in this and numerous other major perennial crops cause major yield losses annually. The problems in sweet potato are being addressed through Sweetpotato CleanSEED, a multi-state, transdisciplinary funded research project funded by a USDA-NIFA-SCRI grant to develop strategies that improve sweetpotato crop health and yield through developing quality control standards for producing clean foundation seed, improved detection methods, identification of undiscovered viral diseases, and development of cultural practices for efficient clean foundation seed production in both the laboratory and greenhouse to reduce disease transmission in this clonally propagated crop.
Ryan Domingo (USDA ARS U.S Pacific Basin Agricultural Research Center) - Identifying Carica papaya cultivars demonstrating increased partial resistance towards Phytophthora palmivora utilizing a novel rapid phenotyping assay. (8 minutes)
Extensive production loss and mortality of Carica papaya is associated with Phytophthora palmivora in Hawaii and other papaya growing regions of the world. Currently there is no commercial papaya cultivar fully resistant against P. palmivora, however it is important to identify those that contain partial resistance reducing pathogen proliferation. This talk will explore a novel rapid phenotyping assay quantifying P. palmivora growth along papaya seedling stems comparing several genotypes within the USDA germplasm collection. Implications resulting from this study provides farmers, breeders and other stakeholders insight towards cultivars displaying increased partial tolerance towards P. palmivora.
Gabriel Sachter-Smith (Hawaii Banana Source) - Maintaining commercial vitality of the banana industry through germplasm resources and strategies to mitigate disease (8 minutes).
Bananas have been grown in Hawaii since the first waves of Pacific Islander migrations starting over 1000 years ago. Today, bananas represent one of the most widely consumed fresh produce items in Hawaii, despite a steady downward trend in local production since the introduction of Banana bunchy top virus (BBTV) in 1989. To date, no commercially viable cultivar with strong resistance to BBTV has been identified or created, and various ways to combat the virus used in other parts of the world have proved challenging to apply in Hawaii. However, the pressure of the virus and overall impact on a farm can be mitigated using a combination of cultural practices and semi-tolerant cultivars. Through the use of frequent crop rotations, on-farm generated clean plant systems, and working with buyers to adapt alternative varieties to new markets, banana production can be maintained and improved in Hawaii.

Competition Entry https://ashs.org/page/SIEntry

The Graduate Student Activities Committee hosts this competition to allow graduate students members (both MS and PhD) a fun and exciting way to communicate the impact of their research projects and scholarly activities.
Graduate students make compelling presentations in three minutes using no more than one single PowerPoint slide or visual aid. The purpose of the presentation is to generate awareness, stimulate thought, and inspire attendees to appreciate the breadth and depth of horticultural research and scholarly activities. Graduate students will meet other students and scientists, learn about an array of research topics and other creative and scholarly activities across ASHS, and most importantly have fun!

The competition is designed similar to other popular three to five minute thesis and research presentation events (e.g. Three minute thesis, ‘Ignite’ or Scholars’ Studio) occurring around the globe, and done especially well in Australia, British Columbia, and the University of Washington.

Winners will receive a cash award of $500, $250, and $100 for the top three places, respectively.
The winners are announced at the Student Awards Night.
Students must enter by the deadline of Sept 1st. 
Applicants may use only one Introductory Slide (using the ASHS provided template) and one Presentation Slide for a total of 2 slides. The Introductory Slide must follow the format of the template and include Title of Presentation (Calibri 44 bold), Student’s Name (Calibri 32 bold), Advisor(s) Name (Calibri 28) and Institution’s Name (Calibri 28). The Presentation Slide must be one single page without any animation. Students may use any color, background or font for their Presentation Slide but not for the Introductory Slide. Slides need to be in the 16:9 format. Both slides must be presented in 3 minutes. Once the student reaches the podium the timer will start and after 3 minutes we will move to the next student.