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Wednesday, September 25
 

7:00am HST

Speaker Ready Room - Wednesday
Wednesday September 25, 2024 7:00am - 5:00pm HST
Wednesday September 25, 2024 7:00am - 5:00pm HST
Sea Pearl 1-2

7:30am HST

Extension Division Breakfast and Education Materials Awards Celebration
Wednesday September 25, 2024 7:30am - 9:30am HST
Speaker: Dr. Carol Miles, Washington State University
 
Extension Outreach in a Digital World
 
Presentation description: As Extension specialists we carry out applied research and develop new research-based information and deliver it to our clientele in easy-to-understand publications. Today we must deliver our information on-line and though social media, and we track numbers of hits and downloads. How have Extension publication trends changed over the past 20-30 years, including print/online, sales/revenue, promotion/advertising, and what do we see as the future for extension publications. Forming partnerships with extension communication specialists can enable us to focus on information development, where most extension specialists do best, while achieving impacts through information access, which is how we are evaluated.
Moderator
AN

Ajay Nair

Iowa State
Speakers
CM

Carol Miles

Washington State University
Wednesday September 25, 2024 7:30am - 9:30am HST
Hibiscus

7:30am HST

Registration Open - Wednesday
Wednesday September 25, 2024 7:30am - 5:00pm HST
Wednesday September 25, 2024 7:30am - 5:00pm HST
Coral Lounge

7:59am HST

Plasticulture (PLAST)
Wednesday September 25, 2024 7:59am - 9:00am HST
Cover Crop Windbreaks for Slowing Biodegradable Mulch Deterioration and Improving Bell Pepper Productivity. - Caleb Wehrbein
Influence of Plastic Mulch Color on Yield and Quality of Day-neutral Strawberries - Tricia Jenkins
Exploring sustainable mulch solutions: a comparative study in strawberry production systems in northwestern Washington - Nayab Gull
Shade level effects on fruit yield of habanero chili (Capsicum chinense Jacq.) - Juan Carlos Diaz Perez
Moderator
avatar for Juan Carlos Diaz Perez

Juan Carlos Diaz Perez

Professor of Horticulture, University of Georgia
Wednesday September 25, 2024 7:59am - 9:00am HST
Lehua Suite

7:59am HST

Plant Growth Regulation 1 (PGR 1)
Wednesday September 25, 2024 7:59am - 9:30am HST
Evaluation of Plant Growth Regulators on Sweetpotato Slip Propagation - Kerington Bass
Effects of a Cytokinin-Containing Biostimulant Applied at Different Phenological Timings on Almond (Prunus dulcis) Yield - Orlando Tapia
The Application of a Cytokinin B-Mo-based Product Influences the Source-to-Sink Dynamics and Non-Structural Carbohydrate - Mayra Toro Herrera
PGR applications to reduce HLB-associated preharvest fruit drop in Sweet Orange - Tripti Vashisth
Ethephon Effect on Blooming of Three Southern Highbush Cultivars - Lilian Carcamo
Development of a Tissue Culture Approach for Doubling the Ploidy Level of Southern Highbush Blueberry Varieties - Emily Walter
Moderator
avatar for Kerington Bass

Kerington Bass

Graduate Student, Mississippi State University
Originally from Louisiana Nontraditional ag background Interested in horticulture of specialty crops or ornamentals Highly interested in Controlled Environment Agriculture Looking for job opportunities Looking for opportunities to learn a second language 
Wednesday September 25, 2024 7:59am - 9:30am HST
South Pacific 3

7:59am HST

Produce Quality, Safety, and Health Properties (QUAL)
Wednesday September 25, 2024 7:59am - 9:30am HST
The Effect of Fruit Tree Virus and Viroid Infections on Tree Growth and Fruit Quality - Sang Yun Cho
Bacteriophage as an Alternative Method to Control Salmonella enterica in Water-Recirculated Systems for Lettuce Production - Camila Rodrigues
Transfer of Escherichia coli from Plastic Mulch to Tomato and Pepper Fruit by Ground Contact in a Field Environment - Autumn Burnett
Assessing the Impact of Biological Soil Amendments of Animal Origin on Bell Pepper Microbial Safety, Soil Properties, and Quality - Daniel Leiva
Inhibiting Foodborne Pathogens and Biofilms on Fresh Produce by Nano-emulsions Developed from Lactobacillus-Derived Exopolysaccharide and Eugenol - Sangeeta Balyan
Evaluating Nutritional Quality and Consumer Acceptability of Lettuce (Lactuca sativa L.) Grown with a Movable High Tunnel - Cary Rivard
Moderator
SY

Sang Yun Cho

agricultural researcher, National Institute of Horticultural and Herbal Science
Wednesday September 25, 2024 7:59am - 9:30am HST
Kahili

7:59am HST

Citrus Crops 2/History of Horticultural Science (CITR 2/HIST)
Wednesday September 25, 2024 7:59am - 9:45am HST
Precision Nutrition Management: Finding Optimal Methods for Leaf Nutrient Analysis in HLB-affected Citrus Trees - Meryam Manzoor
Cover Crop Effects on HLB-affected Citrus Tree Growth and Soil Characteristics: Results From a 3-year Field Trial - Lorenzo Rossi
More Frequent Irrigation Increases Yield in HLB-Affected Sweet Orange - Tripti Vashisth
Impact of Different Organic Matter Contents on ‘US-942’ Citrus Rootstock Physiology, Nutrient Uptake, and Root Morphology - Lukas Hallman
Soil Amendment Strategies to Rehabilitate Freeze Damaged Citrus Trees for Sustainable Crop Management - Shad Nelson
Physiological and Metabolomic Changes in Young Citrus Trees Under Elevated CO₂ and High Temperatures: A longitudinal study in Mandarins (C. reticulata.) - Taylor Hornburg
Reports and research on incorporating cover crops in Florida citriculture, 1896 – 2024 - Lorenzo Rossi
Moderator
avatar for Lorenzo Rossi

Lorenzo Rossi

Associate Professor, University of Florida
Dr. Rossi’s research program focuses on improving root health and growth on cultivated crops, leading to the development of environmentally sound and effective management methods. He is a horticulturist with specific expertise related to plant stress physiology, root dynamics and... Read More →
Wednesday September 25, 2024 7:59am - 9:45am HST
South Pacific 1

7:59am HST

Floriculture 1 (FLOR 1)
Wednesday September 25, 2024 7:59am - 9:45am HST
Phosphate Solubilizing Bacteria: Leveraging The Soilless Substrate For Improved Phosphorus Nutrition In Controlled Environment Agriculture. - Michelle Jones
Phosphate Solubilizing Bacteria Isolated From Greenhouse Ornamentals Solubilize Phosphate In Vitro And Ameliorate P Deficiency Symptoms In Marigold ‘Durango Yellow’ Fertilized With Calcium Phosphate. - Juan Quijia Pillajo
Activated Aluminum Amended Substrates Reduce Phosphorus Leaching in Floriculture Production - Damon Abdi
Iron-coated Sand as a Sustainable Substrate Amendment for Nutrient Management of Containerized Floriculture Crops - Savannah Mead
Phosphorous Restriction Suppresses Growth of Containerized Coneflower - Garrett Owen
Investigating Synergistic Effects of Biostimulants and Biochar on Water Use Efficiency for Containerized Celosia (Argentea cristata) and Gomphrena (Gomphrena globose) Production - Lilin Chen
Effects of Reduced Water Usage During Production on Economic Value, Growth, And Quality of Flowering Indoor Plants - Kaitlin Hopkins
Moderator
DA

Damon Abdi

Louisiana State University Agricultural Center
Wednesday September 25, 2024 7:59am - 9:45am HST
Nautilus

7:59am HST

Genetics and Germplasm 1 (GG 1)
Wednesday September 25, 2024 7:59am - 9:45am HST
Assembling a Reference Panel of DNA Profiles for U.S. Heirloom Apple Cultivars - Cameron Peace
Rediscovering Lost Heirloom Apple Cultivars with DNA Fingerprinting - Dongyun Lee
Air or Soil Temperature: Understanding the Cues for Dormancy Transition in Peach - Ksenija Gasic
Selection and Evaluation of Citrus Resistobiome for HLB Resistance/Tolerance - Yongping Duan
Population Genetics and Genome-wide Association Studies Provide Insights into the Genetic Basis of Persea Fruit Quality Traits - Gul Ali
Genome Assembly of Persia Americana cv. Simmonds Provides Insights on Genetic Relationships Among Avocado Hybrids Exhibiting Tolerance To Laurel Wilt - Vincent Njung'e Michael
Utilizing Haploid Pollen Grains and Diploid Leaf Tissue Genomic Sequence Data to Phase the ‘Wonderful’ Pomegranate Genome - Giuseppe Lana

Moderator
CP

Cameron Peace

Washington State University
Wednesday September 25, 2024 7:59am - 9:45am HST
Coral 1

7:59am HST

Technology Applications in Horticulture 1 (TECH 1)
Wednesday September 25, 2024 7:59am - 9:45am HST
Advanced 3D Imaging for High Throughput Phenotyping of Horticultural Crops - Yu Jiang
Integrating UAV Imagery and AI to Forecast Vidalia Onion Yield and Quality - Marcelo Barbosa
Deep Learning Application for Field Phenotyping of Shoot Structure in Grapevine - Soichiro Nishiyama
Investigation of Using Hyperspectral Vegetation Indices to Assess Brassica Downy Mildew - Bo Liu
Effect of Innovative Laser Labeling Technology on Fresh Produce Quality and Safety - Manreet Bhullar
Cover Crop Decision Support Tools: Exploring the new suite of online cover crop tools - Esleyther Henriquez Inoa
CFD-based aerodynamic analysis under high wind velocity environment for multiple greenhouses - Anthony Kintu
Moderator
SD

Shunping Ding

Associate Professor, California Polytechnic State University
Wednesday September 25, 2024 7:59am - 9:45am HST
South Pacific 2

7:59am HST

Growth Chambers and Controlled Environments 2 (CE 2)
Wednesday September 25, 2024 7:59am - 10:00am HST
Will Increasing the Potassium to Nitrogen Ratio Benefit Hydroponic Strawberry Production? - Jonathan Ries
Impact of Diurnal Chilling on Vegetative and Floral Development of Strawberry (Fragaria x annanassa) cvs. ‘Albion’, ‘Chandler’, ‘Monterey’, ‘Sensation’ Tray Plants in a Controlled Environment - Michael Palmer
Utilizing Controlled Environment Agriculture to Enhance the Yield and Flavor of Strawberries - Nicholas Cooley
Impact Of Elevated CO2 And Two Daily Light Integrals on the Production Efficiency of Strawberry (Fragaria × ananassa ‘Monterey’) Daughter Plants - Samson Humphrey
Unraveling Strawberry Stock Plant Architecture, Morphology, and Tips’ Spatial Distribution under Three Photoperiods to Facilitate Future Propagation System Design: A Comparative Study on Monterey as a Long-day Cultivar - Moein Moosavi
Stratified Wood Substrates for Optimizing Growth of Greenhouse-Grown Strawberries and Blueberries - Brandan Shur
Effect of Light Intensity on Strawberry Runner Tips Propagated Indoors - Lian Duron
Characterizing the Growth, Morphology, Productivity, and Fruit Quality of Twenty-five Strawberry Cultivars in Vertical Farm Environment - Yiyun Lin
Moderator
YL

Yiyun Lin

The Ohio State University
Wednesday September 25, 2024 7:59am - 10:00am HST
Coral 2

7:59am HST

Vegetable Crops Management 2 (VCM 2)
Wednesday September 25, 2024 7:59am - 10:00am HST
Optimizing Tomato Crop Production: Fertility Management in Soilless Media - Bryce Waugh
Improving the Growth and Yield of Tomato through new Integrated Pest Management Strategies - Rosemary Gutierrez Coarite
Assessment of Novel, Interspecific Hybrid Tomato Rootstocks for Production under Environmental Stress - Joshua Harvey
Evaluation of Biostimulant on Nutrient Use Efficiency in Tomato - Weining Wang
Alternative Carbon Sources Effects on the Soil-plant Nutrient Dynamics During and Post ASD in a High Tunnel Tomato Crop System - Francesco Di Gioia
Advancing Pepper Production Beyond Seasonal Limits: Findings from a High Tunnel System Integrated with Biostimulant Applications - Asmita Nagila
Optimizing Nitrogen Fertilizer Rate and Planting Density of Vidalia Onion Yield and Bulb Size Distributions - Elvis Pulici
Assessing the Efficacy of Plant Biostimulants on Onion Growth and Production in Greenhouse Trials and Field Conditions - Prakriti Nepal
Moderator
FD

Francesco Di Gioia

Pennsylvania State University
Wednesday September 25, 2024 7:59am - 10:00am HST
South Pacific 4

8:00am HST

CE 2 - Will Increasing the Potassium to Nitrogen Ratio Benefit Hydroponic Strawberry Production?
Wednesday September 25, 2024 8:00am - 8:15am HST
Strawberries (Fragaria × ananassa) are increasingly being grown in hydroponic systems, where effective nutrient management is critical for optimizing crop production and yield. Among essential mineral nutrients, potassium is a key nutrient that affect fruit quality in many fruiting crops. This study investigated how potassium to nitrogen (K:N) ratios regulate strawberry growth and development in a deep-water culture hydroponic system. We hypothesized that increasing the K:N ratios would enhance vegetative growth, fruit yield, and fruit quality. Bare-root plants of strawberry ‘Monterey’ and ‘San Andreas’ were grown indoor under a 23 °C air temperature and an 18-h photoperiod with an extended photosynthetic photon flux density of 350 µmol∙m–2∙s–1. The N level was kept constant at 77 ppm, while the study tested K:N ratios ranging from 1.5:1 to 4:5:1. Three weeks after nutrient treatments, strawberry plants showed similar crown number, crown diameter, leaf number, leaf area, and shoot dry mass regardless of K:N ratios in both cultivars. Increasing K:N from 1.5:1 to 4.5:1 linearly increased the root dry mass of Monterey’ but not in ‘San Andreas’. During the fruit production, K:N ratios did not affect the total number of fruits or total fresh mass of fruits. However, there was a linear decrease in the diameter, length, and fresh mass of individual fruits with increasing K:N ratio from 1.5:1 to 4.5:1 in both 'Monterey' and 'San Andreas'. Additionally, increasing K:N ratio from 1.5:1 to 4.5:1 slightly increased total soluble solids in ‘San Andreas’, but led to a greater increase in titratable acidity compared to the increase in total soluble solids in both ‘Monterey’ and ‘San Andreas’. These results suggest that increasing K:N ratios from 1.5:1 to 4.5:1 has little beneficial effect on overall plant growth, fruit yield and fruit quality for hydroponic strawberry production in deep-water culture systems.
Speakers
JR

Jonathan Ries

Arizona State University
Co-authors
YP

Yujin Park

Arizona State University
Wednesday September 25, 2024 8:00am - 8:15am HST
Coral 2

8:00am HST

CITR 2/HIST - Precision Nutrition Management: Finding Optimal Methods for Leaf Nutrient Analysis in HLB-affected Citrus Trees
Wednesday September 25, 2024 8:00am - 8:15am HST
Effective fertilizer management and accurate nutritional analysis are critical for healthy tree growth and maximum yield. Citrus trees perform best in the optimum range of each nutrient, and a deficiency or excess can result in poor tree growth and yield. Accurate fertilizer management decisions for citrus trees begin with proper leaf sampling. To accurately assess tree nutrition status, the appropriate time and procedure for collecting leaves for nutrient analysis must be followed. Historically, nutrient management for healthy citrus trees involved annual leaf sampling from July to August, with subsequent year fertilization plans based on single nutrient analysis. Currently, nearly all commercial Florida citrus orchards are affected by Huanglongbing (HLB), a systemic bacterial disease that affects all citrus varieties and rootstocks, causing extensive tree health decline. Studies suggest that a good nutrition program can improve the tree health and production of HLB-affected trees. However, the key is to supply the nutrients that are deficient or low. This widespread prevalence of HLB needs new approaches for managing fertilizer programs by regular leaf sampling to identify tree nutrient status and requirements. Thus, this study focuses to improve and revise the leaf nutrient sampling guideline for HLB-affected trees by determining the optimal time for leaf sampling and adjusting fertilization for increased production and tree health. Key objectives include assessing the frequency of leaf sampling required per year to capture the tree's nutritional status effectively and adjust fertilizer accordingly to establish the correlation of leaf nutrient concentration with the yield, fruit drop, and canopy density. For this study, spring and summer emerging flushes were tagged on HLB-affected ‘Hamlin’ and ‘Valencia’ mild and severe sweet orange trees in a central Florida orchard over two years. The leaves from tagged branches were collected every 3 months for leaf nutrient analysis and the results of both spring and summer leaves were used to adjust the fertilization plan accordingly. It was found that the spring emerging flush was optimal in adjusting the fertilizer for the highest yield compared to the summer emerging flush. With the use of precision fertilization, the improvement in tree canopy density and fruit yield was more obvious in mildly HLB-symptomatic trees compared to severely symptomatic trees. Our results demonstrate the growers should be performing nutrient sampling quarterly and use this information to precisely manage fertilization and improve tree productivity.
Speakers
MM

Meryam Manzoor

University of Florida
Co-authors
JB

Jamie Burrow

University of Florida
NA
TL

Taylor Livingston

University of Florida
NA
TV

Tripti Vashisth

University of Florida
Wednesday September 25, 2024 8:00am - 8:15am HST
South Pacific 1

8:00am HST

FLOR 1 - Phosphate Solubilizing Bacteria: Leveraging The Soilless Substrate For Improved Phosphorus Nutrition In Controlled Environment Agriculture.
Wednesday September 25, 2024 8:00am - 8:15am HST
Phosphorus (P) is an essential macronutrient with low availability for plant uptake. The availability of P is reduced by the formation of insoluble complexes with calcium (Ca) and iron (Fe). Phosphorus solubilizing bacteria (PSB) can enhance P uptake by producing organic acids that acidify the rhizosphere and breakdown the insoluble P compounds. The goal of this research is to identify PSB that can increase P uptake efficiency in soilless production systems. A collection of bacteria isolated from the rhizosphere of greenhouse grown ornamentals was used to identify PSB using both in vitro and in planta evaluations. A malachite green assay optimized for 96-well plates was used to screen 1,056 bacterial isolates for the ability to solubilize phosphorus from both calcium phosphate and iron phosphate. This in vitro assay identified 14 and 24 PSB that solubilized 25% or more of the P from Ca2(PO4)3 and FePO4·2H20, respectively. There was no overlap between the PSB that solubilized Ca2(PO4)3 and those that solubilized FePO4. In planta evaluations were conducted in Marigold ‘Durango Yellow’ (Tagetes patula) and Tomato ‘Bush Beefsteak’ (Solanum lycopersicum) grown in a peat-based substrate (pH=7) and inoculated with individual PSB as a media drench (20 mL; OD595=0.1). Digital phenotyping with the TraitFinder (Phenospex) was used to quantify growth promotion (digital biomass and bloom area), and the severity of P deficiency symptoms [Green Leaf Index (GLI), normalized difference vegetation index (NDVI), Normalized Pigment Chlorophyll Ratio Index (NPCI), and Plant Senescence Reflectance Index (PSRI)]. Only seven FePO4·2H20 solubilizers (Fe-PSB) and six Ca2(PO4)3 solubilizers (Ca-PSB) performed better than the control in at least one of the evaluated parameters. A validation experiment was conducted to confirm the beneficial activity of the seven Fe-PSB (C2F9, C3A8, C11G1, C8D10, C6E7, C3G9, and C3F10) and the six Ca-PSB (C4A1, C2G6, B3A7, C11A5, C4B6, C12F4, and C6H6). The validation experiments were conducted with Marigold ‘Durango Yellow’ and Tomato ‘Early girl’ grown in a peat-based substrate (pH=7) and inoculated with individual PSB as a media drench (20 mL; OD595=0.1). Bacillus megaterium (C3F10), Pseudomonas sp. (C6E7), and two strains of Pantoea rwandensis (C3A8 and C8D10) showed a beneficial response when applied to plants fertilized with FePO4·2H20. Only, Enterobacter soli C4A1 showed a beneficial response when applied to plants fertilized with Ca2(PO4)3. The malachite-green assay and digital phenotyping are suitable tools for high-throughput identification of PSB that can be used to improve phosphorus nutrition in soilless culture systems.
Speakers
MJ

Michelle Jones

Professor, The Ohio State University
Co-authors
JQ

Juan Quijia Pillajo

The Ohio State University
LC

Laura Chapin

The Ohio State University
NA
SN

Sachin Naik

The Ohio State University
NA
Wednesday September 25, 2024 8:00am - 8:15am HST
Nautilus

8:00am HST

GG 1 - Assembling a Reference Panel of DNA Profiles for U.S. Heirloom Apple Cultivars
Wednesday September 25, 2024 8:00am - 8:15am HST
Heirloom apple cultivars represent an important specialty crop for producers and a genetic resource for the dessert apple industry. These cultivars are plagued with misidentifications, which hinders utilization and long-term preservation. Phenotypic identification, used for centuries, is unable to distinguish among the thousands of existing U.S. heirlooms. DNA profiling provides an objective basis for cultivar identification. Washington State University’s “MyFruitTree” (myfruittree.org), built upon the RosBREED project and with international collaborations, has accumulated a DNA profile dataset of thousands of apple individuals focused on the U.S. genepool. Users submit leaf samples for trees of interest, and MyFruitTree’s cost-recovery research opportunity determines the cultivar identity (or reveals their uniqueness and pedigree position). However, a common and valid question is, “How do you know that is the correct identity?” The core panel of robustly identified cultivars was based on public breeding germplasm, modern cultivars, and their ancestors, which were DNA profiled in the RosBREED project last decade. Since then, examined trees from collections in the U.S. and abroad have greatly expanded the number of DNA profiles with cultivar labels. But those labels are not always correct, especially when a tree is derived from only a single source. Therefore, a system was derived for assigning confidence to the cultivar labels associated with DNA profiles. Cultivar name evidence is assembled in the categories of provenance, phenotype, and genotype for both the DNA-profiled tree and the historically named cultivar, and congruence is examined. This system is applied at two levels: streamlined and comprehensive. The streamlined approach for the current DNA profile dataset efficiently determined whether each individual belongs confidently in the “Reference Panel” or is relegated to “Accessory Profiles” pending further evidence. A Reference Panel was assembled of hundreds of U.S. heirlooms (and more than a thousand close relatives from other regions). The comprehensive approach involves attention from a transdisciplinary tribunal of experts who carefully weigh evidence that an apple individual – often a proposed new discovery of an otherwise lost heirloom – is indeed a historically named cultivar. As MyFruitTree accumulates more DNA profiles submitted by cultivar collection managers and apple enthusiasts nationwide, the cultivar name assignment system is being applied to unidentified trees with strong provenance evidence of cultivar status. Establishing accurate cultivar identities of valued trees via DNA profiling is providing the critical foundation for a coordinated national effort to sustain preservation and utilization of apple crop diversity.
Speakers
CP

Cameron Peace

Washington State University
Co-authors
DL

Dongyun Lee

Washington State University
Wednesday September 25, 2024 8:00am - 8:15am HST
Coral 1

8:00am HST

PGR 1 - Evaluation of Plant Growth Regulators on Sweetpotato Slip Propagation
Wednesday September 25, 2024 8:00am - 8:15am HST
Producing sweetpotatoes involve several phases presenting unique challenges, most notably during transplanting. Sweetpotato slips, used for asexual reproduction, have non-uniform characteristics, making transplanting difficult. Additionally, the transition from a greenhouse to a field condition poses environmental risks and challenges, resulting in low transplant survival rates. A high slip mortality rate creates economic and logistical problems for producers. Plant growth regulators (PGRs) have been found to induce lignification or thickening of cell walls, which can alleviate environmental stresses in other crops; however, their impact remains unexplored in sweetpotato slips. Therefore, a study at Mississippi State University was conducted with the primary goal to enhance sweetpotato slip quality and improve transplant establishment rates. The study involves two greenhouse trials to identify the most effective PGR types and concentrations, and two histology trials to illustrate the effects of PGRs on sweetpotato slip lignin and cell wall. The greenhouse trials utilize a randomized complete block design to assess four different PGR types and thirteen concentrations on three replications of sweetpotato slips with 38 subsamples per treatment per replication. Data on plant height, stem diameter, number of nodes, SPAD, leaf area, and dry weight and fresh weight of the slips and roots were collected to determine the effects of PGRs on the plants. The histology trials included the same PGR treatments and concentrations to the slips. The slips were then collected, cross-sectioned, stained, and evaluated and measured under a microscope. The study proved that PGRs do have an effect on sweetpotato slips, by altering plant height, dry weight, and fresh weight, while not impacting the number of nodes. Thickening of the cell wall was also observed in slips treated with certain PGRs and it is hypothesized that this thickening of cell walls can contribute to reduced slip mortality when transplanting greenhouse produced slips to the field.
Speakers
avatar for Kerington Bass

Kerington Bass

Graduate Student, Mississippi State University
Originally from Louisiana Nontraditional ag background Interested in horticulture of specialty crops or ornamentals Highly interested in Controlled Environment Agriculture Looking for job opportunities Looking for opportunities to learn a second language 
Co-authors
BG

Bi Guihong

Mississippi State University
LH

Lorin Harvey

Mississippi State University
NA
avatar for Richard Harkess

Richard Harkess

Mississippi State University
NA
TB

Taylor Blaise

Mississippi State University
NA
Wednesday September 25, 2024 8:00am - 8:15am HST
South Pacific 3

8:00am HST

PLAST - Cover Crop Windbreaks for Slowing Biodegradable Mulch Deterioration and Improving Bell Pepper Productivity.
Wednesday September 25, 2024 8:00am - 8:15am HST
Polyethylene mulch films have become a dominant production practice to increase the yield potential of high value crops, but they are a significant source of environmental contamination. Alternatively, biodegradable mulches are a potentially sustainable alternative , but are less popular among growers as they vary in durability and performance in the field environment. Cover crops when sufficiently tall may be able to improve biodegradable mulch durability by reducing wind intensity within a mulched bed. To quantify the utility of a cover crop windbreak, we tested the effect of a fall-planted, 6ft wide strip of cereal rye planted parallel to the southern side of mulched bell pepper beds. Whole plot treatments included presence or absence of rye and split plot treatments included a comparison of polyethylene mulch, biodegradable mulch, and a bare ground control. Day windspeeds were reduced in peppers protected by a south-facing cereal rye strip by 60% relative to unprotected peppers. Large hole occurrences in biodegradable mulch were reduced by 50% and small holes by 15% in peppers protected by a cereal rye strip relative to unprotected peppers. Peppers grown behind the cover of cereal rye had a 42% reduction in leaning plants compared to peppers without cereal rye. Stomatal conductance was increased by 27% in peppers protected by cereal rye relative to unprotected peppers as a result of reduced windspeeds, though there were no differences in fruit yields. Results suggest cover crops can extend the useful life of biodegradable mulch films, which may help address a common barrier to adoption. Ongoing research aims to better understand the effects of the cover crop on crop health, yield, and quality.
Speakers
CW

Caleb Wehrbein

University of Nebraska - Lincoln
Co-authors
SW

Samuel Wortman

University of Nebraska - Lincoln
Wednesday September 25, 2024 8:00am - 8:15am HST
Lehua Suite

8:00am HST

QUAL - The Effect of Fruit Tree Virus and Viroid Infections on Tree Growth and Fruit Quality
Wednesday September 25, 2024 8:00am - 8:15am HST
This study aimed to investigate the impact of virus and viroid infections on tree growth and fruit quality attributes in apple, pear, peach, and grape. Trials were conducted in 2019 at the National Institute of Horticultural and Herbal Science in Korea, utilizing virus-infected, viroid-infected, combined virus/viroid-infected, and uninfected trees in an experimental fruit-tree orchard. Additionally, the experimental orchard served as an exhibition package for farmers and agricultural workers. Annual measurements of tree growth were compared between virus-free and virus/viroid-infected trees. Fruit quality attributes, including yield, weight, firmness, titratable acidity, and anthocyanin content, were assessed during 2021-2023. The results revealed significant differences between virus-free trees and those infected with virus, viroid, or both. Viral and viroid infections led to reduced tree height, trunk area, fruit yield, fruit weight, and anthocyanin content, while increasing fruit firmness and titratable acidity, respectively. These findings are expected to contribute to fruit tree virus and viroid disease control and fruit cultivation management. Furthermore, we anticipate that this research will enhance the understanding of agricultural stakeholders through scientific and comprehensive evidence on the damages caused by fruit tree viruses and viroid infections, thereby stimulating the adoption of virus-free stocks and promoting their widespread distribution.
Speakers
SY

Sang Yun Cho

agricultural researcher, National Institute of Horticultural and Herbal Science
Co-authors
BY

Byeonghyeon Yun

National Institute of Horticultural and Herbal Science
NA
HR

Hyun Ran Kim

National Institute of Horticultural and Herbal Science
NA
KH

Kang Hee Cho

Rural Development Administration
NA
SH

Se Hee Kim

National Institute of Horticultural and Herbal Science
NA
SO

Sewon Oh

National Institute of Horticultural and Herbal Science
NA
Wednesday September 25, 2024 8:00am - 8:15am HST
Kahili

8:00am HST

TECH 1 - Advanced 3D Imaging for High Throughput Phenotyping of Horticultural Crops
Wednesday September 25, 2024 8:00am - 8:15am HST
Understanding plant growth and development is crucial for insights into plant structure and function, and recent advancements in AI-driven 3D imaging technologies have revolutionized the acquisition and analysis of high-fidelity plant models. These technologies enable accurate and rapid measurement of phenotypic traits, aiding breeders in developing new varieties and helping horticulturists optimize production management. The overarching goal of this study was to establish an AI-based 3D imaging and analysis pipeline specifically designed for detailed examination of horticultural crops at the organ level within controlled environments. We developed a robotic platform equipped with a rotating base and a high-resolution camera mounted on a robotic arm, allowing comprehensive imaging from any angle around the plant. Utilizing this robot, we generated 3D models of 30 hemp plants from two growth-rate categories in controlled environments, on a weekly basis. An AI model was developed to segment these 3D models into stems, branches, and leaves. Morphological traits were extracted from each category of the segmented organs, including stem length (i.e., plant height), stem diameter, branch length, branch diameter, leaf number, leaf area, and leaf aspect ratio. These measurements contributed to a classification model capable of distinguishing between fast and regular growth rates. Experimental results showed that the 3D imaging-derived measurements were highly correlated with human-derived measurements. In addition, the extracted traits were used as quantitative descriptors to classify hemp cultivars with different growth rates in CEA. Therefore, the developed pipeline can be used as an effective and efficient tool for breeding programs and CEA production management in the future.
Speakers
YJ

Yu Jiang

Cornell University
Co-authors
JM

Jonathan Moon

Cornell University
NA
LS

Larry Smart

Cornell University
NA
NM

Neil Mattson

Cornell University
RD

Ruiming Du

Cornell University
NA
Wednesday September 25, 2024 8:00am - 8:15am HST
South Pacific 2

8:00am HST

VCM 2 - Optimizing Tomato Crop Production: Fertility Management in Soilless Media
Wednesday September 25, 2024 8:00am - 8:15am HST
Tomatoes are one of the most important greenhouse produced crops and the number of producers using greenhouse to grow them has risen in recent years. Sustainable development and effective resource management are becoming more and more important to the agricultural industries as well. Applying fertilizer precisely is becoming more and more important in different agricultural systems. Different physical and chemical characteristics of soilless growing substrate result in varying capacities for retaining nutrients. As a result, precise fertilizer rates are essential. This study investigated 14 fertilizer blends with varying proportions of nitrogen (0-400ppm), phosphorus (0-100ppm), and potassium (0-425). Six-cell seedling starter trays were filled with Berger BM6, and 'Big Beef' tomato seeds were planted. After four weeks, the seedlings were transplanted into five-gallon aeration fabric grow bags. Plants were hand-watered once a week with each treatment fertilizer rate to maintain a 10% leaching fraction. End measurements included the number of leaves, dry shoot weight, fresh root weight, dried root weight, and SPAD readings. The study revealed that a higher rate of nitrogen increased fresh shoot weight and lower rates of phosphorous increased number of non-harvestable fruits, increasing the need for further investigation to determine optimal fertilizer rates for various specialty crops grown in soilless greenhouse environments. Such endeavors are crucial for maximizing agricultural productivity while minimizing resource wastage and environmental impact.
Speakers
BW

Bryce Waugh

Grad Student, Oklahoma State University
Co-authors
BD

Bruce Dunn

Oklahoma State University
Wednesday September 25, 2024 8:00am - 8:15am HST
South Pacific 4

8:00am HST

Wearable Sensors to Support In-Situ Plant Health Monitoring
Wednesday September 25, 2024 8:00am - 9:15am HST
Speaker: Dr. Shawana Tabassum
Plants encounter different types of biotic (such as attacks by microbes, herbivores, invasive plants, and pests) and abiotic stresses (including drought, floods, salinity, extreme temperatures, and nutrient deficiencies) throughout their lifecycle. These environmental stresses lead to time-dependent changes in biochemicals, which circulate through the xylem and phloem, vary progressively, and can act as early indicators of plant stress. Key signal molecules produced in response to environmental stresses include both non-volatile phytohormones and volatile organic compounds. Therefore, accurate and timely measurement of these biomolecules would help producers and scientists diagnose crop stresses early, before visible symptoms appear, and optimize resources to minimize stress-induced growth and yield declines. This talk will focus on measuring these plant stress signals early with in-situ sensors.
Wednesday September 25, 2024 8:00am - 9:15am HST
Coral 5 - ASHS Hort Theater

8:00am HST

Exhibit Hall Hours - Wednesday
Wednesday September 25, 2024 8:00am - 2:00pm HST
Wednesday September 25, 2024 8:00am - 2:00pm HST
Coral 4/5 - Conference Central

8:00am HST

ASHS Conference Central - Wednesday Hours
Wednesday September 25, 2024 8:00am - 5:00pm HST
Resume Reviews - Tuesday September 24 - Friday September 27 -
Sign up at https://forms.gle/JQs6Nk6juM5vwfoaA
Reviewers Needed, Especially Industry
Sign-up at https://forms.gle/T65PqzTsBDepBR6k
Wednesday September 25, 2024 8:00am - 5:00pm HST
Coral 4/5 - Conference Central

8:15am HST

CE 2- Impact of Diurnal Chilling on Vegetative and Floral Development of Strawberry (Fragaria x annanassa) cvs. ‘Albion’, ‘Chandler’, ‘Monterey’, ‘Sensation’ Tray Plants in a Controlled Environment
Wednesday September 25, 2024 8:15am - 8:30am HST
In US open field nurseries, strawberry transplants for fruit production accumulate diurnal chilling hours (hours between -2 and 7°C) in the field prior to harvest and receive supplemental chilling as bare root plants in a cooler before being transplanted into a fruit production system. In nurseries in Northwestern Europe, tray plants are placed outside after rooting to accumulate diurnal chill hours and are moved into the cooler with leaves and substrate for supplemental chilling before being transplanted. The optimal amount of chilling varies among cultivars but is thought to contribute to a proper balance of vegetative vigor and floral development leading to best fruiting after transplanting into the production system. Climate change, labor cost and related issues cause problems in both nurseries and production. Therefore more and more growers and start-up companies venture into controlled environment production systems. Due to high cost, these systems require transplants that are optimally conditioned for early flowering. We evaluated the impact of diurnal chilling on vegetative and floral development of 250 cc strawberry tray plants in a controlled environment. 28 day old ‘Albion’, ‘Chandler’, ‘Monterey’, and ‘Sensation’ rooted daughter plants were moved into growth chambers where they received 0 (15°C 24 hours), 100 (4°C, 16°C), 250 (4°C, 19°C), or 450 (4°C, 24°C) chill hours over a six week period. Within each treatment, the same amount of chill hours were applied each day for the six week period. Among treatments, the non-chill temperature increased as the number of chill hours increased to ensure the same daily average temperature of 15°C. Chambers were fixed at 50% RH, 450 ppm CO2, 130-40 µmol m-2 s-1 for all treatments and a 12 hour photoperiod for ‘Chandler’ and ‘Sensation’ and a 16 hour photoperiod for ‘Albion’ and ‘Monterey’. After the treatment, all plants were dissected under the microscope to determine the number and development of floral meristems (flower mapping). Our results show that the plants in the 100 and 250 hour treatments produced more floral meristems and branch crowns than the 0 and 450 hour treatments across all cultivars. Additionally, in ‘Albion’ and ‘Monterey’, the plants of the 100 and 250 hour treatments had greater fresh mass and higher crown diameter than the plants in the 0 and 450 hour treatments. Finally, all cultivars except ‘Sensation’ had more flowers on plants in the 100 and 250 hour treatments than those of the 0 and 450 hour treatments.
Speakers
MP

Michael Palmer

North Carolina State University
Co-authors
MH

Mark Hoffmann

North Carolina State University
NA
Wednesday September 25, 2024 8:15am - 8:30am HST
Coral 2

8:15am HST

CITR 2/HIST - Cover Crop Effects on HLB-affected Citrus Tree Growth and Soil Characteristics: Results From a 3-year Field Trial
Wednesday September 25, 2024 8:15am - 8:30am HST
Citrus greening, or Huanglongbing (HLB), poses a severe threat to Florida’s citrus industry, impacting tree health and yield. Due to the lack of a known cure, growers employ various strategies to manage its effects, including increased nutrient application. However, in regions like the Indian River District (IRD), characterized by poor soil fertility and organic matter content, nutrient retention is challenging. Consequently, there is growing interest in using cover crops to enhance soil fertility. Despite this, there is limited published data on their effectiveness in the IRD. To address this gap, a collaborative study was initiated in Ft. Pierce, Florida, involving a commercial grower and the University of Florida. A three-year field trial, employing a randomized complete block design with four blocks, included ‘Star Ruby’ grapefruit trees grafted on ‘US-942’ rootstock, ‘Bearss’ lemon trees on ‘Sour Orange’ rootstock, and ‘OLL’ sweet orange trees on 'US-942' rootstock. Two treatments were applied: conventional (without cover crops) and experimental (with cover crops). Cover crops were planted bi-annually (winter and summer), grown, and terminated at each season’s end. Soil nutrient content, organic matter, microbiome diversity, moisture, and temperature, as well tree and root growth parameters were measured seasonally. After three years of cover cropping, significant differences in soil nutrient content, organic matter, and microbiome diversity were observed between the conventional and experimental treatments, suggesting potential impacts of cover crops on soil characteristics. However, there were no noticeable effects on tree growth or physiology. This study is ongoing and aims to provide a comprehensive understanding of the long-term effects of cover cropping on soil parameters in HLB-affected citrus groves, informing sustainable management practices in citrus cultivation.
Speakers
avatar for Lorenzo Rossi

Lorenzo Rossi

Associate Professor, University of Florida
Dr. Rossi’s research program focuses on improving root health and growth on cultivated crops, leading to the development of environmentally sound and effective management methods. He is a horticulturist with specific expertise related to plant stress physiology, root dynamics and... Read More →
Co-authors
AW

Alan Wright

University of Florida
NA
JF

John-Paul Fox

University of Florida
LH

Lukas Hallman

University of Florida
Wednesday September 25, 2024 8:15am - 8:30am HST
South Pacific 1

8:15am HST

FLOR 1 - Phosphate Solubilizing Bacteria Isolated From Greenhouse Ornamentals Solubilize Phosphate In Vitro And Ameliorate P Deficiency Symptoms In Marigold ‘Durango Yellow’ Fertilized With Calcium Phosphate.
Wednesday September 25, 2024 8:15am - 8:30am HST
Phosphorus (P) is an essential macronutrient absorbed by plants as orthophosphate (PO4). P availability depends on the pH of the substrate. At high pH, P forms insoluble compounds like Ca3(PO4)2 which is unavailable for plant uptake. Phosphate solubilizing bacteria (PSB) are plant-associated microorganisms that can break down Ca3(PO4)2 by secreting organic acids. PSB have been primarily evaluated as inoculum for crops grown in soil to improve P availability. However, less is known about the application of PSB in ornamentals grown in soilless substrates. Our goal was to identify PSB from a collection of bacteria isolated from the rhizosphere of greenhouse ornamentals. First, the collection was screened in vitro for the bacterial capacity to reduce pH of the media using the bromophenol-blue color assay. Thirty-five isolates were identified to reduce media pH, and their P solubilization capacity was quantified using ion chromatography. Fourteen isolates with the highest P solubilization were selected for whole-genome sequencing, but only two bacterial isolates (C2B11 and C8D10) were advanced to the in-planta evaluation using Marigold (Tagetes patula) 'Durango Yellow' grown in a peat-based substrate (pH = 7). Plants were irrigated with 100 mg·L-1 N from a 15N-0P-15K fertilizer, and P was supplemented weekly as Ca3(PO4)2 via substrate drench applications. Lalrise Vita (Lallemand Plant Care) and Bacillus velezensis (the active ingredient in Lalrise Vita) were included as positive controls. Plant phenotyping was conducted using the TraitFinder automatic system (Phenospex). TraitFinder assessment parameters included digital biomass, bloom area, Green Leaf Index (GLI), Normalized Pigment Chlorophyll Ratio Index (NPCI), and Plant Senescence Reflectance Index (PSRI). Lalrise Vita performed better than the control in all the parameters evaluated. B. velezensis increased bloom area and showed higher GLI and lower NPCI than the control. Pantoea sp. C2B11 significantly increased digital biomass, bloom area, and GLI. Pantoea sp. C8D10 only showed an increase in GLI values. Both C2B11 and C8D10 possess various genes involved in gluconic acid production. We identified two PSB that solubilize P in vitro, promote growth and bloom area, and improve canopy health (high GLI or low NPCI) in marigold plants fertilized with Ca3(PO4)2. Our next step is to validate the growth-promoting capacity of the identified PSB in other ornamentals. PSB can contribute to improving phosphorus nutrition and fertilizer use efficiency in greenhouse ornamental production.
Speakers
JQ

Juan Quijia Pillajo

The Ohio State University
Co-authors
MJ

Michelle Jones

The Ohio State University
NN

Nathan Nordstedt

FMC Corporation
NA
Wednesday September 25, 2024 8:15am - 8:30am HST
Nautilus

8:15am HST

GG 1 - Rediscovering Lost Heirloom Apple Cultivars with DNA Fingerprinting
Wednesday September 25, 2024 8:15am - 8:30am HST
U.S. heirloom apple cultivars are an underutilized and threatened resource, that DNA fingerprinting can help save and remobilize. These heirlooms are old cultivars that were named, clonally propagated, and distributed more than a century ago. Many heirloom cultivars have great historical value, some are still grown commercially, and others could be reintroduced to enhance rural prosperity and diversify options for consumers. While some heirloom cultivars are ancestors of modern cultivars, many others could be valuable for future breeding. However, most heirlooms have been long neglected, and thousands once documented are already extinct. Before more heirlooms disappear forever, mystery trees need to be distinguished from known cultivars, identified, and adequately preserved. Leaf samples for more than 2000 apple trees in collections, national heritage sites, old orchards, and backyards across the U.S. were crowdsourced from about 150 “MyFruitTree” submitters and DNA fingerprinted using KASP genotyping with 48 SNPs. The cultivar identity or uniqueness of each tree was determined by comparing obtained DNA profiles to a previously developed dataset of several thousand apple cultivars and individuals. Trees with replicates were prioritized into five categories according to several criteria for likelihood of representing heirloom cultivars. After removing duplicate samples, poor genotypic data, and non-apple samples, about 60% (1202) of samples were identified as cultivars and most were heirlooms. Of the unidentified samples, 85% (665) were unique, and 15% (118) of the samples represented replicated trees. We found five “Priority 1” trees (filled cultivar pedigree gaps or from three U.S. regions) and four “Priority 2” trees (detected in two regions). Hundreds more trees likely representing unknown heirlooms were also identified in single regions. Collaborators across the country, including historians and citizen scientists, can now closely examine the highest priority trees to uncover their historic cultivar names, while ensuring they are propagated so that they are preserved and valued once again. As more old apple trees are DNA fingerprinted, it is expected that current “unique” DNA profiles will be replicated in the same or other regions. Replicated trees must represent propagated, valued, and likely named cultivars, increasing the opportunities to rediscover lost heirlooms.
Speakers
DL

Dongyun Lee

Washington State University
Co-authors
AD

Amy Dunbar-Wallis

University of Colorado
NA
CP

Cameron Peace

Washington State University
JB

John Bunker

Maine Heritage Orchard
NA
RM

Rebecca McGee

USDA-ARS Pullman
NA
TL

Todd Little-Siebold

College of the Atlantic
NA
Wednesday September 25, 2024 8:15am - 8:30am HST
Coral 1

8:15am HST

PGR 1 - Effects of a Cytokinin-Containing Biostimulant Applied at Different Phenological Timings on Almond (Prunus dulcis) Yield
Wednesday September 25, 2024 8:15am - 8:30am HST
Almond (Prunus dulcis) is one of the most important crops in California. This commodity represented an economic impact of over 3.5 billion dollars for the state in 2022. During that year, 1,630,000 million planted acres were reported. In 2023, this number dropped by 74,000 acres, continuing the trend of decline due to numerous challenges that the industry has been facing. These include volatile prices, high input costs, reduced water allocations, climate unpredictability, and high temperatures during key phenological timings. The almond crop is highly sensitive to environmental factors, and photosynthetic rates can significantly decrease when temperatures reach 94F, heat levels are easily reached during the hot summer months in California’s production areas. To this matter, significant contributions are needed to support the industry. There are over 700 peer-reviewed scientific publications that evidence the benefits of using biologicals, which include plant biostimulants (PBS), in agriculture. It has been shown that season-long exogenous applications of cytokinin-containing PBS can support higher marketable yields. It is important to understand the physiological timings in which these applications have the most impact, in terms of maximizing the yield and quality potential. In 2023, a randomized complete-block design (RCBD) study was conducted on 5th leaf Var. Nonpareil in California’s Central Valley. The objective was to evaluate the yield and quality effects of an application of X-CyteTM, a 0.04% cytokinin-containing biostimulant registered for use on almonds in California, at different standalone phenological timings. These included early bloom, full bloom, petal fall, may spray, and hull-split. The trial consisted of six four-tree replications per treatment. Yield and quality data were subjected to a one-way ANOVA using IBM® SPSS® Statistics, and means were separated using Tukey’s HSD. The standard grower practice (SGP) produced 1801.5 marketable pounds of kernel meat per acre. X-Cyte™ treatment yields of 1845.16, 2140.16, 2215.66, 2137.67, and 2318.33, were observed for early bloom, full bloom, petal fall, may spray, and hull-split timings, respectively. Statistically significant differences were observed (p-value: 0.003). The hull-split and petal fall timing applications represented the higher yield increases (516.8 and 414.2 lbs/A, respectively) overall, compared to the SGP. It has been documented that taking place at these timings are key phenological stages deemed as major contributors to the components of yield: fruit set and nut fill. Significant differences in kernel size were not observed (p-value: 0.549). These findings further support and help fine-tune the use of biologicals and PBS in agricultural production systems.
Speakers
OT

Orlando Tapia

California State University Fresno / Corteva Agriscience
Co-authors
DG

Dave Goorahoo

California State University, Fresno
RM

Ryan Miller

Corteva Agriscience
NA
Wednesday September 25, 2024 8:15am - 8:30am HST
South Pacific 3

8:15am HST

PLAST - Influence of Plastic Mulch Color on Yield and Quality of Day-neutral Strawberries
Wednesday September 25, 2024 8:15am - 8:30am HST
High tunnel production of day-neutral strawberries is a promising production system in the central U.S. The use of different colored plastic mulches in a high tunnel production system can vary the microclimate around plants and alter yield and fruit quality. This study was conducted to identify the most appropriate color plastic mulch in a high tunnel production system as it relates to yield and fruit quality. The experiment was conducted at the Kansas State University, Olathe Horticulture Research and Extension Center in 2020 and 2021 using a split-plot, randomized complete block design. We evaluated six plastic mulches (black, white, striped silver, silver, red, and green) and two day-neutral cultivars, ‘Albion’ and ‘Portola’. Soil temperature and UV light reflected from the mulch were measured to monitor microclimate modifications. Fruit quality was assessed by overall visual quality, color, soluble solids content, titratable acidity, sugar/acid ratio, total phenolics, and anthocyanin content of strawberries. 'Portola’ produced greater yields than ‘Albion’, but ‘Albion’ had better fruit quality. The plastic mulches altered the reflected UV light and soil temperatures. During the mid and late season, the silver mulch had higher fruit yields than the red and green mulches, likely due to its ability to limit solar warming during warm production months. The mulches had inconsistent impacts on fruit quality. Overall, the silver mulch maintained the best microclimate for day-neutral strawberry production in high tunnels.
Speakers
avatar for Tricia Jenkins

Tricia Jenkins

Kansas State University
Co-authors
AM

Amrita Mukherjee

University of California, Davis
Amrita Mukherjee, Urban Agriculture/Small Farm Advisor at the University of California Agriculture and Natural Resources in Southern California, focuses on research and education in organic farming, urban agriculture, and sustainable practices for small-scale growers in both field... Read More →
CR

Cary Rivard

Kansas State University
EP

Eleni Pliakoni

Kansas State University
Wednesday September 25, 2024 8:15am - 8:30am HST
Lehua Suite

8:15am HST

QUAL - Bacteriophage as an Alternative Method to Control Salmonella enterica in Water-Recirculated Systems for Lettuce Production
Wednesday September 25, 2024 8:15am - 8:30am HST
In recent years, controlled environment agriculture (CEA) has gained popularity as a sustainable and efficient method of cultivation, offering solutions to challenges posed by traditional farming practices and meeting the growing demand for high-quality produce. However, advancements in CEA have raised concerns about food safety, requiring new approaches to minimize the risk of produce contamination. This research explores the effectiveness of a bacteriophage cocktail as a biocontrol agent against Salmonella contamination in lettuce grown in water-recirculating systems. Salmonella Newport and Salmonella Typhimurium (103 CFU/mL) were inoculated into aquaponic and hydroponic nutrient solutions into a water-recirculating system to mimic sporadic contamination, followed by treatment with a bacteriophage cocktail (S7, S10, and S13) at different multiplicities of infection (MOI 0.01 and MOI 1). The results demonstrated a significant reduction in Salmonella Newport and Salmonella Typhimurium populations at both MOI 0.01 and MOI 1 in aquaponic and hydroponic nutrient solutions, with levels reaching below the limit of detection (LOD) after 3 to 4 days of bacteriophage cocktail inoculation. From the plant parts, there was a significant reduction in the microbial population of Salmonella serovars in media plugs and roots from the hydroponic nutrient solution, reaching levels below the LOD in both phage cocktail treatments after a 2-day inoculation period. Contrarily, significant reductions were not observed in Salmonella serovar levels in plant roots and media plugs from the aquaponic nutrient solution treatment. These findings highlight the potential of utilizing bacteriophages to improve food safety in indoor-grown lettuce by controlling Salmonella populations while also indicating the necessity for further research to understand the microbial dynamics within each type of system.
Speakers
CR

Camila Rodrigues

Auburn University
Co-authors
CB

Caroline Blanchard

Auburn University
DW

Daniel Wells

Auburn University
NA
VM

Vania Mickos

Auburn University
NA
Wednesday September 25, 2024 8:15am - 8:30am HST
Kahili

8:15am HST

TECH 1 - Integrating UAV Imagery and AI to Forecast Vidalia Onion Yield and Quality
Wednesday September 25, 2024 8:15am - 8:30am HST
Forecasting yield and quality of Vidalia onions allows the stakeholders to make decisions on the best time and place to harvest. While yield defines an important quantitative parameter, conversely, sweetness emerges as timely factor of quality. Traditionally, measuring these parameters requires a field team and routine laboratory for the assessments, making it a subjective, time-consuming, labor-intensive, costly, and not-scalable approach. However, image technology and artificial intelligence (AI)-based methods could improve decision-making strategies. In this study, we collected unmanned aerial vehicle (UAV) multispectral images of two Vidalia onions fields from crop establishment until the harvest date, totaling six sets of images for each field. Each flight was performed with approximately 15 days apart. At the harvest date, 50 samples were collected in each field to determine yield, while 10 samples were used for sweetness. To ensure the robustness of the dataset, both fields were combined into a single dataset. Consequently, we used machine learning (ML) algorithms to perform predictive models, namely multiple linear regression (MLR), random forest (RF), and support vector machine (SVM). The dataset was split into 70% and 30% for training and testing, respectively, and the predictions were performed using the test dataset. Regarding the assessment of the models, we used the metrics namely coefficient of determination (R2), mean absolute error (MAE), and root mean squared error (RMSE). The models with higher R2 and lower MAE and RMSE were the bests. Notably, the considerable correlation between yield and spectral data made the MLR model perform well as more complex models such as RF. Conversely, when there was a weak correlation between the sweetness and spectral data, RF model could perform much better. In short, both models (MLR, RF, and SVM) could perform well into a predictive model, which highlights the strength of spectral data for representing Vidalia onions either quantitative or qualitative parameters. Therefore, our study not only represents an innovation in the field of specialty crop production, but also brings ready-to-use solutions to improve the production process and introduce Vidalia onions into the concept of field technology.
Speakers
avatar for Marcelo Barbosa

Marcelo Barbosa

University of Georgia
Co-authors
LO

Luan Oliveira

University of Georgia
NA
LS

Lucas Sales

University of Georgia
Agronomy Engineer graduated from the Federal University of Paraíba. With experience in the management and cultivation of Ornamental Plants, through a year of experience working in Greenhouses in the state of New Hampshire, USA. Experienced in the management and cultivation of vegetables... Read More →
RD

Regimar dos Santos

University of Georgia
Bachelor's degree in agronomic engineering from the Federal University of Mato Grosso do Sul, Brazil at 2021. Master's degree in plant production with an emphasis on computational intelligence in genetic improvement at 2022, with a doctorate in progress at the state university of... Read More →
Wednesday September 25, 2024 8:15am - 8:30am HST
South Pacific 2

8:15am HST

VCM 2 - Improving the Growth and Yield of Tomato through new Integrated Pest Management Strategies
Wednesday September 25, 2024 8:15am - 8:30am HST
Tomato (Solanum lycopersicum L.) is the number one vegetable crop in Hawaii in terms of popularity and market value. Of the total tomatoes consumed in Hawaii only 23% is produced locally. Local production has decreased substantially over the past few years due to crop losses caused primarily by the Tomato Yellow Leaf Curl Virus (TYLCV), which is vectored by whiteflies Bemisia tabaci and Bemisia argentifolii. Crop losses by TYLCV in tomato crops on Maui range between 60 to 100%. Efforts to keep plants from becoming infected, manage the rate of infection, timing, or severity of the infection are needed to protect crop health. Therefore, a new integrated pest management approach was conducted integrating the use of reflective ground cover, companion plants, insecticides, and tomato varieties resistant to TYLCV. The highest total weight (4.4 lb/pl per harvest time) was observed in the variety PS01522935 in the treatment combining reflective ground cover and companion plants and the highest marketable weight was observed in the same treatment in the varieties PS01522935 and Mesquite (3.5 lb/pl per harvest time), and the highest TYLCV infection was observed in the conventional treatment in the varieties Paisano followed by Healani, Celebrity Plus and Kewalo, and the varieties with no TYLCV infection were PS01522935, SVTD8601 and Mesquite. The use of reflective ground cover and companion plants may reduce TYLCV infections in tomato crops by reducing whitefly populations.
Speakers
RG

Rosemary Gutierrez Coarite

University of Hawaii at Manoa
Wednesday September 25, 2024 8:15am - 8:30am HST
South Pacific 4

8:30am HST

CE 2 - Utilizing Controlled Environment Agriculture to Enhance the Yield and Flavor of Strawberries
Wednesday September 25, 2024 8:30am - 8:45am HST
Presentation: Oral ASHS 2024 Keywords: CEA, greenhouse, Fragaria ×ananassa, temperature Utilizing Controlled Environment Agriculture to Enhance the Yield and Flavor of Strawberries Nicholas Cooley, Joshua Vanderweide, and Roberto Lopez In the U.S., strawberries (Fragaria ×ananassa) are the most popular berry fruit with a value of $2.8B. In 2022, strawberries experienced 12% growth in annual sales, with the majority of field production occurring in California and Florida. To meet consumer demand for flavorful, fresh, local, and year-round fresh strawberries, the industry is expanding controlled environment (CE) production of day-neutral (everbearing) cultivars in greenhouses and indoor farms. Within CEs, growers can potentially meet these demands through the manipulation of environmental parameters such as temperature, light, vapor pressure deficit, and carbon dioxide concentration. Despite the recent growth, CE growers are reporting low profitability. This imbalance of production and profitability stems from high energy costs, supra-optimal greenhouse temperatures during parts of the year, and low yield from the industry standard cultivar ‘Albion’. For producers to be considered profitable, they must reach an approximate annual yield of 15 kg∙m–2, which equates to a weekly yield of around 0.3 kg∙m–2. The objectives of our research are to 1) quantify the yield of other day-neutral cultivars in greenhouses; 2) determine how day and night temperature influence yield and fruit quality parameters; and 3) develop a model to predict the cardinal temperatures of each cultivar. Three cultivars, ‘Albion’, ‘Cabrillo’, and ‘Monterey’ were grown at day/ night temperatures (12 h/ 12 h) of 15/7, 18/10, 21/13, 24/16 or 27/19 °C, under a 16-h photoperiod, and a target DLI of 15 mol·m–2·d–1. Fruits were harvested three times weekly and at harvest, berry weight, diameter, color, shape, distortion, brix content, and flavor-related volatile organic concentrations were recorded. After 12 weeks of harvest, the highest average weekly yield was 0.17, 0.19, and 0.24 kg∙m–2 for ‘Albion’, ‘Cabrillo’ ‘Monterey’ at 18/10, 24/16, and 24/16 C, respectively. While the highest combined overall yield for all three cultivars was at 24/16 °C, the highest average berry weight differed. At day and night temperatures of 18/10, 18/10, and 15/7 °C, ‘Albion’, ‘Cabrillo’, and ‘Monterey had the highest average berry weights, respectively. Our results collectively indicate there are higher yielding day-neutral cultivars than the industry standard ‘Albion’.
Speakers
NC

Nicholas Cooley

Michigan State University
Wednesday September 25, 2024 8:30am - 8:45am HST
Coral 2

8:30am HST

CITR 2/HIST - More Frequent Irrigation Increases Yield in HLB-Affected Sweet Orange
Wednesday September 25, 2024 8:30am - 8:45am HST
Huanglongbing (HLB) causes a steady decline in tree health. Part of this decline includes root dieback which limits their capacity to take up water. For this reason, affected trees tend to be more susceptible to drought stress. This raises a significant concern during the dry season (Oct-May) when trees are largely dependent on supplemental irrigation for water. Unfortunately, most growers continue using irrigation schedules that were optimized for healthy trees. We hypothesized that irrigating more frequently, but in smaller doses would provide more opportunities for uptake and improve water relations in HLB-affected trees. The control treatment received the standard practice of irrigating every other day for 2 hours (12 gal/hr). The experimental treatment received water every day, 3 times a day, for 20 minutes (12 gal/hr). The two treatments received the same amount of water over the course of a week, but the experimental treatment received water more often. Treatments were initiated prior to flowering in January 2022 and were continued for two years. Tree water status improved in the experimental trees as reflected in higher mid-day leaf water potentials than in the control. This suggests the experimental regime was better able to maintain tree water status than the conventional method. In the second year, flowering was more synchronized in the experimental treatment. The control treatment saw two peaks in bud production with the latter one being consistent with a drought stress-induced flowering event. The experimental trees also saw an increase in fruit set in both years. Trees receiving the experimental and control treatments dropped a similar proportion of their crop load during June Drop and preharvest fruit drop in year 1 and 2. Finally, the experimental treatment resulted in significantly higher yields on average than the control in both year 1 and year 2 (72% and 200%, respectively). Altogether, more frequent irrigation improved tree productivity.
Speakers
TV

Tripti Vashisth

University of Florida
Co-authors
MS

Mary Sutton

University of Florida
NA
Wednesday September 25, 2024 8:30am - 8:45am HST
South Pacific 1

8:30am HST

FLOR 1 - Activated Aluminum Amended Substrates Reduce Phosphorus Leaching in Floriculture Production
Wednesday September 25, 2024 8:30am - 8:45am HST
The use of water-soluble fertilizers in floricultural production provides readily available nutrients to sustain short-term crop production; however, nutrients such as phosphorus are poorly retained in typical peat substrates. Activated aluminum is an amendment that has demonstrated success in binding phosphorus within substrates, reducing the amount of phosphorus that is leached from the container. This research investigated the production of Tagetes in peat-based substrates amended with or without activated aluminum and provided four different phosphorus fertilizer regimens. The fertilizer regimens encompassed a nitrogen, phosphorus, and potassium liquid blend applied weekly; however, phosphorus was only included for 0, 2, 4, or 6 weeks in total over the duration of the study. Growth of Tagetes was effectively similar, and leachate analysis provided insights that can be applied towards more efficient production methods. In this research, utilizing activated aluminum resulted in less phosphorus loss in container leachate than unamended substrates. The potential to decrease applied phosphorus during floricultural production can similarly be achieved when using substrates amended with activated aluminum. This presentation will decipher the dynamics of the movement of phosphorus and other anions of interest that may be relevant to sustainable floriculture production.
Speakers
DA

Damon Abdi

Louisiana State University Agricultural Center
Co-authors
JF

Jeb Fields

LSU AgCenter Hammond Research Station
JB

Jeffrey Beasley

University of North Carolina - Pembroke
NA
Wednesday September 25, 2024 8:30am - 8:45am HST
Nautilus

8:30am HST

GG 1 - Air or Soil Temperature: Understanding the Cues for Dormancy Transition in Peach
Wednesday September 25, 2024 8:30am - 8:45am HST
Peach trees require quantitative exposure to winter chilling (chilling requirement, CR) for spring bloom. The chill accumulation time points are determined using weather data of air temperatures between 32 and 45 ºF, using various calculation methods such as the simple chill hour (CH) method or more sophisticated methods like Utah and Chill portions (CP) that account for negations of chilling due to warm weather during the dormancy. All these methods rely on air temperature and do not consider the soil temperature during the dormancy and its effect on the tree’s perception and account for chill accumulation. Peach flowers and developing fruit are highly sensitive to freezing temperatures and are killed following even a limited exposure. In the past decade, mild winters and early spring frosts have significantly reduced or eliminated the annual peach production in the southeast U.S. Low-chill winters have become increasingly common in the southeastern peach-producing regions, and when followed by warm springs, result in early bud break and early flowering, increasing the risk of crop loss to frost. Due to a replant issue caused by Armillaria root rot, almost all acreage under the new peach orchards in the southeast, including South Carolina, are planted on berms adopting root collar excavation as a method to extend the life of orchards on infested soil. We observed significant differences between the air temperature and temperature of undisrupted soil and soil within berms at various depths (3, 6, 12 and 18 in) during dormancy. The effect of observed temperature differences on ‘Cresthaven’ tree chill accumulation calculation and transition between endo- and eco-dormancy stages was investigated by collecting vegetative bud and root tissue from all four depths at six chill hour time points (400, 500, 600, 700, 800 and 900). Preliminary data show significant gene expression differences between bud and root tissue and different gene expression profiles related to the chill accumulation in each tissue. Detailed analyses of the gene expression profiles between the tissues at the different chill accumulation stages and their effect on chilling and heat accumulation, bloom time, and the transition between the dormancy stages in peaches will be discussed.
Speakers
avatar for Ksenija Gasic

Ksenija Gasic

Clemson University
Co-authors
CS

Christopher Saski

Clemson University
NA
JL

John Lawton

Clemson University
NA
SP

Stephen Parris

Clemson University
ZL

Zhigang Li

Clemson University
NA
Wednesday September 25, 2024 8:30am - 8:45am HST
Coral 1

8:30am HST

PGR 1 - The Application of a Cytokinin B-Mo-based Product Influences the Source-to-Sink Dynamics and Non-Structural Carbohydrate
Wednesday September 25, 2024 8:30am - 8:45am HST
Understanding the sink-to-source relationship on leafy crops offers valuable insights into optimizing resource allocation for enhanced plant growth and quality. Variations in growth rates and carbon pools across individual leaves or groups of leaves at similar developmental stages allow us to understand the plant strategies of carbon allocation and partitioning. We hypothesized that products that enhance the carbon source-to-sink relationship during leaf development can lead to increased growth and dry matter accumulation. This project aimed to determine if the exogenous application of a cytokinin and B-Mo-based product during leaf development would impact carbon source-to-sink relationship and, hence, influence plant growth and quality. The experiment was a complete randomized design with two treatments consisting of a negative control and the application of the product twice during the growing cycle. The experimental unit consisted of a deep-water culture reservoir with three lettuce plants. Destructive sampling was conducted at five sampling points. At each sampling point (n=4 per experimental run), the phenological stage was determined, and root and shoot length and dry matter, leaf length, width, area, and non-structural carbon and chlorophyll contents were measured. This data was used to estimate growth rates. Results indicate that the cytokinin and B-Mo-based product increased the number of true unfolded leaves by 1 ± 0.4 and the overall size of the lettuce head by 9%. The treated lettuce reached a marketable size four days earlier than the control treatment. Statistically significant differences were observed in the shoot and root dry matter accumulation and foliar length and width at some sampling points. Some of the growth indices indicate an increase in leaf surface area investment and enhanced conversion efficiency of assimilates into biomass in plants treated with the product. Plants exhibiting these alterations had higher sucrose and total soluble sugar content. There was a noticeable pattern of higher concentrations of non-structural carbohydrates, proteins, and amino acids in the leaves compared with the roots across all plants and treatments. Overall, our study on using a cytokinin and B-Mo-based product to strengthen the source-to-sink relationship during the development of a leafy crop provides new insights into non-structural carbohydrate metabolism and the role of CKs, B, and Mo in generating a high-quality plant in a shorter timeframe.
Speakers
avatar for Mayra Toro Herrera

Mayra Toro Herrera

Postdoctoral Research Associate, University of Connecticut
Co-authors
RR

Rosa Raudales

University of Connecticut
Wednesday September 25, 2024 8:30am - 8:45am HST
South Pacific 3

8:30am HST

PLAST - Exploring sustainable mulch solutions: a comparative study in strawberry production systems in northwestern Washington
Wednesday September 25, 2024 8:30am - 8:45am HST
Agricultural plastic mulch made from non-biodegradable polyethylene (i.e., “PE mulch”) provides many horticultural benefits. However, PE mulch requires annual removal and disposal, which generates large volumes of plastic waste that is rarely recycled and can become a pollutant. Incomplete removal of PE mulch also can leave behind plastic fragments that threaten soil and ecosystem health. To address these challenges, soil-biodegradable mulches (BDMs) have emerged as a potentially more eco-friendly alternative. BDMs are designed to provide the same advantages for specialty crop production as PE mulch and naturally biodegrade when incorporated into the soil. In addition, non-biodegradable reflective mulch has emerged as a promising mulch technology that may reduce key insect pests. The objective of this research was to evaluate the impacts of different mulch technologies, including soil-biodegradable and reflective mulches, on horticultural and pest dynamics in day-neutral strawberry (Fragaria×ananassa cv. Albion) in northwest Washington. Seven mulch treatments were established in a randomized complete block design with four replications in 2023. Treatments included green and black BDM made with ecovio and Mater-Bi feedstocks, non-biodegradable metalized mulch, and controls of black PE mulch and unmulched plots. Yield from all mulch treatments did not differ significantly from black PE control except for the green BDM made with ecovio feedstock. The green BDM made with ecovio resulted in significantly lower (32%) yield, similar to unmulched control. Lower yield may be attributed to the rapid deterioration of the green BDM treatments, which lacks carbon black and deteriorated more rapidly compared to other mulch treatments. However, black BDM made from MaterBi feedstock and metalized mulch were 37% and 11% less deteriorated than PE control respectively. Additionally, all mulch treatments suppressed weeds better than the unmulched control and were not different from black PE mulch. Aphid and thrips populations were highest in unmulched plots, whereas metalized mulch had fewer thrips (on sticky cards) and fewer aphids (on leaves) than all other treatments. Overall, the study highlighted that black BDM performs similarly to PE mulch with regards to potential weed suppression and maintenance of yield, whereas the rapid deterioration of green BDM could effect the soil microclimate and subsequent plant growth. Despite rapid mulch deterioration, the study did not observe compromised weed management. Metalized mulch may contribute to reducing pest populations but impacts on pollination and biological control remains unknown. Additional research on soil health effects is required given the fate of BDMs is in-soil incorporation.
Speakers
NG

Nayab Gull

Graduate Research Assistant, Washington State University
Wednesday September 25, 2024 8:30am - 8:45am HST
Lehua Suite

8:30am HST

QUAL - Transfer of Escherichia coli from Plastic Mulch to Tomato and Pepper Fruit by Ground Contact in a Field Environment
Wednesday September 25, 2024 8:30am - 8:45am HST
In the southeast US, tomatoes (Solanum lycopersicum) and bell pepper (Capsicum annuum) are typically staked and trellised and grown using plastic mulch for weed control. The Produce Safety Rule that is part of the Food Safety Modernization Act requires that fruit from tomato and pepper be declared non-harvestable if they fall to the ground or contact the ground through drooping while attached to the plant before harvest. The objective of this study was to quantify percent transfer from plastic mulch inoculated with Escherichia coli with green fluorescent protein (GFP) to pepper and tomato fruit making ground contact in spring and fall growing seasons. E. coli GFP was spot inoculated on plastic mulch in the field using ten - 10 µL drops (100 uL total) at approximately 7 log CFU/mL and allowed to dry for at least 1 h before fruit contact. In the spring season, white and black colored plastic mulch were compared and in the fall season, reused (second crop) plastic mulch and new white plastic mulch were compared. Fruit contacted the ground by drooping (remained attached to the plant) with a contact time of 1 h or 24 h as well as contact through dropping fruit at heights of 30 cm, 60 cm, and 120 cm (n=9 per treatment). After inoculum was allowed to dry on the plastic mulch, the E. coli population was reduced an average of 2.8 and 4.8 log CFU/mL, in the spring and fall seasons, respectively. In both seasons all dropped peppers had significantly different percent transfer than dropped tomatoes, while drooping fruit were not significantly different between the two commodities. In the fall, dropped fruit on new plastic had significantly greater bacterial transfer than fruit which was dropped on reused plastic for both tomato and pepper. No significant differences were obtained between drooping duration treatments or between the different height of dropping in either season. There was relatively low transfer overall from both drooping and dropping of fruit on inoculated plastic mulch, regardless of crop or season. Further, in this inoculated study, results suggest that there was a significant reduction in E. coli populations in a field setting after a short period of exposure to the natural environment.
Speakers
AB

Autumn Burnett

University of Georgia
Co-authors
BR

Blanca Ruiz Llacsuanga

University of Georgia
NA
FC

Faith Critzer

University of Georgia
NA
HG

Halle Greenbaum

University of Georgia
NA
RR

Rawane Raad

University of Georgia
NA
TC

Timothy Coolong

University of Georgia
Wednesday September 25, 2024 8:30am - 8:45am HST
Kahili

8:30am HST

TECH 1 - Deep Learning Application for Field Phenotyping of Shoot Structure in Grapevine
Wednesday September 25, 2024 8:30am - 8:45am HST
In the cultivation of fruit trees and vines, plant architecture is a critical determinant of productivity. While there are considerable diversities in plant architecture, which can be modified through pruning in fruit production, a method for high-throughput measurement and recording of architecture has not yet been established, posing a limitation to research and development in this area. Here we evaluated Transformer-based architecture for detecting above-ground shoot network of grapevine in an outdoor vineyard condition. The problem here was defined as the detection of nodes (buds or branching points) and their physical relationships (internodes or edges) within plant images. We also developed an evaluation metric inspired by the inherent structure of plant shoots to efficiently smooth detected structures to more closely resemble realistic systems in plants. The proposed framework has been successfully applied to the detection task in outdoor condition with complex background. Through the application of this method, we have demonstrated that our proposed framework is capable of extracting topological parameters of dormant shoot architecture of grapevine that effectively models the shoot biomass in a large-scale vineyard.
Speakers
SN

Soichiro Nishiyama

Kyoto University
Co-authors
DG

Dario Guevara

Department of Viticulture
NA
GG

Guillermo Garcia Zamora

Department of Viticulture
NA
ME

Mason Earles

Department of Viticulture
NA
Wednesday September 25, 2024 8:30am - 8:45am HST
South Pacific 2

8:30am HST

VCM 2 - Assessment of Novel, Interspecific Hybrid Tomato Rootstocks for Production under Environmental Stress
Wednesday September 25, 2024 8:30am - 8:45am HST
A growing global population and worsening global environmental change necessitate the development of improved crop varieties and cultivation techniques. In tomatoes, the world’s most popular vegetable crop, grafting has emerged as a cultivation technique for addressing these challenges through improving tomato crop vigor and stress resistance, especially to abiotic stresses that are becoming more widespread including heat, salinity, and drought stress. In order to expand the offerings of tomato rootstocks, we have conducted multiple trials to assess the beneficial impact of 10 novel tomato rootstocks on ‘Celebrity’ scion vigor and stress resistance. The rootstocks, developed by colleagues at the King Abdullah University of Science and Technology (KAUST) consist of interspecific Solanum spp. hybrids including two tetraploid hybrids. We conducted three trials to assess the impact of grafting with the novel rootstocks, including a greenhouse pot trial to quantify growth and vigor and two controlled environment trials to assess stress resistance. In each trial, ‘Celebrity ’ scions were grafted with either the novel rootstocks, ‘Maxifort’, ‘Multifort’, or left ungrafted. In the greenhouse trial, measurements of stem diameter, shoot height, shoot canopy projection area, and fresh and dry weights at harvest were used to determine overall vigor. We identified five and eight of the novel rootstock grafting combinations that outperformed the ungrafted and ‘Maxifort’-grafted plants, respectively. Additionally, measurements of SPAD and observations of foliage coloration provided initial evidence of differential nutritive requirements between the novel rootstocks. In our controlled environment trials, chlorophyll fluorescence, SPAD, and porometer data demonstrated differential susceptibility to salinity, heat, and drought stress between the rootstocks. This presentation will highlight key differences in morphological and physiological traits that assisted us in selecting a narrow set of rootstocks for a forthcoming evaluation in a passive high tunnel to demonstrate the utility of these new rootstocks.
Speakers Co-authors
NR

Nathaly Rodriguez Ortiz

King Abdullah University of Science and Technology
NA
Wednesday September 25, 2024 8:30am - 8:45am HST
South Pacific 4

8:45am HST

CE 2 - Impact Of Elevated CO2 And Two Daily Light Integrals on the Production Efficiency of Strawberry (Fragaria × ananassa ‘Monterey’) Daughter Plants
Wednesday September 25, 2024 8:45am - 9:00am HST
Abstract: Strawberry nurseries face many challenges, and are considering controlled environment propagation as an alternative to conventional open-field propagation. Limiting factors to economic feasibility include stock plant yield (number of daughters produced per stock plant). From published research we know that increasing CO2 concentration and light intensity increases strawberry photosynthetic rate, however there has been no research on the effects of these treatments on the total stock plant yield of daughters. Our hypothesis is that higher light intensity and CO2 concentrations will improve plant growth and lead to greater total daughter plant production. The objective of this experiment is to increase stock plant yield by increasing CO2 concentration (500, 850, and 1200 μmol mol-1) and light intensity (DLI 14.4 and 28.8 mol m-2 d-1). Strawberry (Fragaria × ananassa Duch., ‘Monterey’) stock plants were transplanted into three controlled environment growth chambers, under combinations of CO2 and DLI treatments in a split plot design under 26°C, 65% relative humidity, and a 16-hour photoperiod. The stock plants were grown under treatment conditions for 70 days, and newly-formed daughters were logged every day. At the end of the experiment the stock plants and their daughter plants were harvested, and each daughter plant was evaluated based on its size (number of leaves, leaf area, and fresh/dry mass). Increasing CO2 concentration linearly increased stock plant yield, leading to 23.96% more daughter plants per mother plant from the 500 to the 1200 μmol mol-1 treatment. Plants under higher light intensity (500 μmol m-2 s-1) had 38% higher stock plant yield than those under 250 μmol m-2 s-1. These data support our hypothesis that increasing CO2 concentration and light intensity increase the total yield of daughter plants produced per stock plant. By optimizing CO2 concentration and light intensity, strawberry nurseries may be able to grow more daughter plants more efficiently in controlled environment nurseries than in the conventional open-field system.
Speakers
SH

Samson Humphrey

Univ. of Tennessee
N/A
Co-authors
RH

Ricardo Hernandez

North Carolina State University
NA
Wednesday September 25, 2024 8:45am - 9:00am HST
Coral 2

8:45am HST

CITR 2/HIST - Impact of Different Organic Matter Contents on ‘US-942’ Citrus Rootstock Physiology, Nutrient Uptake, and Root Morpholog
Wednesday September 25, 2024 8:45am - 9:00am HST
The disease citrus greening (HLB, Huanglongbing) continues to decimate Florida’s citrus industry, resulting in the lowest yields since the 1940s. With no cure or tolerant rootstock/scion combinations available, growers must manage the disease. Although increased fertilizer applications have been shown to improve tree health, the additional inputs are expensive and decrease operational profitability. As a result, interest has grown in improving soil fertility parameters such as organic matter, with the goal of reducing inputs and increasing horticultural sustainability. Despite the recognized potential of organic matter, little research has been undertaken to establish the optimal contents required to improve citrus root characteristics. The objective of this study was to answer two specific questions: what are the amounts of organic matter needed to significantly affect citrus root growth and physiology? And how does the incorporation of organic matter influence nutrient availability? To answer these questions, a six-month completely randomized greenhouse experiment was conducted to measure the impact of different soil organic matter contents on potted citrus trees. Six different treatments consisting of different organic matter contents (0% - control, 1%, 2%, 3%, 5%, and >10%) were created by mixing different amounts of locally sourced compost with sand. One-year-old ‘US-942’ (Citrus reticulata x Poncirus trifoliata) rootstock plants (n = 6 per treatment) were grown in the different mixtures for six months under controlled greenhouse conditions. During the study, soil moisture, plant height, and stem diameters were assessed every other month. At the conclusion of the study, total plant and root biomass, root nutrients, average root length, and average root diameter were measured. Results from the study indicated that higher organic matter contents (>2%) led to significantly increased plant biomass and stem diameter, and increased root growth. Significant results were also found at the soil level, where increased organic matter contents led to increased nutrient retention and increased root uptake of nutrients. Additional investigation is needed to better understand the advantages of increasing organic matter content, even by marginal percentages, utilizing grafted citrus trees of various cultivars in field trials. Such trials would provide insights into the practical implications of these findings within the industry.
Speakers
LH

Lukas Hallman

University of Florida
Co-authors
AW

Alan Wright

University of Florida
NA
JF

John-Paul Fox

University of Florida
LR

Lorenzo Rossi

University of Florida
Wednesday September 25, 2024 8:45am - 9:00am HST
South Pacific 1

8:45am HST

FLOR 1 - Iron-coated Sand as a Sustainable Substrate Amendment for Nutrient Management of Containerized Floriculture Crops
Wednesday September 25, 2024 8:45am - 9:00am HST
As natural resources dwindle, sustainable alternatives to current fertilization methods are essential for environmental and economic progress. Acid mine drainage (AMD) and phosphorus from fertilizer runoff are significant sources of water pollution in the Appalachian region of the United States. Horticultural producers are faced with rising prices of phosphate fertilizers, putting the industry in a tenuous position: reducing these costly inputs to minimize water pollution but, in turn, diminishing product quality. AMD-based iron-coated sand is a novel phosphate sorbent that can potentially ameliorate AMD and prevent further phosphate pollution. This technology can cut growers’ input costs and slow the consumption of finite phosphate resources. This study aimed to determine the viability of iron-coated sand as a substrate amendment for reducing phosphate leaching and enhancing growth, flowering, and phosphorus uptake of floriculture crops during and after production. The ideal sand-to-potting mix ratio was determined based on pansies, petunias, and chrysanthemums' growth, flowering, and leachate content. The rate of applied phosphate and P-saturation of sand were determined from growth, flowering, and leachate data of chrysanthemum during production. Finally, the effects of the coated sand were examined on petunia and chrysanthemum growth, flowering, mineral content, and leachate composition over time in production and post-production environments. Twenty percent P-saturated iron-coated sand with low to moderate rates of applied phosphorus reduces leached phosphate with no deleterious, and usually positive, effects on the performance of floriculture crops during and after production. Iron-coated sand as a substrate amendment in container production of ornamentals has tremendous potential for advancing environmental and economic sustainability in the horticultural industry.
Speakers
SM

Savannah Mead

West Virginia University
Co-authors
EP

Eugenia Pena Yewtukhiw

West Virginia University
NA
KB

Karen Buzby

West Virginia University
NA
LL

Lance Lin

West Virginia University
NA
NW

Nicole Waterland

West Virginia University
NA
Wednesday September 25, 2024 8:45am - 9:00am HST
Nautilus

8:45am HST

GG 1 - Selection and Evaluation of Citrus Resistobiome for HLB Resistance/Tolerance
Wednesday September 25, 2024 8:45am - 9:00am HST
Since no Huanglongbing (HLB)-resistant citrus cultivar is available in the world, selection of elite natural mutants of commercial citrus for HLB-resistance/tolerance becomes a much more appealing breeding approach, especially in HLB-epidemic regions. In this study, we have selected and evaluated more than 30 citrus mutants from commercial citrus varieties in the past eight years in Florida. After greenhouse and field trials with high HLB disease pressure, we have identified several citrus lines with improved HLB-resistance/tolerance, which can be released or used for large scale of field trials. Our analyses of these lines have revealed that citrus resistobiome plays a role in the HLB resistance/tolerance, which involves a plant virus that can enhance plant resistance and illustrated the pursuit of breeding for biocontrol and a healthy microbiome. Meanwhile, we revealed that transposons have driven the selection and diversification of sweet orange (SWO). We identified six transposon families with up to 8900-fold activity increases in modern sweet orange cultivars tracing back to a common ancestor ~500 years ago. Notably, these six families of transposons contribute significantly to the formation of major cultivar groups, with frequent independent activations or accelerations observed in the breeding history of SWO. We will discuss the molecular mechanisms underlying the improved HLB-resistance, especially how the resistobiome plays a role in the improved HLB resistance/tolerance, and how to implement this new approach by utilizing and expanding the breeding of citrus resistobiome for the control of citrus HLB.
Speakers
YD

Yongping Duan

USDA ARS
NA
Co-authors
BW

Bo Wu

Clemson University
NA
DZ

Desen Zheng

USDA ARS
NA
FL

Feng Luo

Clemson University
NA
ZD

Zhanao Deng

University of Florida
Wednesday September 25, 2024 8:45am - 9:00am HST
Coral 1

8:45am HST

PGR 1 - PGR applications to reduce HLB-associated preharvest fruit drop in Sweet Orange
Wednesday September 25, 2024 8:45am - 9:00am HST
The Florida citrus industry has seen a steady decline in production since the arrival of Huanglongbing (HLB), or citrus greening disease, in Southern Florida in 2005. Following infection, trees experience a steady decline in health and productivity. HLB has since spread throughout all of Florida’s citrus producing regions resulting in nearly 100% infection rates in traditional field settings. Lamentably, no cure has been found for HLB, so research efforts have focused on mitigating the symptoms associated with this disease. Among the myriad of symptoms associated with HLB, the increased rates of mature fruit (preharvest fruit drop) is a major concern for growers. Not only do more fruit drop in HLB-affected trees, but fruit also begin dropping earlier in affected trees as well. This loss of mature, and potentially marketable, fruit in the months leading up to harvest represents a visual loss of revenue for the growers. The authors have previously reported that the likelihood of a fruit to drop during preharvest fruit drop is related to the size of that fruit; the relatively smaller fruit on the tree are more likely to drop during the preharvest fruit drop window. As plant growth regulators (PGRs) have had promising results in preventing fruit drop in many crop species, the efficacy of PGR applications in reducing preharvest fruit drop was evaluated. Thirty small and thirty large fruit were tagged on four 12-year-old ‘Valencia’ on ‘Swingle’ rootstock trees. Small fruit were those that were a ½ standard deviation below the average fruit size for that tree whereas large fruit were those that were a ½ standard deviation above the average. Ten of the small tagged fruit and ten of the large tagged fruit were then dipped into ProGibb® (33 ppm Gibberellic Acid [GA]), Citrus Fix® (106 ppm 2,4-Dichlorophenoxyacetic Acid [2,4-D]), or left untreated (control). Citrus Fix® improved retention in both small and large fruit whereas ProGibb® only improved retention in the large fruit. As GA prevents drop by delaying the senescence process, the lack of effect in the small fruit may suggest that they have already entered the senescence process. To further explore why the small and large fruit differ in their likelihood to drop and why they respond differently to PGR applications, additional biochemical and molecular analyses are underway.
Speakers
TV

Tripti Vashisth

University of Florida
Co-authors
MP

Maricielo Postillos Flores

University of Florida
NA
MS

Mary Sutton

University of Florida
NA
Wednesday September 25, 2024 8:45am - 9:00am HST
South Pacific 3

8:45am HST

PLAST - Shade level effects on fruit yield of habanero chili (Capsicum chinense Jacq.)
Wednesday September 25, 2024 8:45am - 9:00am HST
Habanero chili fruit is shiny, orange, or red, about 5 cm long, and very pungent (200,000 to 300,000 Scoville heat units). It is popular in Mexico, Central America, and the Caribbean, although it is increasing in demand in the U.S. There is reduced information on chili production under shade nets. The objective was to determine the effects of shade level and cultivar on the fruit yield of habanero chili. The study was conducted in Tifton, Georgia, under field conditions in two seasons. Habanero plants were grown using raised beds, black plastic film mulch, and drip irrigation, following the University of Georgia extension recommendations for bell peppers. The experimental design was a randomized complete block with four replications and 10 treatments (5 shade levels x 2 cultivar combinations). Habanero chili ‘Kabal’ and ‘Kukulkan’ were grown at 0% (unshaded), 30%, 47%, 62%, and 80% shade levels. Marketable fruit number and yield decreased quadratically with shade level, although there were no statistical differences in marketable yield for plants in unshaded conditions and 30% and 47% shade levels. Mean marketable yields were 33.6 t/ha (unshaded) and 5.7 t/ha (80% shade level). Less than 2.5% of total fruit were affected by sunscald and blossom-end rot. Total and marketable fruit numbers and yields and individual fruit size were higher in ‘Kukulkan’ than in ‘Kabal.’ In conclusion, although shading increased fruit size, it did not improve marketable yield compared to unshaded conditions.
Speakers
avatar for Juan Carlos Diaz Perez

Juan Carlos Diaz Perez

Professor of Horticulture, University of Georgia
Co-authors
MB

Mamata Bashyal

University of Massachussets
YK

Yamin Kabir

Khulna University
NA
Wednesday September 25, 2024 8:45am - 9:00am HST
Lehua Suite

8:45am HST

QUAL - Assessing the Impact of Biological Soil Amendments of Animal Origin on Bell Pepper Microbial Safety, Soil Properties, and Quality
Wednesday September 25, 2024 8:45am - 9:00am HST
The shift towards sustainable food production has forced farmers to explore alternative practices, such as the use of Biological Soil Amendments of Animal Origin (BSAAOs). However, concerns due to food safety regulations restrict the use of BSAAOs on crops consumed raw. This study assessed the microbial safety of bell peppers, the impact of BSAAOs on soil properties, and on quality. The field experiment was established with 36 plots (88 ft2) planted with bell pepper seedlings on 12-inch centers for a total of 20 plants/plot. BSAAO treatments consisted of either raw CM, PL, or non-BSAAO (NB) application, with each treatment being either tilled or top dressed. E. coli/Total Coliforms were determined from soil samples collected during weeks 1, 3, 6, and 12 and from harvested bell peppers during weeks 13 and 14. Nalidixic acid resistant E. coli was inoculated on bell peppers for a 5-day die-off determination (40 inoculated on the upper portion and 40 on the side). Ammonium and nitrate content in soil was determined before and after the establishment of the experiment, while SOM content was determined from soil samples collected after the experiment. Higher levels of E. coli (1.1–2.6 log CFU/g) and total coliforms (4.1–4.7 log CFU/g) were reported in plots with BSAAO application as compared to NB plots (1.00–1.17 log CFU/g; 3.89–4.14 respectively), although levels decreased significantly after one week. E. coli levels on bell peppers previously inoculated declined by over 2 logs within four days, with a die-off rate of 0.57 and 0.51 log CFU/cm2/day for bell peppers inoculated on top and on the side, respectively. CM tilled plots had slightly higher E. coli levels (0.02 log CFU/ cm2). The quality of bell peppers was unaffected by BSAAOs. NB top dressed and CM tilled plots had higher soil organic matter (SOM) content (2.18 and 2.08%, respectively) while both CM and PL increased soil nitrate and ammonium content significantly by the end of the experiment. CM and PL can enrich soil with inorganic nitrogen without compromising produce quality. However, the use of untreated BSAAOs poses a risk of cross-contamination, which highlights the importance of minimizing potential contamination events. Overall, this study highlighted the potential of CM and PL as BSAAOs to enhance soil fertility while maintaining food safety. It emphasized the need for appropriate management practices to mitigate risks associated with microbial contamination, ensuring the safety and sustainability of food production systems.
Speakers
DL

Daniel Leiva

Research Assistant, Louisiana State University
Co-authors
AA

Achyut Adhikari

Louisiana State University AgCenter
NA
JM

Juan Moreira

University of Arkansas
NA
KF

Kathryn Fontenot

LSU AgCenter
Wednesday September 25, 2024 8:45am - 9:00am HST
Kahili

8:45am HST

TECH 1 - Investigation of Using Hyperspectral Vegetation Indices to Assess Brassica Downy Mildew
Wednesday September 25, 2024 8:45am - 9:00am HST
Downy mildew, caused by Hyaloperonospora parasitica, poses a significant threat to Brassica oleracea crops, leading to substantial reductions in yield and marketability. This study seeks to assess various vegetation indices for detecting different levels of downy mildew infection in a Brassica variety, Mildis, using hyperspectral data. Through artificial inoculation with H. parasitica sporangia suspension, distinct levels of downy mildew disease were induced. Spectral measurements, ranging from 350 nm to 1050 nm, were performed on the leaves under controlled environmental conditions, and reflectance data were collected and processed. The Successive Projections Algorithm (SPA) and signal sensitivity calculations were employed to identify the most informative wavelengths, which were then used to develop Downy Mildew Indices (DMI). A total of 37 existing vegetation indices and three proposed DMIs were evaluated to assess downy mildew infection levels. The results revealed that a support vector machine achieved accuracies of 71.3%, 80.7%, and 85.3% in distinguishing healthy leaves from those with early (DM1), progressed (DM2), and severe (DM3) infections, respectively, using the proposed downy mildew index. The development of this novel downy mildew index has the potential to facilitate the creation of an automated monitoring system for downy mildew and aid in resistance profiling in Brassica breeding lines.
Speakers
BL

Bo Liu

Professor, California Polytechnic State University
NA
Co-authors
MF

Marco Fernandez

California Polytechnic State University
NA
SD

Shunping Ding

California Polytechnic State University
TL

Taryn Liu

California Polytechnic State University
NA
Wednesday September 25, 2024 8:45am - 9:00am HST
South Pacific 2

8:45am HST

VCM 2 - Evaluation of Biostimulant on Nutrient Use Efficiency in Tomato
Wednesday September 25, 2024 8:45am - 9:00am HST
To evaluate the efficacy of a commercial available biostimulant (a product containing friendly microorganisms like N2 fixing bacteria) on the improvement of nitrogen (N) and phosphorus (P) use efficiency in tomato, two independent field experiments were conducted in West Central Florida in the fall production season of year 2023. For both experiments, treatments included a factorial combination of two biostimulant rates (0 or 0.247 kg/ha) and four fertilizer rates (N or P2O5) (0, 67, 135, 202 or 269 kg/ha) that were arranged in a split-plot design. Biostimulant was applied as a solution drenched in soil following transplanting, and fertilizer treatments were applied as pre-plant application in soil and/or through drip injection during the season. Commercial tomato cultivar ‘HM1823’ was chosen as plant material. Analysis of variance (ANOVA) revealed that biostimulant did not have statistically significant influence on fruit marketable yield, fruit quality (Brix) and plant biomass, regardless of application rates of P2O5. By contrast, however, application of N had statistically (P
Speakers
WW

Weining Wang

University of Florida
Co-authors
LS

Lakesh Sharma

University of Florida
Dr. Lakesh Sharma is an assistant professor of soil fertility and sustainable agriculture at the University of Florida in Gainesville, FL. Lakesh has been farming since he was a child on his own farm. His academic school journey started in 2000. He is currently working on nutrient... Read More →
SA

Shinsuke Agehara

University of Florida
Wednesday September 25, 2024 8:45am - 9:00am HST
South Pacific 4

9:00am HST

CE 2 - Unraveling Strawberry Stock Plant Architecture, Morphology, and Tips’ Spatial Distribution under Three Photoperiods to Facilitate Future Propagation System Design: A Comparative Study on Monterey as a Long-day Cultivar
Wednesday September 25, 2024 9:00am - 9:15am HST
The US strawberry industry needs healthy, high-quality transplants every year for fruit production. Following the challenges in open-field nurseries, research is focused on controlled-environment agriculture as a potential alternative to not only increase strawberry tip yield but also to produce healthier, virus-free transplants (rooted tips). Growing stock plants indoors where the runners grow vertically downward increases the yield and quality of tips, however, there is a lack of information on the general architecture of plants especially the spatial distribution of daughter plants and also the leaf area index (LAI) distribution of the daughter plants’ canopy in the growing space. This information is vital for future system design as it determines the distance between the shelves and the potential need for intracanopy lighting. Furthermore, besides temperature, photoperiod strongly affects the trade-off between runner Vs. flower production. Yet, there is also a lack of information on how photoperiod will change the yield, quality, and architecture of stock plants in CEA, specifically on long-day cultivars. Here, we examined 12, 16, and 20 h photoperiods with the same DLI of 26 mol m-2 d-1 on ‘Monterey’ as a long-day cultivar with two replications in time. The chamber environment was maintained at a high temperature of 26 °C, ambient CO2, and 65% relative humidity. The plant density was 9 plant m-2. Following 64 days of growth, parameters related to yield, architecture, and quality were recorded. Results showed that even under relatively high temperatures, with shortening the photoperiod, a linear increase in the number of tips was observed, increasing from ~36.3 to ~44.3 (18%) with the same DLI. Regardless of the photoperiod, the highest proportion of tips (30.8%) were harvested on the runners from 40 – 80 cm distance from the mother plant, though the highest LAI (53.2%) was recorded on the tips from 0 – 40 cm, causing a sudden drop (92.8%) in the light intensity after 40 cm where most of the tips are growing. This information shows the need for intracanopy lighting under 40 cm depth from the mother plants. Around 98% of the tips were harvested from 0 – 160 cm, showing the needed space for the growth of runners (i.e., the distance between the shelves). Several morphology and photosynthetic parameters were also recorded. The information from this study will be used as a base for a follow-up experiment comparing top versus intracanopy lighting.
Speakers
avatar for Moein Moosavi

Moein Moosavi

PhD student, NC State University
Moein Moosavi-Nezhad is a Ph.D. student in the Horticultural Sciences department at NC State University, working in the Controlled-Environment Agriculture Lab under Ricardo Hernandez. He started working in CEA in 2016 at the University of Tehran focusing on plant-light interactions... Read More →
Co-authors
RH

Ricardo Hernandez

NC State University
NA
Wednesday September 25, 2024 9:00am - 9:15am HST
Coral 2

9:00am HST

CITR 2/HIST - Soil Amendment Strategies to Rehabilitate Freeze Damaged Citrus Trees for Sustainable Crop Management
Wednesday September 25, 2024 9:00am - 9:15am HST
Citrus in key citrus production states in the U.S. have been heavy hit from insect borne and disease pressures. The bacterial disease Huanglongbing (HLB) has had devastating impacts on the citrus industry in Florida. Despite having this threat to the Texas industry as well, the most recent negative impact to decrease citrus acreage has been due to harsh climatic events. In February 2021, citrus groves in south Texas were hit with icy weather that exposed orchards to prolonged freezing temperatures for several hours. The impacts of this one event reduced citrus orchards in south Texas by approximately 25% (over 2,800 ha or 7,000 acres). Climate variability has increased grower awareness to the potential for more frequent extensive drought and freezing winter periods. To better evaluate how to rehabilitate freeze damaged citrus trees, a two-year field study was created aimed at improving tree root health and soil conditions. Compost amendment (CA) was broadcast applied as well as application underneath the tree canopy of grapefruit trees in combination with varying nitrogen fertilizer rate application. Three fertilizer treatments: T1 (base rate = 112 kg N ha-1), T2=1.5x and T3=2x the T1 base rate were evaluated in a randomized complete block design with (11.2 Mg CA ha-1) and without CA in Rio Red grapefruits. Results indicated that root abundance significantly increased under composted trees compared to non-composted trees. Composted trees led to higher yields within 18 months following the freeze event compared to non-composted trees. The impacts of this research may aid Texas citrus growers and other producers looking to improve in-field management strategies that may assist in preparation for post-freeze weather events and extended seasons of water scarcity.
Speakers
SN

Shad Nelson

Dean and Professor, Texas A&M University Kingsville
Dr. Shad Nelson has serves as the Dean of the Dick & Mary Lewis Kleberg College of Agriculture & Natural Resource since 2016.  Prior to this he served as a Professor teaching Horticulture Science courses and research in alternative irrigation strategies for South Texas Citrus industry... Read More →
Co-authors
Wednesday September 25, 2024 9:00am - 9:15am HST
South Pacific 1

9:00am HST

FLOR 1 - Phosphorous Restriction Suppresses Growth of Containerized Coneflower
Wednesday September 25, 2024 9:00am - 9:15am HST
Containerized coneflower (Echinacea sp.) production in greenhouses and nurseries often relies on commercial fertilizers, such as 20 nitrogen (N)–4.4 phosphorous (P)–16.6 potassium (K), applied at 100 to 200 mg·L−1 N; however, increasing N concentrations proportionately increase phosphorous pentoxide (P2O5) and thus, elemental P concentrations. As such, the recommended N fertilization supplies P concentrations of 21.8 to 43.6 mg·L−1 P, exceeding plant requirements and potentially leading to excessive stem elongation and P runoff, posing ecological risks. Therefore, the objective of this study was to evaluate the effects of P concentrations on the growth, ornamental value, and tissue mineral nutrient concentrations of coneflower (E. × hybrida Sombrero® Granada Gold ‘Balsomold’). A custom soilless substrate was formulated with (by vol.) 55% aged pine bark, 35% sphagnum peatmoss, and 10% perlite, and amended with 0.6 kg·m−3 wetting agent and 3.9 kg·m−3 dolomitic limestone to achieve a final pH of ≈5.6 to 5.8. Young plants of coneflower were individually transplanted into 16.5-cm-diameter (1.7 L) containers filled with the custom soilless substrate. Upon transplanting and throughout the experiment, plants were irrigated with nutrition solutions formulated from technical grade salts providing 0, 2.5, 5, 7.5, 10, 15, 20, or 30 mg·L–1 P. Plants were grown in a glass-glazed greenhouse at 20 °C under ambient daylight supplemented with a photosynthetic photo flux density of ≈120 µmol·m–2·s–1 delivered from light-emitting diode lamps from 0600 to 2200 hr (16-h photoperiod) to achieve a daily light integral of 14 mol·m–2·d–1. At anthesis (≈69 d after transplant), data were collected. In general, plant height, plant diameter, and shoot and root dry weights were significantly influenced by P concentrations, although to different magnitudes. Quadratic plateau models demonstrated plant height, plant diameter, and shoot dry weight were maximized at 52.7 cm, 36.5 cm, and 27.2 g, respectively, with P concentrations of 18.3, 15.9, and 23.4 mg·L−1 P, respectively. Root dry weight demonstrated a positive linear correlation with increasing P concentration where roots were 74% (3.3 g) larger at a P concentration of 30 than 0 mg·L−1 P. Concentrations of ≤ 7.5 mg·L−1 P resulted low (≤ 0.2% P) leaf tissue P concentrations and ≤ 2.5 mg·L−1 P resulted in low-quality plants with incipient symptoms of P deficiency. Collectively, these results indicate that a narrow range of P concentrations may be used to control coneflower growth and mitigate P pollution.
Speakers
avatar for Garrett Owen

Garrett Owen

Extension Specialist and Assistant Professor of Sustainable Greenhouse & Nursery Systems, The Ohio State University
Dr. W. Garrett Owen is Assistant Professor and Extension Specialist of Sustainable Greenhouse and Nursery Production Systems with primary responsibilities in teaching (20%), research (30%), and Extension (50%) in the Department of Horticulture and Crop Science at The Ohio State University... Read More →
Wednesday September 25, 2024 9:00am - 9:15am HST
Nautilus

9:00am HST

GG 1 - Population Genetics and Genome-wide Association Studies Provide Insights into the Genetic Basis of Persea Fruit Quality Traits
Wednesday September 25, 2024 9:00am - 9:15am HST
Avocado (Persea americana) is renowned for its high nutritional value and its global consumption is steadily increasing. Currently, only a few cultivars with limited genetic variability are cultivated, and there is a need for developing new avocado cultivars with enhanced horticultural, fruit quality and nutritional traits as well as resistance to diseases and pests. Application of marker assisted selection can significantly accelerate breeding new avocado varieties, which can take 15 - 20 years using traditional breeding methods. Towards the application of molecular markers in avocado breeding, in this report, genome-wide association studies (GWAS) of nine fruit quality traits of a diversity panel of 110 avocado accessions were explored using 4,706 high-quality single nucleotide polymorphisms (SNPs) using multiple models. In addition, genetic diversity and population structure were also investigated, which unveiled three main populations corresponding to the three major avocado botanical races representing Mexican, West Indian, and Guatemalan ecotypes. Phylogenetic study and quantitative genetic analyses suggested a closer relationship between the Guatemalan and West Indian races compared to the Mexican race. Genome-wide association study revealed twelve markers distributed over eleven genomic regions strongly associated with fruit quality traits including fruit color, shape, taste, and skin texture. Annotation analyses of these genomic regions revealed candidate genes affecting these traits. These findings contribute to a comprehensive understanding of the genetic composition of avocado germplasm, which will be useful for identifying genes governing fruit quality traits as well as for accelerating breeding and parent selection efforts in the avocado breeding pipeline.
Speakers
GA

Gul Ali

Geneticist/Lead Scientist, USDA ARS Subtropical Horticulture Research Station
Co-authors
BF

Barbie Freeman

USDA ARS Subtropical Horticulture Research Station
NA
JL

Jin Li

USDA ARS Subtropical Horticulture Research Station
NA
SE

Shamseldeen Eltaher

USDA ARS Subtropical Horticulture Research Station
NA
Wednesday September 25, 2024 9:00am - 9:15am HST
Coral 1

9:00am HST

PGR 1 - Ethephon Effect on Blooming of Three Southern Highbush Cultivars
Wednesday September 25, 2024 9:00am - 9:15am HST
Spring frost is the primary cause of yield reduction in southern highbush, representing a significant challenge to blueberry production in the southeastern United States. Plant growth regulators are used as aids in different agricultural industries to either advance or delay growth and development process. Ethephon influences fruit set, size, and yield and has also been used as a tool to delay bloom in order to avoid freeze damage. This study examined the efficacy of ethephon to delay flower bud growth stages in three Southern Highbush Blueberry cultivars (‘Farthing’, ‘Georgia Dawn’, and ‘Kee Crisp’). Ethephon was applied at different concentrations of 200, 400, and 800 ppm, and a control treatment water/surfactant was also included. Flower buds were visually assessed from January until March. No relevant results were found for ‘Farthing’; however, for ‘Georgia Dawn’ and ‘Kee Crisp', ethephon at 800 ppm had the highest effect on delaying blooming development compared to the control. These results suggest that ethephon might delay flower bud growth stages in Georgia Dawn and Kee Crisp, offering potential advantages in reducing susceptibility to spring chill injury.
Speakers
LC

Lilian Carcamo

University of Georgia
Co-authors
MC

Mark Czarnota

University of Georgia
NA
MB

Melissa Brannon

University of Georgia
NA
ZR

Zilfina Rubio Ames

University of Georgia
Wednesday September 25, 2024 9:00am - 9:15am HST
South Pacific 3

9:00am HST

QUAL - Inhibiting Foodborne Pathogens and Biofilms on Fresh Produce by Nano-emulsions Developed from Lactobacillus-Derived Exopolysaccharide and Eugenol
Wednesday September 25, 2024 9:00am - 9:15am HST
The proliferation of foodborne pathogens and the formation of biofilms poses a major threat to the food industry, particularly in fresh produce. Raw and minimally processed fruits and vegetables are often sold to consumers in ready-to-use or ready-to-eat forms and are at the forefront of this risk. These products lack preservatives and rarely undergo any heat processing prior to consumption, making them more susceptible to contamination. To address this, we synthesized an oil/water emulsion encapsulating eugenol, using exopolysaccharide extracted from Lactobacillus as a natural emulsifier, and investigated its effectiveness against pathogenic cell populations within biofilm matrices. Different strains of Listeria monocytogenes, Salmonella, and E. coli were studied; results reveal substantial variations in biofilm-forming abilities among different E. coli, Salmonella, and L. monocytogenes strains. Specifically, L. monocytogenes V7 and Scott A strains displayed resistance to the emulsion, resulting in a minimal reduction of 2.8 log CFU/cm2 on the stainless-steel surface within the biofilm. Conversely, other strains exhibited reductions ranging from 3 to 4.5 log CFU/cm2. Most strains’ biofilm inhibition was 85% on plastic surfaces, whereas L. monocytogenes V7, Scott A, and Salmonella enterica ATCC 35664 showed less inhibition. The emulsion was applied to fresh produce (tomatoes, blueberries, and lettuce), where the reduction in E. coli and Salmonella biofilms, along with planktonic cells, varied from 0.53 to 5.1 log CFU/cm2, indicating that the emulsion significantly inhibited the growth of the tested pathogens. In conclusion, the results indicate that biofilm inhibition depends on the microbial species due to the observed individual variation in strains. However, combining eugenol and exopolysaccharide as emulsions is a promising natural alternative and sustainable technology for removing pathogenic biofilms. This research has the potential to advance food safety and hygiene standards, with practical applications in preserving fresh produce and disinfecting surfaces. This work was supported by USDA-NIFA- SCRI- 2017-51181-26834 through the National Center of Excellence for Melon at the Vegetable and Fruit Improvement Center of Texas A
Speakers
SB

SANGEETA BALYAN

Graduate Student, Texas A & M
Co-authors
Wednesday September 25, 2024 9:00am - 9:15am HST
Kahili

9:00am HST

TECH 1 - Effect of Innovative Laser Labeling Technology on Fresh Produce Quality and Safety
Wednesday September 25, 2024 9:00am - 9:15am HST
Introduction: Fresh produce is commonly associated with foodborne disease outbreaks and food recalls. To prevent the lethal impact of outbreaks, effective traceability is crucial. Produce items are traditionally labeled with price lookup (PLU) stickers. However, those stickers are environmental hazards, and frequent detachment of PLU stickers losses the information for traceability. Purpose: To investigate the effect of postharvest quality and microbial safety of laser labeling on fresh produce. Methods: Three horticultural crops, apple ‘Red Delicious ‘apple, cucumber, and green bell pepper, were procured from a local grocery store. Each produce was printed with a Quick Response (QR) code or text code using the laser engraver machine, followed by the application of edible wax. All produce was stored at 4° C temperature and 90% relative humidity during the study period. The postharvest quality was measured through fresh weight loss, QR code readability, and visual quality for 16 days. In another study, the laser-labeled produce was assessed for microbial contamination by quantifying artificially inoculated rifampicin-resistant E.coli (ATCC 25922) at an initial concentration of 106 CFU/mL. The experiment had five treatments: QR-coded labels followed by waxing or no wax, text-coded labels followed by waxing or no wax, and nontreated control. Results: Fresh weight loss for laser-printed produce was slightly higher than the nontreated control, but no difference in visual quality ratings was observed compared to the control. The population of rifampicin-resistant E.coli was statistically higher in all three produce labeled with text code compared to the nontreated control. However, QR-coded treatments were similar in the control. The application of wax did not facilitate microbial attachment. Significance: Laser labeling technology did not deteriorate the postharvest quality and susceptibility to microbial contamination. Hence this technology has the potential in commercial application as a better alternative to the PLU sticker to improve traceability.
Speakers
avatar for Manreet Bhullar

Manreet Bhullar

Kansas State University
Co-authors
DK

Durga Khadka

Kansas State University
NA
EP

Eleni Pliakoni

Kansas State University
MJ

Majid Jaberi Douraki

Kansas State University
NA
PA

Patrick Abeli

Kansas State University
NA
XX

Xuan Xu

Kansas State University
NA
Wednesday September 25, 2024 9:00am - 9:15am HST
South Pacific 2

9:00am HST

VCM 2 - Alternative Carbon Sources Effects on the Soil-plant Nutrient Dynamics During and Post ASD in a High Tunnel Tomato Crop System
Wednesday September 25, 2024 9:00am - 9:15am HST
Anaerobic soil disinfestation (ASD) is a microbial-driven soil disinfestation approach for the management of soilborne pests and pathogens of horticultural crops including protected vegetable production systems increasingly affected by emerging soilborne issues. ASD is implemented by incorporating in the soil an easily labile carbon (C) source, mulching the soil with an impermeable film, and irrigating the soil to field capacity. Organic amendments used as C source also provide N and other nutrients, and research is needed to assess if and how crop fertilization should be adjusted accounting for such nutrients. A study was conducted to evaluate the effect of alternative C sources on soil nutrient availability during and post-ASD in a high tunnel tomato production system. Carbon sources tested included sugarcane molasses, wheat middlings, and soybean meal mixed with wheat middlings. Each C source was applied at the standard rate of 6 Mg/ha of total C. The same C sources were also applied at half rate (3 Mg/ha of total C) balancing the total nitrogen (N) applied to 350 kg/ha of N using composted chicken manure as a source of N. The selected C source treatments were compared with untreated soil amended only with composted chicken manure and an untreated control to estimate the amount of nutrients provided by the untreated soil or by the composted chicken manure. Post ASD, fresh-market tomato cv Red Deuce was planted and used as a test crop evaluating plant growth, nutrient uptake, and yield and quality performance in response to soil treatments. Post-ASD, soil redox potential (Eh) was monitored revealing that all C sources tested determined a significant decrease of soil Eh, suggesting a good efficacy of the ASD treatment. During and post-ASD soil pH, EC, nitrate-N and ammonium-N levels were monitored using soil-water extracts (obtained with the 1:2 v:v method). Significant variation of soil pH, EC and mineral-N content were observed during and post-ASD, which influenced tomato marketable yield. Among the treatment tested WM applied at half rate and balanced for the N which had an intermediate C:N ratio (11.65) provided the highest seasonal marketable yield (10.4 kg/plant), while the untreated control amended with chicken manure provided the lowest yield. Overall, this study suggests that C application rate and the C:N ratio of the organic amendments used to apply ASD have significant effects on the efficacy of the ASD treatment as well as on the availability of nutrients and the crop performance post-ASD.
Speakers
FD

Francesco Di Gioia

Pennsylvania State University
Co-authors
FP

Fritzner Pierre

Pennsylvania State University
NA
Wednesday September 25, 2024 9:00am - 9:15am HST
South Pacific 4

9:00am HST

Root Growth and Rhizosphere Dynamics (RHIZ) Interest Group Meeting
Wednesday September 25, 2024 9:00am - 10:00am HST
Moderator
LT

Lisa Tang

USDA-ARS Appalachian Fruit Research Station
Wednesday September 25, 2024 9:00am - 10:00am HST
Sea Pearl 3

9:15am HST

CE 2 - Stratified Wood Substrates for Optimizing Growth of Greenhouse-Grown Strawberries and Blueberries
Wednesday September 25, 2024 9:15am - 9:30am HST
As the demand for locally grown produce, particularly fresh fruits like blueberries and strawberries, continues to surge, the imperative for year-round production becomes increasingly evident. While these fruits are globally recognized for their extended-season production potential in controlled environments, the intricate environmental factors crucial for improved production and profitability remain partially understood, including the impact of soilless substrates. Amid growing concerns about the availability of internationally sourced substrates, such as coconut coir, this study explores an alternative approach to reduce reliance on such components, particularly in the cultivation of two small fruits rapidly integrated into controlled environments. Substrate stratification, involving the vertical layering of substrates within a single container, has shown promise in nursery and greenhouse settings, enhancing resource efficiency in terms of water and fertilizer inputs. However, no research has assessed the application of stratified substrates for fruit crops with the specific aim of reducing coir inputs in greenhouse production. Thus, the objective of this study was to investigate whether stratifying coir over low-cost, hammer-milled processed tree fiber could effectively decrease coir usage, dependency, and associated costs within the controlled environment fruit production industry. 'Albion' strawberries and 'Star' blueberries were cultivated in five substrate treatments, ranging from 100% coir to various stratified layers beneath coir, including 25%, 50%, and 75% coir compositions, as well as a 100% processed tree fiber treatment. Results suggest that employing substrate stratification enhanced plant growth for both fruit crops, indicating its potential utility in optimizing controlled environment fruit production while reducing reliance on costly substrate materials like coir. Further analysis will elucidate the full implications of this innovative approach on production efficiency and profitability.
Speakers
BS

Brandan Shur

North Carolina State University
Co-authors
BJ

Brian Jackson

NC State
NA
Wednesday September 25, 2024 9:15am - 9:30am HST
Coral 2

9:15am HST

CITR 2/HIST - Physiological and Metabolomic Changes in Young Citrus Trees Under Elevated CO₂ and High Temperatures: A longitudinal st
Wednesday September 25, 2024 9:15am - 9:30am HST
As the atmospheric levels of CO₂ continue to rise, the concentration of carbon sources available for the plants also increases photosynthesis. The rise in atmospheric CO₂ is associated with global temperatures, resulting in an array of plant responses. Studies evaluating plant response to CO₂ treatments of 350 plant species show that plant responses are highly variable and species dependent, with the majority of studies being conducted on vegetable and grain crops, leaving a gap in understanding how tree crops respond. Mandarin (C. reticulata) trees are an economically important crop produced in California subject to climate change. This study used nursery trees of cv. Tango budded on C35 rootstock to determine the effect of elevated CO₂ exposure (400 ppm ambient vs. 800 ppm elevated) at elevated temperature (28°C ambient vs. 45°C elevated) to identify the physiological and metabolomic plant responses associated with each treatment. A longitudinal investigation was conducted over eight weeks in growth chambers (Conviron A1000, Winnipeg, CA) utilizing four treatment groups: ambCO2/Tamb (Control: CO2 400 ppm/Temperature 28°C); eCO2/Tamb (elevated CO2 at 800 ppm/Temperature 28°C); ambCO2/eTemp (CO2 at 400 ppm/Temperature 45°C); and eCO2/eTemp (CO2 at 800 ppm/ Temperature 45°C). Every two weeks, four replicates were collected from each treatment group. physiological measurements, photosynthesis response surveys, and metabolomic analysis were performed. Phenotypic measurements such as plant height, branches, leaf area, and leaf count showed that plant growth was impacted by treatment. Plant height, dry weight, and leaf count were significantly lower in the ambCO2/eTemp treatment as compared to the control (ambCO2/Tamb) as well as the other two treatments, indicating heat stress. Under elevated CO2 levels, trees were able to exhibit similar growth behaviors as the control treatment even under heat stress, signaling that the trees under eCO2 could compensate for a higher burden on carbon balance under heat stress. eCO2/Tamb trees accumulated significantly higher starch than all other treatments, whereas trees under both eTemp treatments showed significantly higher soluble sugars while significantly lower starch levels. This indicates that the trees under supplemental CO2 accumulate higher photoassimilates when they are free from heat stress, and the trees under heat stress exhibit starch conversion to soluble sugars as a stress response. Metabolomics analysis using Nuclear Magnetic Resonance (NMR) will provide valuable insight into the impact of treatment groups of eCO₂ and eTemp impact on Mandarin trees.
Speakers
avatar for Taylor Hornburg

Taylor Hornburg

Graduate Student, california state university
Co-authors
DG

Dave Goorahoo

California State University, Fresno
GB

GURREET BRAR

california state university
VK

Vishvanathan Krishnan

California State University
NA
Wednesday September 25, 2024 9:15am - 9:30am HST
South Pacific 1

9:15am HST

FLOR 1 - Investigating Synergistic Effects of Biostimulants and Biochar on Water Use Efficiency for Containerized Celosia (Argentea cristata) and Gomphrena (Gomphrena globose) Production
Wednesday September 25, 2024 9:15am - 9:30am HST
Given the escalating demands for water and the accompanying scarcity, enhancing water use efficiency (WUE) in horticultural practices has become imperative. Research has revealed that both biochar (BC) and seaweed extract biostimulants (BS) significantly enhanced WUE in plant growth, presenting promising avenues for sustainable agricultural advancement. This study aimed to investigate the synergistic effect of BC and BS on WUE for celosia (Argentea cristata) and gomphrena (Gomphrena globose) production. Three factors were included in this experiment including 1) mixed hardwood biochar blended with commerical substrate mixes (CS) at 0%, 10%, 15%, and 25% 2) 60% or 80% irrigation and 3) 0 or 5 mL BS(seaweed extract). The physical properties of substrate, including container capacity (CC), total porosity (TP), air space (AS), bulk density (BD), and water holding capacity (WHC), and chemical properties including leachate pH and electrical conductivity (EC) were measured. Plant growth parameters including growth index (GI) and leave greenness (indicated with SPAD value), biomass, and numbers of flowers were measured biweekly. Photosynthesis rate, transpiration rate, and stomatal conductance rate were measured at 6, 7, 8 WAT. The results showed that BC rates had significant effects on CC, and BD, but no effects on either TP, AS or WHC. Celosia plants grown in the 25% BC mix treated with 5mL BS at 80% irrigation had significantly higher biomass and WUE, and both plants in the same mix (25
Speakers
avatar for Lilin Chen

Lilin Chen

Graduate Research Assistant, University of Georgia
I'm interested in biochar effect on containerized plants, especially the effect under abiotic stresses such as drought and salinity.
Co-authors
PY

Ping Yu

University of Georgia
YC

Yulong Chen

The University of Georgia
Wednesday September 25, 2024 9:15am - 9:30am HST
Nautilus

9:15am HST

GG 1 - Genome Assembly of Persia Americana cv. Simmonds Provides Insights on Genetic Relationships Among Avocado Hybrids Exhibiting Tolerance To Laurel Wilt
Wednesday September 25, 2024 9:15am - 9:30am HST
Avocado (Persea americana) is the major fruit cultivated in southern Florida counties with a value exceeding 20 million dollars annually. While production in other regions is dominated by the Hass cultivar, south Florida is unique in production of the increasingly popular, green-skinned varieties. Recently, the avocado industry in South Florida has been devastated by laurel wilt (LW), an insect-disease complex spread by Raffaelea lauricola (Rf), a fungal symbiont of redbay ambrosia beetle (Xyleborus glabratus Eichhoff). Current management practices including prophylactic fungicide injections, tree rejuvenation and ambrosia beetle population reduction are costly and onerous . Unfortunately, no mature avocado trees tolerant to LW are available to growers and genetic mechanism of LW tolerance observed in some avocado seedlings is unknown. In this study, a chromosomal genome of avocado cv. ‘Simmonds’, a ‘West Indian’ (Lowland) ecotype was assembled from Pacific Biosciences HiFi reads. The genome assembly contained 451 scaffolds spanning 98.89% of the avocado genome, a N50 of 82.34MB and a BUSCO score of 95%. This assembly served as a reference genome to generate 9198 genome wide single nucleotide polymorphisms (SNPs) using genotyping by sequencing (GBS) reads of a germplasm collection comprising 80 accessions of three avocado ecotypes (Mexican, Guatemalan and West Indian) and 18 novel hybrids exhibiting seedling tolerance to LW. Phylogentic analyses revealed three major clusters with majority of LW tolerant seedlings clustering amongst Hass derived hybrids as well as cultivars belonging to Mexican and Guatemalan ecotypes such as 'Winter Mexican', and 'Ettinger'. This work provides genomic resources for characterization of genetic tolerance of LW in avocado germplasm collections and is a significant step in developing LW tolerant hybrids to support local avocado industry.
Speakers
VN

Vincent Njung'e Michael

University of Florida
Co-authors
JH

Jonathan H Crane

University of Florida, TREC
RG

Romina Gazis

University of Florida
NA
XW

Xingbo Wu

Chair 2023-2024, University of Florida
NA
Wednesday September 25, 2024 9:15am - 9:30am HST
Coral 1

9:15am HST

PGR 1 - Development of a Tissue Culture Approach for Doubling the Ploidy Level of Southern Highbush Blueberry Varieties
Wednesday September 25, 2024 9:15am - 9:30am HST
Increased consumption of blueberries has led to a prominent rise in demand. However, Georgia blueberry production is limited by challenges including short orchard life of southern highbush (SHB; tetraploid) and fruit quality issues associated with rabbiteye (RE; hexaploid) varieties. Interspecific hybridization can bring in beneficial alleles to improve the local adaptability and fruit quality of commercial varieties. Many of the wild blueberry germplasm with valuable soil adaptability and fruit quality traits such as V. fuscatum are diploids. Heteroploid crossings between SHB and diploid wild blueberry were shown to have a very low level of success. Ploidy induction through tissue culture is promising to circumvent the ploidy barrier and improve the efficiency of wide-hybridization. The objective of this research focuses on developing a ploidy induction protocol using two SHB varieties ‘Emerald’ and ‘Rebel’. SHBs previously initiated in tissue culture, were treated with 0%, 0.02% and 0.2% colchicine. SHBs were segmented into single and double node segments and put into woody plant medium (WPM) culture media containing either 6-(γ,γ-Dimethylallylamino)Purine (2iP) or trans-Zeatin. The treatments, along with non-treated control, were grown in a growth chamber of 26°C with 16/8 day and night cycles. The number and length of new shoots were recorded 35 and 50 days after treatment (DAT). Significant longer axillary stem growth was observed in the non-treated control compared to that of colchicine-treated segments for both SHBs indicating the suppressive effect of stem growth from colchicine treatments. Several Octo- and mix ploidy-shoots of ‘Emerald’ and ‘Rebel’ were identified among colchicine-treated SHB explants after ~ 21 weeks using a flow cytometer. Both levels of colchicine treatments generated octoploids. These new synthetic octoploid blueberries will be useful to cross with SHB and RE blueberries. The established ploidy induction protocol will be utilized to double the chromosomal levels of diploid V. fuscatum species and make them cross-compatible with SHB varieties.
Speakers
EW

Emily Walter

University of Georgia
Co-authors
YC

Ye Chu

University of Georgia
NA
Wednesday September 25, 2024 9:15am - 9:30am HST
South Pacific 3

9:15am HST

QUAL - Evaluating Nutritional Quality and Consumer Acceptability of Lettuce (Lactuca sativa L.) Grown with a Movable High Tunnel
Wednesday September 25, 2024 9:15am - 9:30am HST
Light quality is known to affect the growth and phytochemical content of numerous crops, including lettuce (Lactuca sativa L.). High tunnel production of lettuce provides many benefits to growers including increased yield due to higher soil temperatures and protection from abiotic stressors, such as strong wind and heavy rains. However, the use of UV-blocking polyethylene films can also result in lower antioxidant capacity and phenolic content, leading to a decrease in nutritional quality compared to lettuce grown in the open-field. Movable tunnels provide the ability to expose the crop to full-spectrum light once the crop has grown to full size. Our objective was to identify management strategies to optimize yield, phytochemical production, color, safety, and consumer acceptability of red-leaf cultivar (‘New Red Fire’). Lettuce was planted in a movable high tunnel April 2022 and exposed to 0, 2, 7, or 14 days of full sun prior to harvest. Total marketable yield, leaf color (L*a*b* color space), total phenolic content (TPC) and anthocyanin content were measured at harvest. Lettuce consumers (N=100) were asked to rate overall liking and various sensory attributes using continuous intensity scales, CATA, and open-ended comments. Total marketable yield and TPC were not affected. Leaf redness (a*) and anthocyanin content were significantly higher after 14, 7, and 2 days of full-sun exposure compared to 0 days. Significant differences were found in consumer liking for overall liking, color intensity, and ruffleness. The crop that was exposed to 14 days of full-spectrum light had a higher liking and more red color intensity. Using a movable tunnel system provides lettuce growers the ability to affect the color, phytochemical content, and consumer liking of their crop to varying degrees. This level of flexibility provides local, small-scale growers a crucial advantage in marketing their produce.
Speakers
CR

Cary Rivard

Kansas State University
Co-authors
EP

Eleni Pliakoni

Kansas State University
MB

Manreet Bhullar

Kansas State University
MT

Martin Talavera

Kansas State University
NA
OH

Olivia Haley

Kansas State University
NA
PA

Patrick Abeli

Kansas State University
NA
TJ

Tricia Jenkins

Kansas State University
Wednesday September 25, 2024 9:15am - 9:30am HST
Kahili

9:15am HST

TECH 1 - Cover Crop Decision Support Tools: Exploring the new suite of online cover crop tools
Wednesday September 25, 2024 9:15am - 9:30am HST
Cover crop recommendations can be complex based on regional factors and different growing conditions. In order to combat these challenges, the Precision Sustainable Agriculture team (PSA) developed online tools that are readily available for producers to help them optimize cover crops on their operation. Tools include a species and variety selector tool, seeding rate calculator, nitrogen calculator, and economic decisions tool. These platforms look to help producers find success with cover crops that fit their operation’s needs.
Speakers
EH

Esleyther Henriquez Inoa

Research Assist., North Carolina State University
Technologies in agriculture and Cover Crop breeding. 
Co-authors
SM

Steven Mirsky

USDA ARS BARC
NA
Wednesday September 25, 2024 9:15am - 9:30am HST
South Pacific 2

9:15am HST

VCM 2 - Advancing Pepper Production Beyond Seasonal Limits: Findings from a High Tunnel System Integrated with Biostimulant Applications
Wednesday September 25, 2024 9:15am - 9:30am HST
Pepper is an important specialty crop in the Southwest U.S., cherished for its economic importance, cultural history, and high nutritional values. Despite being a leading pepper producer in the U.S., challenges such as high temperatures, erratic weather patterns, and imports from Mexico have hindered both production and profitability of growers in this region. In response, alternative systems like high tunnels have emerged as viable solutions. The mild winter climate in Southwest Texas offers an ideal setting for winter pepper cultivation in high tunnels, potentially boosting growers' profitability with off-season market opportunities. Application of biostimulants, such as seaweed extracts, holds promise in enhancing cold tolerance and transplant success within high tunnel systems. This study investigates the feasibility of winter pepper production in high tunnels established in the Wintergarden region, assessing various cultivars based on yield and quality. The high tunnel maintained a range of 8-10°C higher temperatures than the open-field on chilly days without additional heating. Seven pepper cultivars were evaluated, with and without seaweed extracts. Seaweed-treated plants exhibited an overall higher stomatal conductance and leaf transpiration rates along with reduced leaf electrolyte leakage. Rio de Oro; a Santa Fe type pepper had the highest PSII efficiency and total fruit yield. Seaweed application also increased total yield and individual fruit weight in cultivar-specific manner. The total phenolic content of fruits was increased by seaweed application at the early maturity stage whereas total flavonoid content varied by cultivar and maturity stage. Despite facing freeze challenges at the early transplant stage, this first-year experiment demonstrated promising potential for off-season pepper production in high tunnels in our region.
Speakers
AN

ASMITA NAGILA

Graduate Research Assistant, Texas A&M university
Co-authors
Wednesday September 25, 2024 9:15am - 9:30am HST
South Pacific 4

9:15am HST

Postharvest Browning Disorders in Pear: A Multidisciplinary Perspective
Wednesday September 25, 2024 9:15am - 10:30am HST
Speaker: Dr. Bart Nicolai. Pear fruit are typically stored under controlled atmosphere (CA) conditions. When the O2 concentration is too low or the CO2 concentration too high, browning disorders may develop. The current hypothesis is that the diffusion resistance of the tissue limits the O2 supply and causes the respiration metabolism to stall and fermentation to be upregulated. The low energy yield of the fermentation pathways causes an energy crisis, and essential maintenance processes such as cell membrane repair are no longer possible. Eventually cells die and phenolic substrates are oxidised to brown colored patches.
In this presentation Dr. Nicolai will discuss how gas transport in fruit can be studied by both experimental and in silico techniques. He will show how X-ray microtomography techniques can be used to create 3-D representations of pear tissue at micrometer scale and how its microstructure affects gas transport. As the respiration and fermentation pathways provide the driving force for gas transport, the development of browning disorders of pear fruit is, hence, determined to a large extent by its microstructure and respiratory activity. This knowledge may inspire the development of future sensors to measure the susceptibility of batches of pear fruit to CA related browning disorders and facilitate cool store management.

Wednesday September 25, 2024 9:15am - 10:30am HST
Coral 5 - ASHS Hort Theater

9:29am HST

Temperate Tree Nut Crops 1 (NUTS 1)
Wednesday September 25, 2024 9:29am - 11:00am HST
Can Hedge-Pruning Reduce Water Needs In Southeastern United States Pecan Orchards? - Bailey Rayfield
Leaf Anatomical Traits and Water Use Efficiency in Four Pecan Cultivars - Sarahi Bracamontes
Performance of Five Early-harvest Pecan Cultivars in South Georgia - Patrick Conner
Enhancing Pecan Tree Resilience Against Spring Freeze Events: Insights from Secondary Bud Dynamics and Carbohydrate Analysis - Lu Zhang
Biochar in Pecan Orchards: Unraveling Water Stress Dynamics for Sustainable Irrigation Management - Jamin Miller
Evaluating Soil Management Impacts on the Pecan Orchard Mycobiome in the Semi-Arid Southwestern United States - McKenzie Stock
Moderator
PC

Patrick Conner

University of Georgia
Wednesday September 25, 2024 9:29am - 11:00am HST
Lehua Suite

9:30am HST

CE 2 - Effect of Light Intensity on Strawberry Runner Tips Propagated Indoors
Wednesday September 25, 2024 9:30am - 9:45am HST
Indoor propagation systems that use sole-source lighting in controlled environments can facilitate year-round production of disease-free, uniform strawberry liners. However, optimal conditions to propagate strawberry runner tips indoors are unknown. Runner tips of ‘Albion’ and ‘Fronteras’ strawberries were propagated indoors for 28 d under four photosynthetic photon flux density (PPFD) treatments: 75, 150, 225, or 300 ± 5 μmol·m–2·s–1 provided for 24 h·d–1 by white light-emitting diode fixtures. Runner tips were also propagated in a shaded greenhouse under mist. After propagation, plants were moved to a common greenhouse compartment and grown for 7 weeks to evaluate carryover effects on fruit yield (‘Albion’) or daughter-plant production (‘Fronteras’). Treatment responses were similar for both cultivars, except that shoot dry mass (DM) of ‘Fronteras’ followed a quadratic response with increasing PPFD, which peaked at 225 μmol·m–2·s–1. In contrast, shoot DM of ‘Albion’ linearly increased with increasing PPFD. Root DM of both cultivars also followed an increasing response with PPFD. However, there were no treatment differences in the number of shoots produced per plant or the length of the longest root. Interestingly, plants propagated under ≥150 μmol·m–2·s–1 had several dead shoots (up to 20%), likely attributed to plant stress. After the carryover phase, ‘Albion’ propagated under 225 or 300 μmol·m–2·s–1 were statistically different and produced the lowest fruit fresh mass compared to those grown in the greenhouse, whereas values were similar among plants propagated in the greenhouse or indoors under 75 or 150 μmol·m–2·s–1. No treatment differences were measured in the number of daughter plants produced by ‘Fronteras’. These findings suggest that although higher PPFD indoors promoted rooting and growth, plants propagated in the greenhouse were likely better adjusted to the dynamic greenhouse environment, which enabled them to reach the same growth and development of indoor-propagated plants at the end of the carryover phase.
Speakers
LD

Lian Duron

Purdue University
Wednesday September 25, 2024 9:30am - 9:45am HST
Coral 2

9:30am HST

CITR 2/HIST - Reports and research on incorporating cover crops in Florida citriculture, 1896 – 2024
Wednesday September 25, 2024 9:30am - 9:45am HST
Florida is perhaps the state in the U.S. most identified with citrus cultivation. The warm winters, abundant sunshine and significant rainfall are qualities which promote this industry, although other factors make producing these fruits on larger groves more arduous. These include limited nutrient concentrations, problematic drainage dynamics, and low organic matter and cation exchange capacity. Such issues combine to make citrus production in Florida highly dependent on external inputs of labor and material, both of which can be financially burdensome and demonstrate high volatility in price or availability from year to year. Cover crops have the potential to cost-effectively amend many soil issues in Florida citrus groves if they are established and maintained according to certain management practices. This principle was first presented at the Florida State Horticultural Society meeting in the late 1890s, and then clarified by Harold Hume in Citrus Fruits and their Culture in 1904. Later, the non-native legumes Crotolaria pallida and Crotolaria juncea were identified in multiple publications as soil-building cover crops which could be implemented into Floridian citrus production. Florida’s citrus production in the wake of World War II saw a decline in its incorporation of legume cover crops in favor of synthetic fertilizers. More modern research initiatives in Florida focused primarily on the mitigation of transmissible disease like Phytophthora root rot and citrus canker, a trend which has become more apparent following the discovery of citrus greening in 2005 and the stark decreases in yields this bacterial illness has been the primary cause of. In response, a number of Florida citrus growers have adopted cover crop mixes featuring legumes to supply disease-afflicted citrus roots with a sustainable and regular source of nitrogen. This presentation will review past literature and will also discuss current research on legume cover crops that have the potential to bolster citrus operations which is being conducted by individuals in the state of Florida.
Speakers
avatar for Lorenzo Rossi

Lorenzo Rossi

Associate Professor, University of Florida
Dr. Rossi’s research program focuses on improving root health and growth on cultivated crops, leading to the development of environmentally sound and effective management methods. He is a horticulturist with specific expertise related to plant stress physiology, root dynamics and... Read More →
Co-authors
JP

Joseph Paoli

University of Florida
I am a Master's student at the University of Florida based at the Indian River Research and Education Center (IRREC). I am a long-time Florida resident, having gone to Middle and High School in Central Florida before enrolling at the University of Florida. During my four years of... Read More →
LH

Lukas Hallman

University of Florida
Wednesday September 25, 2024 9:30am - 9:45am HST
South Pacific 1

9:30am HST

FLOR 1 - Effects of Reduced Water Usage During Production on Economic Value, Growth, And Quality of Flowering Indoor Plants
Wednesday September 25, 2024 9:30am - 9:45am HST
The objective of this study was to investigate the effects of soil moisture content during greenhouse production of selected floriculture crops. Over the course of several seasons, multiple flowering species were investigated, including Euphorbia milii, Cyclamen persicum, and Begonia x tuberhybrida. Plant quality characteristics were evaluated both during the production timeframe, and after a simulated shipping and retail setting. Treatments included two soil moisture content levels (20% and 40%), which was monitored by soil moisture probes. Production timelines varied, due to the differences in species. Once the predetermined production weeks were concluded the plant went into simulated shipping and retail environment. Qualities such as growth index (GI), leaf greenness (SPAD), Leaf thickness, petiole thickness, irrigation events, and irrigation amount were measured weekly. Flower number and foliage fresh /dry weights were gathered after the two week simulated retail period. Results varied depending on the species. In all species the 20% group had less watering events, which equates to producer savings in fertigation inputs as well as labor. All species had reduced canopy size in the 20% treatment, which could equate to bench space conservation. Some of the plant quality characteristics depending on the species had similar results after simulated retail. This equates to the idea that producers could reduce water application events and volumes and generate an equal quality plant compared to the traditional watering methods, while also saving on input and labor costs.
Speakers
KH

Kaitlin Hopkins

Sam Houston State University
Wednesday September 25, 2024 9:30am - 9:45am HST
Nautilus

9:30am HST

GG 1 - Utilizing Haploid Pollen Grains and Diploid Leaf Tissue Genomic Sequence Data to Phase the ‘Wonderful’ Pomegranate Genome
Wednesday September 25, 2024 9:30am - 9:45am HST
The scientific and commercial interest in pomegranate (Punica granatum L.) cultivation has increased noticeably during the last two decades. Because of the high concentration of bioactive compounds and its nutraceutical properties, pomegranate has been defined as a super food. The consumption of pomegranate juice or arils has been related to several possible benefits on human health. Recent studies have highlighted an antioxidant and anti-inflammatory activity of this fruit which seem to prevent cardiovascular, neoplastic, neurological, metabolic, and intestinal disease. The areas of cultivation of this crop are exposed to current and future challenges like long term-drought conditions and invasive pests and diseases. Increasing the biodiversity of pomegranate has been proposed has the main strategy to reduce the risk of food system vulnerability related to monoculture and the valorization of marginal land. In order to develop advanced genetic tools to improve pomegranate breeding program efficiency we present the de novo sequencing of the ‘Wonderful’ pomegranate genome. DNA isolated from diploid leaf tissues was sequenced using long read sequencing technology (PacBio), while DNA extracted from haploid pollen grains was sequenced using short reads (Illumina). Genomic data from single haploid gamete cells were analyzed using the R package called ‘Hapi’. This allowed to infer chromosomal haplotypes obtaining a higher resolution for DNA variants detection and investigating recombination events in single gametes. Although ‘Wonderful’ represents the industry standard in the United States, several cultivars with desirable traits, such as low acidity and soft seednesses, have been identified in the national germplasm. The results of this study will provide the genomic data required to investigate differences among cultivars and create trait-gene associations. This will allow breeders to facilitate the integration of desired quality traits into new germplasm resources.
Speakers
GL

Giuseppe Lana

University of Florida
Co-authors
DS

Danelle Seymour

UC-Riverside
NA
DM

Donald Merhaut

UC-Riverside
NA
HQ

Han Qu

UC-Riverside
NA
JC

John Chater

University of Florida
MR

Mikeal Roose

UC-Riverside
NA
RT

Ryan Traband

UC- Riverside
NA
TB

Taylor Beaullieau

UC-Riverside
NA
ZJ

Zhenyu Jia

UC-Riverside
NA
Wednesday September 25, 2024 9:30am - 9:45am HST
Coral 1

9:30am HST

NUTS 1 - Can Hedge-Pruning Reduce Water Needs In Southeastern United States Pecan Orchards?
Wednesday September 25, 2024 9:30am - 9:45am HST
Pecan (Carya illinoinensis) trees have experienced higher density plantings which enhances the need for better water use efficiency to increase the sustainability of the orchard. The implementation of hedge-pruning allows for better light penetration as well as more efficient water use in the humid climate of the southeastern United States. The objectives of this study were to determine if irrigation rates can be reduced on hedge-pruned pecan trees with no loss in pecan yield or nut quality. The study is a split-plot design with pruning serving as the main plot effect and irrigation serving as the split plot effect. On hedge-pruned trees, all growth beyond 8’ from the trunk on the East side of the tree was pruned in year 1 and on the West side of the tree in year 2. Trees were topped on each side in their respective years at an angle with a peak at 40’. No pruning will be done in year 3. Hedging treatments are arranged in three tree blocks with each irrigation treatment occurring once per block as follows: 1) 100% irrigation; 2) 50 % irrigation; 3) non-irrigated control. Hedged blocks were replicated four times, and the non-hedged blocks were replicated three times. Among the irrigation regimes, there was no statistical difference between treatments indicating that, under the environmental conditions observed, less water is sufficient for pecan production, regardless of pruning treatment. Although, there has been no improvement in yield from the hedge-pruned trees, percent kernel has increased in the hedged trees compared to the non-hedged trees, suggesting an enhancement of pecan nut quality with hedge pruning.
Speakers
BR

Bailey Rayfield

University of Georgia
Co-authors
LW

Lenny Wells

University of Georgia
NA
Wednesday September 25, 2024 9:30am - 9:45am HST
Lehua Suite

9:30am HST

TECH 1 - CFD-based aerodynamic analysis under high wind velocity environment for multiple greenhouses
Wednesday September 25, 2024 9:30am - 9:45am HST
In South Korea, approximately 65% of the land is mountainous or forested, which limits large-scale farming. Over 53,000 ha of land has been reclaimed from the sea and dedicated to the development of large-scale indoor agricultural complexes. Given the coastal climatic conditions and flat nature, these areas present unique challenges including stronger winds and colder temperatures compared to the inland, leading to high air velocities and operation costs in naturally ventilated greenhouses. Aerodynamic analysis is necessary to estimate crop risk factors and identify potential aerodynamic problems before the construction of these structures. Traditional studies have focused on using natural ventilation rates to estimate greenhouse suitability for plant growth. However, under scenarios of high wind velocity, this approach has critical limitations in accounting for crop damage resulting from high air velocity induced inside naturally ventilated facilities. This is tailored to the fact that ventilation efficiency in naturally ventilated structures increases with an increase in air velocity. High wind velocity induced inside greenhouses is associated with rapid CO2 depletion, stomatal dysfunction, leaf abrasion, mechanical stress etc., which critically affect crop yield and biomass development. Under high wind environment, crop damage resulting from high internal air velocities is an important factor that needs to be accounted for during design of indoor agricultural facilities. This study introduces a CFD model for designing greenhouse complex including multiple greenhouses and model analysis approach. We developed the Aerodynamic Crop Damage Index (ACDI), used it to analyze the model, and compared it to the convectional ventilation efficiency approach. The ACDI revealed a 2.2-fold variation in damage potential based on the greenhouse's location within the complex and a 15-fold variation attributable to wind direction, pinpointing significant damage risks in zones with the highest and lowest air velocities. In contrast, the convectional ventilation efficiency method only identified damage risks in low-velocity areas. ACDI has not only the potential to account for high air velocity effects in naturally ventilated greenhouses but also presents an opportunity for specialized greenhouse complex control and management according to greenhouse position and incoming wind direction. Future research should aim at refining the ACDI for better aerodynamic analysis in greenhouse complexes planning and its integration into greenhouse ventilation control systems.

Acknowledgments: This work was supported by “Regional Innovations Strategy (RIS)” through the National Research Foundation of Korea (NRF) funded by Ministry of Education (MOE) (2024RIS-008)
Speakers
AK

Anthony kintu Kibwika

phd student, Jeonbuk National University, Korea
Co-authors
IS

Il-Hwan Seo

Associate Professor
Wednesday September 25, 2024 9:30am - 9:45am HST
South Pacific 2

9:30am HST

VCM 2 - Optimizing Nitrogen Fertilizer Rate and Planting Density of Vidalia Onion Yield and Bulb Size Distributions
Wednesday September 25, 2024 9:30am - 9:45am HST
This study was carried out at the University of Georgia (UGA) Vidalia Research Center during the 2022/2023 winter season and is being repeated in 2023/2024. The objectives of this experiment were to identify the relationship between planting density, nitrogen fertilizer rates, and the effects on yields and bulb size distribution. The variety “Sweet Magnolia” was tested at three planting densities (116,000; 87,000; and 58,000 plants per acre) and three nitrogen rates (120, 100, and 80 lbs. per acre). Results indicated there is no interaction between planting density and nitrogen rates on onion bulb size distribution or total production. However, the effect of planting density was significant. Higher densities (116,000 plants/acre) yielded a total weight of 1788 40 lbs. bags/acre with a significant proportion of jumbo-sized bulbs 62%, while lower densities (58,000 plants/acre) resulted in larger colossal bulbs 290 40 lbs. bags/acre with a reduced total yield 1370 40 lbs. bags/acre. Nitrogen application also had a significant impact on both yield and size of the bulb. The highest nitrogen rate (120 lbs./acre) produced a higher total yield (1670 40 lbs. bag/acre), with a significant portion of jumbo at 50% and colossal bulbs at 15%. There was no significant difference between the nitrogen rates of 100 and 120 lbs./acre, but the lowest nitrogen rate (80 lbs./acre) had the worst yield with 1434 40 lbs. bags/acre resulting in a 15% decreased yield compared with the higher rate. These results suggest that optimal planting density and nitrogen application are decisive for reaching the desired bulb size distribution and maximizing yield.
Speakers
avatar for Elvis Pulici

Elvis Pulici

MS Graduate Research Assistant, University of Georgia
Co-authors
HY

Henry Y Sintim

University of Georgia
NA
JC

Juan Carlos Diaz Perez

University of Georgia
TM

Ted McAvoy

University of Georgia
NA
Wednesday September 25, 2024 9:30am - 9:45am HST
South Pacific 4

9:30am HST

Coffee Break
Wednesday September 25, 2024 9:30am - 10:00am HST
Wednesday September 25, 2024 9:30am - 10:00am HST
Coral 4/5 - Conference Central

9:44am HST

Viticulture and Small Fruits 2 (VSF 2)
Wednesday September 25, 2024 9:44am - 11:15am HST
Evaluation of Pierce's Disease-resistant Grapevine Cultivars in South Carolina - Annie Bruno
Diversity and Abundance of Bees Visiting Grape Flowers in Wisconsin Vineyards - Christelle Guedot
The Grape Health Index: Validation of a New Methodology for Quantifying Wine Grape Spoilage by Means of FT-MIR Spectroscopy - Sonet Van Zyl
World Grape Rootstocks Pedigree Review - Alireza Rahemi
Genome Database for Vaccinium: Genomics Data and Tools to Facilitate Research - Jodi Humann
Use of Glycine Betaine and Kelp Extract Mitigates Heat Stress in Red Raspberry (Rubus idaeus) - Givemore Munashe Makonya

Moderator
SV

Sonet Van Zyl

Fresno State
Wednesday September 25, 2024 9:44am - 11:15am HST
Kahili

9:45am HST

CE 2 - Characterizing the Growth, Morphology, Productivity, and Fruit Quality of Twenty-five Strawberry Cultivars in Vertical Farm Environment
Wednesday September 25, 2024 9:45am - 10:00am HST
As the interest in strawberry production in controlled environment agriculture is ascending, the demand for cultivars that yield premium-quality fruit is rising. To identify strawberry suitable for vertical farm production, 25 strawberry (Fragaria × ananassa) cultivars were selected for premium flavor from USDA National Clonal Germplasm Repository. Fruit productivity and quality traits, plant vegetative phenotypes, and photosynthetic rates were evaluated using strawberries grown in a walk-in growth chamber where photoperiod altered between short day and long day to promote flowering and fruit production. Our results show that strawberry ‘Mara des Bois’ produced the earliest harvest, and ‘Hood’ had the highest maximum productivity coefficient. The largest fruit was produced by ‘Chandler’, and the reddest fruit was produced by ‘Marshall’. Among the 25 cultivars, 11 exhibited fruit Brix levels above 0.9, and 3 had a fruit Brix:TA ratio of 1.0. Ongoing fruit flavor analysis aims to identify unique flavor compounds within these strawberries. Correlations linked time to first harvest and maximum productivity coefficient with canopy area, shoot height, and photosynthetic rate per plant, revealing the intricate sink-source dynamics in strawberry plants. Interestingly, no correlation was found between maximum productivity coefficient and any fruit quality trait, challenging the commonly held belief in the constant competition between crop productivity and quality. The information of strawberry growth and production in vertical farm environment provided in this study can assist indoor growers in cultivar selection and potentially contribute to future strawberry breeding programs.
Speakers
YL

Yiyun Lin

The Ohio State University
Co-authors
CK

Changhyeon Kim

The Ohio State University
NA
CK

Chieri Kubota

The Ohio State University
NA
JO

Jim Oliphant

US Department of Agriculture
NA
MH

Michael Hardigan

US Department of Agriculture
NA
NB

Nahla Bassil

US Department of Agriculture
NA
Wednesday September 25, 2024 9:45am - 10:00am HST
Coral 2

9:45am HST

NUTS 1 - Leaf Anatomical Traits and Water Use Efficiency in Four Pecan Cultivars
Wednesday September 25, 2024 9:45am - 10:00am HST
The leaf surface and interior structure can affect photosynthesis and transpiration rates associated with water use efficiency. Several studies have assessed pecan (𝘊𝘢𝘳𝘺𝘢 𝘪𝘭𝘭𝘪𝘯𝘰𝘪𝘯𝘦𝘯𝘴𝘪𝘴) leaf anatomical traits and established differences between cultivars; however, the effect of these traits on water use efficiency across cultivars has not been established. Understanding the relationship between leaf anatomical structures and water use efficiency across pecan cultivars allows for the identification of those that are well suited for water limited environments. In this study, we examined the leaf mesophyll layer thickness (µm), trichome density (trichome mm⁻²), stomatal density (stomata mm⁻²), and stomatal pore area (µm²) of four pecan cultivars (‘Wichita’, ‘Western’, ‘Pawnee’, and ‘Lakota’; all grafted to clonally propagated rootstocks). Leaves were collected at the NMSU Leyendecker Plant Science Research Center, and their cross-section and abaxial surface were imaged using light and scanning electron microscopy, respectively. ‘Lakota’ leaves had the thinnest palisade mesophyll layer and thickest spongy mesophyll layer relative to total leaf thickness. The stomatal density of ‘Pawnee’ was significantly less than the other cultivars, but no significant differences in stomatal pore area were observed. Intrinsic water use efficiency, calculated as ratio of the carbon assimilation rate to stomatal conductance to water vapor, was obtained using a leaf gas exchange meter on three separate days. A stable carbon isotope composition analysis was also conducted to provide insight into the leaf’s longer-term water use efficiency. The results showed that the intrinsic water use efficiency of ‘Lakota’ was different from ‘Western’ on one of the days; however, differences between cultivars were not significant when averaged across the measurement dates. Furthermore, the relative ¹³C abundance and ¹³C discrimination did not have significant differences across the four pecan cultivars evaluated, suggesting that differences in leaf anatomical traits did not influence the water use efficiency of these samples.
Speakers
SB

Sarahi Bracamontes

New Mexico State University
Co-authors
CV

Ciro Velasco-Cruz

New Mexico State University
NA
JR

Jennifer Randall

New Mexico State University
NA
KG

Kanchan Grover

New Mexico State University
NA
RH

Richard Heerema

New Mexico State University
NA
SB

Samy Belteton

New Mexico State University
NA
Wednesday September 25, 2024 9:45am - 10:00am HST
Lehua Suite

9:45am HST

VCM 2 - Assessing the Efficacy of Plant Biostimulants on Onion Growth and Production in Greenhouse Trials and Field Conditions
Wednesday September 25, 2024 9:45am - 10:00am HST
The escalating impacts of climate change on agriculture necessitate the exploration of sustainable crop enhancement methods. This research integrates greenhouse screening and field application to evaluate the efficacy of biostimulants in promoting the growth of onion (Allium cepa L.) under variable environmental conditions. In the initial greenhouse experiment, seven biostimulants were tested, including three bacterial products (Continuum, Spectrum DS, and Tribus Original), two mycorrhizal products (Mighty Mycorrhizae and Myco Apply), one seaweed extract product (Seaweed), and one product containing humic acid (Huma Pro 16). These were applied to assess their impact on seedling emergence and growth in a peat-based soilless substrate. Huma Pro 16 and Spectrum DS were identified as the most effective in increasing seedling emergence, leaf area, and biomass. Based on these findings, field trials were conducted with four onion cultivars (red, sweet, white, and yellow), subjected to conditions simulating drought and normal watering (75 % and 100 % evapotranspiration) or with nitrogen levels adjusted to low and high (75 % and 100 % of optimal 168 kilograms per hectare). The field trials assessed bulb diameter, weight, and leaf metrics. Results indicated that cultivars responded differently to varying conditions, with red and white onions showing consistent growth patterns, while sweet onions displayed more variability. Yellow onions benefited from the biostimulants, exhibiting improved growth under both drought and low nitrogen conditions. This study demonstrates that the application of certain biostimulants can significantly enhance crop growth and resilience. Huma Pro 16 and Spectrum DS show promise for improving onion yield in the face of climatic challenges. Keywords: biostimulants, drought stress, nitrogen availability, onion, and sustainable agriculture.
Speakers
PN

PRAKRITI NEPAL

Utah State Univeristy
Co-authors
DT

Daniel T Drost

Utah State University
NA
YS

Youping Sun

Utah State University
Wednesday September 25, 2024 9:45am - 10:00am HST
South Pacific 4

9:45am HST

VSF 2 - Evaluation of Pierce's Disease-resistant Grapevine Cultivars in South Carolina
Wednesday September 25, 2024 9:45am - 10:00am HST
Recently, there has been an increased interest in wine grape (Vitis vinifera) production in South Carolina (SC). The warm and humid climate in SC creates a conducive environment for a high risk of disease incidence. Despite the typical climate, SC has droughts periodically. Pierce’s Disease (PD), caused by the xylem-limited bacterium Xylella fastidiosa (Xf), is a major limiting factor to grapevine cultivation throughout the United States and, specifically, in the southeastern U.S. However, PD-resistant grapevine cultivars have been released by UC Davis. Xf-infected grapes can be heavily influenced by water deficit. Preliminary data from a drought-stress experiment in the Summer of 2023 indicated two PD-resistant cultivars perform well under a water deficit. The objective of this study was to evaluate the water dynamics and gas exchange of PD-resistant cultivars during periods of drought. In March 2023, five PD- resistant cultivars and one French-American hybrid were planted in a variety trial at Musser Fruit Research Center in Seneca, SC. The drought-stressed PD-resistant ‘Paseante Noir’ and ‘Errante Noir’ had similar carbon dioxide assimilation to well-watered PD-resistant cultivars. PD-resistant cultivar ‘Ambulo Blanc’ showed the worst leaf water status and lowest carbon dioxide assimilation when stressed. In 2024, physiological measurements, such as sap flow, trunk diameter, gas exchange, and water potential, were taken throughout the growing season. Our results supported previous research that some PD- resistant grapevine cultivars perform well in the field under drought conditions.
Speakers
avatar for Annie Bruno

Annie Bruno

Grad Student, Clemson University
Wednesday September 25, 2024 9:45am - 10:00am HST
Kahili

9:59am HST

Consumer Horticulture and Master Gardeners 1 (CHMG 1)
Wednesday September 25, 2024 9:59am - 11:45am HST
Fruit Classes Teach Invasive Species Management and IPM Practices to Homeowners, Master Gardener Volunteers, and Pesticide Applicators - Kaydie McCormick
Invasive Potential of Ornamental Landscape Plants in Southern Arizona - Michael Chamberland
Education on Water Saving Landscaping Practices Results in Measurable Water Conservation - Tiare Silvasy
Cultivating Connections: Empowering Professionals through NC Farm to School Training Initiative - Liz Driscoll
The gARTening Program: Fostering Community Engagement through Innovative Education - Clarissa Chairez
Welcome Home to Gardening in Tennessee: Reaching New Audiences with New Collaborative Outreach Models - Natalie Bumgarner
Enhancing Backyard Fruiting Cultivation Efficacy Through Florida-Friendly Landscaping™ Techniques for Homeowners - Brittany Council-Morton

Moderator
KM

Kaydie McCormick

Extension Agent, University of Florida Institute of Food and Agricultural Sciences
Wednesday September 25, 2024 9:59am - 11:45am HST
South Pacific 2

9:59am HST

Ornamentals/Landscape and Turf 1 (O/LT 1)
Wednesday September 25, 2024 9:59am - 12:00pm HST
BeeGardens Mobile Application Improves Pollinator Plant Knowledge Gain in Landscaping and Gardening Courses - Sandra Wilson
Pesticide Management Decisions Affect Contamination of Nectar in Containerized Ornamental Plant Production - Patrick Wilson
Comparing Pollinator Species Richness and Abundance Between Pycnanthemum Species and Accessions - Kaitlin Swiantek
The Art and Technique of Producing Unique Lagerstroemia Plants - Donglin Zhang
Effects of Different Pruning Regimes on Growth Reallocation and Carbon Storage in Buxus microphylla var. japonica ‘Winter Gem’ - Andrew Loyd
Establishment, Growth, and Physiology of Container-Grown Trees Following Root Remediation at Planting - Bert Cregg
The Effects of Mulch Color and Depth on Soil Temperature and Light Transmission - Damon Abdi
Subterranean Termite Landscape Mulch Consumption Challenge - Edward Bush

Moderator
DZ

Donglin Zhang

University of Georgia
Wednesday September 25, 2024 9:59am - 12:00pm HST
Coral 1

9:59am HST

Postharvest 2 (PH 2)
Wednesday September 25, 2024 9:59am - 12:30pm HST
Postharvest Performance of Tomato Fruit at Shelf-life Conditions in Relation to Fertilization Rate During Plant Production and Fruit Ripening Stage at Harvest - Sotirios Tasioulas
Identification of NAC Transcription Factors Involved in the Ripening and Senescence of Pepper (Capsicum annuum L.) Fruit - Kyeonglim Min
Comparative Analysis of Volatile Organic Compounds of Eight Microgreens in the Brassicaceae Family - Jinhe Bai
Nutritional Quality and Shelf-Life of “Living Lettuce” Through 28 Days of Cold Storage - Marlee Trandel Hayse
Comparing the Postharvest Phytonutrient Content of Red and Green Butterhead Lettuce Cultivars - Marlee Trandel Hayse
Production System Alters Phytonutrient Content of ‘Skyphos’ Butterhead Lettuce - Marlee Trandel Hayse
Improving Postharvest Storage and Late-Season Production of Caladium Tubers in Florida Through Chemical Treatments - Gasselle Cordova
Impact of Storage Environment on Dormancy Progression and Microbiome of Potato Tubers - Munevver Dogramaci
Strawberry Anthocyanins, Extraction, Assessments and Storage - Toktam Taghavi
Isolation of Pathogenic Strain of Trichoderma atroviride from Symptomless, Disease-Free Sweetpotato (Ipomoea batatas L.) Storage Roots - Richard Noel Torres

Moderator
avatar for Munevver Dogramaci

Munevver Dogramaci

United States Department of Agriculture
Wednesday September 25, 2024 9:59am - 12:30pm HST
Nautilus

10:00am HST

CHMG 1 - Fruit Classes Teach Invasive Species Management and IPM Practices to Homeowners, Master Gardener Volunteers, and Pesticide Applicators
Wednesday September 25, 2024 10:00am - 10:15am HST
Florida's diverse climate offers ample opportunities for cultivating various fruit crops, especially as traditional citrus faces disease challenges. However, accessible information on alternative fruit crops is limited. In response, a series of online classes was initiated in 2020 to address this gap, coinciding with the need for Continued Education Units (CEUs) for pesticide license holders during COVID-19 lockdowns. The objectives of the class series were threefold: to enhance knowledge of fruit crop cultivation in Central Florida, promote the adoption of Best Management Practices (BMPs) in crop selection and care, and provide CEUs for pesticide license holders in Florida. In its pilot year, three Extension Agents developed a five-week class, expanding to a six-week series in 2021 involving five Agents across three counties. Each session focused on BMPs for landscapes and the cultivation of at least three different fruit crops, including discussions on potential invasive pests as well as alternatives to invasive fruit trees that shouldn’t be planted. The classes were conducted via Zoom and archived on YouTube for wider accessibility. Evaluation of the program included a post-class survey to assess knowledge gain and a three-month follow-up to measure the adoption of practices. Over the course of 2020-2023, the series covered care techniques for 29 different fruit crops. The impact of the classes has been significant. Since 2020, 23 episodes have been produced, attracting a total of 2,015 live viewers. Participants earned a total of 361 CEUs. Survey results indicated a substantial knowledge gain, with 96% (1,480/1,540) of respondents reporting increased understanding of the material. Moreover, 79% (195/248) of respondents adopted at least one new Integrated Pest Management (IPM) strategy after taking the class. According to that same survey 48% (98/202) have altered turf to fruit trees, planting a total of 37,477 square feet with edible plants. In summary, the online class series successfully addressed the demand for information on alternative fruit crops in Florida while providing valuable CEUs for pesticide license holders. Its impact is evidenced by increased knowledge among participants and tangible changes in cultivation practices towards more sustainable and diverse fruit production.
Speakers
KM

Kaydie McCormick

Extension Agent, University of Florida Institute of Food and Agricultural Sciences
Co-authors
MP

Morgan Pinkerton

UF/IFAS Extension Seminole County
TS

Tiare Silvasy

UF/IFAS Extension Hillsborough County
NA
TM

Tina McIntyre

UF/IFAS Extension Seminole County
NA
WL

William Lester

UF/IFAS Extension Hernando County
Wednesday September 25, 2024 10:00am - 10:15am HST
South Pacific 2

10:00am HST

NUTS 1 - Performance of Five Early-harvest Pecan Cultivars in South Georgia
Wednesday September 25, 2024 10:00am - 10:15am HST
Five pecan (Carya illinoinensis) cultivars were evaluated over 15 years in a trial at Tifton, GA, USA. Trialed cultivars included the standard early cultivar Pawnee, and the more recent releases Byrd, Morrill, Lakota, and Treadwell. Actual yield were measured for each tree each year and a 50-nut sample was taken to determine nut quality. Trees were evaluated for leaf and nut scab infection (Venturia effusa) and black aphid (Melanocallis caryaefoliae) damage. ‘Pawnee’ yielded significantly less than all the newer cultivars which had similar cumulative yields. However, ‘Byrd’, ‘Lakota’, and ‘Treadwell’ had significantly more yield alternation than ‘Pawnee’ and ‘Morrill’, with reduction of nut quality in the “ON” years. ‘Pawnee’ was the earliest cultivar and produced excellent quality nuts, but yields were mediocre. ‘Lakota’ had excellent scab resistance, but overcropped resulting in inferior quality, and should only be grown in orchards were crop loads are controlled by hedging or tree shaking. ‘Byrd’ and ‘Treadwell’ are very scab susceptible and also need crop load control, making them poorly suited for south Georgia. ‘Morrill’ had consistent cropping and excellent nut quality but is also very scab susceptible and should only be grown with excellent disease management practices.
Speakers
PC

Patrick Conner

University of Georgia
Wednesday September 25, 2024 10:00am - 10:15am HST
Lehua Suite

10:00am HST

O/LT 1 - BeeGardens Mobile Application Improves Pollinator Plant Knowledge Gain in Landscaping and Gardening Courses
Wednesday September 25, 2024 10:00am - 10:15am HST
Florida is home to over 300 species of native wild bees, some in critical decline. To encourage gardeners to plant bee friendly species that support bee pollinators year-round, an online application called BeeGardens was built using a shared library of code and a relational database management system. The application, accessible by a mobile device or computer, enables users to quickly access over 85 bee-friendly plants that attract 12 primary bee groups; and provides tips for incorporating these into different landscape designs (https://ffl.ifas.ufl.edu/bees). The functionality and usefulness of the app was evaluated by students enrolled in two courses at the University of Florida: Florida Native Landscaping and Annual and Perennial Gardening, taught in different semesters. Before and after the semester, students were asked to report their abilities to 1) identify bee-friendly plants, 2) identify bee pollinators, and 3) design a bee-friendly garden, using a Likert scale with responses ranging from 1 (strongly disagree) to 5 (strongly agree). Means of pre- and post-test responses showed a significant perceived knowledge gain upon using the BeeGardens online application in both courses. This data was consistent with pre- and post-tested means where students were asked to identify three major pollinator plants and three major pollinators using multiple choice response options. Test scores increased by 26.3% and 37.9% in Annual and Perennial Gardening and Florida Native Landscaping, respectively. The majority of students (95.0%) agreed or strongly agreed this learning tool was organized, easy to navigate, and would use it in the future. Since its inception in March 2021, this web application has been accessed by over 26,554 new users from across Florida and beyond.
Speakers
avatar for Sandra Wilson

Sandra Wilson

Professor, University of Florida
Dr. Sandra Wilson is a Professor of Environmental Horticulture at the University of Florida’s main campus in Gainesville. She received B.S. and M.S. degrees from the University of Delaware and a Ph.D. in Plant Physiology from Clemson University. Dr. Wilson completed postdoctoral... Read More →
Co-authors
HK

Heather Kalaman

UF IFAS Extension Orange County
RM

Rachel Mallinger

University of Florida
NA
Wednesday September 25, 2024 10:00am - 10:15am HST
Coral 1

10:00am HST

PH 2 - Postharvest Performance of Tomato Fruit at Shelf-life Conditions in Relation to Fertilization Rate During Plant Producti
Wednesday September 25, 2024 10:00am - 10:15am HST
This study investigates how the ripening stage at harvest and fertilization rate impact tomato fruit quality. Tomato plants (cv. HM1823) were grown during the Fall 2023 season under four levels of fertilization (75%, 100%, 125%, and 150% of the recommended UF/IFAS rate, which is 200 lb/A nitrogen and potassium). Fruit were harvested at four distinct ripening stages: mature green (MG), turning (T), pink (P), and red (R), as assessed visually and stored at 25°C. Color, firmness, soluble solids content (SSC), pH, and titratable acidity (TA) were measured every 4 days until 12 or 16 days (for MG fruit). According to the analysis of variance, all factors (fertilization rate-FR, ripening stage at harvest- RS and shelf life period- SL), as well as the interaction RSSL significantly (P
Speakers
ST

Sotirios Tasioulas

SWFREC - University of Florida/IFAS
Co-authors
JW

Jessie Watson

SWFREC - University of Florida/IFAS
NA
PT

Pavlos Tsouvaltzis

Southwest Florida Research and Education Center, University of Florida
NA
Wednesday September 25, 2024 10:00am - 10:15am HST
Nautilus

10:00am HST

VSF 2 - Diversity and Abundance of Bees Visiting Grape Flowers in Wisconsin Vineyards
Wednesday September 25, 2024 10:00am - 10:15am HST
Bees play an essential role in plant pollination and the ecosystem services they provide to increase the quantity and quality of many agricultural crops. Many food crops are either dependent on, or benefit from, bee pollination, yet bees have experienced population declines due to a combination of factors including pesticide exposure, susceptibility to pathogens and parasites, habitat loss through land use intensification, and lack of suitable resources. Most studies on pollinator conservation and pollination services focus on pollinator-dependent crops and fail to address the role self- and wind-pollinated plants, such as grapes, play in maintaining or threatening pollinators. In vineyards, bees are often overlooked, as they are not required for the pollination of grape. This study aimed to survey the bees visiting grape flowers of cold climate grapes by recording the abundance and diversity of bees. Observations were conducted at six commercial vineyards in South Central Wisconsin during grape bloom, late May to mid-June in 2022. At each vineyard, vines of mixed cold-climate grape varieties were observed between 10:00 and 17:00 hrs on six separate days. Timed observations (5 min per vine) of bees visiting grape inflorescences were conducted at ten randomly selected grape plants per plot per day. In addition, each day, one 10 min wandering transect was walked along two adjacent rows of grape vines and all bees observed visiting grape inflorescences were recorded. To prevent destructive sampling, bees were identified in the field to species when known or assigned to one of 14 pre-determined morphospecies representing 24 likely genera. Timed plant observations showed a total of 1,059 floral visitations, with an average of 0.31 /- 0.16 bees per minute of sampling. During the wandering transects, a total of 417 floral visitations were observed, with 1.44 /- 0.43 bee visits per minute of sampling. This research underscores the need for reduced pesticide inputs, particularly around bloom time, to protect the diversity and abundance of bees visiting grape inflorescences.
Speakers
CG

Christelle Guedot

University of Wisconsin - Madison
Co-authors
ML

Mitchell Lannan

University of Wisconsin - Madison
NA
Wednesday September 25, 2024 10:00am - 10:15am HST
Kahili

10:00am HST

Organic Horticulture Interest Group - Discussion on the regional activities of USDA-AMS Transition to Organic Partnership Program (TOPP)
Wednesday September 25, 2024 10:00am - 11:00am HST
Discussion on the regional activities of USDA-AMS Transition to Organic Partnership Program (TOPP)
Speakers
avatar for Christine Coker

Christine Coker

Professor or Urban Horticulture, Mississippi State University
Wednesday September 25, 2024 10:00am - 11:00am HST
Sea Pearl 4

10:00am HST

Wellness Station
Wednesday September 25, 2024 10:00am - 4:00pm HST
Complimentary Seated Massage - First-Come, First-Served
Wednesday September 25, 2024 10:00am - 4:00pm HST
Coral 4/5 - Conference Central

10:14am HST

Herbs, Spices, and Medicinal Plants 1 (HSMP 1)
Wednesday September 25, 2024 10:14am - 11:45am HST
Saffron (Crocus sativus) Production in North Central New Mexico - Saeid Zehtab Salmasi
Experiences Cropping Wasabi in Inland Oregon - Clint Taylor
Double-season production of hops (Humulus lupulus L.) with photoperiod manipulation in a subtropical climate - Shinsuke Agehara
Brewing Conditions and UV treatment affect Shelf Life of Cold-brew US-grown Tea - Yan Chen
Withering Duration and Drying Temperature Significantly Affect Postharvest Quality of Kratom (Mitragyna speciosa) - Mengzi Zhang
Consumer preference of saffron uses in baked goods and dishes - Hideka Kobayashi
Moderator
SZ

Saeid Zehtab Salmasi

Associate Professor and Research Director, New Mexico State University
Research Director of the Sustainable Agriculture Science Center of New Mexico State University in Alcalde
Wednesday September 25, 2024 10:14am - 11:45am HST
South Pacific 1

10:14am HST

Water Utilization and Management 1 (WUM 1)
Wednesday September 25, 2024 10:14am - 11:45am HST
Developing Irrigation Tools and Information for Effective Irrigation Management of California’s Avocado Orchards - Ali Montazar
Comparative Assessment of Satellite-Derived Crop Evapotranspiration and Estimated Evapotranspiration in Almond and Walnut Orchards for Precision Irrigation Management - Abdelmoneim Mohamed
Physiological Thresholds Provide Targets for Climate-Resilient Irrigation Management in Hazelnut - Steven Bristow
Determination of Crop Coefficient and its Inter-annual Variability in Pecan Orchard in Georgia - Kriti Poudel
Assessing Water Status in Citrus Plants Using Thermal Imaging in Greenhouses - Gustavo Haddad Souza Vieira
The Impact of Irrigation Rates Based on Crop Water Requirement on Tree Growth and Water Relations in Commercial Citrus Groves - Alisheikh Atta
Moderator
AM

Ali Montazar

University Of California Cooperative Extension
Wednesday September 25, 2024 10:14am - 11:45am HST
South Pacific 4

10:15am HST

CHMG 1 - Invasive Potential of Ornamental Landscape Plants in Southern Arizona
Wednesday September 25, 2024 10:15am - 10:30am HST
Plants introduced through ornamental horticulture have contributed to exotic species invasions around the world. Fifteen landscape plants commonly used in horticulture in southern Arizona have been evaluated using the Plant Risk Evaluator (PRE) tool developed by PlantRight and Cal-IPC. The evaluations have recognized eight of these species as presenting high potential risk of escaping cultivation and becoming invasive in Arizona. The process of evaluating ornamentals has fostered communication and collaboration between invasive species managers and Extension horticulture agents, who previously had little interaction. The findings have been communicated through several articles on invasive species in the trade magazine, Southwest Horticulture. This information has contributed to two cultivated species (Searsia lancea and Ulmus pumila) and the entire genus Tamarix being newly added to the Arizona State Noxious Weed List. Arizona Master Gardeners have been educated on invasive plant issues to foster outreach to the public and to aid their planting choices.
Speakers
MC

Michael Chamberland

University of Arizona
Wednesday September 25, 2024 10:15am - 10:30am HST
South Pacific 2

10:15am HST

HSMP 1 - Saffron (Crocus sativus) Production in North Central New Mexico
Wednesday September 25, 2024 10:15am - 10:30am HST
Saffron (Crocus sativus L.) holds a high economic value as the world's most expensive spice. It plays a crucial role in many small farm economies in countries such as Iran, India, Afghanistan, Greece, Morocco, Spain, and Italy. In this project the effects of three different corm size (10 ,9 , and 8 ) on saffron stigma’s yield were studied in hoop house and open field production systems in a randomized complete block design with three replications at Certified Organic Farm of the Sustainable Agriculture Science Center of New Mexico State University in Alcalde located in north central New Mexico. Saffron corms were planted 6 to 8 inches in depth and 6 inches apart on September 19, 2023. The saffron flowers were harvested from October 18th until November 6th, 2023, in different treatments. Flowering started earlier and the flowering period was longer in plots planted with bigger corms. Saffron flowers picked up in the morning and then the stigmas were separated and dehydrated at 100 °C for ten minutes. The first-year results showed that the highest number of flowers were obtained from 10 corm size plots. The highest yield of dehydrated stigmas was also harvested from 10 corm size in high tunnel production system with 3.43 Kg/ha (3.06 lbs./acre). The bigger corms of saffron produced significantly higher stigma yield than smaller ones; 2.84 kg/ha (2.53 lbs./acre) for 10 corms vs 0.89 kg/ha (0.79 lbs./acre) for 8 corms, however, the difference between saffron yield in high tunnel and open field production systems was not significant. In summary the outcome of this research shows that saffron can be produced successfully even in open field production system in northern New Mexico and using bigger corms for planting may produce higher saffron in the first year, although the evaluation of winter hardiness and the following years performance will be necessary.
Speakers
SZ

Saeid Zehtab Salmasi

Associate Professor and Research Director, New Mexico State University
Research Director of the Sustainable Agriculture Science Center of New Mexico State University in Alcalde
Co-authors
RH

Robert Heyduck

New Mexico State University
NA
Wednesday September 25, 2024 10:15am - 10:30am HST
South Pacific 1

10:15am HST

NUTS 1 - Enhancing Pecan Tree Resilience Against Spring Freeze Events: Insights from Secondary Bud Dynamics and Carbohydrate Analysis
Wednesday September 25, 2024 10:15am - 10:30am HST
Pecan growers face significant challenges in protecting their crops from the spring freezes that devastate yields. Conventional freeze protection methods are impractical for the large, tall trees. This study investigated the dynamics of the secondary bud break in pecan trees, a mechanism that ensures yield when primary buds are damaged. In three pecan cultivars ('Pawnee,' 'Kanza,' and 'Maramec') we characterized the sprouting potential of secondary buds after the primary buds were cold damaged. Primary bud shoots were collected at three different bud growth stages: outer bud scale shed stage, inner bud scale shed stage, and early bloom stage. The shoot samples held at 6°C to terminate primary buds’ growth. The cold treated shoots were then held in growth chambers set to mimic spring humidity, temperature, and light conditions. The percentage of branches with secondary bud break was recorded. The carbohydrate levels (sugar and starch) were measured in the apical shoots treated at the outer bud scale shed stage. The results showed variation among cultivars in the number of shoots with secondary buds and the stage at which primary buds were terminated. The ‘Kanza’ and ‘Pawnee’ cultivars produced more secondary buds when the primary buds were cold damaged in the outer bud scale shed stage. The ‘Maramec’ cultivar produced more secondary buds when the primary buds were cold damaged in the inner bud scale shed stage. The A carbohydrate analysis of the three cultivars demonstrated a correlation between successful secondary bud break and elevated carbohydrate levels in the one-year-old shoots. Cultivars with higher secondary bud break rates, 'Kanza' and 'Pawnee', had higher carbohydrate levels than 'Maramec.' These findings suggest that higher carbohydrate levels in one-year-old shoots facilitate successful secondary bud break following spring freeze damage to primary buds. This research suggests developing production practices to improve tree carbohydrate levels in the late summer and fall could potentially protect pecan production from spring freeze damage.
Speakers
LZ

Lu Zhang

Oklahoma State University
Co-authors
AK

Amandeep Kaur

Oklahoma State University
NA
JM

Justin Moss

Oklahoma State University
NA
LF

Louise Ferguson

University of California, Davis
NA
NM

Niels Maness

Oklahoma State University
NA
YS

Yanwei Sun

Oklahoma State University
NA
Wednesday September 25, 2024 10:15am - 10:30am HST
Lehua Suite

10:15am HST

O/LT 1 - Pesticide Management Decisions Affect Contamination of Nectar in Containerized Ornamental Plant Production
Wednesday September 25, 2024 10:15am - 10:30am HST
Declines in pollinator populations have gained much attention over the last decade. Exposures to pesticides are one potential contributor to these declines. Given that the ornamental plant production industry produces crops that are attractive to pollinators and that pesticide use is often integral to ensuring plants are pest-free, attention is needed to assess and possibly reduce contamination of flower nectar and pollen before plants go to market. Three major factors associated with pesticide management practices that may influence contamination of floral resources are: application method, application rate, and application timing relative to flowering. Using the systemic insecticide thiamethoxam as a model pesticide and Salvia x ‘Indigo Spires’ (Salvia longispicata x S. farinacea) as a model species, this study investigated the influence of each of these factors on contamination of nectar. Plants were treated by spray and drench methods, at low and high rates according to the pesticide label, and before flower buds formed or close to the time of floret opening. Nectar samples were collected using microcapillary tubes when all plants were uniformly flowering and thiamethoxam concentrations were analyzed by LC-MS/MS. Concentrations of thiamethoxam in nectar were highest in drench applications, regardless of application timing and rate, and exceeded published LC50s for native bees and/or honeybees. Thiamethoxam concentrations were much lower in the spray-applied treatments, but they still exceeded published LC50s for native bees and/or honeybees except for the spray treatment applied before blooming at the low rate. These results provide insight into how some pesticide management practices influence contamination of floral resources and indicate a need for developing best management practices focused on limiting thiamethoxam exposures once plants go to market. Additional studies are underway to evaluate other plant species and systemic insecticides to address gaps in knowledge.
Speakers
avatar for Patrick Wilson

Patrick Wilson

University of Florida
pesticide fate and toxicology, pollinator protection, pesticide analysis in different matrices
Co-authors
MC

Mia Cabrera

university of florida
NA
SW

sandra wilson

University of Florida
Dr. Sandra Wilson is a Professor of Environmental Horticulture at the University of Florida’s main campus in Gainesville. She received B.S. and M.S. degrees from the University of Delaware and a Ph.D. in Plant Physiology from Clemson University. Dr. Wilson completed postdoctoral... Read More →
VR

Vanesa Rostan

university of florida
NA
Wednesday September 25, 2024 10:15am - 10:30am HST
Coral 1

10:15am HST

PH 2 - Identification of NAC Transcription Factors Involved in the Ripening and Senescence of Pepper (Capsicum annuum L.) Fruit
Wednesday September 25, 2024 10:15am - 10:30am HST
Pepper (Capsicum annuum L.) as a non-climacteric fruit is usually harvested at either mature green or ripe stage, and fruit quality including texture, color, and nutritive values is different according to the fruit developmental stages. Understanding molecular mechanisms of fruit ripening and senescence processes is crucial to control the fruit quality and reduce the postharvest loss. This study was aimed to identify NAC transcription factors (TFs) involved in the ripening and senescence of pepper fruit by analyzing gene expressions and protein-protein interactions. To identify NAC genes related to the ripening, transcript levels of total 104 NAC TFs were investigated using publicly available transcriptome data. Among them, transcript levels of 8 genes significantly increased during the ripening and senescence. Their phylogenetic analysis also showed that they are closely associated with NAC TFs which play a role in ripening or senescence in other crop species. Among them, 4 genes showing abundant transcript levels at the ripening stage were selected, including CaNAC14, 45, 84, and 92, to further investigate their roles. In pepper (C. annuum var. ‘Gyeonggiyangpyeiong’) fruit, their expressions clearly increased during the ripening or induced by exogenous ABA, the major phytohormone regulating non-climacteric fruit ripening. To reveal a relationship between the CaNACs and ABA signaling, protein-protein interactions between the CaNACs and ABA receptors were analyzed through yeast- two-hybrid and bimolecular-fluorescence complementation assays. Among them, CaNAC92 and CaPYL12, the ABA receptor, interacted each other in a nucleus with an ABA-dependent manner. This result indicated that their interaction may affect the ABA signaling or the transcriptional regulation during the ripening. We identified CaNAC TFs involved in the pepper fruit ripening and suggested CaNAC92 as a putative molecule functioning in the ripening. These findings will provide genetic information to control the ripening and to improve pepper fruit quality.
Speakers
KM

Kyeonglim Min

Student, Seoul National University
Co-authors
EJ

Eun Jin Lee

Seoul National University
Wednesday September 25, 2024 10:15am - 10:30am HST
Nautilus

10:15am HST

VSF 2 - The Grape Health Index: Validation of a New Methodology for Quantifying Wine Grape Spoilage by Means of FT-MIR Spectrosc
Wednesday September 25, 2024 10:15am - 10:30am HST
For wineries processing hand harvested grapes, a visual inspection of microbial spoilage is expedient and cost effective. However, due to the increasing adoption of machine harvesters, which frequently rupture berry skins making visual inspection less precise, and the high degree of error of visual inspections between individuals, a quantitative approach to assess spoilage is needed. Fourier Transform Mid-Infrared Spectroscopy (FT-MIR), combined with multivariate analysis, is being investigated as an approach to predict grape health as a sample that can be analyzed in less than one minute. Calibration data was obtained from grape samples of Chardonnay, Riesling, Petite Sirah, and Zinfandel, which were sorted into fractions of 0, 5, 10, 15, and 20% microbially impacted clusters in healthy grape material, and analyzed using the spectrophotometer. The spectral data was analyzed using the Partial Least Squares (PLS) regression. Predicting factors included volatile acidity (VA), gluconic acid, ethanol, lactic acid, glucose-fructose content, Brix, titratable acidity, tartaric acid, malic acid, pH, alpha amino nitrogen, ammonia, and yeast assimilable nitrogen (YAN). A model was selected which optimized for a high coefficient of determination (R2) and a low root mean squared error (RMSE). Additional selection criteria included the extent to which predicting factors have been observed to correlate with microbial spoilage in other studies. The model selected had a R2 of 0.620 and a RMSE of 4.596, making it suitable for identifying grapes marked by spoilage. The output of the model was converted to Grape Health Index (GHI) Scores for better usability by operators at the test stand. The GHI was implemented at the test stands of two large-scale wineries during the 2022 and 2023 seasons. Additional wineries will be added in 2024. The testing of the impact of microbial spoilage on wine aging is currently underway. Wines made in 2018, 2019 and 2020 are evaluated for color degradation and browning, anthocyanins, tannins, pigments, and acetaldehyde. The results will be compared with the original harvest results for comparative analysis concluding the project.
Speakers
SV

Sonet Van Zyl

Fresno State
Co-authors
SS

Stephan Sommer

University of Missouri
NA
SC

Steven Craig Ebersole

Foley Family Wines
NA
Wednesday September 25, 2024 10:15am - 10:30am HST
Kahili

10:15am HST

WUM 1 - Developing Irrigation Tools and Information for Effective Irrigation Management of California’s Avocado Orchards
Wednesday September 25, 2024 10:15am - 10:30am HST
In California, avocado is primarily grown in Southern and Central parts of the state, typically in regions tempered by coastal climates and fine or course sandy loam soils. These regions face uncertain water supplies, mandatory reductions of water use, and the rising cost of water, while efficient use of irrigation water is one of the highest conservation priorities. Moreover, due to increasing salinity in water sources, effective irrigation is more critical to ensure optimal yield and high-quality avocado fruits. A two-year study was conducted in 12 mature avocado sites in California. Extensive field measurements and surveys were conducted to better understand the current water management practices, to acquire and develop relevant information on crop water use (ET) and crop coefficients, and to assess the performance of satellite-based OpenET tool for irrigation management in avocados. Surface renewal and eddy covariance equipment was used to measure actual evapotranspiration (ETa) in each site. The results illustrated considerable variability in avocado crop water consumption both spatially and temporally. The crop coefficients curves were developed for each site. Across the avocado research sites, the average seasonal crop coefficient values varied from 0.6 to 0.76. The findings demonstrated that canopy features, soil types and conditions, pruning practices, soil surface cover, and row orientations need to be considered to perform effective water management in avocado orchards. Ground shading percentage and row orientations provide a good estimation of canopy size/volume and the amount of light that it can intercept are likely the most important drivers influence crop water needs. The RMSE of the measured ETa from eddy covariance equipment and estimated ETa from Ensemble OpenET varied from 0.53 to 1.37 mm d-1. The preliminarily findings indicated that the Ensemble OpenET estimates ETa relatively well in some sites and could be an effective irrigation management tool in the future for avocado orchards, however more evaluations are required.
Speakers
AM

Ali Montazar

University Of California Cooperative Extension
Wednesday September 25, 2024 10:15am - 10:30am HST
South Pacific 4

10:15am HST

Workshop: Breeding for Post-harvest Shelf Life in Vegetable Crops
Wednesday September 25, 2024 10:15am - 12:15pm HST
Improved postharvest storage is a major target for vegetable-crop production. Nutritional contents and flavor are traits highly affected from harvesting to final consumption. Several reports have demonstrated that these traits are strong driving factors impacting consumers’ decisions and preferences when purchasing food. Therefore, these are economically important traits related to strong commercialization potential and improved market value. Assessing the potential variation in post-harvest shelf life among vegetable crops will be critical to identify parental lines that can be used in breeding programs to improve these traits and their effect on the quality of produce. Additionally, investigating the genetic mechanisms underlying post-harvest shelf life in horticultural crops is critical. This will result in the development of molecular breeding tools associated with improving shelf life, which will accelerate breeding. This interest group seeks to provide case studies from experts worldwide who have worked on breeding for nutritional contents, flavor, and post-harvest shelf life in vegetables.

Coordinator(s)
  • Carlos Avila, Texas A&M AgriLife Research, Weslaco, TX, United States
Moderator(s)
  • Dennis Nicuh Lozada, New Mexico State University, Plant and Environmental Sciences, Las Cruces, NM, United States
  • Devi Kandel, Langston University, United States
Speaker/Participant(s)
  • Carmit Ziv, Institute of Postharvest and Food Science, ARO, Volcani Institute, Israel
    Breeding for improved postharvest quality of peppers during cold storage (15 mins)
    Summary: Dr. Ziv's research program studies postharvest disease control of fruity vegetables by developing environment-friendly treatments to control postharvest fungal rot, understanding resistance mechanisms of phytopathogenic fungi to cold storage, uncovering the role of lipids in determining the interactions between fruits and pathogenic fungi during storage.
  • Carlos Avila, Texas A&M AgriLife Research, Weslaco, TX, United States
    Lack of locule gel increases post-harvest shelf-life and quality in tomato (15 mins)
    Summary: Dr. Avila's research program is focused on the development of tomato and spinach cultivars adapted to Texas conditions. Research work includes the characterization of plant a/biotic stress and vegetable quality trait regulation using molecular, physiological, and biochemical approaches to develop applied strategies to improve breeding efficiency and ultimately crop resistance and quality. Current efforts include the development of firm long shelf-life tomato cultivars with improved flavor and nutritional content.
  • Bhimu Patil, Texas A&M University, Vegetable and Fruit Improvement Center, College Station, Texas, United States
    Post-harvest shelf life and nutritional quality in horticultural crops (15 mins)
    Summary: Dr. Patil has been actively involved in both research and educational activities related to global level ‘Foods for Health’. His research, in collaboration with trans-disciplinary scientists, related to bioactive-derived assays led to isolation and characterization of certain bioactive compounds in citrus and turmeric and other vegetables. His research is also focused on pre and postharvest effects on bioactive compounds in citrus, onion and recently his work is focused on other vegetables and fruits.
  • Alexander Goldshmidt, Institute of Plant Sciences, ARO, Volcani Institute, Israel
    Exploring the Role of CLASS-II KNOX mediated Ripening Pathways for the Discovery of Novel Genetic Approaches to Extend Fruit Shelf Life (15 mins)
    Summary: Dr. Goldshmidt research is focused on investigation of the genetic factors and networks controlling development of the pericarp tissues in fruits of Solanaceae crops tomato and pepper. His lab seek to use their research to develop novel genetic solutions and tools to extend fruit's shelf life and improve its consumer attractiveness and nutritional values.
  • Isabel Ortega-Salazar, UC Davis, United States
    Control of fruit ripening, improvement of fruit quality, and fruit-pathogen interactions. (15 mins)
    Summary: Dr. Blanco-Ulate's research program integrate systems biology approaches with biochemical and physiological analyses to study fruit development and quality traits, and to establish a novel framework for the early detection and efficient management of fruit diseases.





Wednesday September 25, 2024 10:15am - 12:15pm HST
Coral 2

10:29am HST

Nursery Crops/Root Growth and Rhizosphere Dynamics (NUR/RHIZ)
Wednesday September 25, 2024 10:29am - 11:00am HST
A Snapshot of Nursery Container Substrate Prices in Tennessee - Amy Fulcher
Suppression of Stem Elongation of UV-B Treatment Timing and Intensity on Tomato Scion and Rootstock in Plant Factory with Artificial Lighting - Dongcheol Jjang
Effects of Nitrogen on the Nodulation of Ceanothus velutinus - PRAKRITI NEPAL

Moderator
PN

PRAKRITI NEPAL

Utah State Univeristy
Wednesday September 25, 2024 10:29am - 11:00am HST
South Pacific 3

10:30am HST

NUR/RHIZ - A Snapshot of Nursery Container Substrate Prices in Tennessee
Wednesday September 25, 2024 10:30am - 10:40am HST
In container nursery production, the potting substrate serves as the foundation of the crop, providing essential nutrients, aeration, and moisture retention. A well-chosen substrate is crucial for optimizing plant health and growth, and ultimately influences a nursery’s economic viability since substrate is estimated to be 13% of the total variable costs of 3-gallon production. Our objective is to determine the cost of common potting substrate components. The study goal is to identify opportunities for nursery producers to reduce costs and increase profits by optimizing their substrate components. Email surveys supplemented by interviews were used to collect data from six Tennessee container nursery producers. All growers were within a 117-mile radius of McMinnville, Tennessee, and provided the prices they paid in the last 12 months for the primary components of their potting substrate. Delivery fees and fuel surcharges were not considered in the cost when these expenses were itemized. However, one producer’s prices included delivery expenses. Participating nursery producers self-identified as having 100 (66.7%) acres in container production. Growers in this case study paid an average of $19.07 (SD=$5.53) per yard3 for pine bark; however, the prices ranged from $14.53 to $29.00 per yard3. In general, smaller producers paid more for pine bark. Sand ranged from $40.50 to $49.95 per yd3. Nursery producers paid $1.12 to 1.32 per pound for controlled release fertilizer (CRF), an input estimated at 5% of total variable costs. Therefore, some growers paid 18% more for CRF than others. Growers paid from $0.01 to $0.11 per pound for lime. Tennessee nursery producers were paying on average $66.79 per cubic yard for wood fiber-based substrate and vermicompost, 250% more than the average cost of pine bark but just 46% of the market price for peat. These preliminary data show 1) individual nurseries pay a range of prices for the same potting substrate components, and 2) the relatively high cost of peat replacements, i.e., wood fiber and vermicompost. The data highlight the range of prices being paid for CRF and its relative expense, underscoring the opportunity for growers to reduce costs by optimizing their use and placement of CRF. Additional research is needed to more broadly sample nurseries for these data and determine the influence of nursery size on substrate component costs. cparwutcakwt8uvpamtb
Speakers
AF

Amy Fulcher

University of Tennessee
Co-authors
AR

Alicia Rihn

University of Tennessee
JO

James Owen

USDA-ARS
Jim Owens is a USDA-ARS Research Horticulturist located at the Application Technology Research Unit in Wooster, OH. Jim received his B.Sc. in Plant and Soil Science at the University of Kentucky, his M.Sc. in Environmental Science at the University of Rhode Island, and Ph.D. at North... Read More →
WH

Walker Harrell

University of Tennessee
NA
Wednesday September 25, 2024 10:30am - 10:40am HST
South Pacific 3

10:30am HST

CHMG 1 - Education on Water Saving Landscaping Practices Results in Measurable Water Conservation
Wednesday September 25, 2024 10:30am - 10:45am HST
Florida is facing critical water scarcity due to population growth. The home landscape is an opportune setting to make changes in practices to conserve water. Extension programs on water conservation in the landscape can provide residents with the information they need to adopt new practices to conserve water. In 2021-2023, UF/IFAS Extension residential horticulture programs in Central Florida, educated 6,474 residents about sustainable landscape practices. Based on client self-reported follow-up surveys (n=342), the annual water savings due to adoption of best landscaping practices was approximately 11.6 million gallons. It saved homeowners $49,977 in utility bills (based on regional value of $4.30 per 1,000 gallons). The most adopted practices included calibrating sprinkler systems to deliver ½ inch to ¾ inch of water instead of 1 inch, reducing irrigation frequency during summer, and reducing irrigation frequency during winter. Adoption is lower for water conservation practices that require installing soil moisture sensors and converting or installing irrigation spray heads. Participants reported (n=342): 35% eliminated irrigation or converted to low volume irrigation, 46% reduced irrigation from 3 to 2 days per week, 45% reduced irrigation frequency in winter, 46% reduced irrigation frequency in summer, 25% using a working rain shutoff device, 37% calibrated irrigation to ½” or ¾” rather than 1” or more. Impacts of changes in household water use multiply when life cycle assessment of a water supply system is considered. The reduction of 11.6 million gallons of residential water use resulted in saving 40,022 kWh of energy and $30,162 in treating and delivering water to the end users, reducing 28 metric tons of carbon dioxide equivalent of greenhouse gas emissions, deferring the need for $98,734 investment in alternative water supply infrastructure, and potentially reducing stormwater runoff and avoiding water quality deterioration. The impacts of these water conservation efforts are even greater than the values represented here based on total program attendance. This shows the efforts of extension agents educational programs resulted in measurable water savings to help Florida households conserve water.
Speakers
TS

Tia Silvasy

University of Florida
Wednesday September 25, 2024 10:30am - 10:45am HST
South Pacific 2

10:30am HST

HSMP 1 - Experiences Cropping Wasabi in Inland Oregon
Wednesday September 25, 2024 10:30am - 10:45am HST
Wasabi (Eutrema japonicum) is a high-value and notoriously difficult crop traditionally cultivated in East Asia. There has been some limited commercial cultivation of the crop in the Pacific Northwest, including in the coastal mountains of Oregon. This presentation shares insights into the challenges and approaches to inland wasabi farming, focusing on environmental conditions, potential growing systems, and media types. Key points include: 1. Environmental Conditions: Wasabi has a narrow range of light, temperature, and water requirements and is prone to a myriad of fungal and bacterial diseases. Knowing this narrow range is essential to the practicality of commercial wasabi production. Balancing the ideal environmental factors for wasabi without creating disease outbreaks is a primary challenge in wasabi production. 2. Growing System
Speakers
avatar for Clint Taylor

Clint Taylor

Oregon State University - NWREC
Co-authors
CB

Chip Bubl

Oregon State University
NA
CO

Cynthia Ocamb

Oregon State University
NA
DM

Dalyn McCauley

Oregon State University
LN

Lloyd Nackley

Oregon State University
Lloyd Nackley is a plant ecologist who applies a systems approach to improve nursery and greenhouse management. Nackley's research program at Oregon State University focuses on addressing four challenges facing nursery and greenhouse production in Oregon: irrigation application, pest... Read More →
Wednesday September 25, 2024 10:30am - 10:45am HST
South Pacific 1

10:30am HST

NUTS 1 - Biochar in Pecan Orchards: Unraveling Water Stress Dynamics for Sustainable Irrigation Management
Wednesday September 25, 2024 10:30am - 10:45am HST
Pecan orchards in the southwestern United States face significant challenges due to persistent drought conditions that adversely affect yield and nut quality. Pecans are recognized as the most water-intensive crop in the region, and therefore require innovative strategies to optimize the available irrigation water. This study explores the use of pecan wood-derived biochar as a soil amendment to enhance the soil water-holding capacity and alleviate water stress in pecan orchards. We conducted field experiments during the summer of 2023 in a flood-irrigated pecan orchard located in the Mesilla Valley, New Mexico, USA. We accounted for the irrigation gradient in a flood-irrigated orchard by using a randomized complete block design with four blocks, each containing three equidistant trees from the irrigation valves. The trees within each block were randomly assigned to one of three biochar application rates: 0 kg/ha (treated area), 6300 kg/ha, and 12600 kg/ha. The treated area was situated within the herbicide strip, spanning 9.14 m in length – centered on the tree – with 1.22 m on either side of the tree. To assess tree water status, two leaf samples from the lower shaded canopy were covered in aluminum foil bags for a minimum of 15 minutes before mid-day stem water potential was measured. Mid-day stem water potential was measured throughout the growing season near the end of each irrigation dry-down cycle. For each tree, we calculated the average mid-day stem water potential and then performed an ANOVA to compare the averages across the treatment groups. In the five months after biochar application, there were no significant differences in tree water status across the treatment groups. This highlights the need for more research to study the interactions among soil moisture content, biochar amendment applications, and pecan tree mid-day stem water potential. This study contributes to the ongoing discourse that calls for enhancing crop water use efficiency in arid regions by providing a foundation for future studies that seek to use biochar as a sustainable agricultural practice in pecan orchards.
Speakers
JM

Jamin Miller

New Mexico State University
Co-authors
AF

Alexander Fernald

New Mexico State University
NA
CV

Ciro Velasco-Cruz

New Mexico State University
NA
DD

David Dubois

New Mexico State University
NA
JI

John Idowu

New Mexico State University
NA
RH

Richard Heerema

New Mexico State University
NA
Wednesday September 25, 2024 10:30am - 10:45am HST
Lehua Suite

10:30am HST

O/LT 1 - Comparing Pollinator Species Richness and Abundance Between Pycnanthemum Species and Accessions
Wednesday September 25, 2024 10:30am - 10:45am HST
Pollinators play a crucial role in the ecosystem, human health, and the economy. However, despite the significance of pollinators, their populations are declining globally. Pycnanthemum is a marketable pollinator-attractive plant that could supplement pollinator resources in the landscape. Breeders would benefit from a comparison of the pollinator attractiveness between Pycnanthemum species and accessions. Cultivating Pycnanthemum should focus on aesthetic traits and maximizing pollinator abundance and species richness. Pollinator visitation was compared among three species and five accessions of Pycnanthemum (P. flexuosum (F), P. virginianum (V), and three accessions of P. tenuifolium (T1-T3)) using observations and capture. Lepidoptera, honey bees (Apis mellifera), Diptera, carpenter bees (Xylocopa spp.), small bees, and bumble bees (Bombus spp.) were observed most abundantly on Plant F. Plant V attracted the highest number of pollinators overall, with Apis mellifera (honey bees) accounting for more than half of the pollinator visitation. Xylocopa spp. (carpenter bees) and honey bees did not have a significant preference between the species. Plants F, T2, T3, and V attracted the greatest abundance of Diptera (flies). Wasps were most attracted to Plants T3 and V, while Bombus spp. (bumble bees) was observed most often on Plants F and V. Plant F attracted the highest number of Lepidoptera (butterflies and moths) and small bees. The species richness of pollinators did not significantly differ across Pycnanthemum species, with at least 24 to 29 different pollinator species visiting each plant. A range of factors, including olfactory cues, the morphology of plants, and accessibility of resources, may have affected pollinator preferences. Determining which Pycnanthemum species attracted an abundance and diversity of pollinators provides breeders a foundation for cultivation and conservation expectations.
Speakers
KS

Kaitlin Swiantek

Ball FloraPlant
Co-authors
JR

John Ruter

University of Georgia
Allan Armitage Endowed Professor of Horticulture and Director, Trial Gardens at UGA
Wednesday September 25, 2024 10:30am - 10:45am HST
Coral 1

10:30am HST

PH 2 - Comparative Analysis of Volatile Organic Compounds of Eight Microgreens in the Brassicaceae Family
Wednesday September 25, 2024 10:30am - 10:45am HST
This study investigates the volatile organic compounds (VOCs) of eight microgreens in the Brassicaceae family, known for their robust flavors and potential health benefits, including detoxification properties and anti-cancer effects. The microgreens analyzed included five Brassica species—B. juncea (mustard), B. napus (kale), B. rapa (mizuna), B. oleracea L. var. capitata (red cabbage), and B. oleracea L. var. italica (broccoli)—as well as Eruca sativa (arugula), Lepidium sativum (cress), and Raphanus sativus (radish). The above-ground plant tissues were homogenized in saturated salt water and the VOCs were examined using headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GCMS). A total of 117 VOCs were identified across all tested species, with individual species VOC counts ranging from 42 to 67. Radish showed the highest total ion current at 676 million (mTIC), with other species varying down to 190 mTIC in arugula. Notably, sulfur and/or nitrogen-containing compounds (SCCs and/or NCCs) constituted over 90% of the total VOCs collected from all species, with Brassica species containing more than 96% SCCs and 93% NCCs. Conversely, radish and arugula had less than 88% NCCs, and cress contained only 66% SCCs. Dominant compounds were primarily isothiocyanates, with significant variability in major VOCs across different species. For instance, allyl isothiocyanate was prevalent in mustard, mizuna, and red cabbage; 4-isothiocyanato-1-butene in kale and mizuna; isothiocyanatomethyl-benzene in cress; 1-isothiocyanato-4-(methylthio)-butane in arugula and broccoli; and (E)-4-isothiocyanato-1-(methylthio)-1-butene in radish. The reduced SCCs in cress were attributed to the higher presence of benzyl nitrile and benzyl isocyanate, while the lower NCCs in radish were linked to increased levels of dimethyl disulfide and dimethyl trisulfide. This diversity in SCCs and NCCs contributes to the varied volatile flavors and potential health benefits of these microgreens.
Speakers
avatar for Jinhe Bai

Jinhe Bai

Research Chemist, USDA-ARS
preserve the postharvest flavor and nutrient quality of fruits and vegetables. Specific areas of expertise include controlled-atmosphere storage, modified atmosphere packaging, and edible coating technologies, and the discovery of how internal and environmental factors influence metabolism... Read More →
Co-authors
AP

Anne Plotto

USDA-ARS
NA
ER

Erin Rosskopf

USDA-ARS
NA
FD

Francesco Di Gioia

Pennsylvania State University
JH

Jason Hong

USDA Agricultural Research Service
NA
LW

Libin Wang

Nanjing Agricultural University,
NA
WZ

Wei Zhao

USDA-ARS
NA
Wednesday September 25, 2024 10:30am - 10:45am HST
Nautilus

10:30am HST

VSF 2 - World Grape Rootstocks Pedigree Review
Wednesday September 25, 2024 10:30am - 10:45am HST
Grapes belong to the Vitis genus, which includes about 80 species and is considered as two sub-genera, including Euvitis and Muscadinia. Muscadine grapes are sometimes considered as a separate genus in different classification systems and include three species: Muscadinia rotundifolia, Muscadinia munsoniana, and Muscadinia popenoei. The Euvitis sub-genus includes the most commercially important grape varieties. Euvitis is divided into three groups. 1) The American group consists of about 30 species that are important for rootstock breeding; 2) The Asian group consists of about 50 species, which, up to this point, have been underutilized for grape cultivation; and 3) The Eurasian group consists of a single species, Vitis vinifera L., which includes two sub-species of V. vinifera: V. vinifera subsp. sylvestris, considered to be the wild form of grape; and V. vinifera subsp. vinifera, refers to cultivated forms. Of all species in the genus Vitis, the V. vinifera, is undoubtedly the most important worldwide for commercializing grapes and represents the most cultivated varieties worldwide. Other grape species contributed to breeding programs beyond the traditional efforts of rootstock hybridization from wild species. An aphid-like pest, phylloxera (Daktulosphaira vitifoliae), causes damage by feeding on the structural roots of V. vinifera, eventually leading to vine death. In an effort, American Vitis species that coevolved with phylloxera were eventually used as rootstocks for the widely planted V. vinifera scion cultivars. Later, crosses between these wild species became common, and historical breeding efforts led to the modern grape rootstock breeding programs currently active worldwide. Two-thirds of American species have already been used for rootstock breeding. However, it has been reported that the most commonly available rootstocks are derived from just three American species (Vitis berlandieri, Vitis rupestris, and Vitis riparia). Therefore, the most common grape rootstocks have a narrow genetic base, and efforts to extend the gene pools for breeding programs by using other species are of ongoing importance to the industry and scientific community. There are around 1500 grape rootstocks developed in the world, of which around 50 are commonly used as commercial rootstock and play a fundamental role in resistance to biotic and abiotic stresses and adaptation of grapevine to different environmental conditions, a factor that has opened commercial grape growing to regions that might otherwise be overlooked.
Speakers
AR

Alireza Rahemi

Morehead State University
Co-authors
Wednesday September 25, 2024 10:30am - 10:45am HST
Kahili

10:30am HST

WUM 1 - Comparative Assessment of Satellite-Derived Crop Evapotranspiration and Estimated Evapotranspiration in Almond and Walnu
Wednesday September 25, 2024 10:30am - 10:45am HST
Almond and Walnut are the major irrigated crops in the Northern San Joaquin Valley (NSJV) of California. The recurring droughts and climate change in California will likely increase the uncertainty in water supply to almond, walnut, and other specialty crops. Site-specific irrigation is critical to cope with these challenges. Knowing the water consumption of these water use intensive crops is imperative for optimizing irrigation management since it affects nut quality, productivity, and composition. This requires accurate estimates of crop water use (Evapotranspiration, ET). Traditional methods for estimating crop water use are spatially limited, whereas satellite remote sensing of ET offers the advantage of large-scale coverage and is increasingly adopted in irrigated agriculture. This study compares OpenET models, an open-source database providing ET estimates, against calculated ET from weather stations that are commonly used by growers in their irrigation management. Evaluation of OpenET against estimated ET might provide a good opportunity for growers to improve water use efficiency. Such improvements could lead to the adoption of publicly available irrigation management tools and ensure healthier tree development, better resource utilization, and more resilient orchards in the face of climate change. This presentation delves into the preliminary findings of the OpenET evaluation against calculated ET from weather stations in estimating water use for almonds and walnuts, while also examining the potential and challenges associated with each approach for implementation in growers' fields.
Speakers
AM

Abdelmoneim Mohamed

Irrigation and Soils Advisor, University of California ANR
Wednesday September 25, 2024 10:30am - 10:45am HST
South Pacific 4

10:30am HST

Why You Should Be Publishing With ASHS
Wednesday September 25, 2024 10:30am - 11:00am HST
Learn about why you should be publishing the the three open-access, peer-reviewed ASHS journals - JASHS, HortScience, and HortTechnology.
Speakers
avatar for Curt Rom

Curt Rom

University Professor. And Associate Director, University of Arkansas and ASHS
Wednesday September 25, 2024 10:30am - 11:00am HST
Coral 5 - ASHS Hort Theater

10:40am HST

NUR/RHIZ - Suppression of Stem Elongation of UV-B Treatment Timing and Intensity on Tomato Scion and Rootstock in Plant Factory with Artificial Lighting
Wednesday September 25, 2024 10:40am - 10:50am HST
This study aimed to determine the optimal conditions of UV-B exposure for regulating the growth of tomato grafted seedling in Plant Factory with Artificial Lighting by investigating growth characteristics, rhizosphere development, and chlorophyll fluorescence of seedlings. Tomato scion and rootstock were used in the experiment. UV-B treatment timing was divided into three stages from sowing to grafting. UV-B intensities were set at 1.44, 2.88, and 5.76 kJ·m−2·d−1. Results showed that morphology of plants did not exhibit significant differences up to 2.88 kJ·m−2·d−1 for tomato scion and rootstock. However, side effects such as leaf wilting were observed at 5.76 kJ·m−2·d−1. The length of hypocotyl, which is closely related to scion and rootstock suppression of stem elongation, was shortest when treated with 5.76 kJ·m−2·d−1 during the mid-growth stage across all treatment. Interestingly, rhizosphere characteristics such as root volume, surface area, and average root diameter showed improvement trends regardless of treatment timing when exposed to UV-B at 1.44-2.88 kJ·m−2·d−1. Quality indicators of seedlings showed best when 2.88 kJ·m−2·d−1 during the late-growth stage. Comparisons of chlorophyll fluorescence parameters revealed no significant effects of UV-B treatment on cucumber seedlings and grafting except for PIABS. However, tomato seedlings and grafting exhibited significantly reduced FV/FM and DIO/RC at late-growth stage when exposed to 5.76 kJ·m−2·d−1. Therefore, it is concluded that utilizing UV-B in the range of 1.44-2.88 kJ·m−2·d−1 during cucumber and tomato scion and rootstock production in Plant Factory with Artificial Lighting could minimize plant damage while expecting to suppress grafting effects.
Speakers
DJ

dongcheol jang

assistatn professor, kangwon national university
Co-authors
HY

hwichan yang

kangwon national university
NA
SJ

soon jae hyeon

kangwon national university
NA
YK

youngho kim

kangwon national university
NA
YB

YunHyeong bae

kangwon national university
NA
Wednesday September 25, 2024 10:40am - 10:50am HST
South Pacific 3

10:45am HST

CHMG 1 - Cultivating Connections: Empowering Professionals through NC Farm to School Training Initiative
Wednesday September 25, 2024 10:45am - 11:00am HST
As the Farm to School movement continues to gain momentum, professionals in education, agriculture, and public health are seeking innovative ways to integrate this holistic approach into their practices. In North Carolina, our local, regional, and statewide food system has strengths across various sectors. Local agencies and community organizations now have decades of experience partnering together to maximize opportunities for local farmers and local food businesses. Partnering together, these food system practitioners have clearly begun to see that a systems approach is vital to supporting all sectors and stakeholders, vital to addressing the deep roots of inequity that negatively impacts our communities of color and particularly our children. The central need is in how to facilitate integrated efforts–both across farm to school components and also with multi-sector stakeholders–given that research shows that integration of farm to school programming brings the longest and most significant health and learning impacts on children. Our approach has been to develop the North Carolina Farm to School Training Initiative, a rich space of collaboratively created online content that informs and educates across the farm to school program areas. Through a combination of interactive modules, case studies, and expert-led discussions, our first course, School Gardening, provided participants with the knowledge and skills needed to navigate the complexities of Farm to School programming. The results from surveying pilot participants suggest numerous impacts, both on an individual and community level. Preliminary results found participants increased their knowledge and gained a deeper understanding of farm-to-school initiatives, including building community engagement through school garden teams, integrating gardening into curriculum, and the horticulture knowledge to successfully grow a garden. Participants improved their practical skills such as gardening techniques and the application of educational programming in the garden. Part of the course included interactive discussion boards and a qualitative review showed a number of themes emerged including that the course participants strongly benefitted by connecting with a group of like-minded educators and community organizers by fostering collaboration and support for farm-to-school efforts. Community engagement was another emergent theme with participants and they named that they would like to become more involved in their local food systems, finding connections with community partners like growers and Extension for access to resources and materials. These results suggest that fostering a community of practice that transcends geographic boundaries and provides localized and engaging content has significant potential to support great professional engagement in farm to school.
Speakers
LD

Liz Driscoll

Extension Associate, NC State University/ NC Cooperative Extension
Co-authors
KB

Kirsten Blackburn

NC State University
NA
RH

Remi Ham

NC State University
Wednesday September 25, 2024 10:45am - 11:00am HST
South Pacific 2

10:45am HST

HSMP 1 - Double-season production of hops (Humulus lupulus L.) with photoperiod manipulation in a subtropical climate
Wednesday September 25, 2024 10:45am - 11:00am HST
Hops (Humulus lupulus L.) have an ancient history of cultivation in temperate climates primarily as an ingredient of beer. However, growing hops in subtropical or tropical climates has been challenging because of non-optimal day length conditions. We examined the potential of subtropical hop production with photoperiod manipulation. ‘Cascade’ hops were grown in Florida, United States with extended day length (>16.5 hours) using light-emitting diode (LED) bulbs to inhibit flowering until sufficient vegetative growth was achieved. Phenology, plant growth, yield, and cone quality data were collected during the first two years after planting. With as little as 0.47 μmol/m2/s, photoperiod extension was effective in inhibiting flowering, while promoting vegetative growth. Uniform flowering was induced upon the discontinuation of photoperiod extension. As a result, plants achieved two growing cycles per year, with the spring and fall growing seasons running from February to July and July to December, respectively. Yield increased by 92% from Year 1 to Year 2 but decreased by 37% from the spring to fall growing season. The maximum yield was 465 kg/ha in Year 2 Spring. Cone quality was greatly affected by both year and season, with α acid, β acid, and total oil concentrations ranging from 2.7% to 6.2%, 1.9% to 3.2% and 0.14 to 0.53 mL/100 g, respectively. These results demonstrate that, with an aid of photoperiod manipulation for flowering control, ‘Cascade’ hops exhibit high phenological plasticity and acclimation capacity to subtropical climatic conditions. This study provides a foundation for developing the double-season hop production system in a subtropical climate.
Speakers
SA

Shinsuke Agehara

University of Florida
Co-authors
AA

Aleyda Acosta-Rangel

University of Florida
NA
JR

Jack Rechcigl

University of Florida
NA
Wednesday September 25, 2024 10:45am - 11:00am HST
South Pacific 1

10:45am HST

NUTS 1 - Evaluating Soil Management Impacts on the Pecan Orchard Mycobiome in the Semi-Arid Southwestern United States
Wednesday September 25, 2024 10:45am - 11:00am HST
Pecans hold significant agricultural importance in the water-limited Southwestern United States, underscoring the need for sustainable soil management practices in pecan cultivation. Recognizing the intricate relationship between soil treatments and the soil microbiome is essential to develop effective orchard soil management strategies. Soil fungi, particularly root-associated mycorrhizal fungi, are vital in facilitating water and nutrient uptake, protecting against pathogens, and enhancing overall orchard health and productivity. Soil management may impact the fungal community composition of Southwestern pecan orchard soils. Various soil management strategies are implemented in pecan orchards, including varying soil cover or applying mycorrhizal and bacterial inoculants. These techniques alter the soil environment, which may change the soil fungal biodiversity. This study investigates the impact of diverse soil management techniques on the soil mycobiome within a twelve-year-old ‘Pawnee’ pecan orchard in New Mexico. In a randomized complete block design, eight treatments of various soil cover – bare soil, cover crops, compost, or a combination of cover crops and compost – each either with or without mycorrhizal and bacterial inoculants, were applied to evaluate their effects on soil fungal diversity. Laboratory analyses, including DNA extraction, PCR amplification, and Illumina sequencing, were performed, alongside physiochemical testing for soil pH, electrical conductivity, and mineral nutrient content. The resulting sequence data were analyzed to provide insights into the complex interactions between soil management practices and microbial communities. Sampling conducted during the 2022 Spring and harvest seasons revealed significant differences in alpha and beta diversity between roots and bulk/rhizosphere soil (P < 0.10). Significant differences in alpha and beta diversity of fungi based on treatment were also observed, highlighting the potential influence of soil management practices, including soil cover and inoculant use, on fungal community composition. Our study offers valuable insights into the temporal changes in the community structure of pecan orchard fungi when treated with different soil amendments. Understanding how soil management practices influence the soil mycobiome can inform more sustainable pecan cultivation practices. By optimizing soil management strategies to support beneficial fungal communities, growers may enhance soil health, water and nutrient availability, and plant resilience to environmental stress.
Speakers
avatar for McKenzie Stock

McKenzie Stock

New Mexico State University
Co-authors
CV

Ciro Velasco-Cruz

New Mexico State University
NA
JR

Jennifer Randall

New Mexico State University
NA
NP

Nicole Pietrasiak

University of Nevada Las Vegas
NA
PG

Paul Gabriel

New Mexico State University
NA
RH

Richard Heeremaa

New Mexico State University
NA
Wednesday September 25, 2024 10:45am - 11:00am HST
Lehua Suite

10:45am HST

O/LT 1 - The Art and Technique of Producing Unique Lagerstroemia Plants
Wednesday September 25, 2024 10:45am - 11:00am HST
Crape myrtle (Lagerstroemia L.) stands as a ubiquitous presence in landscapes worldwide. Beyond its captivating smooth and exfoliating bark, a spectrum of flower colors, and impressive variable mature heights, the artistic modeling potential of crape myrtle has found favor in the high-end landscape market. Crafting a crape myrtle tree becomes a gratifying and imaginative endeavor. The preeminent modeled shapes include vases, screenings, letters, columnar forms, dragon-inspired (animalistic), symbolic representations, pavilions, tunnels, tree bonsai, ornamental root architecture, and even cartoon characters. Constructing a foundational armature from steel demands your artistic prowess and creativity, serving as the structural basis for the tree. Opting for fast-growing crape myrtle cultivars with pliable branches becomes imperative for success. Consistent pruning becomes a requisite to mold the growth pattern according to our artistic aspirations. Utilizing modeling wire facilitates the creation of the trunk and branch framework, with strategically tied crossed knots enhancing natural grafting unions. Developing distinctive Lagerstroemia plants requires a more extended timeframe and demands advanced modeling and pruning techniques compared to conventional growth processes. This production journey provides ample creative freedom and the ultimate performance should align with our artistic vision and the preferences of our clientele. Future studies should delve into plant growth dynamics and the development of trunk/branch anatomical structures to further enhance our understanding of this artistic horticultural practice.
Speakers
DZ

Donglin Zhang

University of Georgia
Co-authors
XL

Xi Li

Sichuan Agricultural University
NA
XH

Xiaoling Hou

Chongqing Yuexin Valley Ecological Agriculture Co. Ltd
NA
Wednesday September 25, 2024 10:45am - 11:00am HST
Coral 1

10:45am HST

PH 2 - Nutritional Quality and Shelf-Life of “Living Lettuce” Through 28 Days of Cold Storage
Wednesday September 25, 2024 10:45am - 11:00am HST
Lettuce (Lactuca sativa) is cultivated in controlled environment agriculture via vertical production or hydroponic well systems. To extend shelf-life, growers harvest “living lettuce” by keeping roots intact, but there is limited data supporting this harvesting technique. The objective of this study was to assess the use of clamshell and root treatments on the shelf-life and nutritional quality of lettuce. Treatments included storage either inside or outside of plastic clamshells and with roots removed or intact. Lettuce was stored for 28 days at 4 oC, 85% relative humidity. Percent weight loss (%weight loss), visual ratings (1 to 5 scale, 1 = poor and 5 = excellent), and colorimeter analysis (L*, a*, b*, c*, hue angle) were measured every 3 days. Total assays of chlorophyll, carotenoids, anthocyanins, flavonoids and phenolic activity were analyzed via UV VIS spectrophotometry every 7 days throughout storage. Few differences were seen in root treatment. As days in storage progressed, %weight loss progressed to 16.9%. Closed clamshells decreased %weight loss (5.5%) compared to open (18.1%). Visual ratings of yellowing/nutrient deficiency, relative greenness, wilt/head looseness, and salability decreased through storage. Lettuce stored in closed clamshells decreased %weight loss by 12% and delayed wilt/head looseness by 11 days. Similarly, lettuce stored in closed clamshells with roots were salable until 18 days in storage, while open clamshells were no longer salable after 7 days. Short root length (~4 cm) may be the dominant factor for the lack of significance as previous research suggested harvesting “living lettuce” with 9 cm roots increased storage and quality. Color data differed, and lettuce stored in closed clamshells with roots had more intense red color a* (-3.7 vs. -2.7), blue color b* (12.6 vs 10.1), and were purer in color c* (13.3 vs. 10.5) compared to open clamshells with roots. No differences were seen in total anthocyanin (mg/L), total chlorophyll (g/g) or total carotenoid (g/g) content in clamshell or root treatment (p > 0.05). Through 14 days of storage, total anthocyanins degraded by 7.55 mg/L (Day 0 = 10.78 vs. Day 14 = 3.22), while no loss of total chlorophyll (9204.84 g/g) or total carotenoids (1947.25 g/g) were seen. Anthocyanins are water soluble pigments and with increased weight/water loss, anthocyanins solubilize leading to degradation. Chlorophylls and carotenoids were not lost, potentially due to low enzyme activity of chlorophyllase. This experiment indicates consumers should eat lettuce within 7-10 days of harvest for highest nutritional and postharvest quality.
Speakers
MT

Marlee Trandel-Hayse

Assistant Professor of Postharvest Physiology, Auburn University
High nutritional quality, good texture and long shelf-life are critical for consumer acceptance of fruit and vegetable crops. As the assistant professor of Postharvest Physiology at Auburn University, my research addresses the impact of preharvest systems on postharvest quality. I... Read More →
Co-authors
CR

Camila Rodrigues

Auburn Univeristy
NA
CB

Caroline Blanchard

Auburn University
DW

Daniel Wells

Auburn University
NA
MH

MD Hasibur Rahman

Auburn University
NA
TR

Tanzeel Rehman

Auburn University
NA
Wednesday September 25, 2024 10:45am - 11:00am HST
Nautilus

10:45am HST

VSF 2 - Genome Database for Vaccinium: Genomics Data and Tools to Facilitate Research
Wednesday September 25, 2024 10:45am - 11:00am HST
The Genome Database for Vaccinium (GDV, www.vaccinium.org) is a community database resource for Vaccinium researchers and breeders. The database contains genomics data for blueberry, cranberry, bilberry, and lingonberry that are integrated with other types of data such as genetic maps, markers, and QTL/GWAS. The genomics data is accessible via a plethora of search interfaces and tools. GDV has over 40 genomes and allows users to view the genomes in JBrowse, query sequences against the genomes in BLAST and view genome synteny via the Synteny Viewer tool. Highly utilized genomes in JBrowse contain tracks of aligned markers, GWAS, and QTL. Standard GDV analyses on the genomes include synteny analysis and functional annotation of predicted genes with InterPro protein domains and GO terms as well as homology to known proteins. The genes and mRNAs of each genome, along with the functional annotations and orthologs/paralogs, are searchable on GDV. Representative genomes are available in the PathwayCyc tool which displays metabolic pathways and allows for transcriptomics or metabolomics data overlays. GDV also has the genomes and structural pangenomes from the Vaccinium Pangenome Project, a product of VacCAP. Expression datasets that are mapped to genomes are also available to explore using the Expression Heatmap tool. Also available in GDV is the Breeding Information Management System (BIMS) that allows breeders to manage and analyze the phenotypic and genotypic data and provides public access to GRIN phenotype data. This presentation will showcase how to access these genomics data through various tools.
Speakers
avatar for Jodi Humann

Jodi Humann

Research Associate, Washington State University
Co-authors
CH

Chun Huai Cheng

Washington State University
NA
DM

Dorrie Main

Washington State University
KB

Katheryn Buble

Washington State University
NA
MI

Massimo Iorizzo

North Carolina State University
NA
PZ

Ping Zheng

Washington State University
NA
SJ

Sook Jung

Washington State University
NA
TL

Taein Lee

Washington State University
NA
Wednesday September 25, 2024 10:45am - 11:00am HST
Kahili

10:45am HST

WUM 1 - Physiological Thresholds Provide Targets for Climate-Resilient Irrigation Management in Hazelnut
Wednesday September 25, 2024 10:45am - 11:00am HST
The rapidly changing climate is creating challenges for the selection and management of woody perennial crops. For North American (NA) cultivars of hazelnut (Corylus avellana), there is insufficient information on water stress management to maintain physiological performance and optimize productivity under limited soil water availability. Current plantings of NA hazelnuts are predominantly comprised of cultivars resistant to biotic stress (e.g., Eastern Filbert Blight) such as ‘Jefferson’ and ‘Yamhill’ cultivars, but their responses to abiotic stressors exacerbated by climate change is unknown. Our research objectives were to: 1. identify cultivar-specific physiological thresholds in response to water stress such as negligible leaf gas exchange (i.e., stomatal closure) and onset of leaf wilting (i.e., cell turgor loss) for phenotyping in greenhouse conditions; and 2. relate vapor pressure deficit to plant water status in order to generate a water-potential baseline capable of differentiating between atmospheric and soil moisture impacts on water stress in field conditions. Using the water potential (Ψ) curve (WPC) method, stomatal closure was initiated at less negative Ψ in ‘Jefferson’ (-0.85 MPa) compared to ‘Yamhill’ (-1.1 MPa). Similarly, turgor loss was found to occur at less negative Ψ in ‘Jefferson’ (-1.26 MPa) compared to ‘Yamhill’ (-1.48 MPa). These cultivar-specific differences were confirmed with direct measurements of stomatal conductance using a porometer and an evaluation of turgor loss point using the pressure-volume curve method. In the field, we established a water potential baseline to distinguish between the effects of soil moisture and vapor pressure deficit on Ψ. Our field results found a deviation from baseline of -1.0 MPa resulted in stomatal closure in Yamhill, which was consistent with our prediction from the WPC. ‘Yamhill’ trees that had Ψ on average -0.68 MPa below baseline over the growing season were also observed to have 34% smaller nuts, 46% higher shell-to-kernel ratio, and an estimated 50% of total in-shell yield. Upcoming research will seek to replicate results experimentally with both cultivars. In summary, our results indicate that the WPC is a valid tool for physiological phenotyping and preliminary results suggest that thresholds from the WPC provide viable cultivar-specific targets for improving irrigation management in hazelnuts. These results highlight methods to help determine sustainable irrigation management targets that can help conserve water resources strained by climate change while also maintaining plant productivity.
Speakers
SB

Steven Bristow (He/Him)

University of British Columbia
NA
Co-authors
TK

Thorsten Knipfer

University of British Columbia
NA
Wednesday September 25, 2024 10:45am - 11:00am HST
South Pacific 4

10:50am HST

NUR/RHIZ - Effects of Nitrogen on the Nodulation of Ceanothus velutinus
Wednesday September 25, 2024 10:50am - 11:00am HST
Ceanothus velutinus, commonly known as snowbrush ceanothus, is a nitrogen-fixing species native to North America. It plays a vital role in ecosystem by improving soil fertility through nodulation, a symbiotic process with bacteria that fixes atmospheric nitrogen. The purpose of this study was to identify the effects of various nitrogen concentrations on the nodulation and plant morphological and physiological responses. Ceanothus velutinus seedlings were transplanted in calcined clay and inoculated with 30 mL of soil containing Frankia. Seedlings were treated with 0.0 to 8.4 g·L-1 of controlled released fertilizer (CRF, 15N-3.9P-10K) or a nitrogen-free nutrient solution supplemented with or without 2mM ammonium nitrate (NH4NO3). Plant growth and photosynthesis increased linearly or quadratically along with the increasing CRF application rates with a notable increase observed at 4.2 g of CRF. Nodules were observed only in plants receiving 0.0, 0.3, 0.5, 1.1, or 2.1 g of CRF. However, the number of nodules formed in the treatments was too small to analyze statistically. The study indicates that while CRF significantly boosts C. velutinus growth, nodulation and nitrogen fixing capacity of the plant remains unknown. Further investigation is needed to determine the effect of nitrogen on the nodulation of C. velutinus using peat-based soilless substrate.
Speakers
PN

PRAKRITI NEPAL

Utah State Univeristy
Co-authors
YS

Youping Sun

Utah State Univeristy
Wednesday September 25, 2024 10:50am - 11:00am HST
South Pacific 3

11:00am HST

CHMG 1 - The gARTening Program: Fostering Community Engagement through Innovative Education
Wednesday September 25, 2024 11:00am - 11:15am HST
Orange County, Florida, an urban county with a boasting a population of 1.4 million residents, faces a distinctive demographic trend with a homeowner rate of 56%, notably below the national average of 64%. This indicates a higher proportion of renters and apartment dwellers within our community. In order to expand the reach of our horticultural efforts beyond the limitations of traditional homeownership and include all residents, our Master Gardener Volunteer Program developed the Garden Art (gARTening) program, aimed to appeal to a wider audience. Beyond mere attraction of new attendees, our focus was to increase: office foot traffic, awareness of UF/IFAS Extension Services, and create a unique hands-on program that maintained affordability while generating revenue. We developed "The gARTening Program," featuring unique garden-related art projects, to achieve our objectives. Classes ranged from 1-2 hours and $5-35, with topics such as terrariums, kokedamas, pressed flower art, and more. Collaborating with Master Gardener Volunteers (MGVs), we created educational materials such as PowerPoints, handouts, and economical shopping lists. Our diverse approach attracted a broad audience, including young families, mother-daughter pairs, couples, and individuals, fostering inclusivity and engagement. The outcomes of the gARTening Program exceeded expectations: • Revenue generated in one year: $3200 • 47% of participants were new to UF/IFAS Extension and had never utilized our services before. • 70% joined due to the unique hands-on experience. • 95% indicated they gained knowledge of UF/IFAS Extension services as a result of attending the workshop. • Additionally, participants showed continued engagement, with many signing up for additional classes, volunteering with 4-H or becoming an MGV, and promoting our services to others. The gARTening Program not only met, but surpassed its objectives, illustrating its efficacy in community engagement and revenue generation. By offering unique experiences, we heightened awareness of UF/IFAS Extension Services, attracting new participants and fostering long-term engagement. The program's success underscores the potential for innovative education methods to address funding challenges while advancing community outreach and awareness. This model holds broader implications for enhancing community well-being and supporting sustainable organizational growth.
Speakers
avatar for Clarissa Chairez

Clarissa Chairez

Extension Faculty, UF/IFAS Extension Orange County
Wednesday September 25, 2024 11:00am - 11:15am HST
South Pacific 2

11:00am HST

HSMP 1 - Brewing Conditions and UV treatment affect Shelf Life of Cold-brew US-grown Tea
Wednesday September 25, 2024 11:00am - 11:15am HST
Tea, Camellia sinensis, is grown around the world under diverse geographic and climatic conditions and can be cultivated as a specialty crop in the U.S. U.S. specialty tea growers cater to the high-quality specialty tea niche market, and innovations in mechanization and novel products increase profitability. The objective of this study was to develop a cold brew methodology that would maximize the flavor characteristics and shelf life of cold brew black teas to facilitate the development of new products. 'Black Magnolia' from the Great Mississippi Tea Co. (Brookhaven, MS) was used to determine combinations of cold brew conditions affecting taste and health-promoting compositions (polyphenol and caffeine), including selected combinations of tea-to-water ratio (1, 2, and 3g of tea per cup of water), temperature (40, 45, 50, 55, and 60°F), brewing time (6, 8, 10, 12, and 14 hours), and particle size (whole-leaf, coarse, and fine). Selected conditions were then used in a UV light experiment to compare shelf life (microbial growth at 2, 4, and 6 days after brewing) using hot water-brewed tea as a control. Results indicate that, for this specific black tea, 2g per cup at higher temperatures of 55 to 60°F resulted in comparable polyphenol contents to hot water brew, but lower caffeine concentrations. However, compared to lower temperatures, teas made at this range of temperatures resulted in a higher number of yeast and mold by day 4. Brewing at 45°F for 14 hours resulted in comparable polyphenol contents as hot water brew and was described with more positive words compared to a shorter brewing time during sensory surveys conducted on campus with over 100 students. Initial testing of UV light treatments for 0, 0.5, 1.0, and 2.0 hours of duration before cold brew indicated that UV light is insignificant in reducing microbial growth. Further research is needed to improve the UV treatment or use other methods to extend the shelf life of cold brew tea. The best brewing conditions obtained from this study could be of guidance for future large-scale consumer sensory evaluations that will compare the flavor of four U.S.-grown black teas to imported specialty teas and their health-benefiting compounds. This next study will provide essential and important information for U.S. tea growers and consumers on how U.S.-grown tea performs in comparison with internationally known black teas and potential enhancements to maximize the health benefits and popularity of U.S.-grown specialty tea.
Speakers
YC

Yan Chen

LSU AgCenter
Co-authors
ZX

Zhimin Xu

Louisiana State University School of Nutrition and Food Sciences
NA
Wednesday September 25, 2024 11:00am - 11:15am HST
South Pacific 1

11:00am HST

O/LT 1 - Effects of Different Pruning Regimes on Growth Reallocation and Carbon Storage in Buxus microphylla var. japonica ‘Winter Gem’
Wednesday September 25, 2024 11:00am - 11:15am HST
Hedge shaping and size maintenance is often accomplished with electric or gas-powered shears due to a lower cost compared to hand pruning. Shearing plants arbitrarily removes the apical growing points from external portions of the shrub to achieve a desired shape and size of the plant and often results in poor quality cuts, leaving ragged ends of woody tissues or leaves. Contrarily, hand pruning makes strategic, ‘clean’ cuts often back to lateral branches to achieve these goals. Use of plant growth regulators like paclobutrazol (PBZ) can reduce the frequency of pruning and could be a useful component of a hedge management program. The purpose of this study was to investigate the effects of shearing, hand pruning, and/or PBZ application on regrowth of foliage and non-structural carbohydrates (NSCs) of ‘Winter Gem’ boxwood over time. Fifteen shrubs each per pruning type x frequency combination were pruned with bypass hand pruners (hand pruned) or gas-powered shears (sheared) in 2021, 2022, and 2023 once or twice per growing season. In addition, another 15 shrubs each were sheared once followed by an immediate application of a foliar PBZ (i.e. Trimtect®) in accordance with the label using an electric backpack or left as non-pruned controls. In 2021 and 2022, shrubs were pruned by removing 15 percent of the overall height and 20% of the overall widths in two perpendicular directions of each shrub. In 2023, pruned shrubs were cut back to the previous season’s overall height and width. Regrowth was measured by weighing the fresh biomass removed at each pruning and NSCs were measured from ten woody twigs from the exterior of each shrub using the phenol-sulfuric acid quantification method. Two and three years after pruning, shearing shrubs twice had significantly more biomass produced year over year compared to hand pruning, while PBZ treated shrubs had the least amount of regrowth. NSCs trended to be highest in shrubs that were hand pruned once or in PBZ treated shrubs, while the least in shrubs that were sheared twice. PBZ-treated shrubs had tighter clusters of internodes resulting in approximately 30% reduction in stem elongation compared to controls. The differences in growth dynamics and carbon storage across these different pruning strategies can have different long- and short-term implications in managing boxwood hedges, which will be presented here.
Speakers
AL

Andrew Loyd

Plant Pathologist, Bartlett Tree Research Laboratories
Co-authors
CL

Caitlin Littlejohn

Bartlett Tree Research Laboratories
NA
CR

Chad Rigsby

Bartlett Tree Research Laboratories/Morton Arboretum
NA
Wednesday September 25, 2024 11:00am - 11:15am HST
Coral 1

11:00am HST

PH 2 - Comparing the Postharvest Phytonutrient Content of Red and Green Butterhead Lettuce Cultivars
Wednesday September 25, 2024 11:00am - 11:15am HST
Lettuce is a popular leafy vegetable that can play an important role in human nutrition and diets with regular consumption. Lettuce cultivars can vary considerably in their postharvest nutritional composition. It is critically important to determine which lettuce cultivars have the highest nutritional quality to allow growers to select those that are best for their market. The objectives of this experiment were twofold, to determine differences in the phytonutrient content of two butterhead lettuce cultivars, ‘Nancy’ (green butterhead) and ‘Skyphos’ (red butterhead), and if leaf location within the head (lower or mid leaves) differs in nutritional composition. The experiment was conducted in the spring of 2023 in a high tunnel, and setup as a completely randomized design with 4 replications. At horticultural maturity (40 days after germination), the lower and mid leaves were harvested. Lettuce samples were frozen and shipped to Auburn University where samples were assayed for total chlorophyll, carotenoids, anthocyanins, flavonoids, phenolics and antioxidant activity via UV VIS spectrophotometry. Carotenoids of violaxanthin, neoxanthin, lutein and -carotene were quantitated by UPLC/MS. The interaction of leaf location*cultivar (P < 0.05) indicated that the lower leaves of ‘Skyphos’ lettuce had the highest total anthocyanin content at 2.94 mg/L compared to ‘Nancy’ at 0.05 mg/L, respectively. No other differences (P > 0.05) were detected between the cultivars. Sample location differed in total carotenoids (mg/g) and total chlorophyll (mg/g) with the lower leaves having the highest nutritional composition at 1380.18 and 5973.58 mg/g, respectively compared to the mid leaves (509.02 and 1684.42 mg/g, respectively). Chlorophyll a (4399.67 mg/g) and b (1573.90 mg/g) was also highest in the lower leaves of both cultivars compared to the mid leaves. Our results indicate that both butterhead lettuce cultivar and leaf location strongly impacts human health. ‘Skyphos’, a red butterhead lettuce, had the high highest total anthocyanin content in the lower leaves. Anthocyanins are critical phytochemicals known for many health promoting properties such as free radical scavenging, and anti-cancer and anti-diabetic properties. This experiment also indicated the lower leaves of both cultivars had the highest amount of total chlorophyll and carotenoids. The lower leaves of red butterhead lettuces, such as ‘Skyphos’, should be eaten to obtain the highest nutrition for their diet.
Speakers
MT

Marlee Trandel-Hayse

Assistant Professor of Postharvest Physiology, Auburn University
High nutritional quality, good texture and long shelf-life are critical for consumer acceptance of fruit and vegetable crops. As the assistant professor of Postharvest Physiology at Auburn University, my research addresses the impact of preharvest systems on postharvest quality. I... Read More →
Co-authors
AW

Alan Walters

Southern Illinois University
Wednesday September 25, 2024 11:00am - 11:15am HST
Nautilus

11:00am HST

VSF 2 - Use of glycine betaine and kelp extract mitigates heat stress in red raspberry (Rubus idaeus)
Wednesday September 25, 2024 11:00am - 11:15am HST
Heat stress poses a significant threat to global food production and security by disrupting plant physiological and biochemical processes. Global simulation models predict a 4 to 5°C increase in atmospheric temperatures by the year 2100, as well as increases in the frequency of extreme heat events. This has necessitated preemptive measures to enhance the resiliency of horticultural crop production. Biostimulants, a class of agricultural products, show great promise in mitigating heat stress effects by enhancing physiological and biochemical stress tolerance. However, limited research exists on the efficacy of commercial biostimulant products in improving heat stress tolerance in horticultural crops. This study evaluated the impacts of three exogenously applied commercial biostimulants representing a range of active ingredients and included FRUIT ARMORTM, Optysil®, and KelpXpressTM [active ingredients glycine betaine, silicone, and kelp (Ascophyllum nodosum) extract, respectively], plus a water control, on physiological, biochemical, and growth parameters in different raspberry genotypes exposed to continuous heat stress (Tmax ≥ 35°C/day) in a glasshouse. Over a 28-day period, the biostimulants and the water control were applied weekly to three raspberry genotypes (‘Meeker’, WSU 2188, and ORUS 4715-2). The results indicated that ‘Meeker’ consistently maintained high chlorophyll fluorescence (Fv/Fm) and photosynthesis under control and biostimulant treatments. In contrast, WSU 2188 and ORUS 4715-2 exhibited increased Fv/Fm and photosynthesis when treated with FRUIT ARMORTM. Additionally, KelpXpressTM application improved Fv/Fm in WSU 2188. ‘Meeker’ and WSU 2188 treated with FRUIT ARMORTM and KelpXpressTM accumulated more anthocyanins and had greater shoot and total biomass compared to ORUS 4715-2. These findings underscore genotype-specific and biostimulant-dependent responses to heat stress mitigation. The superior physiological performance by ‘Meeker’ under both control conditions and biostimulant treatments indicates heat tolerance in the genotype. Furthermore, higher anthocyanins accumulation, improved Fv/Fm, enhanced gas exchange, and greater total biomass of WSU 2188, as well as improved Fv/Fm of ORUS 4715-2, suggests that application of the biostimulants contributed to enhanced repair and maintenance of photosystem II (PSII) structural integrity, improved photosynthetic performance, and increased antioxidative capabilities, which may have contributed to higher total biomass of raspberry treated with FRUIT ARMORTM relative to the untreated control. In summary, the positive impacts on physiological, biochemical, and growth parameters support the potential role of biostimulants in enhancing thermotolerance in raspberries and other horticultural crops exposed to heat stress.
Speakers
MG

Makonya Givemore Munashe

Washington State University
Co-authors
Wednesday September 25, 2024 11:00am - 11:15am HST
Kahili

11:00am HST

WUM 1 - Determination of Crop Coefficient and its Inter-annual Variability in Pecan Orchard in Georgia
Wednesday September 25, 2024 11:00am - 11:15am HST
Pecans have high economic importance in the US. Nonetheless, as one of the top pecan producers, there is little research on water use of pecan trees in the Southeast of the US. The water status of the tree impacts the yield, mostly during the kernel filling period (August and September). The knowledge gap of pecan water requirements stems largely from the Southwest. There, pecan tree needs in the hot and arid climate of the Southwest contrast sharply with those of the long, hot and humid Southeastern climate. Furthermore, the Southwest management practices use flood irrigation in contrast with most Georgia orchards which use micro-irrigation. This paper reports on the development of a crop coefficient specifically addressing the pecan tree needs in the Southeastern US. This study uses an eddy-covariance system and micro-lysimeter to determine the actual evapotranspiration of pecans. The potential evapotranspiration is determined using nearest local weather station data. This paper discusses the behavior of the crop coefficient throughout the different physiological stages of the tree from budbreak to harvest. Results of the crop coefficient obtained throughout the season differs from the Southwest, where the actual evapotranspiration during the growing season is significantly higher than the one observed in the Southeast. The daily and monthly crop coefficient throughout the growing period from 2019 through 2023 respectively are discussed. The year-to-year variability is also discussed. These results should support pecan growers and researchers alike to more tailored irrigation schedule in Southeast pecan orchards.
Speakers
KP

Kriti Poudel

University of Georgia
Co-authors
GZ

Gengsheng Zhang

The University of Georgia
NA
LW

Lenny Wells

University of Georgia
NA
ML

Monique Leclerc

University of Georgia
NA
Wednesday September 25, 2024 11:00am - 11:15am HST
South Pacific 4

11:00am HST

Lessons from a Journey: Navigating Your Path Through College and Beyond
Wednesday September 25, 2024 11:00am - 11:30am HST
In this engaging talk, recently retired professor, Elizabeth Mitcham reflects on her career journey to offer valuable advice to students. Drawing from personal experiences and professional milestones, Professor Mitcham will share insights on making choices throughout your career, seizing opportunities, and maintaining work-life balance. The talk covers essential topics such as the importance of networking, knowing what you value most, and the benefits of pursuing diverse interests. By weaving these lessons into a narrative of her own career, Professor Mitcham aims to inspire students to navigate their academic and professional paths with confidence, resilience, and purpose.
Wednesday September 25, 2024 11:00am - 11:30am HST
Coral 5 - ASHS Hort Theater

11:14am HST

Undergraduate Student (UG)
Wednesday September 25, 2024 11:14am - 12:45pm HST
A Comparative Study Analyzing Light Lengths for the Growth of Rex Butterhead Lettuce Utilizing GREENBOX Technology - Mya Griffith
Evaluating the Feasibility of Lettuce Crop Cultivation with Reclaimed Water Utilizing GREENBOX Technology - Mya Griffith
The Assessment of Different Growth Mediums for Plug Cultivation in a Controlled Environment - George Buss
Comparative Analysis of Lettuce Growth Using Compost Versus Conventional Soil - Sofia Huber
Feasibility of Plug Production Utilizing Digestate From Home Water-Energy-Food Systems (H-WEF) - Rory Dunn
From Flower to Fruit: Growing Degree Days and Peach Ripening - Matthew Almy
Enhancing rose propagation using moisture sensor-controlled irrigation and LED supplemental lighting in greenhouses - Braylen Thomson
Wednesday September 25, 2024 11:14am - 12:45pm HST
Lehua Suite

11:15am HST

CHMG 1 - Welcome Home to Gardening in Tennessee: Reaching New Audiences with New Collaborative Outreach Models
Wednesday September 25, 2024 11:15am - 11:30am HST
New residents in the state along with new gardeners are an increasingly large Extension audience. As our Extension horticulture team discussed the challenges in individually addressing these questions and meeting the needs of these new stakeholders, we realized new models and resources were needed. While Extension has a great array of publications and resources on lawns, landscapes and gardens, there wasn’t a publication that provided a good entry point for those new to the region or gardening. So, we set out to develop a ‘gateway publication’ to provide a general overview and connect folks to further resources without being overwhelming. A magazine format with easy to digest 2-page spreads on the most common areas of gardening was developed titled “Welcome Home: Gardening in Tennessee.” To extend the reach and impact of this new resource and engage Extension agents and stakeholders across the state, our horticulture team developed a new outreach program that paired live, distance teaching with local hands-on labs and activities. In October of 2023, we had a 5-session workshop series where new residents could come to their local office for an hour of teaching from our best horticulture content folks and then take part in a hands-on teaching demonstration with their local agriculture Extension agent. Nearly 300 participants in 32 counties took part in the series. Evaluation data revealed that over 70% of attendees had attended no or very few prior Extension programs. With a fee of only $30-50 locally, attendees reported a value of over $950 received from information delivered in the class. This presentation will focus on the novel delivery model and evaluation data along with perspectives on opportunities and challenges in reaching these new Extension audiences.
Speakers
NB

Natalie Bumgarner

Assistant Professor, University of Tennessee Knoxville
Co-authors
AL

Andrea Ludwig

University of Tennessee
NA
AD

Anna Duncan

University of Tennessee
NA
CS

Celeste Scott

University of Tennessee
NA
GU

Gregg Upchurch

University of Tennessee
NA
HT

Haley Treadway

University of Tennessee
NA
JS

Justin Stefanski

University of Tennessee
NA
LR

Lee Rumble

University of Tennessee
NA
MR

Melody Rose

University of Tennessee
NA
MM

Mitchell Mote

University of Tennessee
NA
SW

Seth Whitehouse

University of Tennessee
NA
TR

Taylor Reeder

University of Tennessee
NA
VS

Virginia Sykes

University of Tennessee
Wednesday September 25, 2024 11:15am - 11:30am HST
South Pacific 2

11:15am HST

HSMP 1 - Withering Duration and Drying Temperature Significantly Affect Postharvest Quality of Kratom (Mitragyna speciosa)
Wednesday September 25, 2024 11:15am - 11:30am HST
Kratom (Mitragyna speciosa) is an ethnobotanical plant with high medicinal value that has been historically utilized in Southeast Asia as a traditional remedy for fatigue mitigation and productivity enhancement. Recently, it has attracted widespread attention, particularly in North America and Europe, for its potential for pain management and alleviating opioid withdrawal symptoms and has become an important source for future drug development. Postharvest processing represents a key step in the kratom farm-to-pharm chain where products undergo chemical modifications before reaching consumers. In this study, we investigated how different withering durations, drying temperatures, and lighting conditions could affect kratom postharvest product qualities. Leaves were harvested from cultivar H and mixed well before randomly assigned to one of four withering durations (0, 12, 24, and 72 h) at 25 ℃ in the dark and subsequently one of five drying temperatures (-40, 25, 40, 60, and 80 ℃). Additionally, leaves were dried at 25 ℃ under either light or dark. Overall, withering at 25 ℃ significantly increased mitragynine concentration. Compared to 0 h withering, a 12 h withering followed by drying at -40, 25, 40, or 60 ℃ increased mitragynine concentrations by 117%, 17-123%, 16-61%, and 43-103%, respectively. The 12 h withering increased the concentration of speciogynine and paynantheine by 27-28% and 35-67%, respectively, when leaves were dried below 40 ℃. In contrast, speciociliatine levels initially decreased during 12 or 24 h withering but subsequently elevated after the withering duration increased to 72 h. Drying temperatures and light exposure generally had little effect on the biosynthesis of most of the alkaloids. However, an alternation in kratom powder color was noticed for those with a short withering duration and subsequently a high drying temperature. Other minor alkaloids including corynoxine A, corynoxine B, speciofoline, isospeciofoline, mitraphylline, and ajmalicine were below the lower limit of quantifications. Taken together, our study shows that withering and subsequent drying temperatures have significant effects on the color and content of bioactive compounds of kratom, and further research on optimizing kratom postharvest processing is needed.
Speakers
MZ

Mengzi Zhang

Biological Scientist, University of Florida
Co-authors
BP

Brian Pearson

Oregon State University
NA
JC

Jianjun Chen

University of Florida
NA
Wednesday September 25, 2024 11:15am - 11:30am HST
South Pacific 1

11:15am HST

O/LT 1 - Establishment, Growth, and Physiology of Container-Grown Trees Following Root Remediation at Planting
Wednesday September 25, 2024 11:15am - 11:30am HST
Root defects, especially circling roots, are a major concern when planting container-grown trees. In this study, we compared survival, crown dieback, and plant water potential of four common landscape tree species (Carpinus caroliniana, Liriodendron tulipifera, Ostrya virginiana, and Platanus × acerifolia) in response to root modifications (control, bare-root washing, shaving, and vertical slicing) prior to planting. P. × acerifolia trees were robust with respect to root correction treatments and had 100% survival except for some mortality following vertical root-ball slicing. In contrast, C. caroliniana, L. tulipifera, and O. virginiana trees had significant mortality and crown dieback in response to bare-root washing. The responses of these species to bare-root washing reflected extreme plant moisture stress immediately after planting. These three species are also considered ‘difficult to transplant’ as bare-root nursery stock. Our results suggest that trees that are generally known to be difficult to transplant as bare-root stock are poor candidates for extreme root disturbance such as bare-rooting when grown as container trees. In contrast, shaving and vertical slicing had little or no adverse effects on tree survival, crown dieback, or plant water potential.
Speakers
avatar for Bert Cregg

Bert Cregg

Michigan State University
Dr. Bert Cregg is a professor of Horticulture and Forestry at Michigan State University. He conducts research and extension programming on the physiology and management of trees in landscapes and nursery and Christmas tree production.
Co-authors
RJ

Riley Johnson

Michigan State University
NA
Wednesday September 25, 2024 11:15am - 11:30am HST
Coral 1

11:15am HST

PH 2 - Production System Alters Phytonutrient Content of ‘Skyphos’ Butterhead Lettuce
Wednesday September 25, 2024 11:15am - 11:30am HST
Lettuce is one of the most widely consumed vegetables in the world and can provide various health benefits to consumers. The type of production system, such as high tunnel, green roof or open field environments, can influence the nutritional composition of lettuce. To determine the extent of phytonutrient content change, ‘Skyphos’ lettuce was grown in these various production environments to compare phytonutrient content at harvest. The production experiment was conducted at Southern Illinois University-Carbondale and set up with three treatment locations and four replications. Two production systems used organic fertility practices (high tunnel and green roof), while the field production system used conventional fertilizers. At horticultural maturity (40 days after germination), the lower and mid leaves were harvested to determine phytonutrient content. Lettuce samples were frozen and shipped to Auburn University where samples were assayed for total chlorophyll, carotenoids, anthocyanins, flavonoids, phenolics and antioxidant activity via UV VIS spectrophotometry. Carotenoids of violaxanthin, neoxanthin, lutein and b-carotene were quantitated by UPLC/MS. The interaction of production system*leaf sampling location differed (P < 0.05) in total anthocyanins, total carotenoids and total chlorophyll. Total anthocyanins were highest in the lower leaves of ‘Skyphos’ lettuce cultivated on the green roof at 3.57 mg/L. In comparison, total carotenoids, total chlorophyll and chlorophyll a were highest in the lower leaves of lettuce cultivated in the high tunnel at 1316.48, 6093.45 and 4401. 97 mg/g, respectively. Cultivating lettuce in the high tunnel provided the highest Chlorophyll b content (1638.37 mg/g) compared to the field (888.80 mg/g) or green roof (893.43 mg/g). The phytonutrient content of lettuce can be influenced by location, temperature, soil type, humidity and UV radiation. The green roof increased total anthocyanin content which may be due to the higher temperatures and irradiation levels on compared to the field or high tunnel. Total Chlorophyll and carotenoid content was increased in the high tunnel likely due to increases of UV-A and UV-B exposure from the poly film leading to an increase in photosystem response. This experiment suggests the specific phytonutrient content (e.g., anthocyanin or carotenoids) in a red lettuce like ‘Skyphos’ differs based on production system and can be used in market development to increase consumer consumption by highlighting the specific phytonutrients highest in each production system.
Speakers
MT

Marlee Trandel-Hayse

Assistant Professor of Postharvest Physiology, Auburn University
High nutritional quality, good texture and long shelf-life are critical for consumer acceptance of fruit and vegetable crops. As the assistant professor of Postharvest Physiology at Auburn University, my research addresses the impact of preharvest systems on postharvest quality. I... Read More →
Co-authors
AW

Alan Walters

Southern Illinois University
Wednesday September 25, 2024 11:15am - 11:30am HST
Nautilus

11:15am HST

UG - Evaluating the Feasibility of Lettuce Crop Cultivation with Reclaimed Water Utilizing GREENBOX Technology
Wednesday September 25, 2024 11:15am - 11:30am HST
The development of GREENBOX technology addresses the challenges posed by rapid population growth, which intensifies the demand for agricultural resources essential for cultivating and distributing fresh produce, including arable land, water, and nutrients, to both rural and urban areas. Utilizing principles of Controlled Environment Agriculture (CEA), GREENBOX technology optimizes growth conditions for leafy green crops by leveraging existing urban infrastructure and readily available commercial equipment. GREENBOX technology allows for precise control over environmental variables such as temperature, humidity, light intensity/spectrum, and nutrient delivery, thereby enhancing the growth performance of leafy greens. We were interested in assessing the feasibility of utilizing reclaimed water for crop production as preliminary experiments employing GREENBOX technology that employed a standard nutrient solution comprising a blend of 5-12-26 and 15-0-0 Calcium Nitrate for crop production. This study's primary objective was to conduct a comparative analysis of Lactuca sativa Rex Butterhead Lettuce production using the standard nutrient solution as the control (Treatment 1), and Reclaimed water or treated wastewater supplemented with additional nutrients (Treatment 2). The assessment focused on measuring crop biomass and productivity and environmental conditions associated with each nutrient solution to identify any significant differences. Biomass parameters, including wet weight, dry weight, leaf area, leaf count, and chlorophyll concentration, were measured alongside derived data such as Leaf Area Index (LAI), Specific Leaf Area (SLA), and biomass productivity. Statistical analysis of the biomass data was conducted to discern differences in biomass parameters between crop growth using both hydroponic solutions. Both treatments yielded Rex Butterhead lettuce well above the anticipated harvest weight of 180g, indicating their suitability for crop production in urban warehouse settings. The findings of this experiment contribute valuable insights into the feasibility of utilizing various types of wastewater for hydroponic crop growth. Future experiments employing GREENBOX technology may utilize these findings to enhance the efficiency, productivity, and sustainability of GREENBOX units. This study has impactful implications for sustainability, as it offers a potential solution to mitigate water scarcity and promote efficient resource utilization in agricultural practices. Keywords: CEA, Hydroponics, lettuce, Reclaimed Wastewater, urban agriculture
Speakers
MG

Mya Griffith

Florida Gulf Coast University
Co-authors
AS

Ankit Singh

University of Maine
NA
BR

Barry Rosen

Florida Gulf Coast University
NA
GP

Galen Papkov

Florida Gulf Coast University
NA
GB

George Buss

Florida Gulf Coast University
JG

John Griffis

Florida Gulf Coast University
NA
KJ

Kathryn Jackson

Independent Researcher
NA
PC

Paige Carroll

Florida Gulf Coast University
NA
SB

Sarah Bauer

Mercer University
NA
XY

Xiusheng Yang

University of Connecticut
NA
Wednesday September 25, 2024 11:15am - 11:30am HST
Lehua Suite

11:15am HST

WUM 1 - Assessing Water Status in Citrus Plants Using Thermal Imaging in Greenhouses
Wednesday September 25, 2024 11:15am - 11:30am HST
Thermal cameras can easily determine plant canopy temperature, and the resulting data can be used for irrigation scheduling in addition to other water management tools. This study aimed to develop a method to use thermal imaging for canopy temperature measurements in one-year-old citrus plants to assess citrus water status. We evaluated the influence of five water levels (25%, 50%, 75%, 100%, and 125%) based on the crop evapotranspiration replacement of two citrus species [‘Red Ruby’ grapefruit (Citrus paradisi) and ‘Valencia’ sweet orange (Citrus sinensis)] for 48 days in a greenhouse. To determine the irrigation requirements for the treatment 100%, we estimated the water loss from pots by calculating the difference in soil moisture between the day before and the day of the measurement. We irrigated the pots when the soil moisture was close to the maximum allowable water depletion, keeping the soil moisture between the field capacity and the maximum allowable depletion. A portable thermal camera was used to take images that were later analyzed using open-source software. We determined the canopy temperature, leaf photosynthesis and transpiration, and plant biomass. A positive relationship between the amount of water applied and the temperature response of plants exposed to different water levels was observed. Grapefruit and sweet orange plants that received less water presented water restrictions and reached 6 °C higher canopy temperatures than the air. The thermal images easily identified water-stressed plants. This study allowed quick measuring of the canopy temperature using readily available equipment and can be used as a tool to assess water status in citrus plants in greenhouses. An automated routine to process the thermal images in real-time and remove the background weeds to determine the canopy temperature can potentially allow using it for irrigation management.
Speakers
avatar for Gustavo Haddad Souza Vieira

Gustavo Haddad Souza Vieira

Full Professor, IFES campus Santa Teresa
Agronomist,Ph. D. in Agricultural Engineering. Full Professor. Irrigation, Agrometeorology, Horticulture, Coffee Crop.
Co-authors
RS

Rhuanito Soranz Ferrarezi

University of Georgia
NA
Wednesday September 25, 2024 11:15am - 11:30am HST
South Pacific 4

11:29am HST

Vegetable Crops Management 2 (VCM 2)
Wednesday September 25, 2024 11:29am - 1:10pm HST
Effects of Off-Season Winter Cover Crops and In-Season Nematicide Application on Plant-Parasitic Nematodes - Abolfazl Hajihassani
Lessons Learned from Living Mulch Trials on Midwest Vegetable Farms - Connor Ruen
Evaluating Cover Crop Biomass and Roller Crimper Technology for Effective Weed Control in Vegetable Systems - Arianna Bozzolo
Fall Cover Crops to Follow Carrot Harvest on High Organic Matter Soils - Mary Ruth McDonald
Cover Crops For Vegetable Research Farms Need Nitrogen - Thomas Bjorkman
Insurance Industry Standards Over-estimate Yield Loss Due to Stand Reduction in Processing Sweet Corn - Charlie Rohwer
Combining Lasers and Distress Calls to Control Birds in Sweet Corn - Rebecca Brown
Pea-oat Green Manure and Reduced Nitrogen Rate Delay Maturity of Broccoli But Do Not Reduce Yield - Charlie Rohwer
Field Screening of Cabbage (Brassica oleracea var. capitata) Cultivars for Resistance to Black Rot - Manisha Kumari
Evaluation of Brassica Oleracea Genotypes in Terms of Agronomic Performance - Sotirios Tasioulas
Moderator
avatar for Manisha Kumari

Manisha Kumari

Post-Doctoral Research Associate AD, The University of Georgia
Manisha Kumari is a post-doctoral research associate AD at Horticulture department, University of Georgia, Tifton campus, Tifton 31793, GA, USA.
Wednesday September 25, 2024 11:29am - 1:10pm HST
South Pacific 3

11:30am HST

VCM 2 - Effects of Off-Season Winter Cover Crops and In-Season Nematicide Application on Plant-Parasitic Nematodes
Wednesday September 25, 2024 11:30am - 11:40am HST
In the Southeast United States, control of parasitic nematodes in vegetable growing systems has traditionally relied on soil fumigation before planting vegetables. However, new regulations on the application of fumigant pesticides and concerns about their toxicity to non-target organisms are compelling growers to adopt sustainable alternatives. This study examined the effects of winter cover crops and subsequent production of cabbage treated with a non-fumigant nematicide on root-knot (Meloiodgyne incognita) and stubby-root (Nanidorus minor) nematodes. Off-season treatments consisted of oilseed radish cvs. “Control” and “Image”, cereal rye cv. “Wrerens Abrussi”, oat cv. “Tachiibuki”, black oat cv. “Protex” and mustard cv. “Caliente”. In-season treatments consisted of an untreated check and the nematicide fluensulfone, applied through the drip irrigation system one week before transplanting cabbage into raised beds covered with plastic mulch. A weedy fallow treatment was also included for comparison. After growing cover crops and incorporating plant residues into the soil, M. incognita population density was significantly reduced only in plots where Tachiibuki oat was grown. None of the cover crops reduced the population density of N. minor. Nematicide application on cabbage significantly reduced M. incognita populations in plots previously grown with Tachiibuki and Pratex oats, and mustard compared to the untreated check. Similarly, the nematicide reduced the N. minor population density in plots previously grown with oats, mustard, and rye. The efficacy of fluensulfone on root gall severity of cabbage was significant only in the untreated check, with plots previously grown with Image radish having a significantly lower root galling than the mustard, rye, and weedy fallow. Nematicide application reduced root gall severity of cabbage in plots previously grown with radish, Tachiibuki oat, and rye compared to the untreated check. Growing cabbage after cover crops and using the nematicide did not impact cabbage yield compared to the untreated check. These results indicate that cover cropping with Tachiibuki oat and the use of non-fumigant nematicides appear to be effective for managing these nematode species.
Speakers
AH

Abolfazl Hajihassani

University of Florida
Wednesday September 25, 2024 11:30am - 11:40am HST
South Pacific 3

11:30am HST

CHMG 1 - Enhancing Backyard Fruiting Cultivation Efficacy Through Florida-Friendly Landscaping™ Techniques for Homeowners
Wednesday September 25, 2024 11:30am - 11:45am HST
Florida's distinctive subtropical climate renders it a haven for the average backyard gardener. Yet, numerous homeowners grapple with challenges in cultivating dooryard fruits, often due to incorrect planting times or a dearth of expertise. Recognizing this, the University of Florida/Institute of Food & Agricultural Sciences (UF/IFAS) has introduced the Florida-Friendly Landscaping TM program, illuminating nine core principles. These guidelines equip homeowners with the necessary methods and techniques to optimize plant growth and development. As we continue to increase resident population daily from various parts of the world, educating homeowners on these principles, can significantly elevate their chances of realizing their cultivation aspirations.
To meet the persistent demand for edible landscapes, a series of enlightening workshops unfolded across Flagler, Putnam, and Volusia counties. These sessions honed in on sustainable management strategies tailored for cultivating delectable fruits like blackberries, mulberries, goji berries, and citrus varieties. Spanning two to three hours, participants delved into a comprehensive curriculum covering optimal planting and harvest timings, varietal nuances, cultivation methodologies, fertilizer selection, integrated pest management protocols, and the significance of hardiness zones. These workshops roamed through the tri-county region, shining a spotlight on local agricultural enterprises engaged in the production or promotion of sought-after backyard fruits.

This collaborative initiative not only bolstered our overarching educational goals but also catalyzed a surge in agrotourism intrigue. As each program reached its conclusion, attendees departed armed with two plants of their preference and a wealth of resources, poised to lay down the groundwork for thriving cultivation endeavors.
22 Program participants indicated the following as measured via post evaluation survey:
Participants indicated a 73% (n=16) implementation plan for Florida-Friendly Landscaping TM (FFL) principle of "Right Plant Right Place” as they continue in their gardening endeavors.
Participants indicated an 82% (n=18) knowledge gain on cultivar selection, soil testing, and importance of fertilization.
64% (n=14) of program participants shared a plan to continue backyard fruit production using the information disseminated during this program.
Speakers
BC

Brittany Council-Morton

UF/IFAS Volusia County Extension
Co-authors
CJ

Claude Judy Jean

University of Florida
Wednesday September 25, 2024 11:30am - 11:45am HST
South Pacific 2
  Oral presentation (Individual talk), Consumer Horticulture and Master Gardeners
  • SUBJECT Consumer Hort and Master Gardeners
  • Agricultural Sciences (UF/IFAS) 16) implementation plan for Florida-Friendly Landscaping TM (FFL) principle of "Right Plant Right Place” as they continue in their gardening endeavors. Participants indicated an 82% (n=18) knowledge gain on cultivar selection, soil testing, and importance of fertilization. 64% (n=14) of program participants shared a plan to continue backyard fruit production using the information disseminated during this program.

11:30am HST

HSMP 1 - Consumer preference of saffron uses in baked goods and dishes
Wednesday September 25, 2024 11:30am - 11:45am HST
Saffron (Crocus sativus L.) is a perennial cormous crop, possibly originated in Iran, cultivated in the Mediterranean climate region, including Iran, Afghanistan, Spain, Greece, and Kashmir. Due to the labor-intensive harvesting and processing involved, saffron is known as the most expensive spice. Its production has seen a revival in certain regions of the U.S., and Kentucky State University has been evaluating its potential as a niche crop for small and limited-resource farmers in Kentucky since 2019. One objective of this portion of the study was to evaluate consumer preferences for saffron used in various recipes. Two sets of sensory evaluation were conducted with saffron containing cheesecakes and baked goods (i.e., pound cake and short bread). There were three types of cheesecakes (vanilla, lemon, and persimmon) with and without saffron flakes. Overall, test subjects preferred cheesecakes without saffron, with the lemon cheesecake without saffron being the most popular combination. In contrast, participants showed preference for saffron containing recipes over the ones without saffron for the sensory evaluation of both baked goods, especially shortbread. A preliminary test for taste testing was conducted with pound cake to determine the optimal content of saffron. There were three levels of saffron, which was first dissolved in water, and then added to mixture, for both pound cake and shortbread (0, 0.5 and 1.0 tbsp/recipe). The current findings suggest that consumers are favorable of baked goods when saffron is compatible and used in the correct amount and possibly in the correct form.
Speakers
HK

Hideka Kobayashi

Kentucky State University
Co-authors
SC

Sheri Crabtree

Kentucky State University
Wednesday September 25, 2024 11:30am - 11:45am HST
South Pacific 1

11:30am HST

O/LT 1 - The Effects of Mulch Color and Depth on Soil Temperature and Light Transmission
Wednesday September 25, 2024 11:30am - 11:45am HST
Mulching is a common task in the landscape industry, with materials selected to provide environmental benefits (i.e. moderating soil conditions, limiting weed growth) and aesthetic value, with colored mulches often employed to add an artistic element to landscapes. Questions arise over possible effects that mulch color may have on soil temperatures, especially when using darker materials. This research investigated the effects of a commercially available shredded mulch (dyed black, brown, or red) on soil temperature and light transmission in model research plots. A plot at the Hammond Research Station was cleared, graded, and prepared with a typical bed mix comprised of pine bark and sand. A total of 21 sub-plots were prepared, where each sub-plot had a remote temperature sensor buried at the base of the bed mix (8 cm below surface), and a temperature and light sensor placed over top of the bed mix. Mulch was applied to depths of 5 cm or 10 cm directly over the top of the temperature and light sensors, with n=3 for our control (no mulch over the bed mix), red mulch (n=3 for depth of 5 cm and n=3 for depth of 10 cm), brown mulch (n=3 for depth of 5 cm and n=3 for depth of 10 cm), and black mulch (n=3 for depth of 5 cm and n=3 for depth of 10 cm). Soil temperature conditions (both within the mulch itself, and at the base of the bed mix) as well as light transmission through the mulch layer was recorded every 30 minutes throughout a spring and summer season at the Hammond Research Station. Blank (unmulched) plots naturally experienced the most light transmission and temperature extremes. Regardless of mulch color or depth, light transmission was substantially reduced (and often eliminated) equivalently between mulch treatments. Temperature was measured both within the surface mulch layer, and 8 cm below into the subsurface bedding mix. While subsurface temperatures were effectively equivalent between all mulched plots, surface temperatures exhibited substantial differences between mulch colors and depths. Thinner mulch layers experienced more extreme surface temperature fluctuations, with mulch color influencing peak temperatures. The results of this work suggest that different mulch colors and depths have a greater influence on temperature at the immediate surface, but far more muted differences in subsurface temperatures.
Speakers
DA

Damon Abdi

Louisiana State University Agricultural Center
Co-authors
AH

Ashley Hickman

Louisiana State University Agricultural Center Hammond Research Station
NA
JF

Jeb Fields

LSU AgCenter Hammond Research Station
Wednesday September 25, 2024 11:30am - 11:45am HST
Coral 1

11:30am HST

PH 2 - Improving Postharvest Storage and Late-Season Production of Caladium Tubers in Florida Through Chemical Treatments
Wednesday September 25, 2024 11:30am - 11:45am HST
Florida is home to the largest caladium production in the world, supplying essentially all the global caladium tuber demand. These plants are famous for their vibrant leaf colors and patterns and are asexually propagated through tubers. Following plant development, tubers are harvested and stored for a few months before being forced from March to September for potted plant production and direct landscape planting. During extended postharvest storage, caladium tubers risk severe weight loss, tissue decay, and Fusarium tuber rot. The current storage practices under ambient conditions with high temperatures and high relative humidity exacerbate weight loss and tuber rot. The challenges of long-term tuber storage make it difficult to produce pot caladium plants for winter holidays, including Thanksgiving, Christmas, and New Year. These challenges also affect the commercialization of tubers in the Southern Hemisphere during the summer. Opening these marketing opportunities can allow growers to extend and increase their production. Additionally, identifying caladium tubers suitable for late-season production will give Florida growers a competitive advantage. To identify caladium varieties with long-term tuber storage potential and late-season production, 12 varieties were evaluated to target Thanksgiving and Valentine’s Day. No.1-sized tubers were potted in 5-inch containers and grown in a greenhouse. For each variety, 10 tubers were monitored for sprouting and leaf expansion and later evaluated for plant quality using a rating scale from 1 to 5. Potted tubers from all cultivars achieved suitable market quality standards when targeting Thanksgiving, with the higher ratings obtained by cultivars ‘Splash of Wine’, ‘Classic Pink’, and ‘Lemon Blush’. The higher number of leaves and height were obtained by cultivars ‘Classic Pink’, ‘Desert Sunset’, and ‘White Wonder’, and ‘Party Punch’, ‘Ballet Slipper’, and ‘Classic Pink’, respectively. For Valentine’s Day, despite sprouting decreasing, cultivars ‘Splash of Wine’, ‘Hot 2 Trot’, ‘Desert Sunset’, ‘Party Punch’, ‘Classic Pink’, ‘White Wonder’, and ‘Lemon Blush’ reached suitable market quality standards. Overall, the number of leaves and height decreased compared to plants targeting Thanksgiving, however, cultivars with a more desirable number of leaves were ‘Classic Pink’ and ‘Lemon Blush’ whereas cultivars with higher height were ‘Party Punch’ and ‘Ballet Slipper’. Identifying cultivars suitable for late-season production will allow growers to expand their commercialization window for major holidays and to supply tuber demand to the Southern Hemisphere.
Speakers
GC

Gasselle Cordova

University of Florida
Co-authors
ZD

Zhanao Deng

University of Florida
Wednesday September 25, 2024 11:30am - 11:45am HST
Nautilus

11:30am HST

UG - The Assessment of Different Growth Mediums for Plug Cultivation in a Controlled Environment
Wednesday September 25, 2024 11:30am - 11:45am HST
Plugs are crucial for starting crop production in greenhouses, soil, and controlled environment agriculture (CEA). Horticultural, vegetable, fruiting, and ornamental crops that utilize plugs for production have demonstrated better plant health, transplant establishment rate, and total yield. Many substances are capable of supporting plug growth, so the APS Laboratory for Sustainable Food at Florida Gulf Coast University investigated the quality of plugs prepared based on different commonly used growth mediums for plug production. We carried out the growth of Rex Butterhead Lettuce Latuca Sativa plugs with six different treatments: 1) Rockwool, 2) Oasis® Horticube, 3) Perlite 4) Coco Coir, 5) Phenolic Foam, and 6) Peat Pellets. The seeds were sowed in their respective growth medium and watered every day. The plugs were then cultivated for 15 days in a controlled environment until two leaves apart from the cotyledon had developed. After 15 days, we collected data which included wet weight (g), dry weight (g), leaf area (cm2), nitrogen content (mg/g), and chlorophyll concentration (mg/cm2). In addition, we derived data including the Leaf Area Index (LAI) and Specific Leaf Area (SLA, cm2/g). Descriptive statistics were used to describe the biomass data. Pairwise permanovas were conducted, followed by pairwise Wilcoxon tests to determine which treatments result in significant differences for each response variable. A permutation MANOVA revealed a significant treatment effect on plug preparation (p=0.001). All subsequent multilevel pairwise comparisons were significant, with the exception of phenolic foam vs perlite (p=0.294). Of all the treatments, we concluded that plugs grown in Peat Pellets produced the most viable plugs with the largest wet weight (g), dry weight (g), and total leaf area (cm2). Results from this study may inform growers about appropriate growth mediums for efficient plug production. Keywords: Controlled Environments, Growth Mediums, Lettuce, Plugs, Urban Agriculture
Speakers
avatar for George Buss

George Buss

Florida Gulf Coast University
Co-authors
AS

Ankit Singh

University of Maine
NA
GP

Galen Papkov

Florida Gulf Coast University
NA
JL

John L Griffis

Florida Gulf Coast University
NA
KJ

Kathryn Jackson

Independent Researcher
NA
MG

Mya Griffith

Florida Gulf Coast University
PC

Paige Carroll

Florida Gulf Coast University
NA
SB

Sarah Bauer

Mercer University
NA
XY

Xiusheng Yang

University of Connecticut
NA
Wednesday September 25, 2024 11:30am - 11:45am HST
Lehua Suite

11:30am HST

WUM 1 - The Impact of Irrigation Rates Based on Crop Water Requirement on Tree Growth and Water Relations in Commercial Citrus Groves
Wednesday September 25, 2024 11:30am - 11:45am HST
The growing demand for affordable and healthy food to feed the growing population necessitates multilayered strategies to meet food demand and supply features: excessive irrigation application to overcome the impact of erratic rainfall, which imposes pressure on groundwater withdrawals, adversely affecting crop failure and sustainability. The objective of the study was to determine the impact of varying irrigation levels on tree growth, leaf nutrient concentrations, and water relations at selected citrus tree densities. The experiment was carried out on Malabar fine sand (sandy, siliceous, hyperthermic Arenic Alaquods) in a commercial citrus grove near Immokalee, FL, USA from 2019 to 2022. Mature thirteen-year-old ‘Valencia’ (Citrus sinensis) citrus trees grafted on Carrizo (a hybrid of Washington Navel orange and Poncirus trifoliata) planted in tree densities of 360, 485, and US-897 (Citrus reticulata Blanco x Poncirus trifoliata (L.) Raf.) citrus rootstock with 920 trees ha-1. Significant water distribution and movement were detected along the soil profile in response to the irrigation rates with higher volumetric water content on the grower standard highest irrigation. As a result, significant fibrous root length densities (FRLD) and median lifespan were observed in the three-row and two-row experiments with the deficit (50%-crop evapotranspiration, ETc) and moderate (78%-ETc) as compared with the grower standard highest (100%-ETc) irrigation regimes, respectively. Stomata conductance and stem water potential ( manifested less tree water stress when trees received moderate irrigation in the low and moderate tree densities than the highest tree density. This significantly impacted the FRLD in the soil and leaf area index (LAI) above the ground tree growth. Moderate irrigation triggered FRLD and improved root survival probability and lifespan. Meanwhile, nutrient uptake from the soil significantly affected leaf nutrient concentration when trees received moderate irrigation than deficit or highest irrigation rates. As a result, irrigation management improved water relations, leaf nutrient concentration, and tree growth across the varying irrigation regimes.
Speakers
AA

Alisheikh Atta

University of Florida
Alisheikh Atta currently works at the Department of Soil, Water, and Ecosystem Science, University of Florida. Alisheikh does research on HLB-affected citrus nutrient and water management. He studies the impact of plant nutrients on the performance of citrus trees, irrigation water... Read More →
Co-authors
DK

Davie Kadyampakeni

University of Florida
NA
KM

Kelly Morgan

University of Florida
NA
SH

Said Hamido

Rodale Institute
Soil Scientist
Wednesday September 25, 2024 11:30am - 11:45am HST
South Pacific 4

11:30am HST

Dr. Liberty Hyde Bailey's First ASHS Presidential Address
Wednesday September 25, 2024 11:30am - 12:00pm HST
Speakers
avatar for Curt Rom

Curt Rom

University Professor. And Associate Director, University of Arkansas and ASHS
Wednesday September 25, 2024 11:30am - 12:00pm HST
Coral 5 - ASHS Hort Theater

11:40am HST

VCM 2 - Lessons Learned from Living Mulch Trials on Midwest Vegetable Farms
Wednesday September 25, 2024 11:40am - 11:50am HST
In the Great Plains region of the United States, perennial clover living mulch is being explored for the potential benefits of reducing tillage, suppressing weeds, and supplementing soil. Perennial living mulch systems also have the potential to reduce the use of single-use plastic. Research on the use of perennial living mulch in vegetable systems has been limited in the Great Plans, and farmers have expressed interest in trialing this system to improve soil health and reduce erosion by wind and rain. Two vegetable farms, Haroldson Farms in Bruce, SD and Blue Sky Vegetable Company in Worthing, SD, have participated in the on-farm trials since 2023. In collaboration with South Dakota State University, the on-farm trial collaborators were interested in using living mulch to control weeds and reduce the need for plastic. ‘Domino’ white clover (WC) (Trifolium repens), and ‘Dynamite’ red clover (RC) (Trifolium pratense) are the clovers that were selected for the on-farm trials. The clover struggled to grow with the lack of rainfall and was soon overtaken by weeds. Results quickly showed that the lack of moisture had a detrimental effect on the clover’s ability to compete and establish in walkways. A mixture of grasses and broadleaf were observed as the clover declined. Early moisture is essential for the establishment of clover living mulch and be used to its full potential. The planting method of frost seeding would be recommended for future trials to encourage clover germination and establishment before weeds get large enough to compete.
Speakers
CR

Connor Ruen

South Dakota State University
NA
Co-authors
KL

Kristine Lang

South Dakota State University
TN

Thandiwe Nleya

South Dakota State University
NA
Wednesday September 25, 2024 11:40am - 11:50am HST
South Pacific 3

11:44am HST

Fruit Breeding (FRBR)
Wednesday September 25, 2024 11:44am - 12:45pm HST
Haplotype-Phased Genome Assemblies of ‘Sulhyang’ Strawberry (Fragaria ×ananassa) using PacBio Revio Sequencer - Youngjae Oh
Exploring the molecular basis of rootstock-mediated growth vigour in macadamia: Unravelling aquaporin gene dynamics - Pragya Dhakal Poudel
Multivariate Analysis of Fruit Composition Diversity Among the North Carolina Strawberry Germplasm Collection - Gina Fernandez
Improving Blueberry By Dissecting Fruit Texture - juan david jimenez pardo
Assessment of Fruit Composition and its Influence on Flavor and Consumer Preferences in Blackberry - Katelyn Lust-Moore
Sugar Content and Gene Expression Dynamics in Apple Spurs in Response to Fruit Load and Gibberellins - Qiuyi Fu
A Census and The Conservation of Malus coronaria in Michigan - Lily Christian

Moderator
BH

Brianna Haynes

North Carolina State University
Wednesday September 25, 2024 11:44am - 12:45pm HST
Kahili

11:45am HST

FRBR - Haplotype-Phased Genome Assemblies of ‘Sulhyang’ Strawberry (Fragaria ×ananassa) using PacBio Revio Sequencer
Wednesday September 25, 2024 11:45am - 11:55am HST
Cultivated strawberry (Fragaria ×ananassa Duch, 2n = 8× = 56) belongs to the family Rosaceae and is an allo-octoploid species. We report here a high-quality haplotype-phased genome assembly of a representative Korean short-day cultivar, ‘Sulhyang’ using a trio-binning approach. Using PacBio Revio long reads of ‘Sulhyang’ and whole genome paired-end reads (Illumina) of two parents ‘Akihime’, and ‘Yukbo’, we completed telomere-to-telomere phased genome assemblies of both haplotypes. The ‘Sulhyang’ genome was 797 Mb , with a long terminal repeat (LTR) assembly index (LAI) of 17.28. The genome was phased into two haplotypes, phase-1 (784.9 Mb with contig N50 of 12.34) and phase-2 (781 Mb with contig N50 of 16.04 Mb). The BUSCO analysis detected over 99% of conserved genes in the combined phased-1 and phased-2 assembly. Both haploid assemblies were annotated using RNA-Seq data representing various F. ×ananassa tissues from the NCBI sequence read archive, resulting in a total of 104,099 genes in the primary assembly. This haplotype-phased reference genome for ‘Sulhyang’ will broaden our knowledge of strawberry genome evolution and gene functions.
Speakers
avatar for Youngjae Oh

Youngjae Oh

Prof., Chungbuk National University
Co-authors
HH

Hyeondae Han

University of Florida/IFAS/Gulf Coast REC
NA
SL

Seonghee Lee

University of Florida/IFAS/Gulf Coast REC
NA
Wednesday September 25, 2024 11:45am - 11:55am HST
Kahili

11:45am HST

O/LT 1 - Subterranean Termite Landscape Mulch Consumption Challenge
Wednesday September 25, 2024 11:45am - 12:00pm HST
Formosan Sub-terranean Termite Landscape Mulch Consumption Payton Floyed1, Edward Bush*2, and Qian Sun1 (1)LSU Department of Entomology and (2)LSU AgCenter, SPESS, Baton Rouge, LA Many landscapers utilize organic mulch substrates composed primarily of wood and bark, making it an ideal food source for the Formosan subterranean termite (Coptotermes formosanus). Formosan termites are one of the most destructive structural pests and recognized as one of the 100 worst invasive species in the world. While foraging, these termites can find and may be able to fully establish colonies in landscaping that uses mulch. The mulch type that attracts the most termites has not been widely investigated and continues to be an issue that needs to be determined. The objective of this research was to measure the biomass consumption by termites. Three C. formosanus colonies were used, two from New Orleans, Louisiana, and one from Gonzales, Louisiana. All were maintained in the laboratory using three total replications per experiment over a 14 d period. Five-hundred total termites (450 workers and 50 soldiers) were placed in each arena (7.5”x10”x4” plastic bin) which used a sand layered bottom for both worker and soldier termites. Each arena was covered with a dark plastic bag to mimic typical subterranean foraging conditions. Mulch particle size distribution and bulk density resulted in expected differences with crushed pine straw having the finest particle size (>50% particle size
Speakers
EB

Edward Bush

LSU AgCenter
Co-authors
QS

Qian Sun

LSU AgCenter
NA
Wednesday September 25, 2024 11:45am - 12:00pm HST
Coral 1

11:45am HST

PH 2 - Impact of Storage Environment on Dormancy Progression and Microbiome of Potato Tubers
Wednesday September 25, 2024 11:45am - 12:00pm HST
Preservation of nutritional and market qualities of potato tubers during postharvest storage is essential for optimum economic return. Protecting tubers from unintended sprouting during storage is one of the major postharvest challenges that potato growers and stakeholders encounter. Storage environment, particularly storage temperature, significantly impacts dormancy progression and sprouting and effect overall marketability. Potato tubers carry significant amount of microbiome in different tissues of tubers from field to storage, which might contribute to overall storage qualities. However, how storage temperature impacts overall potato tuber microbiome and how changes in microbiome influence tuber dormancy are largely unknown. Therefore, the primary objective of this study was to investigate the impact of different storage temperatures on tuber microbiome and monitor the dormancy progression and sprout growth during long-term storage. Certified seed tubers of Russet Burbank were cured after harvest following industry practices. Then, one subset of tubers was stored at constant 8°C, and a second subset was stored at constant 21°C. Three types of tuber tissues (primary meristem, secondary meristem, and tuber flesh) were collected after 4, 8, 12, 17, and 24 weeks of storage for microbiome profiling and sugar and protein analysis. Additionally, a third subset was generated by moving tubers from 8°C to 21°C three days before sampling time of 8, 12, and 17 weeks. Tuber dormancy progression was monitored using additional subsets for each storage condition. As expected, the subset of tubers stored at 8°C had delayed sprouting compare to 21°C, and rapid sprout growth was observed when tubers were moved from 8°C to 21°C. Increase in reducing sugar content was observed in meristem tissues, while it decreased in tuber flesh with progression of storage duration. Interestingly, higher protein content was determined in secondary meristem tissues compare to primary meristems and tuber flesh. Overall, greater bacterial and fungal diversity and abundance were observed in meristem tissues when compared to tuber flesh. Additionally, storage temperatures, storage time, and tuber tissue types significantly impacted tuber microbial profile. In tuber tissues, Ascomycota and Basidiomycota were predominant fungal phyla, while Actinobacteria, Proteobacteria, and Cyanobacteria were the predominant bacterial phyla. Our results suggested that storage temperature and storage duration significantly impact both dormancy progression and tuber microbiome and subsequently contribute to postharvest qualities of potato tubers.
Speakers
avatar for Munevver Dogramaci

Munevver Dogramaci

United States Department of Agriculture
Co-authors
DS

Dipayan Sarkar

United States Department of Agriculture
NA
EF

Evandro Fortini

United States Department of Agriculture
NA
MB

Malick Bill

United States Department of Agriculture
NA
SK

Shyam Kandel

United States Department of Agriculture
NA
Wednesday September 25, 2024 11:45am - 12:00pm HST
Nautilus

11:45am HST

UG - Comparative Analysis of Lettuce Growth Using Compost Versus Conventional Soil
Wednesday September 25, 2024 11:45am - 12:00pm HST
 Conventional agricultural techniques have been degrading American soils nationwide since the beginnings of modern-day agriculture through practices such as soil tilling, using nitrogen synthetic fertilizers, and monocultural systems. These previously mentioned techniques contribute to degrading soil health, mass emissions of carbon dioxide into the atmosphere, and decreased biodiversity. Regenerative agriculture offers a combination of sustainable practices that will create carbon sinks to sequester atmospheric carbon dioxide, restore national food systems, and prioritize soil health. Regenerative agriculture techniques include the utilization of cover crops, compost, no-tillage, mob grazing, and polyculture. The APS Laboratory for Sustainable Agriculture focused on the effectiveness of compost by comparing the growth of lettuce in four different treatments: 100% compost (100%C), 75% compost 25% Miracle-Gro (75%C-25%MG), 50% compost 50% Miracle-Gro (50%C-50%MG), and finally, 100% Miracle-Gro (100%MG). The lettuce seeds were kept in a growth tent for 15 days during their period of germination before being transferred to four 1x1 meter plots in the Food Forest at Florida Gulf Coast University (FGCU) for the 60-day growth period. The lettuce crops grew to full bloom and ready for harvest. Sampling events took place every six days in which crop growth data including wet weight (g), dry weight (g), chlorophyll concentration (μmol/m^2), and leaf area (cm^2) were collected. Specific Leaf Area (g/cm^2) and Leaf Area Index were derived, and statistical analysis was conducted. Based on the statistical tests conducted at the 5% significance level using R statistical software, soil treatment type was found to be significant (p=0.0002). Soil treatment type was shown to have significantly impacted wet weight (p<0.0001), dry weight (p<0.0001), and leaf area (p=0.0011), but not nitrogen (χ^2 [3]=3.91, p=0.2717). Results demonstrate the effectiveness and feasibility of using compost as a technique for regenerative agriculture.
Speakers
SH

Sofia Huber

Florida Gulf Coast University
Co-authors
AS

Ankit Singh

University of Maine
NA
GP

Galen Papkov

Florida Gulf Coast University
NA
JG

John Griffis

Florida Gulf Coast University
NA
KJ

Kathryn Jackson

Independent Researcher
NA
SB

Sarah Bauer

Mercer University
NA
XY

Xiusheng Yang

University of Connecticut
NA
Wednesday September 25, 2024 11:45am - 12:00pm HST
Lehua Suite

11:50am HST

VCM 2 - Evaluating Cover Crop Biomass and Roller Crimper Technology for Effective Weed Control in Vegetable Systems
Wednesday September 25, 2024 11:50am - 12:00pm HST
This study aims to evaluate the efficacy of roller crimper technology in tandem with various cover crop mixtures for weed management in coastal California's vegetable systems. The experiment was conducted at the Rodale Institute California Organic Center (34.220453, -199.108214) in Camarillo, CA. Twenty randomized plots 46 x 12 m were measured and assigned to either to a bare soil treatment or one of two cover crop mixes: oat (Avena sativa) hairy vetch (Vicia villosa), or oat pea (Pisum sativum), with cover crops terminated via conventional tillage using a disk (T) or using a roller crimper for no-till system (NT). Pumpkins (Howden variety) were direct seeded, and data on cover crop growth and weed pressure were collected at three time points: before cover crop termination, after cover crop termination and after harvest. At harvest, a subset of each plot was sampled to count and weigh pumpkins for crop yield assessment. Before termination, the total dry matter of cover crop biomass showed no significant differences between oat/peas and oat/vetch mixes. Oat/vetch exhibited lower weed biomass at 0.74 t/ha compared to oat/peas at 1.98 t/ha (0.8 t/a); bare soil plots had the greatest biomass at 21.35 t/ha. After termination (30 DAT), NT plots showed similar biomass production between oat/peas and oat/vetch. T plots displayed residues on the soil surface, with higher amounts in oat/vetch at 8.7 t/ha compared to oat/peas at 8.4 t/ha. Weed emergence after cover crop termination was comparable across all plots with cover crops, ranging from 1.24 t/ha to 2.22 t/ha, while bare soil exhibited higher weed biomass at 10 t/ha compared to plots with cover crops. After harvest (120 DAT), oat/vetch in NT plots showed greater biomass retention compared to T plots, as did oat/peas in NT plots. All plots experienced biomass reductions, with oat/peas T plots experiencing the most significant decrease at 61.5%. Oat/vetch mixture plots (T and NT) and bare soil had similar pumpkins production per hectare, outperforming oat/pea T plots in terms of fruit number and production per hectare. Oat/pea T plots exhibited decreased fruit weight compared to bare soil.
Speakers
avatar for Arianna Bozzolo

Arianna Bozzolo

Rodale Institute
Co-authors
AS

Andrew Smith

Rodale Institute
NA
JP

Jacob Pecenka

Rodale Institute
NA
Wednesday September 25, 2024 11:50am - 12:00pm HST
South Pacific 3

11:55am HST

FRBR - Exploring the molecular basis of rootstock-mediated growth vigour in macadamia: Unravelling aquaporin gene dynamics
Wednesday September 25, 2024 11:55am - 12:05pm HST
Rootstocks play a crucial role in shaping plant growth and vigour, driven by a complex interplay of physiological, molecular, and genetic factors. Aquaporins (AQPs), as key water transport proteins, are emerging as pivotal component in understanding rootstock-mediated vigour. However, evidence for rootstock effects in macadamia remains limited with no exploration into the mechanisms of rootstock-mediated vigour control, hindering progress in rootstock development for this crop. In this study, we aimed to explore the role of AQPs by conducting a genome-wide investigation on whole genome sequence of Macadamia integrifolia (‘HAES741’). This study identified 37 putative macadamia AQP (miAQP) genes belonging to 5 different sub-families including 11 plasma membrane intrinsic proteins, 10 tonoplast intrinsic proteins, 10 Nod26-like intrinsic proteins, 2 small and basic proteins and 6 X-intrinsic proteins. miAQP genes are unevenly distributed across 11 chromosomes with chromosome 7 possessing the most genes. To investigate the differential expression of plasma membrane intrinsic proteins (PIPs), in high- and low-vigour rootstocks, root, leaf, and stem tissues were collected from trees with a single scion ‘HAES741’ grafted on to 7 different rootstock genotypes . RNA from tissue samples were extracted by a modified CTAB RNA extraction protocol using the Zymo-spin™ IICR Column. RNA quality and quantity was checked via nano spectrophotometer and gel electrophoresis. Primer pairs for 11 PIP genes and 2 housekeeping genes (ACT and EF1b) were designed using Primer BLAST in NCBI. RNA samples were subjected to a real time quantitative RT-PCR. The tissue specific expression data will be analysed to observe differential expressions, if any, between high and low vigour rootstocks and scions. Differential expression patterns reveal the PIP genes associated with vigour control. The correlation between gene expression levels and rootstock vigour provides better understanding on the intrinsic molecular mechanism vigour regulation in macadamia. Moreover, comparative analysis of PIP gene expression between rootstock and scion can provide insights into the rootstock-scion interactions. Overall, results from this study offer a better understanding of the molecular basis of rootstock-mediated vigour control with implications for rootstocks development and orchard optimization in macadamia.
Speakers
avatar for Pragya Dhakal Poudel

Pragya Dhakal Poudel

The University of Queensland
Co-authors
BA

Borhan Ahmed

Bangladesh Jute Research Institute
NA
BT

Bruce Topp

Queensland Alliance for Agriculture and Food Innovation, The University of Queensland
NA
LS

Lindsay Shaw

Queensland Alliance for Agriculture and Food Innovation, The University of Queensland
NA
MA

Mobashwer Alam

Queensland Alliance for Agriculture and Food Innovation, The University of Queensland
NA
Wednesday September 25, 2024 11:55am - 12:05pm HST
Kahili

11:59am HST

Herbs, Spices, and Medicinal Plants (HSMP)
Wednesday September 25, 2024 11:59am - 1:10pm HST
Effects of Drought Stress on Secondary Metabolite Production and Yield in Ocimum Basilicum - Daniela Menendez
Effects of Thermal Processing and Drying on Nutritional Quality and Consumer Preference of Amaranthus viridis L. Value-added Product - Megan Reid
Ginseng Growth and Ginsenoside Contents According to Light Intensity - Minhee Kim
Evaluation of anticancer activity of perennial tree seed-derived extracts on various cancer cell lines - Hamin Lee
Exploring the Potential of Annonacin and 2-Deoxy-D-glucose on Non-Small Cell Lung Cancer Cells - Bhoj Raj Bhattarai
Responses and relationship between bioactive compounds and phenotypic traits in Dahurian angelica breeding lines - Eun Jeong Koh
Effects of Foliar Application of Humic Acid and NPK Fertilizers to the Soil on Important Growth Indices of Black Mustard - Keefah Al-Garallaa
Assessment of micropropagated ginger cultivars and generations on plant growth and rhizome yield in a greenhouse - Guochen Yang

Moderator
KA

Keefah AL-Garallaa

Mississippi State University
Wednesday September 25, 2024 11:59am - 1:10pm HST
South Pacific 2

12:00pm HST

HSMP - Effects of Drought Stress on Secondary Metabolite Production and Yield in Ocimum Basilicum
Wednesday September 25, 2024 12:00pm - 12:10pm HST
Climate change makes water stress a more prominent obstacle to maintaining the same yield and quality of harvestable crop biomass for consumers, posing a unique challenge to farmers who must compete for clean water resources with other stakeholders. Thus, farmers must adapt their irrigation strategies to remain profitable and relevant. Basil is a tender perennial crop from the family Lamiaceae that is grown as a culinary herb and as a source of essential oils. Sweet basil (Ocimum basilicum) is a model crop for understanding water stress in economically important crops due to its intolerance to drought stress and its unique phytochemical properties. Sweet basil produces different concentrations of different secondary metabolites in response to abiotic stress, such as eugenol and methyl chavicol. This study seeks to understand what chemical and physical changes occur to sweet basil under water stress within a greenhouse environment. Three different treatments of water stress controlled via drip irrigation and a separate control group were used to analyze the yield and secondary metabolite production within sweet basil. Wet weight, dry weight, height, and phytochemical concentrations were calculated for the different drought treatments. There was a significant relationship between the final height, dry and wet yield, and chemical concentration of the different water stress treatments. One of the most interesting findings of the study was that the high note of sweet basil, a phenylpropanoid known as eugenol, was found in similarly high concentrations throughout the different treatment groups. There were significant differences in concentration across terpenoids such as eucalyptol, germacrene D, and linalool. This study showed that there were significant effects on the yield and concentration of phytochemicals produced by sweet basil from drought stress.
Speakers
DM

Daniela Menendez

Florida International University
Co-authors
DS

Diego Salazar Amoretti

Binghamton University
NA
KJ

Krishnaswamy Jayachandran Jayachandran

Florida International University
NA
MB

Mahadev Bhat

Florida International University
NA
Wednesday September 25, 2024 12:00pm - 12:10pm HST
South Pacific 2

12:00pm HST

VCM 2 - Fall Cover Crops to Follow Carrot Harvest on High Organic Matter Soils
Wednesday September 25, 2024 12:00pm - 12:10pm HST
The high organic matter (muck) soils in the Holland Marsh, Ontario, Canada, are prone to wind erosion, especially in the fall and winter months. Good canopy coverage of ideally 30%, is important for protecting the soil and reducing soil erosion. Carrots are usually harvested in October. Cool temperatures at this time result in slow germination and growth of cover crop species. Field trials were conducted to assess the efficacy of various cover crops and methods for rapid establishment after carrot harvest. One approach to increasing germination and emergence is seed priming. Seeds of barley, oats, and triticale were primed by soaking for 24 hr in water (hydro-priming) or potassium nitrate at 5 mg L-1 (osmo-priming). Seed was dried for 24 hours and then seeded into pots placed in controlled environments at 5, 10 or 21°C. Priming generally resulted in faster germination and higher biomass especially at lower temperatures of 5 and 10°C. Primed and non-primed seed was seeded into high organic matter soil (50% organic matter, pH 6.9) on 17 Oct. and crop growth was assessed on 13 Nov. There were no advantages of priming in the field trials. Non-primed barley had high or higher canopy coverage (13%), plant counts and dry weight, compared to other species and primed seed. Barely was also grown as transplants with 2 seeds/plug and transplanted in the trial on 17 Oct. This treatment had higher canopy coverage (25%) and dry weight than seeded treatments. However, using barley transplants is not a cost effective option at this time. In a separate trial on an adjacent site, barley was over-seeded into standing carrots on 27 Sept. at 200 kg ha-1, and the carrots were harvested on 17 Oct. Barley and fall rye were direct seeded on 17 Oct. Pre-harvest seeding of barley resulted in significantly better establishment, canopy coverage and biomass, although all were low, maximum 12% coverage. There was a strong positive correlation between canopy coverage and biomass production across all trials. More research is needed on cover crops species and establishment methods.
Speakers
avatar for Mary Ruth McDonald

Mary Ruth McDonald

Professor, University of Guelph
Research and extension interests include vegetable production and crop protection, with the emphasis on onions, carrots, celery and Brassica crops. Also conducting research on soil health of high organic matter soils and agricultural robots.
Co-authors
KS

Kimberly Schneider

University of Guelph
NA
NP

Neem Pandey

University of Guelph
NA
Wednesday September 25, 2024 12:00pm - 12:10pm HST
South Pacific 3

12:00pm HST

PH 2 - Strawberry Anthocyanins, Extraction, Assessments and Storage
Wednesday September 25, 2024 12:00pm - 12:15pm HST
The anthocyanin pigments have antioxidant activities and play critical roles in plant and human health. They are abundant in flowers, vegetables, and fruits and are soluble in water, methanol, and nonpolar solvents such as chloroform. Extracting stable pigments with higher concentrations has been the research community's and industries' goal. Spectrophotometric methods can easily measure the total anthocyanin content. Other complex and more expensive methods, such as high-pressure liquid chromatography and ultrasound-assisted methods, are also available to identify and quantify the anthocyanin pigment. Strawberry color affects the appearance, fruit quality after harvest, and consumers' decision-making process. A large group of factors, such as sample type, temperature, pH, solvent type, and the ratio of its components, affect strawberry anthocyanin yield. We studied the effect of some of these variables on the anthocyanin yield, profile, and color of strawberries. Extraction solvents significantly changed the anthocyanin yield. The results suggest that acidified chloroform-methanol extracted the highest anthocyanin content compared to water-based solvents. Methanol-water-based solvents also performed better than water alone. Processing time (incubation time) was lowest in the pH differential method; however, the haze produced in this method may interfere with the spectrophotometry. Chloroform-methanol solvent with higher pH extracted pelargonidin as the main anthocyanin, and methanol and water-based solvents extracted delphinidin in UHPLC. Chloroform extracts reduced the redness and increased the extracts' brightness mainly due to higher pelargonidin content. Less redness and increased brightness indicated some level of color degradation of the extracts after storage for 48h at 4 °C.
Speakers
Wednesday September 25, 2024 12:00pm - 12:15pm HST
Nautilus

12:00pm HST

UG - Feasibility of Plug Production Utilizing Digestate From Home Water-Energy-Food Systems (H-WEF)
Wednesday September 25, 2024 12:00pm - 12:15pm HST
The integration of sustainable technologies in waste management systems has become imperative in addressing the escalating challenges of agricultural productivity and sustainability. Plugs are essential when starting crop production in controlled environment agriculture (CEA) setups and greenhouses. Horticultural crops such as vegetables, fruiting, and ornamental plants that utilize plugs have demonstrated higher success rates, healthier plants, and higher total yields. The APS Laboratory for Sustainable Agriculture at explored the innovative utilization of digestate from the Home Water-Energy-Food Systems (H-WEF), the H-WEF system converts household food waste into biogas, electricity, and nutrient-rich digestate. The digestate from the H-WEF system was used to produce agricultural plugs, presenting a novel approach to circular resource utilization. We carried out the growth of Rex Butterhead Lettuce Latuca Sativa plugs with eight different treatments, 1) control synthetic fertilizer; 2) 5% Digestate – 95% RO Water (5D–95RO); 3) 10% Digestate – 90% RO Water (10D–90RO); 4) 15% Digestate – 85% RO Water (15D–85RO); 5) 20% Digestate – 80% RO Water (20D–80RO); 6) 25% Digestate – 75% RO Water (25D–75RO); 7) 30% Digestate – 70% RO Water (30D–70RO); 8) 35% Digestate – 65% RO Water (35D–65RO). The seeds were sowed with their fertigation treatment and watered every day. The plugs were cultivated for 15 days in a controlled environment until two leaves had developed after the cotyledon. After 15 days, we collected data on wet weight (g), plug head area (cm2), total leaf area (cm2), total nitrogen content (mg/g), total chlorophyll content (mg/cm2), and dry weight (g). In addition, we collected data on the Leaf Area Index (LAI, cm2/cm2) and Specific Leaf Area (SLA, cm2/g). The synthetic fertigation yielded a higher wet weight than the following treatments: 5D–95RO, 10D–90RO, and 35D–65RO. While the 30D–70RO treatment produced a larger plug head than all other treatments. The digestate-based fertilizers were comparable to the synthetic fertilizer at dilutions of 25D–75RO and 30D–70RO. Results from this study may inform growers about the viability of utilizing digestate for plug production.
Speakers
RD

Rory Dunn

Undergraduate Research Assistant, Florida Gulf Coast University
Co-authors
AS

Ankit Singh

University of Maine
NA
GP

Galen Papkov

Florida Gulf Coast University
NA
JG

John Griffis

Florida Gulf Coast University
NA
PC

Paige Carroll

Florida Gulf Coast University
NA
SB

Sarah Bauer

Mercer University
NA
ST

Seneshaw Tsegaye

Florida Gulf Coast University
NA
XY

Xiusheng Yang

University of Connecticut
NA
Wednesday September 25, 2024 12:00pm - 12:15pm HST
Lehua Suite

12:00pm HST

Come work with Driscoll’s Global R&D Team
Wednesday September 25, 2024 12:00pm - 12:30pm HST
Come to the Hort Theater to hear about exciting job opportunities with Driscoll’s, the global leader in fresh market berries! You’ll hear an overview of our business operations and locations as well as the types of roles we hire for and what we look for in prospective candidates. You may even hear a few tips and tricks to help get your application noticed that you can use with us – or other prospective employers. Please join for an informal presentation and short Q&A session.
Speakers
avatar for Logan McCollum

Logan McCollum

Senior Recruiter, Driscoll's
I work on Driscoll's talent acquisition team and enjoy talking about career choices and Driscoll's business.  I've been recruiting scientists and various business functions for Driscoll's global operations for almost 9 years.  Come by and talk about various was you can join Driscoll's... Read More →
Wednesday September 25, 2024 12:00pm - 12:30pm HST
Coral 5 - ASHS Hort Theater

12:00pm HST

Technology Applications in Horticulture (TECH) Interest Group Meeting
Wednesday September 25, 2024 12:00pm - 12:30pm HST
Moderator
Wednesday September 25, 2024 12:00pm - 12:30pm HST
Sea Pearl 3

12:00pm HST

Ornamentals Collaboration Session
Wednesday September 25, 2024 12:00pm - 1:00pm HST
A forum for discussion of potential collaborations with regards to ornamentals – i.e. floriculture, nursery crops, breeding, turf, ornamentals industry, botanic gardens, landscape industry, orchids, etc.
Moderator
MB

Megan Bowman

Ball Horticultural Company
Wednesday September 25, 2024 12:00pm - 1:00pm HST
Coral 4 - ASHS Collaboration Center

12:00pm HST

International Division Luncheon
Wednesday September 25, 2024 12:00pm - 2:00pm HST
Advance ticket purchase required.

Speaker: Dr. Wagner Vendrame, Interim Chair & Professor, Ornamental Horticulture and Biotechnology. University of Florida
International Strategies in Horticulture
Internationalization of the curriculum and research programs in horticulture has become increasingly important to us in higher education. For students, exposing them to different cultures brings a better understanding of horticultural markets and activities, unique cultural experiences, and a broader view of global issues related to agriculture. For researchers, adding an international component to your program is essential for promotion, while also bringing opportunities for collaboration and increased funding. In this presentation, I will share my international experience and discuss different strategies for internationalization of research and academic programs.
 
Dr. Vendrame leads a broad research program focusing on production and conservation of ornamental plants using plant tissue culture and cryopreservation techniques, including orchids, ornamental trees, foliage plants, palms, and other ornamental plants. In 2007, he partnered with NASA on an innovative research project to evaluate the growth, development and differential gene expression in plant cells of jatropha (Jatropha curcas), a biofuel species, as affected by microgravity, with five spaceflight studies on board of the space shuttles Atlantis, Endeavour and Discovery, and the International Space Station National Laboratory. More recently, studies have focused on cryopreservation of orchid seeds, protocorms and pollen, palm seeds, and banana in vitro shoots; and large scale propagation of plants using temporary immersion bioreactor technology, including micropropagation of orchids, bromeliads, sugarcane, banana, papaya, palms, and caçari (a tree native to the Amazon rich in vitamin C).

Moderator
LL

Leonardo Lombardini

ASHS International Division VP
Speakers
avatar for Wagner Vendrame

Wagner Vendrame

Professor & Interim Department Chair, University of Florida
Wednesday September 25, 2024 12:00pm - 2:00pm HST
Hibiscus

12:05pm HST

FRBR - Multivariate Analysis of Fruit Composition Diversity Among the North Carolina Strawberry Germplasm Collection
Wednesday September 25, 2024 12:05pm - 12:15pm HST
Strawberries are a highly sought-after fruit for their flavor, color, and nutritional benefits. In this study, strawberry fruit from 268 commercial cultivars and advanced selections from the North Carolina breeding program were evaluated to determine and characterize genotype diversity, relationships, and overall trends in fruit composition. Fifteen marketable, fully red, firm fruit were harvested per genotype between April and June of 2022 from a greenhouse core collection and held at -20°C until analysis. Juice from thawed fruit was used to collect total soluble solids (SSC), total titratable acidity (TTA), and pH data. Strawberry juice was also extracted with acidified methanol (0.1ml/1.6 ml total) and supernatants used to determine monomeric anthocyanin content (TA) using the pH differential method. Agglomerative hierarchical clustering (AHC) and multivariate analysis of variance (MANOVA) sorted genotypes into four clusters, highlighting significant differences in fruit composition profiles. The majority of commercial cultivars, and 38 advanced selections, were grouped together in Cluster 1. This cluster was distinguished by lowest SSC (7.0%), TTA (0.72%), and TA (31.22 mg/100g) values. In contrast, Cluster 2 containing 41.4% of germplasm had fruit highest in TA (54.57 mg/100g). Fruit from genotypes in Cluster 3 had high pH and %SSC, and the smallest group (Cluster 4), with 13.1% of the germplasm, had low pH and was significantly greater in %TTA. Regional strawberry breeding programs were found to group together. The Florida germplasm were grouped in Cluster 1, North Carolina commercial genotypes in Cluster 2, and two advanced New Jersey genotypic lines were found in Cluster 3. California day-neutrals were found in Cluster 1, while short-day genotypes were split between the first two clusters. Additionally, the first two principal components in PCA analysis captured 64.88% of the total variance, with both pH and TTA largely contributing to PC1 (91.1%), SSC in PC2 (71.1%), and TA associated with PC3 (77.4%). Variation in pH and %TTA had a more pronounced effect on genotype profiles and were the primary driving force for germplasm separation and differentiation. This work represents the first instance where an entire germplasm collection was assessed together in a single evaluation. The determination of genotype diversity, characterization of relationships among the genotypes and the fruit composition parameters, and visualization of trends within the collection will help guide future breeding decisions for developing new strawberry cultivars with improved quality.
Speakers
avatar for Brianna Haynes

Brianna Haynes

Graduate Student, University of Florida
Co-authors
GF

Gina Fernandez

NC State University
NA
PP

Penelope Perkins

North Carolina State University
NA
Wednesday September 25, 2024 12:05pm - 12:15pm HST
Kahili

12:10pm HST

HSMP - Effects of Thermal Processing and Drying on Nutritional Quality and Consumer Preference of Amaranthus viridis L. Value-added Product
Wednesday September 25, 2024 12:10pm - 12:20pm HST
Economic opportunities have arisen to increase the production of specialty crops catering to ethnically diverse consumers. Opportunities to capture anticipated niche market growth for ethnic crops continue to grow. Amaranthus viridis L. (amaranth), a highly nutritious leafy vegetable, eaten cooked or raw, with a nutritional value similar to spinach, is widely cultivated, highly nutritious, and has medicinal properties. Studies were conducted to 1) evaluate two thermo-processing (steam and water blanching) and three drying methods (freeze, hot air, and infrared drying) for the development of amaranth value-added products and 2) analyze the effective thermo-processing and drying methods to maximize the phytonutrient contents and minerals in the vegetable. Two thermo-treatments, steam blanch (SB) and hot water (HB), and the control (C), three drying treatments, freeze-dried (FD), hot air dried (HAD), and infrared dried (ID) were used. The treatments were T1:FD(C), T2:HAD(C), T3:ID(C), T4:FD(SB), T5:HAD(SB), T6:ID(SB), T7: FD(HW), T8: HAD(HW), T9: ID(HW). Amaranth was harvested once per week and processed three times throughout the growing season (1st harvest (28 days after transplanting (DAT)), 2nd harvest (56 DAT) in the middle of the growing season, and the 3rd harvest (84 DAT) at the end of the growing season). Phytonutrients, ascorbic acid and β-carotene, rehydration capacity, and sensory evaluation were analyzed. Significant differences in phytonutrients (total phenolic content (TPC) and total flavonoid content (TFC)) were observed among treatments and harvest times, with the FD(C) treatment being significantly higher in TPC and TFC during the 1st and 2nd harvest when compared to the other treatments. Ascorbic acid was significantly higher in FD(C) and HAD(C) (419.33 mg/100g, dm and 203.3 mg/100g, dm, respectively). ID(C), ID(SB), ID(HW) and HAD(SB) were significantly lower in ascorbic acid when compared to the other treatments. Significant differences were also observed in β-carotene among treatments. For rehydration capacity, rehydration at 9 minutes showed the highest water absorbance for all treatments, with FD(C) and HAD(C) being significantly higher, while HAD(HW) and ID(HW) were significantly lower in water absorbance for the rehydration times of 3, 6 and 9 minutes. For the sensory evaluation, 59%, 73%, and 54% of respondents indicated that the rehydrated samples were just right for flavor, color, and texture, respectively; and 45% of respondents indicated that the overall quality of the rehydrated sample was good. This study provides valuable insights for the development of value-added products catering to ethnically diverse consumers.
Speakers
MR

MEGAN REID

University of Maryland Eastern Shore
Co-authors
BM

Byungrok Min

University of Maryland Eastern Shore
NA
CN

Caleb Nindo

University of Maryland Eastern Shore
NA
avatar for Corrie Cotton

Corrie Cotton

University of Maryland Eastern Shore
Wednesday September 25, 2024 12:10pm - 12:20pm HST
South Pacific 2

12:10pm HST

VCM 2 - Cover Crops For Vegetable Research Farms Need Nitrogen
Wednesday September 25, 2024 12:10pm - 12:20pm HST
On research farms on which vegetable crops are studied, there is often a need to aggressively maintain and improve soil health, while also keeping fields ready to be assigned to research projects. We tested a protocol for keeping a Northeast farm in an appropriate condition by alternating a winter cover crop of cereal rye and a summer cover crop of forage sorghum-sudangrass with no tillage. The alternating cycle was flexible for entry points when vegetable researchers finished with an experiment, as well as for exit points to make the ground ready for an experiment. Rye was sown in late September in Geneva, NY with a no-till drill and allowed to grow to anthesis in May. Sorghum-sudangrass was sown in mid-June with the no-till drill. We tested how much nitrogen fertilizer was needed to obtain a desirable amount of growth (3 tons/acre dry mater) in the sorghum- sudangrass. The trial was done in four fields using 0, 20, 40 and 60 lb N broadcast on the rye residue at planting. The highest rate is as much as our farm would agree to invest in a cover crop, but also below forage-crop recommendations. The biomass when growth stopped due to cold (Sept 18) responded strongly to nitrogen. Without nitrogen, the biomass was only 0.5 T/ac. At 60 lb/ac, the biomass ranged among fields from 2 to 4 T/ac. Nitrogen limitation was also assessed using a SPAD chlorophyll meter. The leaves in the 0, 20 and 40 lb/ac were yellow (SPAD 22-28), with the top N rate notably greener (SPAD 30-35). The fully green control had a SPAD value of 40. The cover crop rotation kept the field in a condition where is could be prepared to being a research project with about a month’s advance notice. However, to get sufficient growth of the summer cover crop, additional nitrogen is needed. This farm is on a high-fertility silt loam, but with low organic matter from repeated research trials. In that situation, at least 60 lb/ac of actual N is needed for the cover crops to meet biomass expectations.
Speakers
avatar for Thomas Bjorkman

Thomas Bjorkman

Professor Emeritus, Cornell Horticulture
How do you move from adequate cover cropping to excellent cover cropping? What fine tuning improves carbon, nitrogen, weeds, percolation, tillage goals?We all need to advocate for horticultural research and education with those who decide to spend money on our work. It is not difficult... Read More →
Wednesday September 25, 2024 12:10pm - 12:20pm HST
South Pacific 3

12:14pm HST

Local Food Systems (LOCSY)
Wednesday September 25, 2024 12:14pm - 1:45pm HST
Exploring Markets for Horticultural Food Crops in Midwestern United States - Linda Prokopy
An expanded scope of ethnic crops research and outreach in the United States: implications on immigration and urban food - Fernanda Krupek
Youth Sustainable Agriculture Urban Farming Entrepreneurship Program: An Overview and Pilot Results from Urban Farming - Kathryn Orvis
Connecting the Dots: Creating School Garden and Produce Taste Testing Programs in Kentucky - Cindy Finneseth
Garden to Pantry: An Interactive and Interdisciplinary Approach to Growing and Preserving Produce - Rebecca Catalena
Fermented Hot Pepper Sauce as a Model for New Value-Added Opportunities for Local Food Production - Matthew Behrends
The Missouri Produce Growers Video Newsletter – Digestible, Accessible and Impactful Extension Education - Justin Keay
The Center for Sustainability of Farms and Families: Impacts of The Small Scale Farm Grant on Local Foods in Kentucky - Joni Nelson
The Waimānalo Learning Center: Land-Grant Experiment Stations as a Space to Heighten Community Engagement and Advancement - Ilima Ho-Lastimosa

Moderator
KO

Kathryn Orvis

Professor, Purdue Univ
Wednesday September 25, 2024 12:14pm - 1:45pm HST
South Pacific 1

12:14pm HST

Plant Biotechnology 2 (PB 2)
Wednesday September 25, 2024 12:14pm - 2:00pm HST
New insights into the Yellow2 locus and its role in beta-carotene accumulation in carrots (Daucus carota) - Michael Paulsmeyer
Overexpression of the Coding Sequence of Ma1 Leads to Enhanced Anthocyanin Biosynthesis in Apple via MYB73. - Mengxia Zhang
Rapid Detection and Coinfection Analysis of Aspergillus flavus in Peanut SeedsRapid Detection and Coinfection Analysis of Aspergillus flavus in Peanut Seeds - Emran Ali
RNA-seq Analysis Reveals Differentially Expressed Genes in Sweetpotato cv. Beauregard under Lead Stress - Mary Ann Munda
RNASeq Analysis Reveals Key Pathways Involved in Low Nitrogen Tolerance at the Onset of Storage Root Formation in Sweetpotato cv Bayou Belle - Lisa Arce
Introducing CHiDO – a No Code Genomic Prediction Software implementation for the Characterization & Integration of Driven Omics - Diego Jarquin
Tissue Culture-Free Genome Editing in Plants Using RNA Viruses - Degao Liu
Moderator
avatar for Michael Paulsmeyer

Michael Paulsmeyer

Postdoc, USDA-ARS
Wednesday September 25, 2024 12:14pm - 2:00pm HST
South Pacific 4

12:15pm HST

FRBR - Assessment of Fruit Composition and its Influence on Flavor and Consumer Preferences in Blackberry
Wednesday September 25, 2024 12:15pm - 12:25pm HST
Breeding efforts to improve blackberry (Rubus subgenus Rubus) flavor have previously been a subjective and imprecise process. The flavor perception of blackberries is largely controlled by interactions between sugars, acids, and volatile components. The aim of this study was to determine what combinations of these biochemical compounds result in positive flavor perception by the average consumer to assist in determining breeding targets. In the first year of data collection, consumer panelists assessed pureed frozen blackberries from the University of Arkansas Fruit Breeding Program for overall liking, aroma, flavor, sweetness and sourness across 24 sensory panels. Pearson correlation coefficients were generated to determine the relationship between overall liking and significant effects. Positive correlations to overall liking were significant for flavor intensity (0.99), aroma intensity (0.50), and sweetness intensity (0.89) indicating increases in flavor, aroma, and sweetness resulted in greater liking. Sourness was found to have a negative correlation with overall liking (-0.81). The positive correlation between liking and Brix (0.54) aligns with the perceived sweetness relationship, similarly the negative relationship between liking and titratable acidity (-0.59) aligns with perceived sourness. Panelists’ perceptions of fruity or indulgent flavors such as grape, banana, strawberry, apple, jam and candy produced significant positive correlations with overall liking. While fruit presenting flavors described as citrus, grassy, earthy, musty, pine, or chemical produced significant negative correlations with overall liking. The volatiles associated with these perceived flavors were also assessed using gas chromatography mass spectrometry.
Speakers
KL

Katelyn Lust-Moore

University of Arkansas
Co-authors
JL

Jackie Lee

University of Arkansas
NA
MW

Margaret Worthington

University of Arkansas
NA
RT

Renee Threlfall

University of Arkansas
NA
SF

Samuel Fernandes

University of Arkansas
NA
SL

Scott Lafontaine

University of Arkansas
NA
Wednesday September 25, 2024 12:15pm - 12:25pm HST
Kahili

12:15pm HST

LOCSY - Exploring Markets for Horticultural Food Crops in Midwestern United States
Wednesday September 25, 2024 12:15pm - 12:25pm HST
Agricultural communities in the Midwest face ongoing economic, environmental, and social challenges. A variety of research shows that increasing diversity in an agricultural system can help overcome these challenges. The Diverse Corn Belt (DCB) is a USDA-NIFA funded project focuses on developing an evidence-based framework and vision of how to enable a more diverse agricultural landscape across the Corn Belt (Illinois, Iowa, and Indiana). This interdisciplinary project is exploring and promoting diversity at the farm, landscape, and market levels through Research, Extension, and Education. We have completed 18 focus groups across Indiana, Illinois and Iowa with conventional and diversified farmers, interviews with horticultural growers, and surveys of retailers, processors, and consumers. Last year we presented results from focus groups and interviews. This year, we add results from survey findings to highlight gaps between different stakeholders in the horticultural value chain, as well as the most valuable food labels among consumers, retailers, and processors.
Speakers
LP

Linda Prokopy

Purdue University
Co-authors
AT

Ariana Torres

Purdue University
NA
Wednesday September 25, 2024 12:15pm - 12:25pm HST
South Pacific 1

12:15pm HST

PB 2 - New insights into the Yellow2 locus and its role in beta-carotene accumulation in carrots (Daucus carota)
Wednesday September 25, 2024 12:15pm - 12:30pm HST
Carrots (Daucus carota) are a unique model for the accumulation of carotenoids. Beta-carotene accumulates in large amounts in the taproot if the proper alleles of the following three loci are present: OR, Y, and Y2. These three loci are not carotenoid biosynthetic genes but rather post-transcriptional regulation of carotenoid accumulation. The genes underlying the OR and Y loci have been characterized, but the gene underlying the Y2 locus is unknown. Through genomic and transcriptomic analyses, a single candidate that may interact with light signaling was found. To determine the function of this gene, the functional transcript from wild carrot was overexpressed in orange carrots and used in a transient infiltration assay with a GFP fusion tag in tobacco. The orange allele of this gene has a large transposon insertion that theoretically inactivates the gene. However, full length transcript can still be detected in orange carrots. This begs the question of whether the transposon is still active in certain accessions. In this study, the proportion properly assembled Y2 transcript was analyzed via qRT-PCR. A KASP marker was also developed to assist plant breeders in selection for the Y2 locus.
Speakers
avatar for Michael Paulsmeyer

Michael Paulsmeyer

Postdoc, USDA-ARS
Co-authors
PS

Philipp Simon

USDA-ARS
NA
Wednesday September 25, 2024 12:15pm - 12:30pm HST
South Pacific 4

12:15pm HST

PH 2 - Isolation of Pathogenic Strain of Trichoderma atroviride from Symptomless, Disease-Free Sweetpotato (Ipomoea batatas L.) Storage Roots.
Wednesday September 25, 2024 12:15pm - 12:30pm HST
Many fungal endophytes have the ability to promote plant growth, as well as increase the host plant’s tolerance to abiotic and biotic stresses. Most endophytic species of Trichoderma are well known biocontrol agents and have been used to control diseases caused by phytopathogens of the genera Rhizoctonia, Fusarium, and Phytophthora. However, our recent research has isolated a strain of Trichoderma from sweetpotato storage roots purchased from a local farm, which was shown to develop root rot symptoms. Therefore, the objective of this project was to evaluate this isolate, especially for its potentially pathogenic nature. In the laboratory, the ITS region of the isolate was amplified, and gene sequencing placed it to T. atroviride with 99% homology. However, T. atroviride has never been identified as a disease-causing agent for sweetpotato in literature so far. Pathogenicity test was carried out accordingly, by inoculating this isolate onto healthy, symptomless sweetpotato storage roots. Two weeks post-inoculation, the storage roots were cut in half to reveal necrotic lesion development between 1.7 and 2.1cm in diameter based on three replications. The necrotic tissue was sampled and cultured on PDA, and the re-isolated fungal specimen was confirmed to be T. atroviride through gene sequencing. This result demonstrated that under environmental conditions conducive for pathogen development, this strain of T. atroviride is capable of causing root rot disease in sweetpotatoes. This finding is particularly significant as farmers typically store sweetpotatoes for an extended time, sometimes up to a year, which increases the possibilities for disease occurrence. Dual-culture assays are still ongoing to determine if this strain of T. atroviride could be a beneficial endophyte under different storage conditions, especially when other fungal endophytes are co-existing in the same storage root.
Speakers
RN

Richard Noel Torres

University of Missouri - Columbia
Co-authors
JS

James Schoelz

University of Missouri - Columbia
NA
PT

Peng Tian

University of Missouri - Columbia
NA
XX

Xi Xiong

University of Missouri - Columbia
NA
Wednesday September 25, 2024 12:15pm - 12:30pm HST
Nautilus

12:15pm HST

UG - From Flower to Fruit: Growing Degree Days and Peach Ripening
Wednesday September 25, 2024 12:15pm - 12:30pm HST
Anticipating crop advancement, particularly fruit maturation, is critical for peach growers' success and marketing. Growing Degree Days (GDD) predict the growth and development stages of plants and insects. They are based on the accumulation of heat units above a specific baseline temperature, under the concept that a certain amount of heat is needed to develop from one stage to another in the life cycle. GDDs are used for various purposes in agriculture and horticulture, such as planting scheduling, pest management and crop monitoring. Peach growers use GDD to predict the peach cultivar maturity and schedule harvesting. However, peach cultivars' ripening time is reported in the calendar or Julian days (JD) or as the number of days before or after a reference cultivar, which is not amenable to climate change. Therefore, we modeled GDD in a diverse set of peach and nectarine cultivars and breeding accessions using the Baskerville-Emin (BE) method. The GDD was calculated from full bloom to fruit maturity using historical temperature, bloom and ripening data collected at the Musser Fruit Research Station in Seneca, South Carolina, in the 2017-2023 period. GDD and JD variability will be presented, and implication of providing GDD information on existing and newly released cultivars for producers and researchers will be discussed.
Speakers
MA

Matthew Almy

Clemson University
NA
Co-authors
JM

John Mark Lawton

Clemson University
NA
KG

Ksenija Gasic

Clemson University
Wednesday September 25, 2024 12:15pm - 12:30pm HST
Lehua Suite

12:19pm HST

Growth Chambers and Controlled Environments - (CE)
Wednesday September 25, 2024 12:19pm - 1:30pm HST
Urban Agriculture Efficiency: A Year-Long Evaluation of Kale Yield and Energy Use in a Shipping Container Farm - Skyler Brazel
Production of Red Leaf Lettuce at Vertical Farm Equipped with Blue LED According to Anthocyanin Concentration and Its Gene Analysis - Junjira Satitmunnaithum
Interlighting Improves Tomato Yield in Northern Greenhouses - Meriam Karlsson
Effect of low root-zone temperature and UV radiation on growth and gene expression of secondary metabolite pathways in Nicotiana benthamiana - KiHo Son
Effects of Short-Wavelength UV-B, UV-A, and Blue Light on the Rose Flower Color Transition Phenotype - Navdeep Kaur
Arduino Uno Can Reliably Log Substrate Moisture from a Bus of Digital Sensors and Control a Drip-Irrigation System - Stephanie E Burnett
The Effect of Climate Change on Plants: A Case Study with Wheat - Kira Webster

Moderator
SB

Skyler Brazel

University of Tennessee - Knoxville
Wednesday September 25, 2024 12:19pm - 1:30pm HST
Coral 1

12:20pm HST

CE - Urban Agriculture Efficiency: A Year-Long Evaluation of Kale Yield and Energy Use in a Shipping Container Farm
Wednesday September 25, 2024 12:20pm - 12:30pm HST
Urban expansion is a threat to agricultural land. As cities increase in size and residential areas are being built on arable land, a new solution to growing food in urban area needs to be considered. Shipping container farms are designed to grow a high number of plants in a small area. These farms are programmable to fit the environmental parameters that are optimal for each type of crop. In this experiment, ‘Toscano’ kale was grown inside the farm and harvested weekly for one year to simulate farm production. The average day/night temperature in the farm was 22.8C and 15.6C with a photoperiod of 20 hours. Sole sourced lighting was supplied by light emitting diodes with an 80:20 red:blue ratio and an intensity of 100 mol . m -2. s -1 for a daily light integral of 7.2 mol . d -1 . Electrical energy use of the farm was collected on three categories of energy usage: Lighting, HVAC, and all Other Energy usage. Seeds were sown four weeks prior to transplant. Plants were transplanted weekly into vertical channels and harvested 12 weeks after sowing. This experiment was a complete block design with block nested in time. Yield data was collected at time of harvest, including plant number, fresh mass, dry mass, plant height, canopy area, and leaf number. The mean number of plants per replication was 320. The mean fresh mass per plant by block was 43.34g, 48.84g, 53.17g, 59.15g, 57.88g, and 53.29g, respectively, while mean dry mass was 3.31g, 3.66g, 3.94g, 4.42g, 4.3g, and 4.08g, respectively. Daily mean lighting and other energy consumption exhibited no variation across all 48 harvests. Daily mean HVAC energy consumption varied based on outdoor environmental conditions, with increased usage during summer months and a maximum of 33.53 kWh/day. Overall, fluctuations across mean fresh mass needs to be investigated further as the optimal harvest date for this farm may occur prior to 12 weeks, for both plant yield and energy consumption levels.
Speakers
SB

Skyler Brazel

University of Tennessee - Knoxville
Co-authors
CS

Carl Sams

University of Tennessee
KR

Katelynn Rector

University of Tennessee - Knoxville
NA
Wednesday September 25, 2024 12:20pm - 12:30pm HST
Coral 1

12:20pm HST

HSMP - Ginseng Growth and Ginsenoside Contents According to Light Intensity
Wednesday September 25, 2024 12:20pm - 12:30pm HST
Ginseng (Panax ginseng C.A. Meyer) is mainly cultivated in the Republic of Korea and China, and has been traditionally used as a medicinal plant in East Asia for the treatment of diseases such as hypertension, diabetes mellitus, liver and kidney dysfunction, mental disorders, and skin inflammation. Ginseng roots are cultivated after maturing at 3 to 6 years of age. The harvested ginseng roots are consumed and sold in various forms of products, such as fresh ginseng, red ginseng, and white ginseng. Ginseng is a shade-tolerant crop and requires shading facilities. It grows well with a light intensity of 10%, but its growth is hindered at light intensity above 20% due to high temperature. Therefore, it is cultivated with a light intensity set to 10% from April to October and harvested in October. However, if early harvesting is done in August to increase farm income, growth is low due to insufficient light. Therefore, a cultivation experiment was conducted with the light intensity set to 10% and 20% for the August harvest, and the yield and ginsenoside contents were compared with the October harvest. The yield of the 20% light intensity treatment group was 665 kg/10a, whereas the yield of the 10% light intensity treatment group was 572 kg/10a. The yield of the 20% treatment group was 16.3% higher than that of the 10% treatment group. According to the analysis on nine ginsenoside components including Rg1, the total ginsenoside contents in the 20% light intensity treatment group was 27.51 mg/g, which was 75.3% higher than that of the 10% light intensity treatment group at 15.69 mg/g. Specifically, Rg1, Re, Rb1, and Rc were 3.40, 7.29, 7.93, and 3.84 mg/g, respectively, more than twice as high as those in the 10% light intensity treatment group. Conclusively, a light intensity of 20% was determined to be optimal for maximizing ginsenoside cultivation for August harvesting.
Speakers
MK

Minhee Kim

South Korea, Ginseng&Medicinal Plant Research Institute, Chungcheongnam-do Agricultural Research and Extension Services
Co-authors
KS

Ka Soon Lee

Ginseng
NA
SI

Sun Ick Kim

Ginseng
NA
Wednesday September 25, 2024 12:20pm - 12:30pm HST
South Pacific 2

12:20pm HST

VCM 2 - Insurance Industry Standards Over-estimate Yield Loss Due to Stand Reduction in Processing Sweet Corn
Wednesday September 25, 2024 12:20pm - 12:30pm HST
Sweet corn is an important processing crop in the upper Midwestern United States. It can be insured for hail losses, but actuarial tables are based on field corn. The first step in developing accurate yield loss assessments is determining yield loss due to complete plant loss. To that end, we compared ear yield of sweet corn thinned to 75, 50, or 25% of a full population at three times throughout the growing season to control plots that were not thinned. We repeated this in three consecutive years at two locations, without irrigation or supplemental fertilizer. Average yield for control plots was 16.4–20.3 Mg•ha-1. Average yield losses due to thinning were generally less than currently-used actuarial estimates. For example, average yield of sweet corn when thinned to 50% of a full population at growth stage V3–V5 was 88–96% (95%CI) of full yield. The actuarial estimate is 78% of full yield, indicating greater resilience than expected. The number of tillers per plant was greater in all thinned plots, especially those thinned early in the season. Kernel recovery was unaffected by thinning treatment in one location and declined slightly at heavy thinning in the other location. Sweet corn actuarial tables should be different from actuarial tables based on field corn.
Speakers
avatar for Charlie Rohwer

Charlie Rohwer

Scientist, University of Minnesota
Co-authors
JL

Joe Lauer

University of Wisconsin-Madison
NA
MZ

Mark Zarnstorff

National Crop Insurance Services
NA
Wednesday September 25, 2024 12:20pm - 12:30pm HST
South Pacific 3

12:25pm HST

FRBR - Improving Blueberry By Dissecting Fruit Texture
Wednesday September 25, 2024 12:25pm - 12:35pm HST
Blueberry texture is a critical trait impacting mechanical harvesting, transportation resilience, shelf life, and consumer preference. Its complexity arises from multiple attributes, requiring a depth understanding for developing breeding tools to generate varieties with superior texture profiles. In this study, a diverse group of southern highbush blueberry (SHB) genotypes, reflecting various textures, were subjected to mechanical phenotyping, consumer perception analysis, and cell wall composition analysis. By conducting instrumental analysis using a texture analyzer, three key parameters crucial for genotype differentiation based on texture were identified. Consumer perception analysis revealed moderate agreement in texture classification between breeders and panelists. Additionally, cell wall composition analysis highlighted galacturonic acid as a potential monosaccharide capable of categorizing texture. The integration of mechanical phenotyping, consumer perception analysis, and cell wall composition analysis offers a comprehensive approach to dissecting blueberry texture. This enables breeders to select and develop blueberry varieties meeting consumer expectations, enhancing market competitiveness, and supporting the growth and sustainability of the global blueberry industry.
Speakers
avatar for Juan David Jimenez Pardo

Juan David Jimenez Pardo

PhD student, University of Florida
Wednesday September 25, 2024 12:25pm - 12:35pm HST
Kahili

12:25pm HST

LOCSY - An expanded scope of ethnic crops research and outreach in the United States: implications on immigration and urban food
Wednesday September 25, 2024 12:25pm - 12:35pm HST
The participation of immigrant, refugee and communities of conflict-displaced former agricultural professionals in urban agriculture and local food systems has steadily increased in the United States over the past decades. Even though production and access of culturally appropriate crops has the potential to nourish the health and social fabric of communities and contribute to the local economy, less is known about the extant literature related to research and outreach on ethnic crops. This presentation will cover preliminary results of an ongoing systematic review collating peer-reviewed and gray literature published from 2000 to the present to identify, map, and describe existing research and outreach efforts on culturally relevant crops in the United States. Our findings will have the potential to offer a greater understanding of the current state of knowledge to inform the focus of future research on innovative models for more sustainable and local urban production.
Speakers
FK

Fernanda Krupek

Ohio State University
Co-authors
JK

Jacqueline Kowalski

University of Connecticut
NA
Wednesday September 25, 2024 12:25pm - 12:35pm HST
South Pacific 1

12:29pm HST

Pomology 2/Horticultural Plant Reproductive Biology (POM 2/HPRB)
Wednesday September 25, 2024 12:29pm - 1:50pm HST
Mechanization Reduces Pruning and Harvest Labor Time in the Cider Apple Orchard - Carol Miles
Testing Agronomic Strategies to Reduce Biennial Bearing in Apples - Thiago Campbell
Identifying Local Apple Germplasm for Use of Hard Ciders in North Dakota - Wenhao Dai
The Fruitlet Size Distribution Model As A Thinning Decision Aid For Precision Crop Load Management Of Apple - Laura Hillmann
Deciphering the Proline-Mediated Flowering Delay Mechanism in Peach through Physiological and Metabolic Analyses - Hyunsuk Shin
Genetic Architecture of Important Traits for Prunus Crop Improvement - Michael Itam
Taste Acidity and Other Important Fruit Characteristics of New Peach Varieties Compared with Concurrent Varieties During 2022 and 2023 - Hemant Gohil
Role of Silicon in Peach Water Stress Tolerance - Noah Willsea
Moderator
TC

Thiago Campbell

Washington State University
Wednesday September 25, 2024 12:29pm - 1:50pm HST
Coral 2

12:30pm HST

CE - Production of Red Leaf Lettuce at Vertical Farm Equipped with Blue LED According to Anthocyanin Concentration and Its Ge
Wednesday September 25, 2024 12:30pm - 12:40pm HST
At the last ASHS annual conference, I, Satitmunnaithum et al., (2023), presented our study on the effect of blue and white LED light ratio on red leaf lettuce, however, the effect of blue LED on red coloration during its growth is still unclear. Thus, at this year’s conference, we aim to clarify the mechanisms of red coloration under blue LED based on anthocyanin biosynthesis gene analysis and its content in vertical farming condition to stabilize its production for high market demand. To elucidate the effect of blue LED light on the red coloration of red leaf lettuce, green and red leaf lettuce (Lactuca sativa L.) were selected for this study. Both were hydroponically cultivated at the Advanced Plant Factory Research Center at Meiji University, Japan. The cultivation temperature was set at 22 ℃ with a humidity of 60%. Seeds were sown under white LED for 24 hours. Ten-day-old seedlings were transplanted to different light conditions: white LED and blue LED. The photoperiod was set for 16 hours. The nutrient solution was supplied at an EC of 1.6 mS/m^2 with a pH of 6.0 ± 0.5. Both light treatments had a PPFD of 100-120 µmol/m^2/s. After 20, 25, and 30 days of transplantation, lettuces were harvested. The red area on leaf lettuce, along with the total anthocyanin content and its precursors, as well as the expressions of anthocyanin biosynthesis-related genes such as ANS, CHS, bHLH, DFR, and HY5, were evaluated. Blue light shows a large red area on red leaf lettuce at most of the development stages resulting in a high red area percentage, while green leaf lettuce remained completely green in both light conditions. The interested genes were upregulated mostly in blue light irradiated red leaf lettuce which led to high total anthocyanin content. This can be assumed that blue LED light enhances anthocyanin synthesis in red leaf lettuce which can contribute to the stable production of red leaf lettuce in vertical farms.
Speakers
JS

Junjira Satitmunnaithum

Specially-appointed lecturer, Organization for the Strategic Coordination of Research and Intellectual Properties, Meiji University , Japan
Co-authors
CI

Chiharu Inoue

Daiwa House Co. Ltd.,
NA
TO

Takao Otsuka

Daiwa House Co. Ltd.,
NA
TI

Takashi Ikeda

School of Agriculture, Meiji university
NA
Wednesday September 25, 2024 12:30pm - 12:40pm HST
Coral 1

12:30pm HST

HSMP - Evaluation of anticancer activity of perennial tree seed-derived extracts on various cancer cell lines
Wednesday September 25, 2024 12:30pm - 12:40pm HST
According to the WHO, the global cancer incidence rate is increasing by more than 5% annually due to the rapid increase in the elderly population and continuous environmental degradation. The increasing rate of cancer incidence underscores the importance of developing health supplements and pharmaceutical ingredients derived from plants. Indeed, several anticancer drugs such as Taxol, vincristine, and vinblastine have been developed from plant-derived ingredients, serving as crucial resources in modern pharmaceutical development. This study evaluated the anticancer activity of extracts from seven different seeds against various cancer cell lines. Seeds from perennial trees such as Alnus japonica, Chamaecyparis obtusa, Cornus kousa, Phellodendron amurense, Pinus densiflora, Prunus sargentii, and Quercus glauca were used in the experiments. Extracts, prepared via ultrasonic extraction with 70% ethanol and concentrated to 100 µg·mL-1, were tested on lung (A549), prostate (LNCaP-LN3), melanoma (B16F10), colon (Caco-2, HCT15), and breast (MDA-MB-231) cancer cell lines using the MTT assay. In the lung cancer (A549) cell line, C. kousa, C. obtusa, and Q. glauca significantly inhibited cancer cell proliferation compared to the negative control (DMSO), with viability rates of 68.2%, 6.8%, and 44.7%, respectively. Prostate cancer (LNCaP-LN3) cells showed anticancer activity with extracts from six species, excluding P. sargentii, in the following order of cell viability: C. obtusa (8.2%) > C. kousa (15.8%) > A. japonica (35.1%) > Q. glauca (73.9%) > P. amurense (78.6%) > P. densiflora (86.3%). Cell viability was assessed for colon cancer cell lines Caco-2 and HCT15, where extracts from C. kousa (71.7% for Caco-2 and 69.9% for HCT15), C. obtusa (8.0% for Caco-2 and 7.1% for HCT15), and Q. glauca (89.9% for Caco-2) demonstrated significant inhibition of cell proliferation. For melanoma (B16F10) and breast cancer (MDA-MB-231) cell lines, extracts from C. kousa (63.8% and 66.3%, respectively) and C. obtusa (6.7% and 7.3%, respectively) showed strong anticancer activity, significantly reducing cell viability. This study suggests that the extracts from seven species of seeds can have a potent anticancer effect on specific cancer cell lines and confirms their potential as an important step in the development of anticancer ingredients derived from perennial tree seeds.
Speakers
HL

Hamin Lee

Chungbuk National University
Co-authors
JC

Ju-Sung Cho

Chungbuk Nationl University
NA
KP

Kyungtae Park

Chungbuk Nationl University
NA
YK

Youg-Rak Kwon

National Forest Seed Variety Center
NA
Wednesday September 25, 2024 12:30pm - 12:40pm HST
South Pacific 2

12:30pm HST

POM 2 - Testing Agronomic Strategies to Reduce Biennial Bearing in Apples
Wednesday September 25, 2024 12:30pm - 12:40pm HST
Biennial bearing in tree fruit can be problematic, and these patterns are not unique to tree fruit and occur in natural species. Masting, or the abundant production of seeds in some years, can also be observed in natural tree species. Despite extensive research, biennial bearing still affects commercial orchard production. Biennial bearing cycles are costly, with inconsistent yields and fruit quality occurring yearly. Crop load management is one commonly used management strategy to control biennial beating. However, trees can continue a biennial cycle the year following adequate crop thinning. We evaluated six different agronomic strategies to combat biennial bearing in apple trees; two for low-cropping trees (“off” year) and four for high-cropping trees (“on” year). In 2023, ‘Honeycrisp’ trees on B.9, G.41, G.890, and M.9-T337 rootstocks in their high-cropping year were root pruned, girdled, sprayed with ethephon, or fertilized with nitrogen (ammonium nitrate) in the fall, along with untreated control. Trees in their low-cropping year (2023) were defoliated and sprayed with gibberellic acid (GA 3 ) and untreated control. Due to the limited availability of low-cropping trees in 2023, only ‘Honeycrisp’ trees on B.9 and M.9-T337 rootstocks were used. All treatments were applied two to three weeks after full bloom except for nitrogen fertilization, which was applied in late August. Crop load, vegetative growth, and fruit quality were measured for treated trees and controls. Carryover effects of treatments were calculated to quantify the reduction/amelioration of biennial bearing incidence. In low-cropped trees, GA 3 had significantly higher return bloom from controls and defoliated trees. In high-cropped trees, no treatment had a significant effect on return bloom compared to controls. Vegetative growth was not affected by any treatment. Effective agronomic strategies for managing biennial bearing can be inconsistent and need to be further investigated for their impacts.
Speakers
TC

Thiago Campbell

Washington State University
Co-authors
LK

Lee Kalcsits

Washington State University
NA
Wednesday September 25, 2024 12:30pm - 12:40pm HST
Coral 2

12:30pm HST

VCM 2 - Combining Lasers and Distress Calls to Control Birds in Sweet Corn
Wednesday September 25, 2024 12:30pm - 12:40pm HST
Red-winged blackbirds (Agelaius phoeniceus) and European starlings (Sturnus vulgaris) cause significant damage to sweet corn (Zea mays). Even minor damage can render ears unmarketable and the need to sort out damaged ears during packing increases costs for growers. Propane cannons and pyrotechnics have long been the preferred methods for preventing birds from damaging corn, but both methods create noise pollution and conflicts with farm neighbors. Automated laser scarecrows and automated recordings of bird distress and predator calls are two newer technologies for deterring birds from crops. We tested both technologies on a research farm in Kingston, Rhode Island and in commercial processing sweet corn fields in western New York in 2022 and 2023. All experiments used naturally occurring flocks of wild birds; flock size and species makeup varied between fields and over time. The automated laser scarecrows (LS) were designed and built by the URI Laser Scarecrow Project; they utilized a 50 mW 532 nm green laser with a beam diameter of 14 mm at the aperture and a dispersion of 4 mrad. Vertical and horizontal movement of the beam was randomized by a microcontroller. Bird Gard Super Pro units (BG) randomly played digital recordings of distress and alarm calls of red-winged blackbirds and European starlings and hunting calls of hawks and falcons. Data were collected as counts of damaged ears and converted to percentage of total ears for analysis. In Rhode Island the effect of laser scarecrow alone was compared to the effect of the laser scarecrow combined with the bird distress calls. In New York the two deterrent treatments were also compared to an unprotected control. In Rhode Island in 2022 bird damage averaged 20.7% with just LS and 7.1% with LS BG; the difference was significant at P
Speakers Co-authors
JK

Julie Kikkert

Cornell Cooperative Extension
NA
Wednesday September 25, 2024 12:30pm - 12:40pm HST
South Pacific 3

12:30pm HST

PB 2 - Overexpression of the Coding Sequence of Ma1 Leads to Enhanced Anthocyanin Biosynthesis in Apple via MYB73.
Wednesday September 25, 2024 12:30pm - 12:45pm HST
Anthocyanins, a group of secondary metabolites synthesized in the phenylpropanoid pathway, largely determine fruit peel color of fleshy fruits, but it is not known if its synthesis is linked to vacuolar malate accumulation that determines fruit acidity. Here, we show that when the coding sequence of Ma1, the major gene controlling apple fruit acidity, is overexpressed in ‘Royal Gala’ (RG), anthocyanin biosynthesis in the fruit peel is enhanced, corresponding to the downregulation of the expression of MYB73, an R2R3-MYB transcription factor. RNAi suppression of MYB73 expression via virus-induced gene silencing increases anthocyanin biosynthesis whereas its transient overexpression decreases anthocyanin biosynthesis in apple fruit peel. MYB73 binds to the promoter of the gene encoding UDP-glycose: flavonoid-3-O-glycosyltransferase (UFGT), the enzyme that catalyzes the last step in anthocyanin synthesis, to repress its expression. When MYB73 expression is suppressed by RNAi, UFGT expression is enhanced, leading to more anthocyanin synthesis, but this effect is blocked by RNAi suppression of UFGT expression. RNAi suppression of MYB73 enhances anthocyanin synthesis in wild-type RG apples whereas its overexpression decreases anthocyanin synthesis in Ma1-OE fruit. In the meantime, MYB73 competes with MYB1, one of the key activators of anthocyanin biosynthesis, binding to the promoter of UFGT and regulating its expression. These results indicate that MYB73 negatively regulates anthocyanin biosynthesis via repressing UFGT expression in apple peel. In Ma1-OE fruit, down-regulation of MYB73 releases UFGT from MYB73 repression and allows more MYB1 binding to UFGT promoter, leading to enhanced anthocyanin biosynthesis.
Speakers
MZ

Mengxia Zhang

Cornell University
Co-authors
DH

Dagang Hu

Cornell University
NA
LC

Lailiang Cheng

Cornell University
NW

Nan Wang

Cornell University
NA
Wednesday September 25, 2024 12:30pm - 12:45pm HST
South Pacific 4

12:30pm HST

UG - Enhancing rose propagation using moisture sensor-controlled irrigation and LED supplemental lighting in greenhouses
Wednesday September 25, 2024 12:30pm - 12:45pm HST
This study addresses the critical need for precise irrigation management in the greenhouse production of high-value ornamental crops, focusing on the propagation of single-stem rose (Rosa rubiginosa) cuttings under light-emitting diode (LED) supplemental lighting. The current lack of effective monitoring and control systems for substrate moisture poses challenges in optimizing plant growth while minimizing water and nutrient losses. In this context, we propose the integration of moisture sensors for real-time monitoring and control of substrate moisture levels, coupled with LED supplemental lighting, to enhance the production of rose cuttings. Our approach involved assessing the feasibility and effectiveness of moisture sensor-controlled irrigation in greenhouses, considering the specific requirements of rose propagation and the influence of LED lighting on plant growth. We tested three Ө thresholds (0.25, 0.35, and 0.45 m3.m-3) and five light levels as supplemental lighting (100, 175, 250, 325, and 400 µmol.m-2.s-1) arranged on randomized complete block design with four replications. Rose Double Knock Out® ‘Radtko’ PP 16,202 CPBR 3,104 plants were grown in 15.6 L pots (Pioneer Pots; Blackmore Co., Belleville, MI) filled with 20% Canadian peat/58% aged pine/10% perlite/12% EZ Hydrafiber lime potting mix (Oldcastle HFC25; Oldcastle Lawn
Speakers
BT

Braylen Thomson

Undergrad Researcher, University of Georgia
Co-authors
AH

Alan Huber

University of Georgia
NA
BH

Brandon Heavern

James Greenhouses
NA
KJ

Ken James

James Greenhouses
NA
KQ

Kuan Qin

University of Georgia
LJ

Lars Jensen

Blackmore Company
NA
MH

Matthew Housley

University of Georgia
RF

Rhuanito Ferrarezi

University of Georgia
NA
Wednesday September 25, 2024 12:30pm - 12:45pm HST
Lehua Suite

12:30pm HST

ASHS Plants, People, Science Podcast Live Episode
Wednesday September 25, 2024 12:30pm - 1:30pm HST
ASHS Podcast - People, Plants, Science - LIVE Episode – The Science and Conservation of Breadfruit with expert Heidi Leianuenue Bornhorst, O’ahu Coordinator for the Breadfruit Institute.
Be a part of our conversation as we take a deep dive into breadfruit with our guest, Heidi Leianuenue Bornhorst. At this live podcast episode, we will talk with Heidi about the history of the breadfruit, its long-standing significance as an important fruit tree in tropical regions, and what work is being done to conserve existing trees and increase future growth.
Heidi Leianuenue Bornhorst is a Horticulturist and Certified Arborist specializing in native Hawaiian plants, Horticulture, Xeriscaping and Restoration of native habitats.
Heidi has an M.S. in Horticulture and a Certificate in Public Administration. She is a speaker and garden writer.
She has worked in Leadership positions in public gardens, including the Hale Koa Hotel, Honolulu Botanical Gardens, (Foster, Wahiawa, Liliuokalani, Koko Crater, and Ho`omaluhia), and as Horticulturist at the Honolulu Zoo. She has also worked at the National Tropical Botanical Garden on Kaua`i, Lyon Arboretum, U.H. Manoa and Kupuna for Hawaiian Studies, DOE.
She is a landscape design and maintenance Consultant, with her company Pruning for Productivity, helping people grow their Dream Gardens (and be practical and scientific too), and as an Arborist, providing right sizing and professional pruning of valuable fruit trees.
She is the O`ahu Coordinator for the Breadfruit Institute helping with `Ulu tree distribution, Horticulture, proper tree trimming, and long-term care and maintenance.
Heidi was a founding partner of the Mangoes at the Moana Hotel annual festival, where she spoke about caring for and proper pruning and planting of mango trees, a judge in the Best Mango tasting contest and Mango recipe contest. Our goal: A Fruit tree in every Hawaii Garden!
She got her start as a Volunteer at Foster Garden, and as an Apprentice Gardener at the National Tropical Botanical Garden. She holds a B.S. and M.S. from U.H. Manoa. She was an apprentice gardener at Longwood Gardens in Pennsylvania, and at the RHS Wisley Botanic Garden in England.
She authored the book: Growing Native Hawaiian Plants and wrote the Hawai`i Gardens column for the Honolulu Star Advertise for 30 years.
She was the Garden expert on ‘the Morning Garden’ early morning KITV.
She is a noted public speaker, expert tour guide, and Hike leader.
Speakers
avatar for Curt Rom

Curt Rom

University Professor. And Associate Director, University of Arkansas and ASHS
SH

Samson Humphrey

Univ. of Tennessee
N/A
Wednesday September 25, 2024 12:30pm - 1:30pm HST
Sea Pearl 4

12:35pm HST

FRBR - Sugar Content and Gene Expression Dynamics in Apple Spurs in Response to Fruit Load and Gibberellins
Wednesday September 25, 2024 12:35pm - 12:45pm HST
Many commercial apple (Malus x domestica Borkh.) cultivars follow a biennial bearing cycle, alternating between an “on” year with a heavy crop load and an "off" year with few if any fruit. It is commonly thought that biennial bearing is conditioned by repression of floral initiation by developing fruit. Although various studies have provided some insights into the regulation of flowering and biennial bearing by fruit load, the associated molecular-genetic pathway involved remains unclear. The objective of this study is to evaluate the influence of fruit load on the expression of specific, presumed flowering genes in the spur leaves and shoot apex, focusing on the potential role of sugars in this process. The hypothesis is that the potential accumulation of photosynthates in the leaf or shoot apex related to the absence of a strong sink (developing fruit) promotes floral initiation by directing the expression of floral-promotive genes. To test this, physiologically similar ‘Royal Red Honeycrisp’ trees were manipulated to influence fruit load. The accumulation of soluble sugars (fructose, sorbitol, glucose, and fructose) in the spur leaf and shoot apex was evaluated using a gas chromatograph. RNAs were extracted and gene expression patterns in the leaf and apex were evaluated using quantitative RT-PCR and direct sequencing; results will be presented. This study should provide new insights into the fundamental mechanisms of biennial bearing in apple. This may lead to approaches to limit the impact of biennial bearing in existing orchards, and genetic information useful for apple cultivar improvement.
Speakers
QF

Qiuyi Fu

Michigan State University
Co-authors
SV

Steve van Nocker

Michigan State University
NA
Wednesday September 25, 2024 12:35pm - 12:45pm HST
Kahili

12:35pm HST

LOCSY - Youth Sustainable Agriculture Urban Farming Entrepreneurship Program: An Overview and Pilot Results from Urban Farming
Wednesday September 25, 2024 12:35pm - 12:45pm HST
Urban agriculture initiatives developed for youth have the capacity to motivate learning, include minority groups, and allow participation in activities that foster self-growth, community engagement, and entrepreneurship. This pilot study builds on a partnership between a midwestern university and the Felege Hiywot Center’s (FHC) successful youth urban farming program, now focusing on how that programing could contribute to developing life skills, sustainable agriculture knowledge, and entrepreneurial skills. A mixed methods approach was utilized, including pre/post surveys, student presentations, and observations. Participants were twenty-eight high school aged youth, of which 91% self-identified as African American. Main foci of the project were participants’ life skills, entrepreneurial skills and mindset, sustainable agriculture content, and community collaborations. Key findings from the summer pilot post survey (n=23) indicate that 70% of participants responded that they could develop their own business plan, 83% believe that external changes create opportunities for an urban ag business; and 65% believed they could build connections with people they had not before. Knowledge around sustainable agriculture shows room for improvement where only 30% could identify what percentage of food grown globally is wasted annually, and 43% believed that technology development, adoption and utilization is not a part of sustainable agriculture; however, sixty five percent of participants could identify the three sustainability pillars: economics, environment, and social. The program's influence on participants' future plans was of interest, with 52% self-reporting a change in their outlook. This impact can be attributed to the collaborative efforts of volunteers, professionals, sponsors, and the structured nature of the FHC program, which also emphasizes the importance of individual backgrounds. The potential to impact and enhance life skills, foster self-awareness, improving family communication, and open avenues for future education and career opportunities will allow for a broader development and expansion of this project. This pilot contributes to the broader discourse on youth programs in urban agriculture by highlighting the integrative approach of experiential learning, farm-based education, and student-centered learning within a youth development framework. While early in the project, this pilot shows promise in underlining the critical role of urban agriculture programs in shaping the lives and future prospects of minority youth.
Speakers
KO

Kathryn Orvis

Professor, Purdue Univ
Co-authors
AB

Aster Bekele

Felege Hiywot Center
NA
DI

Delphine Iradukunda

Purdue Univ
NA
NS

Nathan Shoaf

Purdue University
NA
TN

THEONESTE NZARAMYIMANA

Kentucky State University
Wednesday September 25, 2024 12:35pm - 12:45pm HST
South Pacific 1

12:35pm HST

Exhibitor Talk: Conviron
Wednesday September 25, 2024 12:35pm - 12:50pm HST
This year Conviron is launching three new products:
•             GEN1000-ECO (introduction date: April 16, 2024)
•             ConvironDirect (introduction date: March 4, 2024)
•             PGR15/E15 LED Retrofit (introduction date: Jan 5, 2024)
GEN-1000-ECO:
The GEN1000-ECO is a new compact reach in chamber ideal for short and tall plant research that comes standard with humidity control and energy efficient features such as a smaller compressor and LED lighting - for up to 15% reduced energy consumption. Low, medium and high light options are available to meet a range of research requirements.
ConvironDirect:
ConvironDirect is a new premium software tool that enables users to manage chamber setpoints and actual conditions remotely through any building LAN connected desktop, notebook or handheld mobile device. ConvironDirect is ideal for users that have Conviron reach-in plant growth chambers or walk-in rooms and want a seamless connection to their chamber, their plants, and their data from virtually anywhere.
PGR15/E15 LED Retrofit:
Fluorescent lamps such as T5, T8 and T12 have been the standard for many years and have been used in tens of thousands of plant growth chambers around the world. However, fluorescent lighting is trending towards obsolescence and replacement lights are increasingly difficult to source economically. Conviron is now offering a retrofit for aged PGR15-E15 chambers to enable users to take advantage of the latest LED lighting technology and save up to 80% on energy costs.
Speakers
CK

Caitlynn Kendrick

Account Manager, Conviron
Wednesday September 25, 2024 12:35pm - 12:50pm HST
Coral 5 - ASHS Hort Theater

12:40pm HST

CE - Interlighting Improves Tomato Yield in Northern Greenhouses
Wednesday September 25, 2024 12:40pm - 12:50pm HST
Low natural light and high heating costs limit northern winter greenhouse production. Technology advancements now offer opportunities to improve delivery of light to and within crop canopies. The greenhouse tomato cultivars Bigdena and Beorange were chosen to evaluate high pressure sodium irradiance with LED interlighting. Plants were grown in a high-wire drip irrigation system using dutch (bato) buckets (17.7 L volume) filled with a 50/50 mixture of perlite and a peatlite medium (Pro-Mix BX). The containers were placed in alternating rows across a drainpipe. Seeds were sown on 8 Sep and two seedlings were transplanted into each container 38 d later (17 Oct). Day temperature was 22 ± 2°C and reduced to 18 ± 2°C during the night. Lower leaves were removed as fruit ripened and the study was terminated at a plant height of ~250 cm (128 d from transplanting). The photoperiod was 18-h. In addition to overhead 400-W HPS lighting, LED fixtures designed for placement within the canopy were evaluated (GE current Arize® Integral). Two horizontal LED bars were positioned 30 cm (12 inches) apart with the upper bar adjusted within 30 cm of the top of the plants throughout the study. The integral lighting provided a spectrum with blue (peak 450 nm) and red (peak 660 nm) wavelengths in a 12:88 ratio. The perpendicular horizontal distance from the LED bars to the plant stems was 30 to 35 cm. The intensity (400-700 nm) measured at the plant stems horizontally from the LEDs averaged 195 ± 30 µmol m-2s-1. Overhead HPS provided ~130 ± 20 µmol m-2s-1, 100 cm below the fixtures. Seasonally short days and low sun angles limited natural light during the study. The first ripe tomatoes were harvested 62 d from transplanting (18 Dec). Interlighting resulted in higher plant yields with 6.3 ± 0.82 kg for Bigdena and 4.9 ± 0.67 kg for Beorange. In comparison, 4.1 ± 0.37 kg (Bigdena) or 3.4 ± 0.80 kg (Beorange) was recorded for plants receiving only HPS lighting. Five or six additional tomatoes were harvested with interlighting for Bigdena (25 ± 2.0 versus 19 ± 1.8) and Beorange (22 ± 2.5 versus 17 ± 1.9). Average tomato size increased from 217 ± 11.9 g to 250 ± 19.3 g (Bigdena) or from 199 ± 36.1 g to 227 ± 20.2 g (Beorange) with interlighting.
Speakers
MK

Meriam Karlsson

University of Alaska Fairbanks
Co-authors
EC

Eric Cook

University of Alaska Fairbanks
NA
Wednesday September 25, 2024 12:40pm - 12:50pm HST
Coral 1

12:40pm HST

HSMP - Exploring the Potential of Annonacin and 2-Deoxy-D-glucose on Non-Small Cell Lung Cancer Cells
Wednesday September 25, 2024 12:40pm - 12:50pm HST
Annonacin is a natural compound found in the fruit of a number of members of the Annonaceae family, including soursop and Asimina triloba. This compound has promise in targeting vital metabolic pathways, inhibiting mitochondrial complex I, and exploiting the altered energy dynamics of cancer cells that lead to apoptosis. Non-small cell Lung Cancer (NSCLC) accounts for approximately 80% of all lung cancer cases and remains a leading cause of cancer-related mortality worldwide. Despite advancements in cancer treatment, the five-year survival rate of NSCLC is low, justifying the urgent need for innovative and effective therapeutic approaches. The aberrant energy metabolism, which is a hallmark of cancer, including NSCLC, known as the Warburg effect, makes it a potential target for therapeutic interventions. In addition, 2-Deoxy-D-glucose (2DG) is a glucose analog widely studied for its ability to target the glycolytic pathway of energy metabolism of cancer. The potential combination of Annonacin and 2DG acting synergistically to inhibit the growth of A549 NSCLC cells could lead to new treatment options. The objective of this study was to examine glycolytic and mitochondrial complex I inhibitors individually and in combination to target energy metabolism to inhibit A549 NSCLC cell growth as novel antitumor agents. The study was carried out in an in vitro model system using the A549 NSCLC cell line, where the NL20 Bronchial Epithelium cell line was used as a parallel control. Cells were treated with 0 µM, 2 µM, 4 µM, and 6 µM concentrations of Annonacin and 0 mM, 2.5 mM, 5 mM, and 10 mM concentrations of 2DG, both individually and in combination in triplicate experimental design with control. The MTT assay was employed to determine immediate cell viability and assess the applied treatment's cytotoxic effects. The oxidative stress in treated and control cells was determined through superoxide dismutase and glutathione peroxidase assays. Furthermore, the long-term proliferative capacity of the cells post-treatment was examined using a colony-forming assay. The implications of the combined application of 2DG and Annonacin on A549 NSCLC cell viability and potential as a treatment of NSCLC will be discussed. These findings need further investigation to elucidate the underlying mechanisms and explore the in vivo and clinical applicability details for the combined administration of Annonacin and 2DG's use in NSCLC treatment.
Speakers
BR

Bjoj Raj Bhattarai

Kentucky State University
Co-authors
AT

Avinash Tope

Kentucky State University
NA
CT

Cora Teets

Kentucky State University
NA
KP

Kirk Pomper

Kentucky State University
Dr. Kirk W. Pomper is the Professor of Horticulture in the College of Agriculture, Community, and the Sciences at Kentucky State University in Frankfort, Kentucky. As Horticulture Research Leader, his program is focused on research and Extension efforts toward developing pawpaw as... Read More →
Wednesday September 25, 2024 12:40pm - 12:50pm HST
South Pacific 2

12:40pm HST

POM 2 - Identifying Local Apple Germplasm for Use of Hard Ciders in North Dakota
Wednesday September 25, 2024 12:40pm - 12:50pm HST
Hard cider is one of the fastest growing specialty beverages in the United States with an average annual retail sales of about $500 million in recent years. Cider apples refer to those that are specifically used for hard ciders (alcoholic) with unique traits, such as high acid, high tannin, and high total soluble solid that help ferment superior-quality hard ciders. In traditional hard cider production countries, hard ciders are made from cider-specific cultivars; however, hard ciders in the US are usually made from apples that were bred for fresh apple market because of inadequate fruit availability of cider-specific cultivars. In this study, apple germplasm grown at the NDSU Horticulture Research Farm near Amenia, ND (USDA hardiness zone 3-4a) was evaluated for the suitability of quality hard ciders. Specifically, four biochemical traits, total soluble solids (TSS), pH, titratable acidity (TA), and total phenolic compounds (TPC) that are the general attributes to the quality of hard ciders, were analyzed. Preliminary results showed that the average TSS was 15.75o Brix with the highest TSS reached 21.2o Brix. The fruit of some lines were very sour and tart indicating the high level of acidity and phenolics, which showed the great potential for quality hard cider production. The titratable acidity (malic acid) and total phenolic compounds in the apple juice of selected lines are being quantified. This research could identify local cider apple germplasm and provide apple growers and cider makers with the information on cultivar selections for quality hard cider production in ND and the surrounding region.
Speakers
WD

Wenhao Dai

Professor, North Dakota State University
Co-authors
BT

Brent Trela

Trelab
NA
MM

Mika Mzumara

North Dakota State University
NA
TS

Tian Sun

North Dakota State University
NA
ZJ

Zhao Jin

North Dakota State University
NA
Wednesday September 25, 2024 12:40pm - 12:50pm HST
Coral 2

12:40pm HST

VCM 2 - Pea-oat Green Manure and Reduced Nitrogen Rate Delay Maturity of Broccoli But Do Not Reduce Yield
Wednesday September 25, 2024 12:40pm - 12:50pm HST
Cover crop biomass can provide nitrogen (N) to a sequential cash crop as a ‘green manure’, whether the N is sourced from the atmosphere or from the soil. For short-season vegetable crops in Minnesota, like transplanted broccoli, there is time for a cover crop to be grown before or after the main crop. Growing a pea-oat cover crop early in the spring, before broccoli, may increase the N available to the broccoli. However, immobilization of green manure N may reduce N availability at a time of high N demand in broccoli. In order to study N availability to broccoli provided by a spring-planted pea-oat cover crop mix, four reduced-rate nitrogen treatments were applied to two broccoli hybrids (‘Green Magic’ or ‘Gypsy’) after a spring-planted pea-oat cover crop was incorporated. Yield and maturity of broccoli were compared to broccoli grown without green manure and given a full nitrogen rate (170 kg N / ha). The fertilizer treatments included slow-release urea (139 or 110 kg N / ha), composted poultry litter with blood meal at ~110 kg N available / ha, and turkey manure applied with blood meal in the autumn before growing green manure and broccoli (~110 kg N available / ha). There was no reduction in total yield (7900 kg / ha) or marketability (>95%), but maturity was delayed by ~2 days for most treatments. A microbial inoculant (Nature’s Source®) applied to the soil at planting did not have a measurable impact on any response.
Speakers
avatar for Charlie Rohwer

Charlie Rohwer

Scientist, University of Minnesota
Wednesday September 25, 2024 12:40pm - 12:50pm HST
South Pacific 3

12:44pm HST

Floriculture (FLOR)
Wednesday September 25, 2024 12:44pm - 1:55pm HST
Peperomia mauiensis can tolerate low indoor light conditions for up to 12 months - Orville Baldos
Investigating the Effect of Hydrafiber and Biochar As a Substitute for Peat-based Substrate for Zinnia (Zinnia elegans) - Lilin Chen
The Impact of Cold and Darkness on Osmanthus fragrans Flowering and Growth - Yulong Chen
Growth and Quality of Four Zinnia Cultivars Grown in Eastern South Dakota - Kristine Lang
Effects of Photoperiod Treatments on Stock Plants and Cutting Rooting of Callirhoe involucrata - James Klett
Consumer testing and surveys indicate a market for Peperomia mauiensis, an endemic succulent with potential use as an indoor potted plant - Orville Baldos
Trials of Low Elevation Tolerant Leucospermum Hybrids on Oahu, HI - Russell Galanti
Moderator
OB

Orville Baldos

Associate Researcher, University of Hawaii at Manoa
Wednesday September 25, 2024 12:44pm - 1:55pm HST
Nautilus

12:45pm HST

FLOR - Peperomia mauiensis can tolerate low indoor light conditions for up to 12 months
Wednesday September 25, 2024 12:45pm - 12:55pm HST
Peperomia mauiensis is an endemic peperomia found on west Maui, and the islands of Molokai and Lanai. Its short stature and reddish stems and leaves make it highly ornamental. To evaluate its feasibility as an indoor foliage plant, potted plants (n=6) were grown under three light levels (0.2 mol·m-2·d-1 [low], 0.4 mol·m-2·d-1 [office] and 2.9 mol·m-2·d-1 [high] for 12 hours) in airconditioned settings (22°C, 61% RH) for 1 year. Plant height and diameter as well as visual quality [1 = poor, 2 = fair (not saleable), 3 = acceptable (saleable), 4 = good, and 5 = excellent quality) were recorded 0, 3, 6 and 12 months after placing in light chambers. Height and diameter of plants between light treatments were similar at 0 (height = 5.9 cm; diameter = 10.5 cm) and 3 (height = 7.1 cm and diameter = 15.0 cm) months after the plants were placed in light chambers. Visual quality ratings also did not differ between light treatments at 0 (3.5) and after 3 (3.6) months. After 6 months, plants under office light conditions were the tallest (10.8 cm) and the widest (18.2 cm). Visual quality ratings between treatments at 6 months were similar (3.4). At 12 months, plant height between treatments were similar (8.8 cm), but office light plants exhibited the widest diameter (23.2 cm). Visual quality ratings between treatments at 12 months were similar (2.1) but was no longer acceptable. Results of the study indicate that Peperomia mauiensis can tolerate low indoor light levels and can be used as an indoor potted foliage plant.
Speakers
OB

Orville Baldos

Associate Researcher, University of Hawaii at Manoa
Co-authors
CW

Christyn Wong

Hawaii Department of Agriculture
NA
LW

Lindsey Watanabe

University of Hawaii at Manoa
NA
SA

Sherry Ann Hara

University of Hawaii at Manoa
NA
Wednesday September 25, 2024 12:45pm - 12:55pm HST
Nautilus

12:45pm HST

FRBR - A Census and The Conservation of Malus coronaria in Michigan
Wednesday September 25, 2024 12:45pm - 12:55pm HST
Crop wild relatives are important genetic resources for cultivar improvement. Domesticated apple (M. x domestica) cultivars grown in North America are heterogeneous hybrids of Asian and European Malus species, with no known genetic contribution from native apples. M. coronaria (Sweet Crabapple) is the only native apple species found within the major apple-producing Great Lakes region. In spite of its potential importance for apple cultivar improvement, the genetics of this species has not been well studied, and only a few accessions are maintained in the USDA National Plant Germplasm System (NPGS). The objective of this research was to identify and collect new accessions of M. coronaria for the NPGS, focusing on the northernmost extent of its range in Michigan. To locate living trees, we combined analyses of historical records in 12 herbaria and the North America Fruit and Nut Tree Data, along with crowdsourcing using the popular online platform, iNaturalist. We identified a total of 158 M. coronaria records from herbaria and the fruit tree dataset. Records were removed from consideration if they were cataloged earlier than 1950, did not include collection coordinates, were redundant, or were not collected in the state of Michigan. iNaturalist listed 309 sightings of M. coronaria in Michigan; of these, we determined that a majority were likely misidentified based on the phenotypic characteristics in the images provided by the observer. Finally, Google Earth was used to rule out locations where the M. coronaria individuals had likely been lost due to anthropogenic activity. Ultimately, this three-factored approach identified only 64 sites within the state of Michigan where a living tree would likely be found. Our results provide strong evidence that M. coronaria is in decline within its native range in Michigan, underscoring the importance of conservation efforts for this species.
Speakers
avatar for Lily Kaz Christian

Lily Kaz Christian

Graduate Research Assistant, Michigan State University
Co-authors
SV

Steve Vannocker

Michigan State University
NA
Wednesday September 25, 2024 12:45pm - 12:55pm HST
Kahili

12:45pm HST

LOCSY - Connecting the Dots: Creating School Garden and Produce Taste Testing Programs in Kentucky
Wednesday September 25, 2024 12:45pm - 12:55pm HST
More than one-third of adolescents are overweight or obese in Kentucky. Access to and consumption of fruits and vegetables is important to maintain a healthy weight and reduce disease risk; however, in Kentucky adolescents, 44.6% reported consuming fruit less than once daily and 42.7% reported consuming vegetables less than once daily. Research has shown that farm to school (F2S) activities lead to improvements in student health behaviors, including: an increase in the consumption of and preference for fruits and vegetables, and improved knowledge and attitudes to nutrition. F2S activities have been shown to have strong benefits relative to economic development, public health, education, environment, and equity and community engagement and include 3 core elements – procurement of local food by schools, agriculture, food, health, and nutrition education, and school gardens. The University of Kentucky (UK), the Kentucky Horticulture Council (KHC), Feeding KY, Community Farm Alliance (CFA), and the Kentucky Department of Agriculture (KDA) partnered to develop a Kentucky F2S Network and awarded 40 schools mini-grants to implement taste testing activities and awarded 5 schools mini-grants to establish school gardens and implement nutrition and food education in the classroom. More than 35 applications for the school garden mini-grants were submitted and 5 schools received $5,000 to install school gardens and conduct related nutrition and cooking education. UK student interns assisted schools and conducted garden/nutrition lessons, reaching over 2,000 students. Garden grantees grew and harvested 35 fruits and vegetables. During the final project reporting period, grantees engaged over 2,000 people in gardens for a total of 3,000 hours collectively spent in the garden with 1,300 of those hours coming from parent and community member involvement. Interns presented 3 school garden educator workshops and developed resources about best practices for school gardens. Over 100 applications were received for $1,000 taste test mini-grants and 40 grants were awarded directly to KY schools to conduct taste test activities of locally-grown fruits and vegetables. The taste testing grantees: purchased $24,369 in produce from 90 Kentucky farms; served 70 different types of fruits and vegetables; and served locally-grown produce to 11,000 students in 55 different schools. The presentation will focus on strategies used to recruit and screen program participants by assessing needs, setting realistic expectations, and increasing production knowledge and skills, with the goal of influencing student food choices and increasing use of locally-grown produce in classroom education and school feeding programs.
Speakers
CF

Cindy Finneseth

Kentucky Horticulture Council
Co-authors
KO

Kendra OoNorasak

University of Kentucky
NA
MF

McKenzie Fox-Potter

Kentucky Horticulture Council
NA
Wednesday September 25, 2024 12:45pm - 12:55pm HST
South Pacific 1

12:45pm HST

PB 2 - Rapid Detection and Coinfection Analysis of Aspergillus flavus in Peanut Seeds
Wednesday September 25, 2024 12:45pm - 1:00pm HST
Aspergillus flavus is a widespread pathogen affecting crops like peanuts, contributing significantly to mycotoxin contamination and subsequent crop losses. Discriminating between toxigenic and non-toxigenic strains is crucial, yet conventional methods are often cumbersome and time-consuming. In this study, we developed rapid molecular tools to differentiate between these strains. Using morphological characteristics and species-specific PCR-sequencing, we identified isolates collected from peanut seeds in southern Georgia. Through primer optimization and qPCR targeting aflatoxin regulatory genes, we successfully distinguished aflatoxin-producing and non-producing isolates. Additional genes involved in aflatoxin biosynthesis were also analyzed, showing clear expression distinctions. Our findings demonstrate the specificity and efficiency of these primer sets, providing a valuable tool for managing A. flavus contamination in peanut seed lots. Additionally, research on the seed microbiome's impact on mycotoxin production remains limited. In this study, we assessed microbial communities in peanut seeds collected over various years using ITS gene sequencing. Our results revealed a diverse microbial population, including A. flavus and other fungal pathogens, highlighting the complexity of seed microbiota. This approach offers novel insights into peanut seed-associated microbiomes and aflatoxin contamination, shedding light on the correlation between microbial communities and aflatoxin pollution.
Speakers
EA

Emran Ali

Alcorn State University
Co-authors
SW

Sumyya Waliullah

Alcorn State University
NA
VN

Victor Njiti

Alcorn State University
NA
Wednesday September 25, 2024 12:45pm - 1:00pm HST
South Pacific 4

12:45pm HST

UG - A Comparative Study Analyzing Light Lengths for the Growth of Rex Butterhead Lettuce Utilizing GREENBOX Technology
Wednesday September 25, 2024 12:45pm - 1:00pm HST
Exponential population growth adds increasing pressure on the agriculture industry to grow and distribute fresh foods to rural and urban areas, leading to the development of GREENBOX technology, which utilizes Controlled Environment Agriculture (CEA) principles to optimize the desired conditions for growth of leafy green crops. Using commercially available equipment, GREENBOX technology has the capability to be integrated into existing urban infrastructure to help relieve the negative impact urbanization has on the availability of fresh foods. GREENBOX technology allows environmental variables, such as temperature, humidity, light intensity/ spectrum, and nutrient delivery, to be controlled to enhance the growth performance of leafy greens. Precursory experiments using GREENBOX Technology utilized the standard photoperiod of 16 hours of light, and 8 hours of dark for all crop production. The main objective of this study was to conduct a comparative analysis of Lactuca sativa Rex Butterhead Lettuce production grown under different photoperiods using GREENBOX technology. Using the standard 16 hours of light and 8 hours of dark as the control, two different photoperiod treatments were tested. Treatment one consisted of a 14-hour light period and a 10-hour dark period, while treatment two consisted of a 12-hour light period and a 12-hour dark period. The biomass and productivity of the crops were measured in addition to the environmental conditions for each lighting regimen to ascertain any significant differences. The biomass parameters included wet weight (g), dry weight (g), leaf area (cm2), leaf count (n), and chlorophyll concentration (mg/cm2). We derived additional data, including the Leaf Area Index (LAI, cm2/cm2), Specific Leaf Area (SLA, cm2/g), and biomass productivity (kg/m2). A statistical analysis of the biomass data was used to understand the differences in biomass parameters between crop growth and different light lengths. No statistically significant differences were found between the biomass and productivity parameters for the 12-hour and 14-hour photoperiods. However, the weight weights, dry weights, Leaf Count, SLA, and LAI from the 16-hour photoperiod showed statistically significant differences from the 12 and 14-hour photoperiods. All treatments still produced Rex Butterhead lettuce above the expected harvest weight of 180g and are viable for crop production in urban warehouse settings. The results of this experiment may help us understand the relationship between photoperiod and the biomass performance of leafy greens. Future GREENBOX experiments may use this information to increase the efficiency and productivity outputs of GREENBOX units. Keywords: CEA, Hydroponics, lettuce, soilless agriculture, urban agriculture
Speakers
MG

Mya Griffith

Florida Gulf Coast University
Co-authors
AS

Ankit Singh

University of Maine
NA
BR

Barry Rosen

Florida Gulf Coast University
NA
GP

Galen Papkov

Florida Gulf Coast University
NA
GB

George Buss

Florida Gulf Coast University
JG

John Griffis

Florida Gulf Coast University
NA
KJ

Kathryn Jackson

Independent Researcher
NA
PC

Paige Carroll

Florida Gulf Coast University
NA
SB

Sarah Bauer

Mercer University
NA
XY

Xiusheng Yang

University of Connecticut
NA
Wednesday September 25, 2024 12:45pm - 1:00pm HST
Lehua Suite

12:50pm HST

CE - Effect of low root-zone temperature and UV radiation on growth and gene expression of secondary metabolite pathways in N
Wednesday September 25, 2024 12:50pm - 1:00pm HST
This study explored the effects of low root-zone temperature (LT) and UV radiation (UV) alone and combined on changes in growth, transcription, and gene expression related to secondary metabolite in Nicotidana benthamiana. The plants were grown in a controlled environment (25/20°C, 16/8 h [light/dark], 70% relative humidity, 1,000 µmol·mol−1 CO2 with photosynthetic photon flux densities of 100 and 200 µmol·m−2·s−1 for 10 and 18 d, respectively). Twenty-eight days after sowing, the seedlings were treated with LT (15°C), 0.3 W·m−2 of UV radiation, and a combined treatment with LT and UV (LT*UV) for 3 d. Results found that the treatment with UV alone decreased the quantum efficiency of photosystem II by approximately 1.5 times, and most growth characteristics decreased under the UV (approximately 1.5 times) and LT*UV treatments. Combined treatment with LT*UV significantly inhibited the growth characteristics and photosynthetic rates compared to those under the single LT and UV treatments. In particular, the transcriptome levels of phenylpropanoid and flavonoid biosynthesis were the most significantly affected by LT*UV. This suggests the potential of using LT treatment in hydroponic systems and UV radiation to control the synthesis of health-promoting compounds of secondary metabolites in greenhouses and controlled-environment agricultural facilities.
Speakers
avatar for KiHo Son

KiHo Son

Gyeonsang National University
Co-authors
EG

Eiji Goto

China university
NA
Wednesday September 25, 2024 12:50pm - 1:00pm HST
Coral 1

12:50pm HST

HSMP - Responses and relationship between bioactive compounds and phenotypic traits in Dahurian angelica breeding lines
Wednesday September 25, 2024 12:50pm - 1:00pm HST
Dahurian angelica (Angelica dahurica (Fisch. ex Hoffm.) Benth.
Speakers
EJ

Eun Jeong Koh

Gyeongsangbuk-do Agricultural Research
NA
Co-authors
BS

Beong Sung Kim

Gyeongsangbuk-do Agricultural Research
NA
JK

Jiwon Kim

Gyeongsangbuk-do Agricultural Research
NA
JH

Joong Hwan Lee

Gyeongsangbuk-do Agricultural Research
NA
Wednesday September 25, 2024 12:50pm - 1:00pm HST
South Pacific 2

12:50pm HST

POM 2 - The Fruitlet Size Distribution Model As A Thinning Decision Aid For Precision Crop Load Management Of Apple
Wednesday September 25, 2024 12:50pm - 1:00pm HST
Fruit quality of apple is heavily influenced by crop load management practices. During the developmental window between bloom and 15mm fruitlet diameter, the general commercial practice is to apply chemical thinners to reduce the initial crop load based on the cultivar and market. However, thinning success varies from year to year, depending on bloom density, thinner selection and dose, climatic factors, tree health and cultivar. Precision crop load models such as the pollen tube growth model, the MaluSim model, and the fruit growth rate model have improved thinning success; however, labor-intensive measures has hindered broad adoption of the latter model which effectively predicts fruit set following thinner application. To improve grower adoption, we developed an alternative, time-efficient fruit set prediction model. The model has been tested on multiple cultivars over a three-year period at four distinct apple production regions throughout the United States. Abscission probabilities and fruit set predictions are based on the distribution of fruitlet mass and confirmed by actual measures of fruit set. The model starts at 6 mm fruitlet diameter and accounts for all thinners up to this time. A prediction is returned in ~8 days to inform repeat thinner applications while fruitlets are still highly sensitive to thinners. A user-guide, digital balance that automatically exports fruit mass to a laptop/tablet, and a macro-enabled Excel spreadsheet file have been developed and are available to growers to increase the precision around crop load management.
Speakers
LH

Laura Hillmann

Michigan State University
Co-authors
SS

Sara Serra

Washington State University
NA
SM

Stefano Musacchi

Washington State University
NA
TR

Terence Robinson

Cornell University
NA
TE

Todd Einhorn

Michigan State University
NA
TK

Tom Kon

North Carolina State University
NA
Wednesday September 25, 2024 12:50pm - 1:00pm HST
Coral 2

12:50pm HST

VCM 2 - Field Screening of Cabbage (Brassica oleracea var. capitata) Cultivars for Resistance to Black Rot
Wednesday September 25, 2024 12:50pm - 1:00pm HST
One of the most effective management strategies for controlling black rot (Xanthomonas campestris pv. Campestris (Xcc)) in cabbage (Brassica oleracea var. capitata) is resistant cultivars. The objectives of this research were to evaluate commercial and experimental cabbage cultivars for black rot resistance and determine yield potential, harvest maturity, and head quality. A field experiment with nine cultivars (1488, Capture, Celebrate, Cheers, Expat, Melissa, TCA-576, TCA-606, and TCA-607) was carried out during the fall season of 2023 at Hort Hill research farm on the University of Georgia, Tifton campus. ‘Cheers’ (commonly grown high-yielding cultivar), ‘Capture’, and ‘Expat’ (claims high resistance to black rot), and ‘Melissa’ (black rot susceptible Savoy cabbage) were used as checks for comparison with other F1 cabbages. Plants were spray-inoculated with Xcc (250 ml of 10^6 CFU/ml) at 5 and 7 weeks after transplanting. Treatments were arranged in a randomized complete block design with four replications. Relevant agronomic practices, such as irrigation, fertilization, and insect management, were implemented uniformly across all plots. Black rot severity was rated using a 1-9 scale, where 1 indicates the most resistant and 9 represents the least resistant (most susceptible) cultivar. The trial was harvested four times on the following dates:11/30/2023, 12/12/2023, 12/20/2023, and 01/03/2023. Black rot severity, total head counts and weights; and average head weight and height: and average core height, and width were statistically significant between treatments. ‘TCA-607’ and ‘Capture’ had the highest resistance to black rot disease, while ‘1488’ was the most susceptible. All other cultivars expressed moderate levels of resistance. In the first harvest ‘Cheers’ and ‘TCA-607’ had the highest total marketable head counts and weights, indicating early maturity. In addition, ‘Cheers’ and ‘TCA-607’ cabbage had the highest total marketable head counts and weight, followed closely by ‘TCA-606, ‘Celebrate’, and ‘1488’. ‘Cheers’ and ‘TCA-607’ had the highest average head height. Furthermore ‘TCA-607’ outperformed all the cultivars for the average head weight. Among all the cultivars ‘Melissa’ and ‘Expat’ performed the worst for total marketable counts and weights. Overall based on our trial, the experimental cultivar ‘TCA-607’ has the best combination of traits: best black rot resistance, highest yields, and biggest heads.
Speakers
avatar for Manisha Kumari

Manisha Kumari

Post-Doctoral Research Associate AD, The University of Georgia
Manisha Kumari is a post-doctoral research associate AD at Horticulture department, University of Georgia, Tifton campus, Tifton 31793, GA, USA.
Co-authors
TM

Ted McAvoy

University of Georgia
NA
Wednesday September 25, 2024 12:50pm - 1:00pm HST
South Pacific 3

12:55pm HST

FLOR - Investigating the Effect of Hydrafiber and Biochar As a Substitute for Peat-based Substrate for Zinnia (Zinnia elegans) and Snapdragon (Antirrhinum majus) Production
Wednesday September 25, 2024 12:55pm - 1:05pm HST
The increasing environmental concerns and economic pressures necessitate the search for peat moss alternative as a substrate component, aiming to balance ecological sustainability with cost-effectiveness. This study aims to assess whether the biochar (BC) and hydrafiber (HF) could be a partially replacement for peat moss for zinnia and snapdragon production. Twelve substrates were formulated by either mixing BC (20%,40%, or 60% by vol) with HF (20%, 40%, or 60% (by vol), with the remaining being peat moss or blending BC (0%, 20%,40%, and 60%, by vol) with the commercial peat moss-based substrates (CS). The physical properties of substrate, including container capacity (CC), total porosity (TP), air space (AS), bulk density (BD), and water holding capacity (WHC), and chemical properties including leachate pH and electrical conductivity (EC) were measured. Plant growth parameters including growth index (GI) and leave greenness (indicated with SPAD value), biomass, and numbers of flowers were measured biweekly. The results showed all the substrate mixes had similar AP, BD and SPAD. Treatment with 20%BC and 80%CS had the highest plant growth, biomass, and numbers of flowers for both zinnia and snapdragon plants. In conclusion, BC could be used to partially (20%) replace commercial peat moss-based substrate mix for container-grown zinnia and snapdragon production.
Speakers
avatar for Lilin Chen

Lilin Chen

Graduate Research Assistant, University of Georgia
I'm interested in biochar effect on containerized plants, especially the effect under abiotic stresses such as drought and salinity.
Co-authors
PY

Ping Yu

University of Georgia
YC

Yulong Chen

The University of Georgia
Wednesday September 25, 2024 12:55pm - 1:05pm HST
Nautilus

12:55pm HST

LOCSY - Garden to Pantry: An Interactive and Interdisciplinary Approach to Growing and Preserving Produce
Wednesday September 25, 2024 12:55pm - 1:05pm HST
Garden to Pantry: An Interactive and Interdisciplinary Approach to Growing and Preserving Produce Rebecca C. Lee1 and Carmen Flammini2, 1 Alabama Cooperative Extension Service, Jon Archer Agricultural Center, 1070 N. Schillinger Rd., Mobile, AL 36608, 2Alabama Cooperative Extension Service, 302A Byrne St., Bay Minette, AL 36507 Recent research conducted post-COVID-19 indicates that households experiencing food insecurity have escalated their home food procurement activities, including gardening, fishing, canning, and food preservation. These studies propose that transitioning procurement activities into sustainable lifestyles and enhancing fruit and vegetable consumption among food-insecure populations will necessitate technical support and outreach initiatives like Garden to Pantry (G2P). Home gardening and food preservation programs can play a vital role in educating communities about fruit and vegetable cultivation and preservation. The objectives of the Alabama Cooperative Extension Service (ACES) program entitled ‘Garden to Pantry: An Interactive and Interdisciplinary Approach to Growing and Preserving Produce’ aimed to deliver a complete and immersive program from planting to preserving of seasonal fruit and vegetables in a one-day program by offering research-based recommendations for methods of home food preservation. Additionally, the program aimed to provide the community with cross-discipline knowledge and experience from varying disciplines. The pilot program of the ‘Garden to Pantry: An Interactive and Interdisciplinary Approach to Growing and Preserving Produce’ program was presented in five counties in Alabama in 2023 and 2024. Data obtained during the 2023 run of the program revealed the program was positively received by the target community and further interest was expressed in participating in other programs focusing on food safety as a whole concept by integrating all aspects of food preservation. Future challenges of this program include seeking involvement with younger stakeholder demographics as well as individuals interested in the increasing popular homesteading movement.
Speakers
CF

Carmen Flammini

Alabama Extension System, Auburn University
avatar for Rebecca Catalena

Rebecca Catalena

Food Safety & Quality, Alabama Extension
Rebecca Catalena is a Regional Extension Agent in Food Safety & Quality. Rebecca received her Master’s in Public Health (MPH) from the University of West Florida and an A.A.S degree from Le Cordon Bleu. Before joining the Alabama Extension family, Rebecca served seven years in... Read More →
Co-authors
DR

Dani Reams

Alabama Extension
NA
Wednesday September 25, 2024 12:55pm - 1:05pm HST
South Pacific 1

12:55pm HST

Exhibitor Talk: Moss Robotics
Wednesday September 25, 2024 12:55pm - 1:10pm HST
Wednesday September 25, 2024 12:55pm - 1:10pm HST
Coral 5 - ASHS Hort Theater

1:00pm HST

CE - Effects of Short-Wavelength UV-B, UV-A, and Blue Light on the Rose Flower Color Transition Phenotype
Wednesday September 25, 2024 1:00pm - 1:10pm HST
Light quality plays a crucial role in the growth and development of plants. In this study, we aimed to assess the effects of short-wavelength light on rose genotype '16401-N055’, which exhibits the flower color transitioning trait: the flowers change colors from yellow to pink in sunlight. Roses that exhibit this flower phenotype are termed transitioning-type roses. Specifically, we analyzed and compared the impact of six different light treatments [a sunlight control in open field and five spectral treatments created using light-emitting diodes (LEDs)] on various physiological and morphological characteristics. The five LED treatments included white light, blue light, UV-A white light, UV-B white light, and 80% blue 20% white light. Each treatment had two replications where one-year-old rose plants were the experimental unit. The total light intensity was maintained at 300-350 micromol m-2 s-1 for a photoperiod of 16 h light and 8 h darkness. The morphological traits measured included height, width, number of buds, number of flowers, and node density. The color scale parameters L* [luminance of the color ranging from 0 (black) to 100 (white)], a* [red (positive values) and green (negative values) color levels], and b* [yellow (positive values) and blue (negative values) color levels] were measured using a colorimeter. Additionally, the chlorophyll concentration index (CCI) was measured using a chlorophyll meter. Fully pigmented pink flowers were only observed in the UV-B white light treatment with an average L* value of 44.1 and a* value of 50.6. A slight pink hue was observed on the outer sections of petals in blue and blue white light treatments. The average L* and a* values of flowers in the blue treatment were 87.21 and 6.24, and in blue white treatment were 90.9 and 1.86, respectively. The flowers in the white UV-A treatment remained white with the highest average L* value of 92.4 and the lowest average a* value of -1.86. The CCI of plants under sunlight (23.5) was significantly lower than the plants treated with blue (34.4), and blue white (33.7) light. No significant differences in morphological traits were detected after two weeks. The plants will be monitored for longer periods and more data will be collected every two weeks for one month to document additional changes. The results obtained will provide additional information on morphological and floral changes in this genotype under different light treatments.
Speakers
NK

Navdeep Kaur

Texas A and M university
Co-authors
DB

David Byrne

Texas A and M University
NA
OR

Oscar Riera-Lizarazu

Texas A and M University
NA
SZ

Shuyang Zhen

Texas A and M University
NA
Wednesday September 25, 2024 1:00pm - 1:10pm HST
Coral 1

1:00pm HST

HSMP - Effects of Foliar Application of Humic Acid and NPK Fertilizers to the Soil on Important Growth Indices of Black Mustard
Wednesday September 25, 2024 1:00pm - 1:10pm HST
Humic acid that improves plant growth and yield indices such as fresh and dry weights, plant height. Field experiment was conducted at the Al-Mussaib Technical Institute/Iraq during the 2022-2023 season to study the effect of adding NPK complex fertilizer to the soil and spraying with organic fertilizer (Humic acid) on the growth and yield of black mustard plants, according to a randomized complete block design (RCBD) with three replications. In this study, the effect of foliar application of Humic acid and/or fertilizers of NPK on the growth characteristics of black mustard (Brassica nigar) in field conditions were compared. According to the results, the foliar application of Humic acid The results were analyzed according to analysis of variance, and the means were compared using the least significant difference (LSD) test under the probability level of 0.05.A two-factor experiment (3×3) was carried out. The first factor was the addition of a balanced NPK complex fertilizer (20:20:20). At levels of (0, 30, or 60) kg/dunum in two batches, the first two weeks after germination, and the second when flowering begins. The fertilizer was placed about 10 cm under the plant in the furrows and covered with dirt, then watered after fertilization. The second factor was spraying with three concentrations of organic fertilizer (Humic acid) (0, 2, or 4) ml.l-1. It was observed from the results that adding NPK complex fertilizer and organic fertilizer (Humic acid) has a significant effect on the characteristics of vegetative growth, as is evident from the fact that the levels of NPK complex fertilizer and spraying with organic fertilizer (Humic acid) have a significant effect on the productive characteristics of the plant. It is conclude from this experiment that the most effective treatment that can be used to feed the black mustard plant under the conditions of this experiment, and which achieved the best results in improving the vegetative and productive characteristics of the plant, is the 60 kg overlap treatment. 1 dunam-1 NPK with spraying three times with 2 ml.l-1 of organic fertilizer (Humic acid), which improved the characteristics of vegetative growth and gave the highest rate of mustard number. Plant-1 and number of seeds. Mustard-1 and seed weight. Plant-1 and the amount of seed yield per dunum. application of 45 ppm humic acid with 60/kg – per dunum (per 2500 square meters) would be the best option to maintain better growth, yield and quality in black mustard.
Speakers
KA

Keefah AL-Garallaa

Mississippi State University
Co-authors
avatar for Richard Harkess

Richard Harkess

Mississippi State University
NA
Wednesday September 25, 2024 1:00pm - 1:10pm HST
South Pacific 2

1:00pm HST

POM 2 - Deciphering the Proline-Mediated Flowering Delay Mechanism in Peach through Physiological and Metabolic Analyses
Wednesday September 25, 2024 1:00pm - 1:10pm HST
Freezing temperatures can harm peaches in late spring with advanced flowering due to climate change and consequently reduce their yield. Flowering delay is a prominent strategy employed to avoid such spring frost damage. Our previous study demonstrated that treatment with 5% sodium alginate 100 mM CaCl 2 (5AG) can delay the blooming, potentially avoiding frost damage. To elucidate the precise mechanism of flowering delay induced by 5AG in peaches, this study systematically and experimentally analyzed the changes of amino acid profiles in control and 5AG-treated peach plants at different day intervals (0, 1, 2, 11, 21, 25, and 28 DAT). Our findings revealed that the levels of arginine (Arg), glutamate (Glu), and proline (Pro) differed significantly between control and 5AG- treated peach shoots throughout the phenological development of flower buds. Furthermore, Arg and Glu amino acids, are involved in the Pro pathway. Computational metabolomics analyses identified the Pro metabolism related genes and their attributes, gene ontology, gene synteny and gene organizations, which represent diverse biological function of these genes, including flowering responses. In addition, qRT-PCR analysis results revealed that elevated expression of P5CS and P5CR genes, involved in Pro metabolism, led to increased Pro content in control plants. Conversely, 5AG treatment downregulated these genes, resulting in lower proline content. This result suggests that 5AG treatment may restrain gene expression related to Pro accumulation, thereby controlling Pro biosynthesis. In addition, our findings unveiled a direct connection between Pro content reduction and delayed flowering. Taken together, these results provide strong evidence that 5AG treatment significantly delays flowering by controlling Pro metabolisms in Prunus persica.
Speakers
avatar for HYUNSUK SHIN

HYUNSUK SHIN

Gyeongsang National University
Co-authors
JH

JAE HOON JEONG

Gyeongsang National University
NA
MP

MUTHURAMALINGAM PANDIYAN

Gyeongsang National University
NA
SH

Seung Heui Kim

Korea National College of Agriculture and Fisheries
NA
YP

YEONJU PARK

Gyeongsang National University
NA
Wednesday September 25, 2024 1:00pm - 1:10pm HST
Coral 2

1:00pm HST

VCM 2 - Evaluation of Brassica Oleracea Genotypes in Terms of Agronomic Performance
Wednesday September 25, 2024 1:00pm - 1:10pm HST
Plant growth performance of 35 different broccoli cultivars was assessed during the Fall season 2023 in terms of plant growth and head development in SW Florida. In particular, seeds of Brassica oleracea var. italica genotypes that were obtained from USDA, ARS, Plant Genetic Resources Unit (PGRU)
Speakers
ST

Sotirios Tasioulas

SWFREC - University of Florida/IFAS
Co-authors
JW

Jessie Watson

SWFREC - University of Florida/IFAS
NA
PT

Pavlos Tsouvaltzis

Southwest Florida Research and Education Center, University of Florida
NA
TL

Tie Liu

University of Florida
NA
Wednesday September 25, 2024 1:00pm - 1:10pm HST
South Pacific 3

1:00pm HST

PB 2 - RNA-seq Analysis Reveals Differentially Expressed Genes in Sweetpotato cv. Beauregard under Lead Stress
Wednesday September 25, 2024 1:00pm - 1:15pm HST
Lead (Pb) is a widespread toxic element in agricultural soils and Pb accumulation in plant roots represents a potential health risk for human beings. The sweetpotato (Ipomoea batatas L.) is a globally important root crop and one of the leading raw products for baby food processing. Limited information is available about the mechanism by which sweetpotato responds to Pb stress at the molecular level. Understanding the genetic mechanism of Pb uptake is essential for developing management approaches to mitigate Pb uptake in this crop. To address this knowledge gap, RNA-seq was used to characterize the transcriptome and identify differentially expressed genes from Pb-treated and untreated sweetpotato cv. Beauregard. Samples were taken from adventitious root tips at 5, 10, and 15 days after planting (DAP). Transcriptomic analysis revealed 4,077, 5,159, and 3,206 differentially expressed genes at 5, 10, and 15 DAP respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis shows that ABC transporters and sulfur metabolism pathways are upregulated at 5 DAP but are downregulated at 15 DAP, indicating that there may be a threshold in sweetpotato Pb tolerance. The results provide a deeper insight into the species-specific response of sweetpotato to Pb stress which can lead to the development of screening methods and evaluation of management strategies that reduce Pb uptake in this crop.
Speakers
MA

Mary Ann Munda

Louisiana State University
Co-authors
AV

Arthur Villordon

LSU Agcenter
DL

Don La Bonte

Louisiana State Univ
NA
LA

Lisa Arce

Louisiana State University
MA

Mae Ann Bravo

Louisiana State University
MB

Marissa Barbosa

Lousiana State University
Wednesday September 25, 2024 1:00pm - 1:15pm HST
South Pacific 4

1:05pm HST

FLOR - The Impact of Cold and Darkness on Osmanthus fragrans Flowering and Growth
Wednesday September 25, 2024 1:05pm - 1:15pm HST
Osmanthus fragrans, a culturally and economically significant plant in Asian countries, exhibits unique floral characteristics influenced by endogenous metabolites. Previous studies suggest its flowering timing and intensity may be affected by environmental factors including cold and darkness. This study aimed to investigate the effects of cold and darkness factors on the response of Osmanthus fragrans flowering and growth. This experiment included 7 cold and dark treatments (plants were sitting in the dark coolers from 0-15 days) and dark-only treatments (plants were sitting in the GH with plastic bags from 0-9 days). Plant responses were assessed based on the number of flowers, height, and width measurements on the 5th, 7th, 9th, 11th, 13th, and 15th days post-treatment. The results showed there was no significant difference in plant height across treatments. Dark-only treatment for 7 days significantly increased width growth compared to the control group (9 days), and dark-cold treatment for 5, 7, 9, 11, and 13 days. The increase in the number of flowers in the cold-dark treatment for 9 and 15 days was significantly higher than in other treatments, except for the control. In conclusion, the cold and darkness could promote flowering in Osmanthus fragrans without negatively affecting plant growth.
Speakers
avatar for Yulong Chen

Yulong Chen

The University of Georgia
Co-authors
LC

Lilin Chen

The University of Georgia
NA
PY

Ping Yu

University of Georgia
Wednesday September 25, 2024 1:05pm - 1:15pm HST
Nautilus

1:05pm HST

LOCSY - Fermented Hot Pepper Sauce as a Model for New Value-Added Opportunities for Local Food Production
Wednesday September 25, 2024 1:05pm - 1:15pm HST
Development of value-added products by local growers can lead to additional economic opportunities for small farmers by utilization of unsold crops for production of products. Kentucky State University (KSU) received a USDA Capacity Building grant to create a Fermentation and Distillation Sciences Program to train students in this area, but also help local farmers develop new value-added fermented products. There are many job opportunities either supporting or directly involved in the fermentation and distillation sciences in Kentucky and the surrounding region. The goal of the program is to develop new recipes for value-added products for small-scale producers, including pawpaw, hemp, and sorghum beer and kombucha, spirits, and fermented fruit and vegetable products through research supported by new equipment. Hot pepper sauce is of great interest to small producers since it is fairly easy to make and there are many variations in recipes. The objective of this current study was to develop new recipes for fermented hot pepper sauce as new value-added products for small-scale producers. Pepper plantings were established at the Kentucky State University Harold R. Benson Research and Demonstration Farm for a range of pepper cultivars including: Shishimai (Shishito Pepper), Sargento (Poblano Pepper), Bottle Rocket (Cayenne Pepper), Mad Hatter (Bishop's Crown Pepper), Roulette (Habanero Pepper), Helios (Habanero Pepper), Carmen (Corno di Toro Pepper), and a number of other peppers over a three year period. Initially, a number of recipes for fermented hot pepper sauce were evaluated and subject to taste trails. These recipes varied in preparation, pepper type, and vinegar addition. Finally, a potential unique fermented hot pepper sauce was developed that would be of interest to local farmer to produce as a value added product. A tasting trail was conducted with 23 individuals on the KSU campus comparing this new recipe to three commercially available hot sauces. There were 14 males and 8 female participants, with 1 participant preferring not to answer. The average age range was between 26 and 45. Hot sauce samples were served on saltines and evaluated from 1 to 5 for color, smell, flavor, heat, and willingness to pay. The individuals were also asked to rank their favorite sauce. Additional data was collected concerning the participants liking of spicy foods. The fermented hot pepper sauce recipe that was developed was well received and should be of interest for production by local farmers.
Speakers
MB

Matthew Behrends

Kentucky State University
NA
Co-authors
JL

Jeremy Lowe

Kentucky State University
JN

Joni Nelson

Kentucky STate University
KP

Kirk Pomper

Kentucky State University
Dr. Kirk W. Pomper is the Professor of Horticulture in the College of Agriculture, Community, and the Sciences at Kentucky State University in Frankfort, Kentucky. As Horticulture Research Leader, his program is focused on research and Extension efforts toward developing pawpaw as... Read More →
SC

Sheri Crabtree

Kentucky State University
Wednesday September 25, 2024 1:05pm - 1:15pm HST
South Pacific 1

1:10pm HST

CE - Arduino Uno Can Reliably Log Substrate Moisture from a Bus of Digital Sensors and Control a Drip-Irrigation System
Wednesday September 25, 2024 1:10pm - 1:20pm HST
Inexpensive Arduino microcontrollers can be programmed to operate and log data from environmental sensors and operate other accessories such as irrigation solenoids. We describe our efforts to build a modified version of Arduino Uno systems previously developed at the University of Georgia, which operated analog moisture sensors and opened solenoid valves to drip emitters when moisture fell below user-defined thresholds. We attempted to 1) replace analog sensors with a bus of digital sensors that use the SDI-12 communication protocol, 2) include programming to parse digital output from two popular SDI-12 sensors (Decagon GS3 and Campbell Scientific 5TM), 3) use 12VDC solenoid valves that were less expensive and smaller (1/2”) than alternatives, and 4) overcome several challenges encountered in the construction and programming of the Arduino-based system. These included an approach to more easily manage the connection of numerous wires, the inclusion of a reversed diode at the solenoid terminals to prevent electrical interference from intermittently resetting the Arduino program, and the adoption of programming strategies to work around memory limitations that initially rendered our Arduino systems with digital sensors unreliable. We overcame these challenges to develop a robust, reliable, and easy-to-deploy Arduino-based environmental logger and automated drip-irrigation system that can operate numerous digital sensors. Sensor type and thresholds for volumetric water content are defined in a single location within the program, enabling the user to easily make minor adjustments to the system. We also included extensive line-by-line documentation of the source code. A list of the hardware used in this system is available. In 2023, eight of these systems operating 64 total sensors proved their reliability over a two-month experiment on the drought stress physiology of wetland shrubs. We conclude that this system is an effective solution for in-house sensor-automated irrigation with high customizability for end users.
Speakers
SE

Stephanie E. Burnett

University of Maine
NA
Co-authors
AJ

Adam J. Peterson

Unaffiliated
NA
BP

Bryan Peterson

University of Maine
JH

Jessica Hutchinson

University of Maine
RS

Rhuanito S. Ferrarezi

University of Georgia
NA
Wednesday September 25, 2024 1:10pm - 1:20pm HST
Coral 1

1:10pm HST

HSMP - Assessment of micropropagated ginger cultivars and generations on plant growth and rhizome yield in a greenhouse
Wednesday September 25, 2024 1:10pm - 1:20pm HST
Our research demonstrated micropropagated ginger helped solve seed ginger sourcing issue for this “niche” market crop. Traditionally, farmers would save ginger rhizomes from the previous year’s harvest, store over winter, and then use as seeds for the upcoming growing season. This research was to determine if micropropagated ginger generations would affect ginger growth and yield. We studied nine ginger cultivars (BB, BG, BK, CW, HY, MD, PY, KY, and KM) from various tissue culture (TC) generations (TCF1, TCF2, TCF3, and TCF4). TCF1 seedlings were initially planted (March 2023), and then transplanted with TCF2, TCF3 and TCF4 from previous years’ study, into individual grow bags (May 2023) with one seedling per 10-inch grow bag (2:1 metro mix:compost). Plants were placed on a bench inside a greenhouse with a completely randomized design (CRD) with 20 cultivar/TC Generation combinations, 5 replications per cultivar/generation. We collected growth data (stem number, diameter, length, and SPAD), yield data (number of rhizome pieces per seedling, weight of individual rhizome piece/finger, weights of biological root, edible root and total rhizome weight), and PAR of individual plants. Data was then analyzed using SAS OnDemand for Academics with PROC GLM at the 0.05 level of significance. There was a significant effect as the number of TC generations increased, with stem length (TCF1 – 91.8, TCF2 – 91.0, TCF3 – 103.5, and TCF4 – 101.5 cm) and stem diameter (TCF1 – 6.2, TCF2 – 6.4, TCF3 – 8.1, and TCF4 – 8.1 mm) increasing, while number of stems (TCF1 – 19.0, TCF2 – 18.3, TCF3 – 10.4, and TCF4 – 9.9) decreased on average. TC generations had significant effect on rhizome yield. Individual pieces of ginger rhizome decreased in number (TCF1 – 37.9, TCF2 – 31.1, TCF3 – 28.9, and TCF4 – 28.0) but increased in size (weight) (TCF1 – 12.3, TCF2 – 16.8, TCF3 – 25.4, and TCF4 – 23.1 g) as number of TC generations increased. Similarly, biological root weight significantly decreased (TCF1 – 175.9, TCF2 – 195.3, TCF3 – 42.0, and TCF4 – 52.9 g). Edible root weight significantly increased (TCF1 – 443.9, TCF2 – 460.6, TCF3 – 700.6, and TCF4 – 641.0 g). There was a slight upward trend of rhizome yield as TC generations increased (TCF1 – 619.9, TCF2 – 671.3, TCF3 – 761.2, and TCF4 – 709.5 g).
Speakers
GY

Guochen Yang

Professor and Graduate Program Coordinator, Oversight Coordinator for University Hemp Program, North Carolina A&T State University
Co-authors
DT

Daniel Tetteh

North Carolina A
NA
JR

Julia Robinson

North Carolina A
NA
WL

William Lashley

North Carolina A
NA
ZM

Zipporia Moore

North Carolina A
NA
Wednesday September 25, 2024 1:10pm - 1:20pm HST
South Pacific 2

1:10pm HST

POM 2 - Genetic Architecture of Important Traits for Prunus Crop Improvement
Wednesday September 25, 2024 1:10pm - 1:20pm HST
Numerous QTL and GWAS studies have been conducted on various species of Prunus, but there is limited knowledge on the overall genetic architecture regulating fruit quality traits among these species. As part of a 2022-funded SCRI- project to advance database resources for specialty crop research, efforts are directed towards collecting and curating all types of big data in the Genome Database for Rosaceae (www.rosaceae.org). We conducted an extensive literature review on over 120 QTL and GWAS studies on Prunus traits to extend the existing data in GDR. We aligned the traits to the peach genome to compare important traits in various Prunus species. The consensus map showing QTL hotspots will be accessible using various tools in GDR. Implications of the newly created resources and tools in GDR for breeding will be discussed.
Speakers
avatar for Michael Itam

Michael Itam

Clemson University
Co-authors
CH

Chun Huai Cheng

Washington State University
NA
DM

Dorrie Main

Washington State University
JY

Jing Yu

Washington State University
NA
JH

Jodi Humann

Washington State University
KB

Katheryn Buble

Washington State University
NA
KG

Ksenija Gasic

Clemson University
PZ

Ping Zheng

Washington State University
NA
SJ

Sook Jung

Washington State University
NA
TL

Taein Lee

Washington State University
NA
Wednesday September 25, 2024 1:10pm - 1:20pm HST
Coral 2

1:15pm HST

FLOR - Growth and Quality of Four Zinnia Cultivars Grown in Eastern South Dakota
Wednesday September 25, 2024 1:15pm - 1:25pm HST
Over the past two decades, interest in local specialty cut flower production in Eastern South Dakota has grown with several new flower farms being established across the region. However, there is currently no local, research-based information available to support growers in this new wave of interest. The purpose of this study was to assess the production and quality of four zinnia cultivars grown in Eastern South Dakota to support local specialty cut flower growers in their operations. This was a randomized complete block study with four zinnia cultivars (Zinderella Peach, Oklahoma Ivory, Queen Red Lime, and Benary’s Giant Purple) grown in 2022 and 2023. Zinnias were started in the greenhouse and then transplanted into the field in early summer each season. Harvest began each year when flowers reached maturity (July), with one harvest event taking place each week until the first frost. Harvested flowers were rated as marketable or non-marketable based on categories of stem length (18 -inch-long, 13-17.99-inch-long, 8-12.99-inch-long, and
Speakers
avatar for Kristine Lang

Kristine Lang

Assistant Professor and Extension Specialist, South Dakota State University
Co-authors
AB

Alexis Barnes

South Dakota State University
NA
EF

Ellen Fitzpatrick

South Dakota State University
NA
HV

Hannah Voye

South Dakota State University
Wednesday September 25, 2024 1:15pm - 1:25pm HST
Nautilus

1:15pm HST

LOCSY - The Missouri Produce Growers Video Newsletter – Digestible, Accessible and Impactful Extension Education
Wednesday September 25, 2024 1:15pm - 1:25pm HST
The Missouri Produce Growers Video Newsletter (MPGVN) was established in 2020, when the COVID-19 pandemic prevented in-person programming. The MPGVN is a bi-weekly video-based email newsletter targeting specialty crop farmers and is an offshoot of a project consisting of weekly live-streamed remote educational town halls, which lacked sufficient attendance to justify continuation. Maintaining impactful, ongoing digital engagement with extension audiences is challenging due to grower’s time constraints and competition with low time-investment and high reward non-extension educational media, such as video hosting and social media platforms. The 3–5-minute narrated PowerPoint™ presentations and live shot videos comprising the educational content in the MPGVN, are easily consumed, introducing key points on a timely cropping systems or farm management topic, with further topic resource links embedded. The MPVGN also features links to workshops, grants, and partner agency and non-profit resources. The core team of content contributors, 5 University of Missouri Extension Field Specialists in Horticulture, meets bi-weekly to plan content. Additional extension collaborators from various disciplines including agricultural business, natural resources, climatology, plant pathology, entomology, and food safety contribute content. The newsletter is created and distributed using the Dotdigital™ platform. The format and content have driven engagement amongst the 950 subscribers. The MPGVN engagement metrics relative to industry benchmarks for educational email newsletters are higher by 188% for unique opens, 345% for unique clickers, and 177% for click-to-open rate. To measure the impact of the MPGVN, a survey was sent to 948 subscribers in 2023, with a 14% response rate. Survey observations showed that 55% identified as growers “currently growing and selling produce or planning to grow and sell produce”, 45% as extension educators, and 5% as home gardeners. The survey showed that of the growers; 85% indicated knowledge gains; 78% reported adoption or change of farming practices ; 63% attended an extension class ; 56% connected with a partner agency ; 23% applied to grants ; and 10% received grant awards, thanks to information delivered in the newsletter. The MPGVN is a valuable educational tool, driving engagement with extension programming and resources.
Speakers
avatar for Justin Keay

Justin Keay

MU Extension
Justin Keay has served as a Field Specialist in Horticulture with University of Missouri Extension since 2018, and is currently based in Pike County, MO. He holds a bachelor’s degree in Environmental Studies from Washington University, and an M.S. in Integrated Agricultural Systems... Read More →
Wednesday September 25, 2024 1:15pm - 1:25pm HST
South Pacific 1

1:15pm HST

PB 2 - RNASeq Analysis Reveals Key Pathways Involved in Low Nitrogen Tolerance at the Onset of Storage Root Formation in Sweetpotato cv Bayou Belle
Wednesday September 25, 2024 1:15pm - 1:30pm HST
Nitrogen (N) is a key limiting macronutrient for crop growth and development and affects sweetpotato storage root formation and yield potential. In high-input production areas, excessive N application can suppress storage root formation and results in environmental pollution. The crop is also grown in low-input production systems with little or no N applications. In this study, sweetpotato cv Bayou Belle response to N deprivation during the establishment and storage root formation stages was investigated through a transcriptomic approach. RNA-seq data revealed a number of differentially expressed genes (DEGs) between N sufficient ( N) and N deficient (–N) conditions at 5, 10, and 15 days after planting (DAP). The number of significantly upregulated genes varied between timepoints. DEGs were further classified into functional categories and pathways to reveal putative functions. Gene Ontology annotation together with KEGG analysis revealed that majority of the DEGs are involved in sulfur compound metabolic process at 5 DAP and in ammonium transport for both 10 DAP and 15 DAP. These results provide valuable insights about the molecular mechanism of N regulation in sweetpotato adventitious roots undergoing storage root formation. These findings can lead to the development of tools and processes for improving N use efficiency and consistent storage root yields while reducing environmental impact in this globally important crop.
Speakers
avatar for Lisa Arce

Lisa Arce

Graduate Student, Louisiana State University
I am a PhD candidate focused on optimizing nutrient requirements for sweetpotatoes through advanced molecular techniques. My research utilizes expression data from qPCR assays and RNA sequencing to uncover genotype-specific responses to varying levels of nitrogen, phosphorus, and... Read More →
Co-authors
AV

Arthur Villordon

LSU Agcenter
CG

Cole Gregorie

LSU AgCenter
NA
DL

Don La Bonte

Louisiana State Univ
NA
MA

Mae Ann Bravo

Louisiana State University
MB

Marissa Barbosa

Lousiana State University
Wednesday September 25, 2024 1:15pm - 1:30pm HST
South Pacific 4

1:15pm HST

Writing Letters of Recommendation
Wednesday September 25, 2024 1:15pm - 1:45pm HST
People in academics and in the industry are regularly asked to write letters of recommendation for students, or even employees.  Writing recommendation letters is generally not something people learn or get formal guidance about.  And it’s not something that graduate students typically learn in their degree program. Undoubtedly, many members of our industry and profession will have (student) requests for a support letter for a scholarship, internship, employment, promotion, etc.  Based on personal experience reviewing hundreds of scholarships and award applications for several programs, this presentation will provide insight to assist in writing effective letters of recommendation. Providing a quality recommendation requires thought and effort to enhance the requestor’s success. Specific items a recommender should consider when crafting a recommendation ideally would include obtaining the person’s current resume; a copy of the scholarship or opportunity they are applying to so the nominator can specifically address and connect the individual to the opportunity; specific details including when the recommendation is needed, format required, etc.  Things to consider in the nomination letter include specific relationship to the individual; anecdotes with the particular person that can set the candidate apart; relevant industry/work experiences; awards, recognitions, and involvements, especially if they are not always included in the resume.  Authors should avoid letters that are too long, or too short, and adhere to any limitations outlined by the request. Avoid simply repeating or rehashing the student’s resume, instead provide more detailed insight and praise related to specific resume items.  Sometimes sharing the letter with the student can be appropriate.  It provides an opportunity for them to express concern for something that the author might have over-represented or, perhaps add something the author did not include that could increase impact.  Moreover, the letter is form of feedback and can foster a growth mindset and has the potential to enhance the student’s performance. Additionally, this activity should and can be documented for a faculty performance review.  Writing letters can be time consuming and rewarding, both for the nominee and yourself.  Hopefully insight shared will assist individuals with constructing quality support letters and recommendations.
Speakers
CM

Chad Miller

Assistant Professor; Department of Horticulture and Landscape, Colorado State University
Wednesday September 25, 2024 1:15pm - 1:45pm HST
Coral 5 - ASHS Hort Theater

1:20pm HST

CE - The Effect of Climate Change on Plants: A Case Study with Wheat
Wednesday September 25, 2024 1:20pm - 1:30pm HST
Anthropogenic climate change (ACC) will have considerable effects on plants, though the extent to which these effects are positive or negative has been controversial. For this poster, a fully factorial experiment combining water and temperature over broad ranges (10-90% soil water content under 16°C-40°C) was carried out to address three shortcomings that might help explain the contrasting effects of climate change on plants: testing only one climate variable (e.g., only water or only temperature), failure to account for nonlinear responses to climatic variables, and studying a limited number of response variables. The experiment utilized wheat as the model species and found that most dependent variables related to grain production showed the highest performance under 23-33°C and low water (
Speakers
KW

Kira Webster

PhD Candidate, University of Hawaii at Manoa
Wednesday September 25, 2024 1:20pm - 1:30pm HST
Coral 1

1:20pm HST

POM 2 - Taste Acidity and Other Important Fruit Characteristics of New Peach Varieties Compared with Concurrent Varieties During
Wednesday September 25, 2024 1:20pm - 1:30pm HST
Three new peach varieties and ‘Felicia’ ‘Evelynn’ and ‘Anna Rose’ were recently released from the Rutgers University tree fruit breeding program. These varieties are concurrent with some of the standard peach varieties. The comparison of taste acidity and other important fruit characteristics could help growers choose in case of planting these varieties or replacing the standard varieties during the same harvest window. Fruit characteristics such as flesh color, crop load, shape, skin color, fuzz, attractiveness, stone characteristics (free, semi-free, or cling), flesh color, bacterial spot, on fruits and leaves, taste acidity, juice total soluble solids (TSS) concentration (°Brix), and total titratable acidity (TTA) (%) were measured. Here we compare these varieties to highlight the differences and challenges amongst these varieties on their path to full consumer appreciation. Results indicate that average TSS and firmness were higher in ‘Felicia’ (12.9 brix; 12.4 lb) and ‘Evelynn’ (11.9 brix; 10.9 lbs.) compared to ‘Redhaven’ (11.1 brix; 8.14 lbs.). Also; average TSS and firmness were higher in ‘Anna Rose’ (14.4 brix; 13.68 lbs.) compared to ‘Klondike’ (12.0 brix; 11.3 lbs.) and ‘White Lady’ (10.9 brix; 9.64 lbs.). In summary, new yellow-fleshed peach varieties ‘Felicia’ and ‘Evelynn’ have great potential to replace the old standard variety, ‘Redhaven’. Similarly, the new white-fleshed peach variety ‘Anna Rose’ has great potential to replace old standard varieties ‘White Lady’ and ‘Klondike’.
Speakers
HG

Hemant Gohil

Extension AgentII/Associate Professor, Rutgers Cooperative Extension
Co-authors
DW

Daniel Ward

Rutgers University
NA
Wednesday September 25, 2024 1:20pm - 1:30pm HST
Coral 2

1:25pm HST

FLOR - Effects of Photoperiod Treatments on Stock Plants and Cutting Rooting of Callirhoe involucrata
Wednesday September 25, 2024 1:25pm - 1:35pm HST
Effects of Photoperiod Treatments on Stock Plants and Cutting Rooting of Callirhoe involucrata James E. Klett and Ronda Koski Colorado State University Department of Horticulture and LA Production of attractive and desirable plants, native to the plains and prairies of central North America, can sometimes be limited due to problems associated with successful rooting of vegetative cuttings. Species that have a long period of bloom can be challenging to vegetatively propagate during the growing season, because sufficient juvenile (vegetatively) growth is necessary for high-quality cuttings. Callirhoe involucrata (winecups) is a native herbaceous perennial with sprawling stems, palmately divided leaves, and dark pink flowers that creates a long lasting, colorful groundcover in landscapes with limited or no supplemental irrigation. Winecups can be difficult to propagate due to seed dormancy and poor rooting of vegetative cuttings. Manipulating the growing conditions of stock plants to suppress reproductive growth may improve rooting success of vegetative cuttings. Based on previous research at Colorado State University with other ornamental perennials and programmed photoperiods, winecups were grown under three programmed photoperiods to determine if a particular photoperiod could be used to suppress reproductive growth and promote vegetative growth. The research was replicated three times, all conducted in a similar fashion, but at different times of the year. Results indicate that plants grown under 10-hour light and 8-hour light photoperiods remained vegetative longer when compared to plants grown under 12-hour photoperiod. Plants grown under 12-hour photoperiods had greater mean ending dry weight when compared to plants grown under 10 hour and 8-hour photoperiods. Cuttings harvested from plants grown under 8-hour photoperiod had higher percent rooting and greater number of roots per cutting when compared to vegetative cuttings harvested from plants grown under 12-hour photoperiod. Based on these research findings, plant propagators may be able to increase production of winecups by growing stock plants under 8-hour photoperiod.
Speakers
JK

James Klett

Colorado State University
Wednesday September 25, 2024 1:25pm - 1:35pm HST
Nautilus

1:25pm HST

LOCSY - The Center for Sustainability of Farms and Families: Impacts of The Small Scale Farm Grant on Local Foods in Kentucky.
Wednesday September 25, 2024 1:25pm - 1:35pm HST
The Center for Sustainability of Farms and Families: Impacts of The Small Scale Farm Grant on Local Foods in Kentucky. JONI NELSON*, MATTHEW BEHRENDS, ALLISON NOEL, and KIRK W. POMPER. College of Agriculture, Health, and Natural Resources Cooperative Extension Program, Kentucky State University, Frankfort, KY 40601 Kentucky State University’s Small Farm Grant Program managed through the Center for Sustainability of Farms and Families supports new economic opportunities for limited resource farmers with funding from the Kentucky Agricultural Development Board and the USDA-NIFA capacity building grant “Extension Approaches to Support Socially Disadvantaged Farmers and Producers”. The Small Farm Grant Program, which began in 2012, has awarded more than $8.5 million through 1,976 applications to farmers from 114 Kentucky counties. This funding has been distributed through mini-grants for categories including Value-Added, Organic, Aquaculture, Food Insecure, and Agroforestry projects. New categories added in 2024 including Food Systems Resiliency and Emerging Agricultural Technologies have been added in addition to a beginning beekeeper program in our ongoing efforts to address the diverse needs of Kentucky producers. These new categories support the efforts of producers to implement innovative practices or technologies that enhance sustainability and resource efficiency on the farm. Program funding supports a wide range of needs such as mobile freezer units, pond aerators, canning equipment for fruits and vegetables, tractor implements to support organic crop integrity, goat cheese and soap making equipment, root cellars, equipment for food banks and farmers markets, agroforestry practices, and numerous others. This program has also assisted many new farmers with entering local markets and education toward production methods as well as aiding their efforts to support local economies and cooperative networks.
Speakers
JN

Joni Nelson

Manager CSFF, Kentucky State University
Co-authors
KP

Kirk Pomper

Kentucky State University
Dr. Kirk W. Pomper is the Professor of Horticulture in the College of Agriculture, Community, and the Sciences at Kentucky State University in Frankfort, Kentucky. As Horticulture Research Leader, his program is focused on research and Extension efforts toward developing pawpaw as... Read More →
MB

Matthew Behrends

Kentucky State University
Wednesday September 25, 2024 1:25pm - 1:35pm HST
South Pacific 1

1:29pm HST

Vegetable Crops Management 3 (VCM 3)
Wednesday September 25, 2024 1:29pm - 3:00pm HST
Monitoring High Tunnel Soil Temperature Fluxes to Understand Soil Health Implications - Bret Lang
Performance of Semi-Controlled Protective Net House at Farmers’ Field to Make The Year-Round Availability of Quality Vegetables and Higher Benefit Cost - AKM Quamruzzaman
Evaluating The Effect Of Cultivar On Garlic Growth And Yield - Stephen Mensah
Optimizing Butterhead Lettuce Production in a High Tunnel Environment - Alan Walters
Resource Use Efficiency, Yield, and Quality of Amaranth Grown as a Summer Leafy Green in Alternative Soilless Systems - Pradip Poudel
Differential performance of Radicchio cultivars in New Hampshire - Becky Sideman
Comparative Analysis of Biodegradable Mulch Use in Hill-side Strawberry Production - Amrita Mukherjee

Moderator
avatar for Stephen Mensah

Stephen Mensah

Graduate Research Assistant, North Dakota State University
Greetings! I'm Stephen Mensah, proud son of Ghana. My journey began with a Bachelor's degree in Agriculture from the esteemed University of Cape Coast, Ghana. Now, I'm embarking on an exciting chapter as a graduate student at North Dakota State University, passionately pursuing a... Read More →
Wednesday September 25, 2024 1:29pm - 3:00pm HST
Kahili

1:30pm HST

POM 2 - Role of Silicon in Peach Water Stress Tolerance
Wednesday September 25, 2024 1:30pm - 1:40pm HST
Water stress in young fruit trees can reduce tree growth and impair future crop productivity. Silicon (Si) has shown a positive effect in mechanisms related to water relations, specifically water uptake and transport, as well as physiological and anatomical changes in leaves of annual plants. These findings suggest that Si could induce adaptive changes that could help trees cope with water deficit. The objective of this experiment was to evaluate the role of Si on water status, sap flow, and gas exchange of young peach trees under water deficit. Two-year-old ‘Julyprince’ peach trees grown under controlled conditions received either 100% (well-watered trees) or 50% (stressed trees) of their crop evapotranspiration needs three times a week. Once trees in the two treatments displayed a difference in water status, Si was applied weekly by foliar spray (0 or 20 mg L-1). The combination of these two factors resulted into a completely randomized factorial design with four treatments. Results showed an interaction between irrigation regime and Si application. Si increased sap flow in well-watered trees and reduced that of stressed trees, although this effect was more significant the day after irrigation. Furthermore, Si significantly improved the water status of trees under water deficit but did not influence that of well-watered trees. These results seem to indicate that the role of Si in improving tolerance to water deficit is temporary and dependent on the tree water status.
Speakers
avatar for Noah Willsea

Noah Willsea

Clemson University
Co-authors
IM

Inma Martos-Garcia

University of Cordoba
NA
JC

Juan Carlos Melgar

Clemson University
NA
MB

Maria Benlloch-Gonzales

University of Cordoba
NA
RF

Ricardo Fernandez-Escobar

University of Cordoba
NA
Wednesday September 25, 2024 1:30pm - 1:40pm HST
Coral 2

1:30pm HST

PB 2 - Introducing CHiDO – a No Code Genomic Prediction Software implementation for the Characterization & Integration of Driven Omics
Wednesday September 25, 2024 1:30pm - 1:45pm HST
Climate change represents a significant challenge to global food security by altering environmental conditions critical to crop growth. Plant breeders can play a key role in mitigating these challenges by developing more resilient crop varieties; however, these efforts require significant investments in resources and time. In response, it is imperative to use current technologies that assimilate large biological and environmental datasets into predictive models to accelerate the research, development, and release of new improved varieties. Leveraging large and diverse data sets can improve the characterization of phenotypic responses due to environmental stimuli and genomic pulses. A better characterization of these signals holds the potential to enhance our ability to predict trait performance under changes in weather and/or soil conditions with high precision. This presentation introduces CHiDO, an easy-to-use, no-code platform designed to integrate diverse omics datasets and effectively model their interactions. With its flexibility to integrate and process data sets, CHiDO's intuitive interface allows users to explore historical data, formulate hypotheses, and optimize data collection strategies for future scenarios. The platform's mission emphasizes global accessibility, democratizing statistical solutions for situations where professional ability in data processing and data analysis is not available.
Speakers
DJ

Diego Jarquin

University of Florida
Co-authors
FG

Francisco Gonzalez

University of Florida
NA
JG

Julian Garcia-Abadillo

University of Florida
NA
Wednesday September 25, 2024 1:30pm - 1:45pm HST
South Pacific 4

1:30pm HST

VCM 3 - Monitoring High Tunnel Soil Temperature Fluxes to Understand Soil Health Implications
Wednesday September 25, 2024 1:30pm - 1:45pm HST
High tunnel soil health is crucial for successful and sustainable crop production within protected environments. Soil microbial activity is highly temperature-dependent, and soils that are slightly warmer will foster increased metabolic rates within soil communities enhancing microbial diversity and enzymatic activity, promoting nutrient availability. However, little is understood about the potential for microbial activity during colder seasons in norther latitudes when high tunnels are taken out of production and soils are left fallow. Temperature variation in high tunnels could also create variation in microbial community activity, creating spatial nutrient variation with impacts on production the following season. To analyze soil temperature fluxes, we buried an array of 27 soil sensors four inches deep within the soil in a newly built, 30-foot-wideby 96-foot-long tunnel located in Brookings, South Dakota. The high tunnel was oriented east to west and soil was bare. Soil temperatures were recorded at 30-minute intervals from December 22 to March 15, (2023 – 2024). Air temperature and light (lux) data was also collected inside of the high tunnel as well as external weather data from a nearby (>1km) Mesonet weather station. We used multiple linear regression to model the relationship between average internal soil temperature and internal light and temperature data. We also compared sensor location (latitude, longitude, and Euclidean distance from the center of the high tunnel) on soil temperature within the high tunnel using an ANOVA and multiple linear regression to examine how sensor location was related to soil temperature. Our top model of internal soil temperature showed light, internal temperature, and the interaction between light and internal temperature explained a large amount of high tunnel soil temperature variation (R2 = 0.87, p < 0.0001). There was also significant variation in soil temperature throughout the high tunnel, with the daily mean difference of 3.12 degrees Celsius (p < 0.0001) observed between our sensor at the center of the high tunnel and our sensor near the northwest corner of the high tunnel. Our top model showed that latitude, the quadratic of longitude, and the Euclidean distance from the center of the high tunnel explained a moderate amount of high tunnel soil temperature variation (R2 = 0.44, p < 0.0001). This analysis demonstrates a need to further investigate how microbial communities react to temperature variation within high tunnels when they are not in production.
Speakers
BL

Bret Lang

Student, South Dakota State University
Co-authors
KL

Kristine Lang

South Dakota State University
LP

Lora Perkins

South Dakota State University
NA
Wednesday September 25, 2024 1:30pm - 1:45pm HST
Kahili

1:30pm HST

HortLegends Interviews
Wednesday September 25, 2024 1:30pm - 3:30pm HST
Wednesday September 25, 2024 1:30pm - 3:30pm HST
Sea Pearl 4

1:35pm HST

FLOR - Consumer testing and surveys indicate a market for Peperomia mauiensis, an endemic succulent with potential use as an indoor potted plant
Wednesday September 25, 2024 1:35pm - 1:45pm HST
Peperomia mauiensis is an endemic succulent found on the islands of Maui, Molokai and Lanai. Its reddish stems and foliage as well as its small stature make it a potential indoor ornamental plant. To assess consumer acceptance, a public plant giveaway with surveys was conducted. Fifty-six plants in plastic and ceramic pots were distributed to the public with the completion of a survey. A six-month follow up survey was conducted to assess the condition of the plant and obtain consumer feedback. During the plant giveaway, 77% of participants completed the survey. Nearly all respondents are willing to buy the plant and recommend it to friends and co-workers. Thirty percent of respondents are willing to buy the plant for $5 in a ceramic pot and $3 to $5 for a plastic pot. Six months after the giveaway, 39.5% of initial respondents completed the follow-up survey. About half of the respondents (52.9%) reported the plant was growing healthy and vigorous. The majority of the respondents did not fertilize the plant (81.3%) and did not report any pest (94.1%) and disease (88.2%) issues. Most responded that they liked it as a houseplant (88.2%) and it was easy to maintain (82.4%). Most respondents (35.3%) were willing to buy the plant again for $5. Results of the surveys indicate that there is consumer interest in Peperomia mauiensis as a new native indoor plant.
Speakers
OB

Orville Baldos

Associate Researcher, University of Hawaii at Manoa
Co-authors
Wednesday September 25, 2024 1:35pm - 1:45pm HST
Nautilus

1:35pm HST

LOCSY - The Waimānalo Learning Center: Land-Grant Experiment Stations as a Space to Heighten Community Engagement and Advanceme
Wednesday September 25, 2024 1:35pm - 1:45pm HST
The Waimānalo Learning Center (WLC) is a University of Hawai’i (UH) project established in 2012 through seed funding from the UH College of Tropical Agriculture and Human Resources (CTAHR) and the Harold K.L. Castle Foundation. Located at CTAHR's Waimānalo Research Station, the WLC aims to transform the station into a community learning center that promotes food security, self-sufficiency, and healthy eating, particularly for indigenous and underserved populations. The UH physical facilities supporting WLC include an aquaponics facility and outdoor classroom adjacent to certified organic research plots. A Community Coordinator was added in 2014, and since then the WLC has rapidly expanded its programming through close partnerships with local schools and community organizations. In 2017 the WLC has partnered with a local non-profit, Ke Kula Nui O Waimānalo to run food sovereignty programs including aquaponics (MALAMA), agroforestry (Ulupono Mahi ‘Āina), and backyard egg production (Hui Hua Moa). In a survey of key partners, the vast majority of 75 respondents strongly agreed that: 1) The WLC has been a valuable partner for their organization (98.6%); 2) The WLC plays an important role in serving the Waimanalo community (95.9%); 3) The WLC has helped them elevate their group’s mission and vision (94.5%). Echoing the comments of others, one respondent stated “The Waimānalo Learning Center has served as important ʻāina (land) for our haumana (students) to build pilina (relationship), hana (work), and take on greater kuleana (responsibility) with. The staff and facilities have allowed us to grow and deepen our curriculum of place-based, ʻāina work within our community.” The results from 12 years of activity suggests that the WLC is an effective model for using Land Grant Research Stations to address community needs around food, health and cultural preservation, and increase community engagement and advancement.
Speakers
HI

Heidi Ilima Ho-Lastimosa

University of Hawai'i at Manoa, Ke Kula Nui O Waimanalo
NA
Co-authors
IR

Ikaika Rogerson

Ke Kula Nui O Waimanalo
NA
JC

Jane Chung-Do

University of Hawai'i at Manoa, Ke Kula Nui O Waimanalo
NA
JS

Jari Sugano

University of Hawaii at Manoa
NA
KH

Kenneth Ho

Ke Kula Nui O Waimanalo
NA
KD

Kirk Deitschman

Ke Kula Nui O Waimanalo
NA
LK

LeShay Keliiholokai

Ke Kula Nui O Waimanalo
NA
RF

Richard Fisher

University of Hawai'i at Manoa
NA
TR

Theodore Radovich

University of Hawai'i at Manoa, Ke Kula Nui O Waimanalo
NA
Wednesday September 25, 2024 1:35pm - 1:45pm HST
South Pacific 1

1:40pm HST

POM 2 - Mechanization Reduces Pruning and Harvest Labor Time in the Cider Apple Orchard
Wednesday September 25, 2024 1:40pm - 1:50pm HST
While fresh-eating, dessert apples dominate the apple market of Washington State, the over 30-fold national increase in volumetric cider production over the past 15 years indicates there is growing demand for specialty cider apples, or apple cultivars with positive cider-making attributes such as high polyphenol content. In a commercial cider orchard, the labor costs associated with thinning, pruning, and harvest comprise a majority of the annual variable production costs once the orchard is in full production; thus, reducing the labor time to perform these tasks has the potential to increase the appeal and profitability of growing cider apples. Further, when cider apples are pressed into juice soon after harvest, cosmetic damage and bruising of the fruit during harvest has minimal impact on juice quality, indicating a strong potential for harvest mechanization. This study separately examines mechanical hedging and mechanized over-the-row harvest (using a modified Oxbo-Korvan 930 harvester) in a multi-cultivar cider apple orchard. Mechanical hedging both sides of the orchard row occurred at a rate of 2.1-3.5 seconds per meter across 5 years. Across 16 cultivars and 3 years, mechanical harvest occurred at a rate of 2.7-3.1 seconds per meter, and the average capture efficiency was 82% with no significant differences between cultivars. Based on these results, a combination of mechanical pruning and harvest could significantly lower labor time, and thus the total production costs, associated with growing cider apples.
Speakers
CM

Carol Miles

Washington State University
Co-authors
AK

Aidan Kendall

Washington State University
NA
ES

Ed Scheenstra

Washington State University
NA
SB

Seth Brawner

Washington State University
Wednesday September 25, 2024 1:40pm - 1:50pm HST
Coral 2

1:44pm HST

Ecological Physiology/Horticultural Plant Reproductive Biology/Plant Growth Regulation (EcoPhys/HPRB/PGR)
Wednesday September 25, 2024 1:44pm - 2:35pm HST
Multiple Modeling Approaches Reveal Temperature Dependent Germination Traits of Vegetable Varieties - Miro Stuke
Identifying Pollinators Present on Flowers of the Pawpaw Cultivars 'Sunflower' and 'Susquehanna' - Subas Thapa Magar
Molecular Assessment of Heat Sensitivity in Broccoli Flowering - Thomas Bjorkman
Morpho-physiological Response of Plectranthus amboinicus under Flooding and Drought Stress - Samuel Asiedu
Effects of Paclobutrazol, Progressive-raising Temperature and Spike-truncated Treatments on Phalaenopsis Join Grace ‘TH288-4’ - Yi Chien Lu

Moderator
ST

Subas Thapa Magar

Kentucky State University
A major change happened when the tragedy of Corona hit the world. Hi, I am Subas Thapa Magar, a graduate research assistant under the supervisor Dr. Kirk Pomper. I am currently working on Pawpaw (Asimina Triloba), the native fruit tree of the United States of America, and my research... Read More →
Wednesday September 25, 2024 1:44pm - 2:35pm HST
South Pacific 3

1:45pm HST

EcoPhys/HPRB/PGR - Multiple Modeling Approaches Reveal Temperature Dependent Germination Traits of Vegetable Varieties
Wednesday September 25, 2024 1:45pm - 1:55pm HST
Crop seed germination is a critical factor in food production. Germination traits vary between different vegetables and between cultivars of the same vegetable. Traits such as germination proportion and the rate and uniformity of germination also contribute to the success of vegetable cultivars to regional temperature averages and regimes. Temperature is a major abiotic factor in seed germination, and selection of seed varieties suited for local temperatures is important to successful crop establishment. Here we aim to model the germination traits of several Korean and North American vegetable cultivars in relationship to temperature. We hypothesize that a model based on time and temperature will represent germination traits across multiple cultivars and species of vegetable. Additionally, we hypothesize that predicted germination traits will be similar within species but will vary between cultivars of the same species based on the regional temperature norms. Eleven vegetable cultivars including two corn, four radish, two pepper, and three onion, were germinated in growth chambers set at 5°C intervals between 5 and 40°C with a 12 hour photoperiod. Germination, defined by radicle emergence equal to the length of the seed, was monitored and recorded daily. Two modeling approaches were used. 1) A time to event model using the drcSeedGerm package in R was used to determine maximum germination proportion (Pmax), germination rate at 50% germination (GR50), and uniformity. 2) A 12-parameter compartmental temperature and time model was implemented, parameterized, and validated in the Cropbox modeling framework. Results show differing temperature responses in germination traits. Additionally, high uniformity was observed in most varieties within the optimal temperature range. The optimal temperature for germination was broad for the vegetable cultivars tested here, with a rapid decrease in Pmax at high and low temperature extremes, except in onions, which showed high Pmax even at 5°C, and a gradual decline above 25°C. All cultivars showed decreased Pmax at 40°C. A time to event model was able to predict germination traits in several crop species. The compartmental model was better equipped to handle heat induced seed degradation but was less parsimonious for determining germination probability and germination rate related parameters. Modeling crop germination traits can provide important context for selecting appropriate cultivars for local climates. A thermal time to event model and a compartmental model both provide potential frameworks for modeling germination traits of diverse vegetable species.
Speakers
MS

Miro Stuke

University of Washington
Co-authors
AH

Arthur Hsin-Wu Hsu

University of Idaho
NA
KY

Kyungdahm Yun

Jeonbuk National University
NA
SZ

Sabrina Zerrade

University of Washington
NA
SK

Soohyung Kim

University of Washington
Wednesday September 25, 2024 1:45pm - 1:55pm HST
South Pacific 3

1:45pm HST

FLOR - Trials of Low Elevation Tolerant Leucospermum Hybrids on Oahu, HI
Wednesday September 25, 2024 1:45pm - 1:55pm HST
Leucospermum species are temperate climate plants native to South Africa and have restricted production regions in Hawaii limited to elevations of 457 m or higher. This limitation and competition for land has been partially responsible to a decline in Leucospermum production in Hawaii. Agriculture land at lower elevations is more abundant and cheaper, and is an option for growing low elevation tolerant varieties. Breeding programs at the University of Hawaii at Manoa have focused on developing hybrids that will perform to commercial standards for plant growth, yield and quality, and grow at or near sea level. 14 hybrids of Leucospermum were trialed at 152 m elevation on Oahu HI. 10 hybrids exhibited adequate survival and were selected for continued trial. Weahter, plant growth (height and width), flower count, flowering seasonality, and plant health data were collected from 2021-2023. Flower length and quality data, and vase life data were collected in 2024. Average relative humidity was 79%, average monthly rainfall was 3.53 cm, and average temperature was 21.8 C with maximum temperature of 33.4 C and low of 14.2 C. The 10 hybrids all had acceptable fitness at 500’ elevation. Some hybrids showed more fitness than others. Hybrid 602 had the highest overall average health (3.8) and highest total flower count. Hybrids 620 and 626 also showed excellent fitness as well as highest flower counts. While none of the hybrids are considered unfit for production in similar conditions, some did perform less well. Hybrid 88 and Hybrid 633 had the lowest average health at 3.0 followed closely by Hybrids 583 (3.2), 679 (3.3), and 563 (3.3). These varieties also had some of the lowest total flower counts. All of the hybrids in this study are considered potential candidates for release and continued trialing. Future work can include long term trialing of these plants, and trialing at different microclimates across the State.
Speakers
RG

Russell Galanti

Extension Educator, University of Hawaiʻi
Co-authors
AR

Alberto Ricordi

University of Hawaii at Manoa
KL

Kenneth Leonhardt

University of Hawaii at Manoa
NA
Wednesday September 25, 2024 1:45pm - 1:55pm HST
Nautilus

1:45pm HST

PB 2 - Tissue Culture-Free Genome Editing in Plants Using RNA Viruses
Wednesday September 25, 2024 1:45pm - 2:00pm HST
Genome editing represents a pivotal tool for advancing biological discovery and crop enhancement. However, its widespread application in crop improvement has been hindered by inefficient delivery methods and the dependence on tissue culture for generating gene-edited plants. RNA viral vectors present a promising alternative for delivering gene-editing reagents while circumventing the need for tissue culture. I have developed methods for: (1) Achieving multiplexed, heritable gene editing in tomato through viral delivery of isopentenyl transferase and single-guide RNAs to latent axillary meristematic cells; (2) Inducing heritable, multinucleotide deletions in Nicotiana benthamiana using viral delivery of a repair exonuclease and guide RNAs; and (3) Enabling heritable base editing in Arabidopsis via RNA viral vectors. These approaches are likely transferable to diverse plant species, paving the way for scalable gene editing in agriculture.
Speakers
DL

Degao Liu

Texas Tech
Wednesday September 25, 2024 1:45pm - 2:00pm HST
South Pacific 4

1:45pm HST

VCM 3 - Evaluating The Effect Of Cultivar On Garlic Growth And Yield
Wednesday September 25, 2024 1:45pm - 2:00pm HST
Assessing the impact of cultivar on garlic growth and yield ensures satisfactory productivity and better management of genetic resources for growers in North Dakota. A field experiment was conducted at the NDSU Horticulture Research Farm, located near Absaraka, ND to evaluate the effect of cultivar on the growth and yield of garlic. Twenty-nine garlic cultivars were arranged in a randomized complete block design (RCBD) with 4 replications. The leaf number, bulb diameter, bulb weight, scape weight and length were recorded throughout the growing season. The results showed significant differences between cultivars for all the variables evaluated. The highest bulb diameter(60.5 cm) was recorded in ‘German White’, while the lowest diameter (38.9 cm) was recorded in ‘German Red’. The highest bulb weight(73.4 g) was with ‘German White’ while ‘Italian Loiacono’ resulted in the lowest bulb weight(21.2 g).The number of leaves was recorded at 30, 45, 60, and 80 days after sprouting (DAS). The average number of leaves was similar for all cultivars until 60 DAS when ‘Ukrainian Red’ had the highest number of leaves (11) and ‘German Red’ had the fewest leaves (7). ‘Dakota White’ produced the longest scapes while ‘Italian Loiacono’ and ‘White Spring’ did not produce any scapes. ‘German White’ recorded the heaviest scapes (13.8 g). In conclusion garlic cultivar affected the growth and yield when grown under North Dakota environmental conditions. ‘German White’ appeared to be the most adapted to North Dakota environmental conditions while ‘Italian Loiacono’ and ‘German Red’ were the least adapted.
Speakers
avatar for Stephen Mensah

Stephen Mensah

Graduate Research Assistant, North Dakota State University
Greetings! I'm Stephen Mensah, proud son of Ghana. My journey began with a Bachelor's degree in Agriculture from the esteemed University of Cape Coast, Ghana. Now, I'm embarking on an exciting chapter as a graduate student at North Dakota State University, passionately pursuing a... Read More →
Co-authors
Wednesday September 25, 2024 1:45pm - 2:00pm HST
Kahili

1:50pm HST

Tropical Plant and Soil Science, University of Hawai'i at Manoa
Wednesday September 25, 2024 1:50pm - 2:20pm HST
Noa Lincoln will present - Indigenous crops and cropping systems have tremendous potential to improve markets, livelihoods, human health, cultural identity, ecological outcomes, and adaptation to climate change. With one example--breadfruit--I will showcase how work within the TPSS department has supported tremendous growth in the industry to realize numerous positive impacts on Hawai‘i's food system.

Rosemary Gutierrez-Coarite will present - Maui Edible Crops Program: Integrated pest management of the most important pest of edible crops in Maui County, conducting field and lab trials. Soil fertility and water stress management field trials for improving crop productivity, reducing pollution, and increasing the resilience of farming systems.

Alberto Ricordi will present as an CTAHR alumni and share an overview of recent extension activities (workshops, events, trails, etc.). Students will be able to see that there is an opportunity to engage in CTAHR Extension activities during their studies to get prepared for extension as a career, make connections in the industry, and prepare for the marketplace.
Speakers
AR

Alberto Ricordi

University of Hawaii at Manoa
NL

Noa Lincoln

Associate Professor - Indigenous Crops, University of Hawaii at Manoa
Noa Kekuewa Lincoln is an Associate Professor with a focus on Indigenous Crops and Cropping Systems, in the College of Tropical Agriculture and Human Resources at the University of Hawai'i at Mānoa, and the PI of the Indigenous Cropping Systems Laboratory.  He is the President and... Read More →
RG

Rosemary Gutierrez Coarite

University of Hawaii at Manoa
KD

Kent D. Kobayashi

Interim Dept. Chair, TPSS Dept., Univ. of Hawaii at Manoa
Wednesday September 25, 2024 1:50pm - 2:20pm HST
Coral 5 - ASHS Hort Theater

1:55pm HST

EcoPhys/HPRB/PGR - Identifying Pollinators Present on Flowers of the Pawpaw Cultivars 'Sunflower' and 'Susquehanna'
Wednesday September 25, 2024 1:55pm - 2:05pm HST
Pawpaw (Asimina Triloba) is a temperate species of tropical Annonaceae plant family native to the United States. They are small to medium-sized deciduous trees that bear unique and flavorful fruit. Pawpaw fruits are used in various culinary products such as jam, bread, ice cream, cookies, and even to produce brandy. Additionally, they contain antioxidants and acetogenins, which are currently being researched for their potential in cancer treatment. Fruit sets may be limited in some cultivars due to pawpaw flowers being protogynous and self-incompatible, blooming at various stages of development from late March to April. Consequently, the role of insects in pollination is crucial for increasing fruit production. To address this gap in research, this study aims to identify the different insect orders that visit both flowering and non-flowering branches of two distinct pawpaw cultivars (Sunflower and Susquehanna), along with their respective abundances. Ten trees from each pawpaw cultivar were selected to investigate the various pollinator types. In a completely randomized design, 40 wire cage traps with tangle trap adhesive were placed on both the flowering and non-flowering branches of each chosen cultivar. After 18 days of the flowering period, the traps were collected, and the insects captured were identified by order and counted. The data were analyzed using R software (R 4.3.2) and subjected to a one-way ANOVA with flower and non-flower of each cultivar, a two-way ANOVA, Least Significant Difference (LSD) means separation, with flowers and cultivars as the treatments. In 2023, ‘Sunflower’ had significantly more Dipterans and total insects (p-value: 0.3373 NS and 0.3740 NS). In comparison, ‘Susquehanna’ had significantly more Coleopterans (p-value: 0.40525 NS), and in ‘Susquehanna’, coleopterans had significantly more in the flower-branch (p-value: 0.0255*) for 2023. Additionally, data for 2024 will also be reported and will include additional data on insects observed inside the female receptive and matured male flowers from each cultivar.
Speakers
ST

Subas Thapa Magar

Kentucky State University
A major change happened when the tragedy of Corona hit the world. Hi, I am Subas Thapa Magar, a graduate research assistant under the supervisor Dr. Kirk Pomper. I am currently working on Pawpaw (Asimina Triloba), the native fruit tree of the United States of America, and my research... Read More →
Co-authors
JL

Jeremy Lowe

Kentucky State University
KP

Kirk Pomper

Kentucky State University
Dr. Kirk W. Pomper is the Professor of Horticulture in the College of Agriculture, Community, and the Sciences at Kentucky State University in Frankfort, Kentucky. As Horticulture Research Leader, his program is focused on research and Extension efforts toward developing pawpaw as... Read More →
SC

Sheri Crabtree

Kentucky State University
Wednesday September 25, 2024 1:55pm - 2:05pm HST
South Pacific 3

1:59pm HST

Hemp Research and Extension 2 (HEMP 2)
Wednesday September 25, 2024 1:59pm - 3:30pm HST
In-vitro Screening of Native Plant Crude Extracts Against Major Plant Pathogens Affecting Cannabis and Specialty Food Crops of Louisiana - Jennifer Blanchard
Investigating Fiber Hemp Seed Size Impact on Germination, Emergence, and Early Growth Rate - Samantha Carroll
Modified Media and Lighting for Repeated In Vitro Cutting Cycles of Cannabis Sativa - Molly McKay
QTL mapping and gene discovery for seed traits in hemp (Cannabis sativa L.) F2 mapping populations - Luis Monserrate
Dynamics of Cannabinoid Accumulation and Morphological Changes in Cannabis Inflorescences - Samuel des Bordes
Variable Planting Date Influences on Growth and Development of Floral Hemp in North Dakota - Brock Schulz

Moderator
SD

Samuel des Bordes

Ph.D. Candidate, Louisiana State University
Wednesday September 25, 2024 1:59pm - 3:30pm HST
South Pacific 2

1:59pm HST

Growth Chambers and Controlled Environments 3 (CE 3)
Wednesday September 25, 2024 1:59pm - 3:45pm HST
Effects of Light Sources and Fertilizers on Biomass Production of Nine Lettuce Cultivars in a Hydroponic Nutrient Film Technique System - Dario Rueda Kunz
Enhancing Lettuce Yield: Strategies for Fertility Management in Soilless Growth Systems. - Bryce Waugh
Effect of ZnO and SiO2 Nanoparticles on Growth and Physiology of Hydroponic Lettuce Under Salinity Stress - Chungkeun Lee
An Intermediate Calcium-mobilizing Biostimulant Concentration Controls Tipburn of Two Greenhouse Hydroponic Lettuce Cultivars Without Affecting Growth -Shem Msabila
Optimizing Sampling Methods for Sap Extraction to Enhance Plant Nutrient Analysis in CEA - Husnain Rauf
Effects of Potassium and Iron Supplements and Late Nitrogen Restriction on Aquaponic Taro (Colocasia esculenta) Corm Production - Andrew Bohringer
Advantages of a novel in situ pH measurement for soilless media - Hikari Skabelund

Speakers
DR

Dario Rueda Kunz

Texas Tech University
Wednesday September 25, 2024 1:59pm - 3:45pm HST
Coral 1

1:59pm HST

Propagation (PROP)
Wednesday September 25, 2024 1:59pm - 3:45pm HST
Enhancing Germination of Silver Saw Palmetto (Serenoa repens form glauca): Water Uptake, Temperature, and Pre Sowing Treatment Effects - Vania Pereira
Propagation Strategies and Epigenetic Variations in Vaccinium Berry Crops - Samir Debnath
The Application Timing of a Cytokinin B-Mo-based Product Affects the Growth and Non-Structural Carbohydrates Contents of Cuttings During Adventitious Root Development - Mayra Toro Herrera
Characterizing the effects of PPFD and VPD during indoor acclimation of Chrysanthemum cuttings - Ana Sofia Gomez
Asexual Propagation of Salix humilis Using Dormant Hardwood Cuttings After Stock Plant Coppicing and Fungicide Application - Hazel Schrader
Evaluation of Auxin Application and the Presence or Absence of Roots or Inflorescences in the Propagation of Variegated Carex wahuensis subsp. wahuensis - Paulo Matos
Evaluating the Effect of Plant Growth Hormone Types and Rates on Osmanthus spp. Propagation - Yulong Chen
Evaluating Leaf Cutting Success for Achimenes Propagation - Chad T. Miller

Moderator
PM

Paulo Matos

Teaching Assistant, University of Hawai'i
Wednesday September 25, 2024 1:59pm - 3:45pm HST
Lehua Suite

2:00pm HST

CE 3 - Effects of Light Sources and Fertilizers on Biomass Production of Nine Lettuce Cultivars in a Hydroponic Nutrient Film Technique System
Wednesday September 25, 2024 2:00pm - 2:15pm HST
This study investigated the impact of two light sources and fertilizers on the growth of nine lettuce cultivars in a hydroponic nutrient film technique system. The research was prompted by reduced plant growth and acidity issues observed in the nutrient solutions in which Lettuce (Lactuca sativa cv. Salanova) was growing in previous experiments. The hypothesis posited that adjusting the ammonium/nitrate ratio in the nitrogen fertilizer source could mitigate acidity drops in the nutrient solution and consequently enhance biomass production. This experiment was conducted at Texas Tech University's Horticulture Gardens and Greenhouse Complex from October 10 to November 22, 2023. Treatments included combinations of two light sources (WhiteLEDs and LumiGrow TopLight Node™) and two fertilizer brands (Oasis and MaxiGro) containing different ratios of ammonium:nitrate; Oasis with 21.25:78.75, and MaxiGro with 15:85. Both fertilizers were maintained at the same concentration of nitrogen throughout the experiment, although the rates were changed according to developmental stage. The nutrient solutions initially contained 100 ppm N fertilizer for three weeks, followed by a water change and an increase to 150 ppm N.The pH and EC levels were closely monitored throughout the experiment. Results revealed significant differences among cultivars for all measured variables, with Salvius demonstrating superior performance in most aspects. The light source had no significant impact on aerial growth variables, while the MaxiGro fertilizer brand significantly and positively influenced plant weight, height, and root weight. Although there were significant interactions between light source, cultivar, and fertilizer for above-ground variables, these were highly dependent upon fertlizer. In conclusion, the choice of fertilizer brand is crucial for optimal lettuce growth in hydroponic systems. This study highlights the importance of selecting appropriate fertilizer characteristics to avoid detrimental effects on biomass production. Further trials are recommended to validate these findings and address concerns for home and commercial growers in hydroponic lettuce production.
Speakers
DR

Dario Rueda Kunz

Texas Tech University
Co-authors
CS

Catherine Simpson

Texas Tech University
NA
Wednesday September 25, 2024 2:00pm - 2:15pm HST
Coral 1

2:00pm HST

HEMP 2 - Variable Planting Date Influences on Growth and Development of Floral Hemp in North Dakota
Wednesday September 25, 2024 2:00pm - 2:15pm HST
Commercially produced floral hemp (Cannabis sativa L.) is high in cannabidiol (CBD) concentrations relative to tetrahydrocannabinol (THC); this is intentional due to regulatory pressures requiring low THC thresholds. Given the predominant role of genotype in plant development, it is crucial to also explore environmental factors that may allow for optimization of hemp growth, yield, and quality. The goal in this study is to evaluate the extent to which an extended vegetative growing period has on height, width, and yield in irrigated raised bed production of floral hemp in North Dakota. To survey this relationship between planting date, growth, and quality parameters, mother plants were germinated from seed at the beginning of the growing season. Each treatment group of cuttings, separated by approximately two weeks, were excised from their respective cultivar mother plant for four timing treatment groups. Cultivars evaluated were ‘ACDC’, ‘Bubbatonic’, ‘Sour Elektra’, and ‘Umpqua’. The main effect of cultivar did not significantly affect any measured parameters except for height and the top 1/3 portion of dry floral biomass. Planting date treatments significantly affected the wet weight of total above-ground biomass, total dry above-ground biomass, and total floral biomass. Average total above-ground biomass and dry floral biomass was statistically different for each planting date except for the last two planting dates (June 19 and July 3). Total above-ground dry biomass averaged over cultivars, were 4070 g, 2432 g, 1323 g, and 894 g, for dates May 19, June 5, June 19, and July 3, respectively. Mean yields for total dry floral biomass, averaged over cultivars, were 1779 g, 1279 g, 784 g, and 535 g, for dates May 19, June 5, June 19, and July 3, respectively. Earlier planting dates showed an increase in height for three of the four cultivars with the exception of ‘Umpqua’. A cultivar interaction with planting date treatment occurred for the top 1/3 portion of dry floral biomass and indicated that earlier planting date increased the biomass for ‘Bubbatonic’ and ‘Sour Elektra’ while planting date did not influence dry biomass for ‘ACDC’ and ‘Umpqua’. The insights gained from assessing the impact of variable vegetative growing periods on growth and quality parameters of photoperiod-dependent floral hemp could have broader implications for optimizing production practices. Understanding the intricate interplay between genotype, environmental factors, and cultivation practices is essential for advancing sustainable and efficient hemp cultivation strategies.
Speakers
BS

Brock Schulz

North Dakota State University
Co-authors
CA

Collin Auwarter

North Dakota State University
NA
HH

Harlene HattermanValenti

North Dakota State University
Wednesday September 25, 2024 2:00pm - 2:15pm HST
South Pacific 2

2:00pm HST

PROP - Enhancing Germination of Silver Saw Palmetto (Serenoa repens form glauca): Water Uptake, Temperature, and Pre Sowing Treatment Effects
Wednesday September 25, 2024 2:00pm - 2:15pm HST
Silver saw palmetto is a native palm of the southeast coast of the United States that is of high ornamental value for native landscapes and commercial potential production due to its fruit’s phytotherapeutic properties. The main propagation method for this palm is seed-based germination, which has very slow and low germination rates. This study assessed imbibition rates, germination in different temperatures that simulated Florida seasons, and pre-sowing treatments. Diaspores were exposed to seeds within the endocarp, intact seeds, scarified seeds, and seeds with their operculum removed. The maximum fresh weight of saw palmetto seeds after imbibition was observed at 11 days. Significant differences in imbibition rates and final weight were noted for seeds with an endocarp, with lower fresh mass, and scarified seeds, which showed the highest rates. Summer temperatures consistently yielded the highest final germination percentage and uniformity for seeds with or without an endocarp on constant summer temperatures or move-along experiments throughout Florida seasons. Pre-sowing treatments, such as the removal of the operculum, proved to be highly efficient in promoting the highest germination percentages and rates, while seeds with an endocarp demonstrated lower and uneven germination. The application of hormones, particularly GA3 at 10,000 ppm, also showed promise in improving germination rates.
Speakers
VP

Vania Pereira

University of Florida
Co-authors
HP

Hector Perez

University of Florida
NA
JO

Jena Osmani

University of Florida
NA
TS

Thiago Souza Campos

UNESP - FCAV
NA
WV

Wagner Vendrame

University of Florida
NA
Wednesday September 25, 2024 2:00pm - 2:15pm HST
Lehua Suite

2:00pm HST

VCM 3 - Optimizing Butterhead Lettuce Production in a High Tunnel Environment
Wednesday September 25, 2024 2:00pm - 2:15pm HST
The production of fast-growing vegetable crops (such as specialty type lettuces) in slightly modified environments using high tunnels are widely underutilized. These structures can be used in the Midwest for winter production of a wide variety of leafy greens, including specialty lettuce. These crops have potential to garner a market premium due to high demand when there are not many other local vegetables for sale. Therefore, a study was conducted in 2020 and 2021 to evaluate four butterhead lettuce varieties (‘Nancy’, ‘Rhapsody’, ‘Red Cross’ and ‘Skyphos’) to determine optimal density for maximum revenue generation on a 3 ft wide raised bed. Three rows per bed and at 9 in. between plants worked best for ‘Nancy’, ‘Rhapsody’ and ‘Skyphos’, while for ‘Red Cross’ (a small more erect growing variety), 4 rows per bed at 12-inch spacing between plants maximized productivity. Head diameters were also highest for ‘Nancy’, ‘Rhapsody’ and ‘Skyphos’ using the 3 row, 12 inch between plant spacings. Overall gross revenues obtained per high tunnel, depending on plant density and variety, ranged from $3,600 to $6,000 (sold per piece at $4 each) or $1,753 to $7,279 (sold by weight at $6/ lb) for a typical 26’ (wide) x 48’ (long) high tunnel. Although most revenues increased when sold by weight, some increased by 50% (e.g., Rhapsody when grown at 3 row and 12 in spacings). Additionally, ‘Nancy’ (green-leaf type) and ‘Skyphos’ (red-leaf type) tended to be of higher quality than the other varieties evaluated and would be the better varieties to grow in a lower Midwest high tunnel environment. Both ‘Nancy’ and ‘Skyphos’ had optimal gross revenues when grown at 3 rows per bed with 9 in-row spacings when sold by piece ($4,500) or weight ($6,979 and $6,426, respectively). Thus, it is important for growers to understand the importance of butterhead variety selection and plant density in a high tunnel, as well as the method sold to maximizes revenues.
Speakers
AW

Alan Walters

Southern Illinois University
Wednesday September 25, 2024 2:00pm - 2:15pm HST
Kahili

2:00pm HST

Fruit, Vegetable, and Edible Crops Collaboration Session
Wednesday September 25, 2024 2:00pm - 3:00pm HST
A forum for discussion of potential collaborations with regards to fruit, vegetable, and edible crops – i.e. citrus, breeding, production systems, postharvest, pomology, crop management, viticulture, etc.
Moderator
avatar for Jayesh Samtani

Jayesh Samtani

Associate Professor, Virginia Tech
Wednesday September 25, 2024 2:00pm - 3:00pm HST
Coral 4 - ASHS Collaboration Center

2:00pm HST

Workshop: Evaluation Methods to Assess the Drought Tolerance of Landscape Plants in the Western United States
Wednesday September 25, 2024 2:00pm - 4:00pm HST
This workshop is to introduce the coordinated network of non-biased plant trials that has been successfully established to assess plant growth and aesthetic quality under deficit irrigation in six locations with different climate and soil types. The standard evaluation method will be highlighted to allow the audience to gain a behind-the-scenes look at the success and challenges of managing a multi-year and multi-state field project titled ‘Climate Ready Landscape Plants’. The evaluation methods employed in this project could potentially be utilized for selecting climate-resilient plants in other regions of the United States and beyond.

During their presentations, participants will be invited to: 1) Learn about the process of building cooperator and stakeholder engagement for a regional multistate project. 2) Practice using an Excel-based Irrigation Log to apply deficit irrigation using reference evapotranspiration published by local weather stations. 3) Practice evaluating selected landscape plants (pictures and/or live plants purchased from Home Depot in Honolulu) using the Rubric for Plant Aesthetic Ratings developed by the UC Landscape Plant Irrigation Trials™ team. 4) Learn the standard methods for collecting plant physiology data of selected landscape plants across multiple locations and understand the project results regarding stomatal conductance, which was collected using LI-600 Porometer/Fluorometer, LI-6800 Photosynthesis System, and/or CIRAS-3/4 Portable Photosynthesis System, etc. Ventors such as LI-COR Biosciences and PP Systems will be invited to demonstrate their equipment for plant physiological measurements (e.g. stomatal conductance) during the session. 5) Learn the standard methods for collecting plant growth and visual quality data across six locations and understand the challenges and results of common taxa tested in six diverse geographic locations. 6) Learn step-by-step how to conduct open houses and invite professionals to help evaluate plants in the field trials, as well as how to collect and use the data.

Following their presentations, the six speakers along with other team members including Dr. Lloyd Nackley, Dr. Ryan Contreras, Dr. Shital Poudyal, and Dr. Youping Sun, will be invited to join a 30-min panel discussion session to further share the success and challenges of managing a multi-year and multi-state field project. They will delve into the opportunities and challenges currently facing the Green Industry. This discussion aims to foster future collaboration for expanding current research and Extension efforts. The goal is to promote the production and utilization of low-water-use plants within the green industry and among the gardening public, especially in the context of a changing climate.

Western U.S. nursery stock, bedding, annual, and perennial plant sales exceeded $2.9 billion in 2017, with nursery stock sales from this region alone accounting for 37.6% of total U.S. sales (Agricultural Statistics, 2017). However, climate change and increased urban water demand threaten the future of the Green Industry. The Western U.S. is expected to endure extreme droughts escalating in severity due to climate change, less predictable precipitation patterns, and decreased soil moisture (Cayan et al., 2010). Urban water supplies will be further stressed by population growth. The populations of Arizona, Idaho, Nevada, and Utah increased by 1.7% or more from 2017 to 2018 (United States Census Bureau, 2018). To address these challenges, growers must supply low-water-use plants and the landscape industry must utilize them to facilitate water conservation by reducing landscape irrigation requirements. In response to this need, the USDA Agricultural Marketing Service Specialty Crop Multi-State Program funded a project titled ‘Climate Ready Landscape Plants’ in 2020 to the University of California, Davis. With the funding, trial methods developed in California have been expanded to four additional western states: Arizona, Oregon, Utah, and Washington. A coordinated network of non-biased plant trials has been established to assess plant growth and aesthetic quality under three irrigation frequencies. Low-water-use plants were identified in 2022 and 2023 and will be recommended for production and utilization. The information developed will be provided to the green industry to aid in sustainable decision-making, marketing, and business support. The evaluation methods employed in this project could potentially be utilized for selecting climate-resilient plants in other regions of the United States and beyond.

Agricultural Statistics 2017. 2017. United States Department of Agriculture National Agricultural Statistics Service. https://www.nass.usda.gov/Publications/Ag_Statistics/2017/Complete%20Ag%20Stats%202017.pdf Cayan, D.R., Das, T., Pierce, D.W., Barnett, T.P., Tyree, M., and Gershunov, A. 2010. Future dryness in the southwest US and the hydrology of the early 21st century drought. Proceedings of the Natl. Acad. of Sci. 107 (50), 21271-21276. https://doi.org/10.1073/pnas.0912391107 United States Census Bureau. 2018. Nevada and Idaho are the nation’s fastest growing states. United States Department of Commerce. https://www.census.gov/newsroom/press-releases/2018/estimates-national-state.html

Coordinator(s)
  • Youping Sun, Utah State University, Department of Plants, Soils & Climate, Logan, Utah, United States
Moderator(s)
  • Lloyd Nackley, North Willamette Research and Extension Center Oregon State University, Aurora, OR, United States
Speaker/Participant(s)
  • Lorence Oki, Building Cooperator and Stakeholder Engagement for a Regional Multistate Project 
  • Jared Sisneroz, Coordinating a Standard Irrigation Protocol across Six Diverse Plant Trial Locations 
  • Karrie Reid, Introducing the Criteria for Plant Aesthetic Ratings
  • Soo-Hyung Kim, Coordinated Assessment of Physiological and Morphological Traits of Landscape Plants across Multiple Locations in the Western United States
  • Ursula Schuch, Assessing Plant Growth and Visual Quality - Challenges and Results of Common Taxa Tested in Six Diverse Geographic Locations
  • Natalie Levy, A Step-by-Step Guide for Organizing a Successful Open House Event
Moderator
avatar for Lloyd Nackley

Lloyd Nackley

oregon state university
Lloyd Nackley is a plant ecologist who applies a systems approach to improve nursery and greenhouse management. Nackley's research program at Oregon State University focuses on addressing four challenges facing nursery and greenhouse production in Oregon: irrigation application, pest... Read More →
Speakers
KR

Karrie Reid

Env. Hort Advisor, ret'd., University of California
Ms. Reid is a retired Area Environmental Horticulture Advisor. She developed the project at UC Davis in 2004 for her M.S. thesis research and served as the program lead.
SK

Soo-Hyung Kim

Dr. Kim is a Professor and Plant Ecophysiologist whose research focuses on how plants interact with their environment at the whole-plant scale. He is the principal investigator for the ‘Climate Ready Landscape Plants’ project at the University of Washington.
avatar for Natalie Levy

Natalie Levy

Soil Health and Organic Materials Management Advisor, UC Agriculture and Natural Resources
Dr. Levy was recently hired by UC Ag and Natural Resources as the Soil Health and Organic Materials Management Advisor serving Orange, Los Angeles, and San Diego counties. She was previously an Associate Specialist for Water Resources at the South Coast Research and Extension Center... Read More →
US

Ursula Schuch

University of Arizona
Dr. Schuch is a Professor and Extension Specialist in Environmental Horticulture in the School of Plant Sciences. Dr. Schuch’s research focuses on plant selection and management strategies to conserve water, maintain plant health, and aesthetic function. She is the principal investigator... Read More →
LO

Lorence Oki

University Of California, Davis
Dr. Oki is a UC Cooperative Extension Specialist Emeritus. In 2004, Dr. Oki initiated the trial research program that has developed into the current UC Landscape Plant Irrigation Trials™ (UCLPIT). He currently leads the California Department of Food Agriculture / USDA Specialty... Read More →
JS

Jared Sisneroz

UC Davis
Mr. Sisneroz is the project manager of the UC Landscape Plant Irrigation Trials (UCLPIT) and the ‘Climate Ready Landscape Plants’ programs. He specializes in landscape plant water-use efficiency research and water resource management.
Wednesday September 25, 2024 2:00pm - 4:00pm HST
Coral 2

2:05pm HST

EcoPhys/HPRB/PGR - Molecular Assessment of Heat Sensitivity in Broccoli Flowering
Wednesday September 25, 2024 2:05pm - 2:15pm HST
In the warm growing season on the East Coast, broccoli crown development is often disrupted because insufficient cold accumulation for flower bud initiation and enlargement. As part of an effort to breed for adaptation to higher growing temperatures, we investigated whether the sensitivity is due to expression of one or more of the central genes involved in flower initiation. Broccoli transitions from vegetative to reproductive phase normally, it is the transition from reproductive meristem to floral meristem and flower bud that is arrested or delayed in warm temperatures. We compared the heat response of a highly sensitive genotype, ‘Clara’ and the most resistant available genotype ‘P13xP19’ (P. Griffiths, Cornell). Plants that had just entered the reproductive phase were exposed to temperatures that were either permissive (16/12°C Day/Night) or restrictive (28/22°C Day/Night) for three days, then RNA was isolated from the meristem. The RNA was sequenced, transcripts were identified and relative abundance of each transcript was determined. Transcripts were available corresponding to the genes of interest. The model is that various developmental and environmental cues affect expression of the integrator gene SOC. The expression level of SOC then influences a gene that maintains meristem (TFL1) and one that promotes flower development (LFY). The interplay between those genes in time and space is believed to control how big the meristem will get and when the meristem will start to make flowers. When LFY expression dominates, it promotes expression of AP1 (and paralogs) inducing floral primordia. A gene responsible for the heat sensitivity would have differential expression in heat only in the sensitive genotype. That was the case for TFL1 and one copy of SOC1, but not for the other genes. Therefore, heat sensitivity is caused by genes associated with meristem transition, not with the classic flower-initiation genes.
Speakers
avatar for Thomas Bjorkman

Thomas Bjorkman

Professor Emeritus, Cornell Horticulture
How do you move from adequate cover cropping to excellent cover cropping? What fine tuning improves carbon, nitrogen, weeds, percolation, tillage goals?We all need to advocate for horticultural research and education with those who decide to spend money on our work. It is not difficult... Read More →
Co-authors
Wednesday September 25, 2024 2:05pm - 2:15pm HST
South Pacific 3

2:14pm HST

Genetics and Germplasm 2 (GG 2)
Wednesday September 25, 2024 2:14pm - 3:30pm HST
Updates on Curation and Standardization of Phenotypic and Genotypic Data for Horticultural Databases - Jill Bushakra
Identification Of Branched-Chain Amino Acid Derived Volatile Loci In Tomato (Solanum Lycopersicum) Using GWAS And WGCNA - Austin hart
From 'Agawam' to 'Zinfandel': Fruit Quality And Metabolite Diversity In The USDA Grapevine Repository - Victoria Meakem
Moving Beyond Montmorency: Exploring the Genetic Diversity of Tart Cherry - Benjamin Gutierrez
Population affects growth and plant architecture in wild-collected Hydrangea quercifolia - Lisa Alexander
Moderator
Wednesday September 25, 2024 2:14pm - 3:30pm HST
South Pacific 1

2:14pm HST

Organic Horticulture/ Bioenergy (ORGH/BioECP)
Wednesday September 25, 2024 2:14pm - 4:05pm HST
Phenotypic landscape of the photosynthetic performance and seed productivity of spinach germplasm under organic conditions - Vijay Joshi
The Southern Cover Crop Variety Trial Program - Assessing Regional Adaptation of Cover Crop Varieties Across the Southern US (Year 2) - Virginia Sykes
Production of short, medium and long season specialty crops in high-residue, no-till farming systems - Arianna Bozzolo
Comparison of Plastic Mulch and Cover Crops - Roller Crimper Systems on Weed Pressure Soil Moisture and Yield in Organic Strawberry Production - Arianna Bozzolo
Impacts of Organic Soil Amendments on Hemp Production - Arnab Bhowmik
Biomass Source of Biochar and Genetic Background of Tomato Influence Plant Growth and Development and Fruit Quality - Rishikesh Ghogare
Nitrogen Utilization from Compost in Organic Strawberries - Mark Kindred
Interactions between photoselective plastics and insect pest dynamics in an organic brassica vegetable system -Dilip Nandwani
Anaerobic Soil Disinfestation: A Non-chemical Approach to Managing Weeds and Nematodes in Sweetpotato - Simardeep Singh
Potential Establishment of Entomopathogenic Fungi as an Endophyte in Citrus Trees Over Time - Joseph Paoli
Impact of Biosolid Compost on Basil Plant Growth and Physiology - Madeleine Tenny
Moderator
avatar for Vijay Joshi

Vijay Joshi

Associate Professor, Texas A&M University System
Wednesday September 25, 2024 2:14pm - 4:05pm HST
South Pacific 4

2:15pm HST

EcoPhys/HPRB/PGR - Morpho-physiological Response of Plectranthus amboinicus under Flooding and Drought Stress
Wednesday September 25, 2024 2:15pm - 2:25pm HST
Water stress is one of the major limiting factors of crop growth and productivity worldwide. Plectranthus amboinicus (Jamaican Thyme) is an aromatic, spicy and medicinal plant which is also valued in the pharmacological industry. Although the plant is generally known to tolerate some levels of drought, its response to sequences of different water stresses is unknown. In this study, we investigated the effect of flooding and drought on the growth and development of Plectranthus amboinicus. The treatments were regular watering (RW), flooding (FL), drought (DR), and rehydration (RH) after drought. The result showed that plant height was significantly (p < 0.001) reduced in DR and FL plants while fresh aboveground weight was increased by ca. 17.4% in FL compared to RW. Stomatal conductance and transpiration rates were higher in FL plants but reduced drastically in DR. Moreover, chlorophyll fluorometric indices including maximum quantum yield efficiency of Photosystem II (Fv/Fm) and potential photosynthesis capacity (Fv/Fo) were significantly (p < 0.001) increased in the FL but decreased in DR plants. Leaf relative water content was significantly (p < 0.01) highest in the RH followed by the RW, and the least in DR and DH. Leaf cell electrolyte leakage was significantly (p < 0.05) higher in the RH followed by the RW while FL recorded the least cell electrolyte leakage. These results indicate that Jamaican thyme regulates physiological characteristics to tolerate flooding and demonstrate that DR poses a severe threat to plant development.
Speakers
SA

Samuel Asiedu

Dalhousie University
Co-authors
LA

Lord Abbey

Dalhousie University, Faculty of Agriculture
NA
RO

Rapahe Ofoe

Dalhousie University, Faculty of Agriculture
NA
SC

Sparsha Chada

Dalhousie University, Faculty of Agriculture
NA
ZW

Zijing Wang

Dalhousie University, Faculty of Agriculture
NA
Wednesday September 25, 2024 2:15pm - 2:25pm HST
South Pacific 3

2:15pm HST

ORGH/BioECP - Phenotypic landscape of the photosynthetic performance and seed productivity of spinach germplasm under organic conditio
Wednesday September 25, 2024 2:15pm - 2:25pm HST
Identifying suitable genetics for low-input organic spinach production and ensuring an adequate supply of organic seeds are critical challenges for organic spinach breeders. This study comprehensively assessed 300 spinach accessions sourced from the USDA National Germplasm Repository and commercial checks. The field trials were conducted at Uvalde, TX, during the 2022-23 and 2023-24 growing seasons, focusing on biomass and nitrogen use efficiency. During the 2023-24 field trial, natural variations in leaf photosynthetic performance, including carbon assimilation, stomatal conductance, photosynthetic electron transport rate (ETR), and PSII actual photochemical quantum yield (PhiPS2), were analyzed utilizing an LI-6800 gas analyzer. The seed productivity traits, such as yield per plant and 100-seed weight, were evaluated at the Organic Seed Alliance Washington field site. The processed data were employed for Genome-Wide Association Analysis (GWAS) to identify underlying genetics associated with organic productivity and photosynthetic traits. Over 88k Single Nucleotide Polymorphisms (SNPs) dispersed across six chromosomes were utilized in the GWAS after stringent filtering criteria. Significantly associated SNPs and candidate genes were delineated for the targeted photosynthetic traits and seed productivity, shedding light on potential genetic factors influencing these crucial traits in organic spinach breeding programs.
Speakers
avatar for Vijay Joshi

Vijay Joshi

Associate Professor, Texas A&M University System
Co-authors
avatar for Ainong Shi

Ainong Shi

Associate Professor, University of Arkasnas
Dr. Ainong Shi is a faculty member in the Department of Horticulture at the University of Arkansas. His research laboratory specializes in plant breeding and genetics, particularly focusing on vegetable crops such as arugula, cowpea, and spinach for cultivar and germplasm development... Read More →
AF

Alice Formiga

Oregon State University
NA
MC

Micaela Colley

Organic seed Alliance
NA
Wednesday September 25, 2024 2:15pm - 2:25pm HST
South Pacific 4

2:15pm HST

CE 3 - Enhancing Lettuce Yield: Strategies for Fertility Management in Soilless Growth Systems.
Wednesday September 25, 2024 2:15pm - 2:30pm HST
The focus on sustainability and effective resource management is expanding along with the upward trend in greenhouse production. Precise application of fertilizers is becoming more and more important in a variety of agricultural systems. The physical and chemical characteristics of soilless growth media differ from those of soil, which causes differences in their ability to retain nutrients. As such, accurate rates of fertilizer are crucial. This study looked at 14 different fertilizer blends with varying percentages of potassium (0-200ppm), phosphorus (0-100ppm), and nitrogen (0-200ppm). Pots were filled with Berger BM6 media and then ‘Buttercrunch’ lettuce seeds were planted. With each treatment fertilizer rate, the plants were hand-watered once a week to maintain a 10% leaching fraction. The number of leaves, dry shoot weight, fresh root weight, dried root weight, and SPAD readings were among the end measurements. The study found that a mix of high rates of nitrogen, phosphorous, potassium fertilizer treatments increased fresh shoot weight. This emphasizes the need for additional study to determine the best fertilizer rates for various specialty crops grown in soilless greenhouse environment.
Speakers
BW

Bryce Waugh

Grad Student, Oklahoma State University
Co-authors
BD

Bruce Dunn

Oklahoma State University
Wednesday September 25, 2024 2:15pm - 2:30pm HST
Coral 1

2:15pm HST

GG 2 - Updates on Curation and Standardization of Phenotypic and Genotypic Data for Horticultural Databases
Wednesday September 25, 2024 2:15pm - 2:30pm HST
The Genome Database for Rosaceae (GDR, https://www.rosaceae.org/) and the Genome Database for Vaccinium (GDV, https://www.vaccinium.org/), are databases that support genomics, genetics and breeding in under-represented crops like small fruits. These fruit crops include Fragaria (strawberry), Rubus (red raspberry, black raspberry, and blackberry) in GDR, and Vaccinium (blueberry and cranberry) in GDV. Data include curated genome sequences, genetic maps, markers, QTL, genes, transcripts, germplasm, and publications, made accessible to browse, query and download through easy-to-use web interfaces and tools. One of the objectives of a 2022-funded SCRI- project ‘Advanced National Database Resources for Specialty Crop Research and Improvement’ is to collect, curate, and integrate all types of genomics, genetics, and breeding big data in easy-to-use and robust crop-specific databases. In this presentation, we summarize our progress towards curating and making available phenotype and genotype data for strawberry. We also present a strawberry Crop Ontology we have developed with input from crop researchers and breeders from North America and Europe. Public availability of phenotypic and genotypic data in GDR, GDV, and GRIN-GLOBAL will allow easy access to this data to use in genome-wide association studies. Crop Ontology will enable digital capture and trait data integration across locations and projects.
Speakers
avatar for Jill Bushakra
Co-authors
CH

Chun Huai Cheng

Washington State University
NA
DM

Doreen Main

Washington State University
JY

Jing Yu

Washington State University
NA
JH

Jodi Humann

Washington State University
KB

Katheryn Buble

Washington State University
NA
NB

Nahla Bassil

USDA-ARS
NA
PZ

Ping Zheng

Washington State University
NA
SJ

Sook Jung

Washington State University
NA
TL

Taein Lee

Washington State University
NA
Wednesday September 25, 2024 2:15pm - 2:30pm HST
South Pacific 1

2:15pm HST

HEMP 2 - In-vitro Screening of Native Plant Crude Extracts Against Major Plant Pathogens Affecting Cannabis and Specialty Food Crops of Louisiana
Wednesday September 25, 2024 2:15pm - 2:30pm HST
Louisiana’s hot and humid climate provides the perfect environmental conditions for the growth of fungal and bacterial plant pathogens. These fungal infections are an obstacle to the success of commercial production of Cannabis sativa in the state. Two of the most recent and significant fungal diseases are southern blight caused by Sclerotium rolfsii and stem canker caused by Botrytis cinerea. However, there is a lack of formal and professional knowledge regarding fungi that infect medicinal hemp plants, and practical and effective methods for managing the casual agents of these diseases. The objective of this study was to identify natural plant products from two native plants of Louisiana, that have been reported in the Native American ethnobotanical literature to have antifungal/antibacterial properties. An in-vitro bioassay experiment was conducted using the agar plug diffusion method testing the antifungal inhibition of crude ethanol extracts from the two species against each of the two pathogens Sclerotium rolfsii and Botrytis cinerea, on four plates each of Extract 1 Diospyros virginiana L. and Extract 2 Equisetum hymale L. of 1/4PDA spiked at a dose of 250ppm, 500ppm, 750ppm, and 1000ppm against a control plate of 1/4PDA for six days. Based on this initial crude extract bioassay there is a highly significant difference in the two crude extracts (p=0.000105) when tested against Botrytis cinerea. There is also a significant difference in concentration. The test against Sclerotium rolfsii did not find any significant inhibition from either of the plant extracts tested. From our findings we will continue the research study to test the antifungal potential of crude ethanol extract as well as Hexane, chloroform, ethyl acetate soluble fractions of Extract 1 Diospyros virginiana. The goal of the study is to integrate the antifungal compounds and their application for the development of best practices in Cannabis production.
Speakers
avatar for Jennifer Blanchard

Jennifer Blanchard

Instructor Horticulture - Medicinal Plants (PhD Student), Louisiana State University
Jennifer Blanchard is a botanist and Instructor of Horticulture in the School of Plant Environmental and Soil Sciences (SPESS) at LSU. She has created a new course at the university in Louisiana Medicinal Plants that focuses on the native indigenous plants of the state. She is also... Read More →
Wednesday September 25, 2024 2:15pm - 2:30pm HST
South Pacific 2

2:15pm HST

PROP - Propagation Strategies and Epigenetic Variations in Vaccinium Berry Crops
Wednesday September 25, 2024 2:15pm - 2:30pm HST
Blueberry (Vaccinium sect. Cyanococcus Rydb.), lingonberry (V. vitis-idaea L.) and huckleberry (V. membranaceum; also called black huckleberry or black blueberry) are three important Vaccinium small fruit crops native to the North. They are valuable resources for bioactive components that play important roles in anti-oxidant, anti-tumor, anti-ulcer, and anti-inflammatory activities. They can be propagated to produce true-to-type plants either conventionally or by micropropagation. Propagation in vitro is now well accepted worldwide for commercial production of Vaccinium crops. Proficient plant propagation technique using shoot organogenesis and somatic embryogenesis was successfully established in blueberry and lingonberry. Shoot regeneration and proliferation in in-vitro conditions using various liquid bioreactors have successfully been achieved in various Vaccinium species. One of the major concerns in commercial production is the occurrence of variation in micropropagated plants. This presentation describes the in-depth progress of genetic and epigenetic variations in different Vaccinium species during micropropagation. It addresses different molecular techniques used to monitor true-to-type and epigenetics along with application of epigenetic variation in micropropagated Vaccinium berry crops. Keywords: blueberry, bioreactor micropropagation, DNA-methylation, huckleberry, molecular markers, lingonberry, somaclonal variation
Speakers
avatar for Samir Debnath

Samir Debnath

St. John’s Research and Development Centre
Samir Debnath, Ph.D., P.Ag. Dr. Samir C. Debnath, a Research Scientist of Agriculture and Agri-Food Canada and an Adjunct Professor of Biology, Memorial University of Newfoundland, has authored and co-authored around 140 publications in peer-reviewed journals including review papers... Read More →
Co-authors
MS

Mehdi Sharifi

Summerland Research and Development Centre, AAFC, 4200 Highway 97, Summerland, British Columbia, Canada
Dr. Mehdi Sharifi is a “soil nutrient management” research scientist at Summerland Research and Development Centre in British Columbia, Canada. Before his current position, he was a professors at Trent and Dalhousie Universities. He completed two postdoctoral fellow positions... Read More →
RB

Rajesh Barua

Department of Biology, Memorial University of Newfoundland, St. John's, Newfoundland and Labrador, Canada
NA
SK

Sayani Kundu

Department of Biology, Memorial University of Newfoundland, St. John's, Newfoundland and Labrador, Canada
NA
SM

Sweety Majumder

Department of Biology, Memorial University of Newfoundland, St. John's, Newfoundland and Labrador, Canada
NA
US

Umanath Sharma

Department of Biology, Memorial University of Newfoundland, St. John's, Newfoundland and Labrador, Canada
NA
Wednesday September 25, 2024 2:15pm - 2:30pm HST
Lehua Suite

2:15pm HST

VCM 3 - Resource Use Efficiency, Yield, and Quality of Amaranth Grown as a Summer Leafy Green in Alternative Soilless Systems
Wednesday September 25, 2024 2:15pm - 2:30pm HST
Soilless growing systems (SGS) integrated into controlled environment agriculture (CEA) offer a viable solution for increasing food production in densely populated areas with limited land, water, and nutritional security. The design, components, inputs, and nutrient management of alternative SGS along with the plant genotype, directly impact crop yield, nutritional value, and resource use efficiency. As the adoption of CEA and SGS continues to expand, there is a growing need to compare and evaluate the performance of alternative SGS in terms of resource use efficiency, crop yield, and impact on crop nutritional quality. To this purpose, a study was undertaken to assess the performance of five alternative SGSs including Deep Water Culture (DWC), Nutrient Film Techniques (NFT), Ebb and Flow, Drip irrigation, and the Kratky system concerning yield, nutritional quality, and resource use efficiency, using green and red stripe amaranth cultivar as test crops. As expected, the Kratky system had the lowest dissolved oxygen levels in the nutrient solution compared to other SGSs. Among the growing systems, DWC and NFT exhibited higher fresh and dry biomass production compared to drip irrigation, ebb and flow, and the Kratky system. There was no distinct difference in leaf fresh and dry weight between cultivars, but green amaranth displayed higher stem fresh and dry weight than red stripe amaranth. Dry matter content remained unaffected by the SGS, while red stripe amaranth exhibited higher dry matter content compared to green amaranth. The Kratky and DWC systems utilized 34.2% and 25.6% less nutrient solution, respectively, compared to the ebb and flow system, which had the highest nutrient solution consumption compared to other methods. DWC had the highest water use efficiency (WUE, dry biomass produced per liter of water used). Nitrate content in amaranth leaves was higher when grown in NFT compared to drip irrigation, DWC, and ebb and flow, but was similar to Kratky. The red stripe cultivar displayed higher nitrate content than the green one. Regarding bioactive compounds, green amaranth exhibited higher total antioxidants and flavonoids, whereas DWC and NFT led to lower total phenols and flavonoids compared to the Kratky, ebb and flow, and drip irrigation systems. In conclusion, the DWC and NFT systems maximized yield, with DWC having higher water use efficiency, while Kratky, ebb and flow, and drip irrigation system resulted in higher nutritional quality of the amaranth grown as a summer leafy vegetable.
Speakers
PP

Pradip Poudel

The Penn State University
Pradip
Co-authors
FD

Francesco Di Gioia

The Pennsylvania State University
NA
Wednesday September 25, 2024 2:15pm - 2:30pm HST
Kahili

2:25pm HST

EcoPhys/HPRB/PGR - Effects of Paclobutrazol, Progressive-raising Temperature and Spike-truncated Treatments on Phalaenopsis Join Grace ‘TH288-4’
Wednesday September 25, 2024 2:25pm - 2:35pm HST
Phalaenopsis is the most popular potted plant worldwide. However, its long spikes often lead to increased shipping costs and risks. This study investigates the effectiveness of varying the concentration, timing, and frequency of paclobutrazol (PP333) applications on shortening the spike of Phalaenopsis Join Grace ‘TH288-4’. Also we examine on different progressive-raising temperature promoting inflorescence development for prior anthesis, and on spike-truncated treatment optimizing flowering performance. Three experimental groups were established based on different PP333 application schedules: T2, T2T3 and T7T8 group. The PP333 concentrations used were 0, 250, 500, 750, and 1000 mg·L-1, applied as foliar sprays. The shortest spikes, measured from base to first flower, were observed in the T2 group with 750 and 1000 mg·L-1; the T2T3 group treated with 500, 750, and 1000 mg·L-1 PP333; and the T7T8 group treated with 1000 mg·L-1. These treatments resulted in spike lengths of 16.7-22.2 cm, which are 54-69% shorter than the control ones. PP333 application had minimal effects on other traits except root diameter. Subsequently, four experimental groups: G20, G25, G30, and G35 group, were set up based on different final flower induction temperature. Mature plants were applied with 750 mg·L-1 PP333 by foliar spray, after four weeks moved to a cool room as 20/18℃. Until the flower stalk bore 0-3 nodes, the G25, G30 and G35 groups were transferred to 25/20℃. While the flower stalk bore 4-5 nodes, the G30 and G35 group were moved to 30/25℃. When the flower stalk bore 6-7 nodes, the G35 group was finally cultured under 35/30℃. Half of plants were randomly selected and truncated the spikes while the first flower bud was 1-1.5cm long. The spike of the G35 and G30 were truncated on the 21st week, which took only 113 and 117 days from the day started placing at a cool room. The G25 and G20 group took 124 and 138 days respectively. The days to flowering of G35 and G30 group were less than G20 at least by 45 days. However, the flower of G35 group performed unusual and were the smallest. All flowers of spike-truncated plants were bigger than the controls under different temperatures. This study establishes a PP333 treatment and a flower induction temperature protocol, offering a strategy to effectively produce single-flowered phalaenopsis.
Speakers
avatar for Yi Chien Rivana Lu

Yi Chien Rivana Lu

National Chaiyi University
Co-authors
ML

MingHua Lee

National Chiayi University
NA
RS

RongShow Shen

National Chiayi University
NA
Wednesday September 25, 2024 2:25pm - 2:35pm HST
South Pacific 3

2:25pm HST

ORGH/BioECP - The Southern Cover Crop Variety Trial Program - Assessing Regional Adaptation of Cover Crop Varieties Across the Souther
Wednesday September 25, 2024 2:25pm - 2:35pm HST
Cover crops provide numerous ecological benefits to production systems. Maximizing these benefits requires identifying species and varieties that are the best fit both geographically and for the planting/termination time frame dictated by the accompanying cash crop system. In 2019, the University of Tennessee began a cover crop variety trial to help growers make better informed decisions on which of a broad range of cover crop species and varieties worked best in their specific region and production system. In fall 2022, in collaboration with the Southern Cover Crops Council, this trial was expanded to cover a 10-state region of the Southern US (TN, FL, NC, KY, VA, SC, TX, GA, AR, and AL) and became known as the Southern Cover Crop Variety Trial. This trial program is currently in its second year, with each trial year running from fall through the following spring. Trial sites were planted in mid-October each year. Cover crop cover, weed suppression, height, biomass, and estimated nitrogen release were measured in early and late spring. Twenty entries each were evaluated in the 22-23 and 23-24 trial years. However, as a fee-based program, entries were submitted by public and private institutions and varied from year to year. Eleven of the twenty entries were evaluated in both trial years. In year one, top-performers by rank were largely uniform across sites, consisting primarily of cereal rye and hairy vetch varieties. Many clover species, particularly crimson clover, exhibited greater variation in performance ranking among locations. Performance values varied significantly among and within locations, species, and evaluation period. Data collection for year two will be completed in Spring 2023 and will be presented.
Speakers
VS

Virginia Sykes

University of Tennessee
Co-authors
AT

Alyssa Thelin

University of Tennessee
NA
AW

Alyssa Woodard

North Carolina State University
NA
AM

Amanda Mcwhirt

University of Arkansas
AG

Audrey Gamble

Auburn University
NA
CC

Carlene Chase

University of Florida
NA
CR

Chris Reberg Horton

North Carolina State University
NA
DB

Daniel Boakye

University of Florida
NA
DT

Danielle Treadwell

University of Florida
NA
DM

David McIntosh

University of Tennessee
NA
EH

Erin Haramoto

University of Kentucky
NA
EH

Esleyther Henriquez Inoa

North Carolina State University
FP

Francisco Palacios

University of Tennessee
NA
GM

Gabriel Maltais Landry

University of Florida
NA
LS

Lakesh Sharma

University of Florida
Dr. Lakesh Sharma is an assistant professor of soil fertility and sustainable agriculture at the University of Florida in Gainesville, FL. Lakesh has been farming since he was a child on his own farm. His academic school journey started in 2000. He is currently working on nutrient... Read More →
LW

Lexie Worley

University of Tennessee
NA
MR

Mark Reiter

Virginia Tech
NA
NB

Nick Basinger

University of Georgia
NA
NR

Nithya Rajan

Texas A
NA
RS

Ricardo St Aime

Clemson University
NA
RH

Rick Hitchcock

University of Georgia
NA
SB

S Brooke Keadle

University of Tennessee
NA
SN

Sruthi Narayanan

Clemson University
NA
Wednesday September 25, 2024 2:25pm - 2:35pm HST
South Pacific 4

2:25pm HST

Cultivate your career with a graduate degree at the University of Arkansas
Wednesday September 25, 2024 2:25pm - 2:55pm HST
The Department of Horticulture has 16 research, teaching and extension faculty members and about 40 full-time staff plus additional hourly workers located primarily in Fayetteville, Little Rock, and at research stations in Clarksville and Kibler, AR. The department is home to approximately 15 graduate students and 75 undergraduate students and focuses on sustainable horticulture, landscape and ornamentals, fruit, vegetable, and ornamental/landscape field and controlled environment production, and turfgrass science.  The faculty members work throughout the state, have strong relationships at different Division of Agriculture research stations and work directly with producers and other relevant stakeholders. There are several exciting collaborations and novel research and extension opportunities immediately available. Among the six faculty who are actively seeking graduate students, there are projects in ornamentals, public horticulture, physiology, fruit breeding and production, integrated pest management in entomology and weed science, and controlled environment horticulture.  
Speakers
Wednesday September 25, 2024 2:25pm - 2:55pm HST
Coral 5 - ASHS Hort Theater

2:29pm HST

Pomology 2/Horticultural Plant Reproductive Biology/Intellectual Property Rights (POM 2/HPRB/IPR)
Wednesday September 25, 2024 2:29pm - 4:00pm HST
Do Plant “Growth Regulators” Really Regulate Growth? Plant Development And Plant Growth Are Not Synonymous. - Ted DeJong
Using GDR to Enable Rosaceae Research - New Data, Functionality and Future Direction - Dorrie Main
Raspberry Cultivar Evaluation Trial in Mississippi - Apphia Santy
Evaluating Sufficiency Levels and Peach Leaf Analysis for Fertilizer Decision-Making - Juan Carlos Melgar
Common Mechanisms Controlling Fruit Shapes may be Mediated by Changes in Cell Wall Properties - Easther van der Knaap
Pomological Nomenclature: Recent Developments and Problems - David Karp
Moderator
DK

David Karp

University of California, Riverside
Wednesday September 25, 2024 2:29pm - 4:00pm HST
Nautilus

2:30pm HST

CE 3 - Effect of ZnO and SiO2 Nanoparticles on Growth and Physiology of Hydroponic Lettuce Under Salinity Stress
Wednesday September 25, 2024 2:30pm - 2:45pm HST
Nanoparticles have unique physical and chemical properties, which can positively and negatively impact crop growth and tolerance to abiotic stresses. This study evaluated the potential of ZnO and SiO2 nanoparticles in alleviating salinity stress in hydroponically cultivated lettuce. Two-week-old lettuce seedlings (Lactuca sativa cv. Green Forest) were transplanted into a 5-L deep water culture system and grown for four weeks in a customized growth chamber set at 25°C with 230 µmol/m2/s PPFD. The nutrient solution was maintained at an electrical conductivity (EC) of 1.5 dS/m and pH 5.8, and replenished weekly. A factorial design was employed with four salinity stresses (non-saline, 50 mM NaCl, 33.3 mM CaCl2, 25 mM NaCl 16.6 mM CaCl2) and three nanoparticle treatments (no nanoparticle, 100 ppm ZnO, 100 ppm SiO2). Under non-saline conditions, both ZnO and SiO2 treatments showed no significant differences in shoot growth compared to the control plants. However, ZnO application reduced shoot biomass, leaf area, SPAD, chlorophyll fluorescence and net photosynthetic rate under CaCl2 and NaCl CaCl2 stress. SiO2-treated plants had higher SPAD than the control plants under CaCl2 stress but presented lower values under NaCl CaCl2 stress. Root growth also showed contrasting results based on the stress conditions. SiO2 application resulted in increased root dry weight, total root length and surface area under non-saline and CaCl2 stress, while they decreased under NaCl stress. Similarly, ZnO application enhanced root growth under non-saline conditions, but demonstrated negative effects under all salinity stress conditions. In conclusion, SiO2 nanoparticle application did not improve salinity tolerance in lettuce, except for root growth under CaCl2 stress, and ZnO nanoparticle treatments showed phytotoxicity in both shoots and roots under all salinity stress conditions.
Speakers
CL

Chungkeun Lee

Texas A&M University
Co-authors
Wednesday September 25, 2024 2:30pm - 2:45pm HST
Coral 1

2:30pm HST

GG 2 - Identification Of Branched-Chain Amino Acid Derived Volatile Loci In Tomato (Solanum Lycopersicum) Using GWAS And WGCNA
Wednesday September 25, 2024 2:30pm - 2:45pm HST
A major focus in plant breeding has been the improvement of crops through various traits that affect disease resistance and yield. However, the focus on productivity has led to an inattentiveness to other traits that specifically affect produce quality. An example of a critical fruit quality trait is its flavor, contributing to our perception of aromatic volatiles. Even at nanomolar concentrations, aromatic volatiles can be perceived by the olfactory system and influence the liking of the fruit. The focus of this study was to investigate the genetic aspect of the branched-chain amino acid (BCAA) volatiles, derived from L-valine, L-isoleucine and L-leucine, in tomato fruits. It is generally considered that these BCAA-derived volatiles contribute positively to overall liking, because these are essential amino acids required by the human diet. To identify quantitative trait loci (QTLs) affecting the biosynthetic pathway for 11 BCAA-derived volatiles, a Genome-Wide Association Study (GWAS) was conducted using a diverse and unique panel of 167 tomato accessions. The GWAS was run using the FarmCPU model in GAPIT-R, with a total of 21,893,681 SNPs, 2,735,297 INDELs, and 154 structural variants across the genome. Furthermore, a weighted gene co-expression network analysis (WGCNA) was conducted in parallel to identify modules of co-expressed genes that cluster with known genes that affect the BCAA-derived volatile pathway. A total of 113 QTLs were identified from GWAS and 3024 co-expressed genes were identified from the WGCNA. Candidate genes were screened based on annotated biochemical function, overlap within the GWAS QTLs, and gene expression in the red-ripe fruits. This led to the identification of two candidate genes, one on the long arm of chromosome 1 and another on the long arm of chromosome 11. These genes are being knocked-out using CRISPR-Cas9, and current progress is aimed to confirm the validity and function of these genes in the near-future.
Speakers
AH

Austin Hart

University of Georgia
Co-authors
DT

Denise Tieman

University of Florida
EV

Esther van der Knaap

Institute of Plant Breeding, Genetics, and Genomics, University of Georgia
NA
MS

Manoj Sapkota

University of Georgia
NA
Wednesday September 25, 2024 2:30pm - 2:45pm HST
South Pacific 1

2:30pm HST

HEMP 2 - Investigating Fiber Hemp Seed Size Impact on Germination, Emergence, and Early Growth Rate
Wednesday September 25, 2024 2:30pm - 2:45pm HST
Fiber hemp (Cannabis sativa L. <0.30 % total THC) seed represents the highest input cost for growers, as it is often imported and seeding rates are high to create an ideal stem diameter for quality purposes. Seed size in other agronomic crops can impact germination rate and early season growth. Hemp seed size varies tremendously among hemp genotypes. We evaluated the impact of seed size on germination rate, emergence, and seedling growth and physiology in two Chinese industrial hemp varieties Yuma 1 and Zhongma. We found that seed size impacted germination and radicle length in both varieties. However, these results did not translate into significant differences on emergence rate, photosynthetic parameters, or harvested plant biomass in the greenhouse. We did observe differences in net photosynthetic rate, transpiration rate, and stomatal conductance between varieties. Further research should evaluate season-long plant performance and economics based on seed size. In doing so we can optimize production standards and consequently farmer profitability.
Speakers
SC

Samantha Carroll

Graduate Research Assistant, North Carolina State University
Co-authors
AC

Amanda Cardoso

North Carolina State University
NA
DS

David Suchoff

North Carolina State University
NA
RY

Rong Yin

North Carolina State University
NA
Wednesday September 25, 2024 2:30pm - 2:45pm HST
South Pacific 2

2:30pm HST

POM 2/HPRB/IPR - Do Plant “Growth Regulators” Really Regulate Growth? Plant Development And Plant Growth Are Not Synonymous.
Wednesday September 25, 2024 2:30pm - 2:45pm HST
Much scientific literature refers to plant development and growth as though they are synonymous. While plant physiology texts (E.g., Taiz et al. 2015) and horticulture texts (E.g., Sansavini et al. 2019) emphasize the roles of various plant hormones in coordinating plant development, they simultaneously refer to them as plant growth regulators. On the other hand, the same texts emphasize assimilation processes and the important role of carbohydrate and nutrient availability as well as water relations in enabling growth to occur. The terms growth and development are often used interchangeably and the literature rarely emphasizes the difference between plant development and plant growth. This causes confusion and a lack of clear thinking when attempting to develop explanations for plant growth responses in specific circumstances. Hormone physiologists often try to explain particular growth responses in terms of hormonal theory whereas environmental physiologists will likely explain the same responses in terms of environmental conditions and availability for the resources required for achieving growth. In this paper I will argue for a clearer differentiation between plant development and plant growth and suggest that plant hormones should not be thought of as plant growth regulators, but rather as plant development coordinators. Plant development coordinators (plant hormones) set up the conditions necessary for plant growth but availability of plant growth substances; carbohydrate and nutrient availability along with temperature and water relations, are often what actually regulate plant growth rates. Treating development and growth as separate but interdependent processes could clarify much understanding of the underlying processes involved in the regulation of plant growth. These concepts will be discussed in the context of understanding the mechanisms involved in several physiological phenomenon of fruit trees.
Speakers
TD

Ted DeJong

UC Davis
Wednesday September 25, 2024 2:30pm - 2:45pm HST
Nautilus

2:30pm HST

PROP - The Application Timing of a Cytokinin B-Mo-based Product Affects the Growth and Non-Structural Carbohydrates Contents of Cuttings During Adventitious Root Development
Wednesday September 25, 2024 2:30pm - 2:45pm HST
A large group of horticulture species are propagated vegetatively through shoot-tip cuttings harvested from stock plants and planted to form adventitious roots. Adventitious root development (ARD) leads to establishing a carbohydrate sink in the region of root regeneration, which is highly dependent on energy and carbon skeletons. We hypothesized that enhancing the source-to-sink relationship during ARD can lead to an efficient carbon allocation to growth, resulting in a well-rooted cutting. Thus, the research aimed to determine if the application time of a Cytokinin B-Mo-based product during the ARD of unrooted cuttings would impact the source-to-sink relationship and, hence, affect plug growth and quality. The product was applied at four application times plus a negative control as follows: T1: plants without product (control), T2: sticking stage (starting 24 h after the sticking); T3: callus formation stage; T4: root development stage; and T5: toning stage. The experiment was a complete randomized design with five treatments and 60 repetitions for treatment (n=60) in two experimental runs (n=120). The root and shoot length, the number of leaves, the leaf chlorophyll content, the root and shoot dry matter, and the root-to-shoot ratio were measured. Results indicate that applying the product at T2 in ARD's dedifferentiation and induction phase leads to the shortest root and shoot length, the lowest dry matter accumulation, and some phytotoxicity effects. For T3 in ARD's initiation phase, the application results in greater root, total dry matter, and total soluble sugar contents than the control. For T4 in ARD's expression phase, the application results in greater growth, total dry matter, and sucrose and starch contents than control and other treatments. Applying the product in T5 when the roots had grown and reached all the edges of the growing media did not have any benefit compared to the control. Our results suggest that the exogenous application of a product to strengthen the source-to-sink relationship during adventitious root development can potentially enhance the non-structural carbohydrate contents when applied at the root formation phase (T4), which correlates positively with the increase in the length and dry matter of the roots, generating a well-toned rooted cutting.
Speakers
avatar for Mayra Toro Herrera

Mayra Toro Herrera

Postdoctoral Research Associate, University of Connecticut
Co-authors
RR

Rosa Raudales

University of Connecticut
Wednesday September 25, 2024 2:30pm - 2:45pm HST
Lehua Suite

2:30pm HST

VCM 3 - Differential performance of Radicchio cultivars in New Hampshire
Wednesday September 25, 2024 2:30pm - 2:45pm HST
Radicchio (Cichorium intybus var. latifolium) is grown widely throughout Europe, especially northern Italy. This crop features several groups that differ in color, shape, and culinary use, and there is considerable phenotypic variation among and within groups and cultivars. The New England growing climate is very different from that of major production regions in Europe and the pacific northwestern U.S. The availability of varieties to commercial growers in the U.S. has expanded greatly, but there remains a need for regionally relevant information about performance of these varieties. In Durham, NH, USA we grew 30 (2022) and 34 (2023) cultivars of radicchio belonging to seven main groups (Chioggia, Castelfranco, Treviso, Sugarloaf, Verona, Rosa del Veneto, and Lusia). In both years, they were seeded on 20-21 June, and transplanted on 14-15 July. Plants were harvested and weighed as they reached market maturity, or considered unmarketable due to bolting, rot, tipburn, or failure to head. We used standard cultural practices for the region: plants were grown in double rows on 30-inch-wide raised beds covered with white-on-black plastic mulch with drip irrigation. The weather in 2022 and 2023 differed greatly, with 2022 experiencing warm and dry conditions, and 2023 much wetter and cooler. The percentage of heads that became marketable ranged from 0-94% in 2022, and from 10-95% in 2023. Several cultivars had very high percentages of unmarketable heads, due to tipburn, rot, bolting, or failure to head. We also observed cultivar differences in susceptibility to powdery mildew. In both years, the cv. Rubro developed powdery mildew early and developed severe symptoms, several others eventually developed symptoms, and some never did. For traditional round red Chioggia types, several cultivars had very high percentages of marketable heads, and appear to be well adapted to production in the northeast. We identified cultivars within both sugarloaf and Treviso types that were well adapted, with high percentages of marketable heads. The cultivars we evaluated within the Lusia and Rosa del Veneto types were especially interesting from a culinary and attractiveness point of view, but had high percentages of rot (Lusia types) and very long days to maturity required (Rosa del Veneto types).
Speakers
avatar for Becky Sideman

Becky Sideman

Member-At-Large
Co-authors
LH

Lilly Hartman

University of New Hampshire
MH

Mark Hutton

University of Maine
PG

Peyton Ginakes

University of Maine
Wednesday September 25, 2024 2:30pm - 2:45pm HST
Kahili

2:30pm HST

Endowment Fund Committee Meeting
Wednesday September 25, 2024 2:30pm - 3:30pm HST
Moderator
GN

Genhua Niu

Texas A
Speakers
avatar for Esmaeil Fallahi

Esmaeil Fallahi

ASHS Board of Directors Chairman; ASHS President 2022-2023, University of Idaho
ASHS Board of Directors Chairman; ASHS President 2022-2023;Professor of Pomology and Viticulture
avatar for Curt Rom

Curt Rom

University Professor. And Associate Director, University of Arkansas and ASHS
avatar for Sandra Wilson

Sandra Wilson

Professor, University of Florida
Dr. Sandra Wilson is a Professor of Environmental Horticulture at the University of Florida’s main campus in Gainesville. She received B.S. and M.S. degrees from the University of Delaware and a Ph.D. in Plant Physiology from Clemson University. Dr. Wilson completed postdoctoral... Read More →
Co-authors
Wednesday September 25, 2024 2:30pm - 3:30pm HST
Sea Pearl 3

2:35pm HST

ORGH/BioECP - Production of short, medium and long season specialty crops in high-residue, no-till farming systems
Wednesday September 25, 2024 2:35pm - 2:45pm HST
When speaking to diversified vegetable crop growers, one of the most often cited challenges is the management of weeds. This issue is even more prevalent for organic growers who lack any effective chemical weed control method and rely on crews to mechanically remove weeds from the fields. One possible alternative is the use of terminated cover crops as mulch on the soil surface to act as a physical barrier to prevent weed emergence. This cover crop mulch can be advantageous for a grower in addition to cover crops improving soil health and providing a habitat for beneficial insects. This experiment compared vegetable beds with cover crops terminated with a roller crimper to bare soil beds on short (squash), intermediate (pepper), and long (eggplant) growing vegetable crops and assessed crop performance. In both 2023 and 2024 a cover crop mix of rye and field peas was successfully terminated and provided ample biomass on the soil surface at the time of vegetable transplanting and throughout their grower and harvest. We found that cover cropped treatments led to improvements in soil fertility, higher numbers of soil-dwelling beneficial insects, and lower weed pressure that led to significantly higher yields. These results demonstrate a potential system for organic growers to integrate into their operation as a practice that can reduce time and labor costs for weed management along with improving the soil fertility and creating a system requiring fewer inputs.
Speakers
avatar for Arianna Bozzolo

Arianna Bozzolo

Rodale Institute
Co-authors
AS

Andrew Smith

Rodale Institute
NA
JP

Jacob Pecenka

Rodale Institute
NA
Wednesday September 25, 2024 2:35pm - 2:45pm HST
South Pacific 4

2:45pm HST

ORGH/BioECP - Comparison of Plastic Mulch and Cover Crops - Roller Crimper Systems on Soil, Weeds and Yield in Strawberry Production
Wednesday September 25, 2024 2:45pm - 2:55pm HST
While organic production of specialty crops has been highly successful in southern California, it relies heavily on the use of plastic mulch and intensive tillage for weed management. A two-year study conducted from 2022 to 2023 aimed to evaluate the impact of plastic mulch and a cover crop based reduced tillage system using a -roller crimper to terminate cover crops on weed pressure and yield in organic strawberry production. Bare root strawberry crowns of the Albion variety were transplanted into four treatments: 1) Sorghum-sudangrass (S. bicolor x S. sudanense) seeded at 100 kg/ha (Sorghum 1), 2) Sorghum-sudangrass seeded at 200 kg/ha (Sorghum 2), 3) buckwheat seeded at 67 kg/ha, or 4) plastic mulch as standard practice. Cover crops were broadcasted on 1.5 m wide raised beds in May and terminated with a roller crimper in August. Both seeding rates of Sorghum-sudangrass effectively suppressed weed growth before and after termination compared to buckwheat. The significantly lower biomass of buckwheat after termination, relative to the Sorghum-sudangrass treatments, indicates variations in weed suppression efficacy among cover crops. Total differences in soil moisture, calculated based on data collected weekly from after cover crop termination to end of harvest, indicate that all treatments resulted in lower soil moisture levels compared to the plastic control, with the buckwheat treatment showing the most significant decrease. At harvest there were no significant differences between plastic mulch and Sorghum-sudangrass 1 and 2 treatments, indicating comparable yields. Buckwheat resulted in the lowest yield, experiencing a 50% reduction compared to plastic mulch. Based on the findings, plastic mulch and Sorghum-sudangrass planted at different seeding rates can be considered effective alternatives for weed suppression and maintaining yields comparable to conventional plastic mulching. The choice between Sorghum-sudangrass seeding rates may depend on factors such as cost, availability, and specific field conditions. The reduced biomass production of buckwheat compared to Sorghum-sudangrass, coupled with its rapid decomposition rate, resulted in diminished efficacy of buckwheat in weed suppression. Consequently, reduced weed control was observed, leading to significantly lower yields in buckwheat-treated plots compared to Sorghum-sudangrass and control treatments. These findings suggest that buckwheat may not be a viable option for organic strawberry production under comparable conditions, emphasizing the importance of selecting cover crops with longer-lasting weed-suppressive qualities.
Speakers
avatar for Arianna Bozzolo

Arianna Bozzolo

Rodale Institute
Co-authors
AS

Andrew Smith

Rodale Insitute
NA
JP

Jacob Pecenka

Rodale Institute
NA
Wednesday September 25, 2024 2:45pm - 2:55pm HST
South Pacific 4

2:45pm HST

CE 3 - An Intermediate Calcium-mobilizing Biostimulant Concentration Controls Tipburn of Two Greenhouse Hydroponic Lettuce Cultivars Without Affecting Growth
Wednesday September 25, 2024 2:45pm - 3:00pm HST
Lettuce (Lactuca sativa) tipburn is a physiological disorder that leads to unappealing browning or necrosis of young leaf tips and stems, caused by localized calcium deficiency. It negatively impacts crop quality and yield, making proactive management essential for achieving optimal production. The objective was to evaluate the efficacy of a calcium-mobilizing chemical biostimulant, applied in the nutrient solution, on lettuce growth and tipburn. We conducted a greenhouse experiment on two lettuce cultivars (‘Dragoon’ and ‘Rex’) using a randomized complete block design. The seedlings were grown indoors under continuous white light from light-emitting diodes (LEDs) with a mean daily light integral (DLI) of 26 mol⋅m−2⋅d−1. We transferred 11-day-old seedlings to deep-water-culture hydroponic trays in a greenhouse. The two cultivars were subjected to three replications and five biostimulant concentrations (BC) of 0 (control), 0.125, 0.25, 0.5, and 1 mL⋅L−1 of the nutrient solution. Plants were grown under an 18-h photoperiod with a mean DLI of 16.6 ± 2.0 mol⋅m−2⋅d−1 from both sunlight and supplemental white LEDs, an air temperature of 24.6 ± 3.1 °C, and relative humidity of 33.2% ± 9.5%. Plant data were collected 14, 21, 28, and 35 days after transplant (DAT). There was no visible tipburn 14 DAT; however, plant diameter and shoot mass (fresh and dry) decreased with increasing BCs. We observed tipburn 21 DAT in both cultivars. The control had the highest severity on a 0–5 scale (0 = no tipburn; 5 = severe tipburn) for ‘Dragoon’ (0.6) and ‘Rex’ (1.3), whereas no tipburn occurred under higher BCs (i.e., 0.5 and 1 mL⋅L−1). Tipburn progressed 28 DAT, when increasing the BC from 0 to 1 mL⋅L−1 decreased the tipburn rating from 3.3 to 0 for ‘Dragoon’ and from 4.1 to 0 for ‘Rex’. Plant growth was stunted under the highest BC (i.e., 1 mL⋅L−1). At 35 DAT, both cultivars had severe tipburn under the control but had decreasing tipburn severity as the BC increased. Plant growth was unaffected under the control and low BCs (i.e., 0, 0.125, 0.25 mL⋅L−1). Under the highest BC, ‘Dragoon’ had the longest roots, but ‘Rex’ had the shortest. In contrast, plants experienced phytotoxicity (reduced biomass and chlorophyll concentration) under the highest BC, i.e., (1 mL⋅L−1) though no tipburn was recorded. In conclusion, the optimal calcium-mobilizing BC was 0.5 mL⋅L−1, which minimized tipburn of greenhouse hydroponic lettuce without affecting biomass accumulation or causing phytotoxicity during later development stages.
Speakers
QM

Qingwu Meng

University of Delaware
Co-authors
SM

Shem Msabila

University of Delaware
Wednesday September 25, 2024 2:45pm - 3:00pm HST
Coral 1

2:45pm HST

GG 2 - From 'Agawam' to 'Zinfandel': Fruit Quality And Metabolite Diversity In The USDA Grapevine Repository
Wednesday September 25, 2024 2:45pm - 3:00pm HST
The USDA National Plant Germplasm System is a network of germplasm repositories dedicated to conserving genetic diversity of crops and their wild relatives. The USDA grapevine (Vitis) repository contains 5000 unique accessions representing 36 species, and is divided between two locations: Davis, CA and Geneva, NY. While this material is available for distribution to researchers and breeders, there is currently limited characterization data to help requestors identify accessions with unique and valuable traits, particularly for fruit quality. Thus, we began a germplasm screening project to measure fruit quality traits of 481 unique accessions from both locations spanning three years (2022-2024). Fruit samples were juiced, filtered through cheesecloth, and analyzed for Brix, titratable acidity, and available nitrogen. Additionally, composition of phenolic compounds was assessed using liquid chromatography-mass spectrometry (LC-MS), and aromas were detected using solid phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (GC-MS). Overall, there was a wide range of diversity in fruit quality traits across the collection. Brix values ranged from 8.5 to 30.1 (average=17.8), and titratable acidity ranged from 2.7 to 25.6 g mol-1 Tartaric acid equivalents (average=8.2). The phenolic compounds commonly detected in grape juice included the anthocyanins malvidin 3-O-glucoside and peonidin 3-O-glucoside, the hydroxycinnamic acid caftaric acid, and the flavonols quercetin 3-O-glucuronide and isoquercetin. Analysis of aromatic compounds revealed accessions that contained higher amounts of linalool, β-myrcene, and geraniol, which are associated with a “Muscat” flavor profile, while others contained methyl anthranilate, which is associated with a “Foxy” flavor profile. We hope this fruit quality dataset not only proves to be a valuable asset to researchers utilizing the USDA Vitis repository, but may also open new directions of exploration into improved grape flavor, nutrition, and quality.
Speakers Co-authors
Wednesday September 25, 2024 2:45pm - 3:00pm HST
South Pacific 1

2:45pm HST

HEMP 2 - Modified Media and Lighting for Repeated In Vitro Cutting Cycles of Cannabis Sativa
Wednesday September 25, 2024 2:45pm - 3:00pm HST
Micropropagation usually involves cytokinin in single-harvest batches. We report two in vitro studies with multiple harvest: (1) fed batch process with modified physical states and (2) LED light treatments. In (1), genotypes of Cannabis sativa were observed in stationary agar (A), stationary Oasis® infused with liquid (OIL) and agitated Oasis® infused with liquid (AOIL).Fifteen explants were planted in vessels with 120 mL DKW medium harvested on 3-week cycles, with 0 or 15 mL additional media. Harvested shoots, length, and dry mass from repeated cycles were recorded. Genotypes T1 and Peach failed on multiple harvest cycles and were eliminated, although single cycle had higher quality in OIL. BaOx and Cherry1 on OIL/AOIL with additions were better quality than A in five cycles. Shoots harvested increased from 15 to 30 in cycles 1-3 in OIL/AOIL, but in A were approximately 20, while length was longest in OIL/AOIL. By cycle 3, all measured responses were decreasing until cycle 5 where a minimum of 7 shoots per vessel or more were only in OIL, but shoots were too short to plant in greenhouse. In (2), blue and supplemental far-red were observed with in vitro shoots of BaoX and Cherry1. OIL treatments were placed in LED polychromatic and dichromatic light (white, high red:blue, medium red:blue, white w/5% far-red, high red:blue 5% far-red, medium red:blue w/5% far-red, low red:blue w/ 5% far-red) at similar intensities (190-240 µmols·m-2·s-116 h-photoperiod). Media additions were made with responses recorded bi-weekly. Five randomly selected microcuttings per vessel rooted ex vitro on mist bench for 16 days. Over multi-cycles, plants treated with 5% far-red increased number and length, while plants under higher blue light increased dry mass. Shoot number increased to 28 in cycles 1-3 with far-red, and 18 without before decreasing to initial 15 during cycle 5. The accumulated shoots per vessel over 5-cycles (10-weeks) was 108 with far-red, and 84 without. Shoot length in far-red-treated plants increased from 19 - 25 mm in cycle 3 before decreasing to 10 mm in cycle 5. Plants without far-red had 10 – 15 mm length the entire experiment. Dry mass was highest during cycle 1 with blue light before decreasing 50% in cycle 3, where it remained until cycle 5. Sixty-eight percent of shoots rooted regardless of prior in vitro treatment. OIL with media addition allowed shoots to be harvested five cycles, while signaling response of far-red light allowed increased productivity and length.
Speakers
MM

Molly McKay

Clemson University
Co-authors
EL

Elizabeth Luscher

Curio Wellness
NA
JF

James Faust

Clemson University
NA
JA

Jeffrey Adelberg

Clemson University
NA
MT

Matt Taylor

Curio Wellness
RK

Raghupathy Karthikeyan

Clemson University
NA
Wednesday September 25, 2024 2:45pm - 3:00pm HST
South Pacific 2

2:45pm HST

POM 2/HPRB/IPR - Using GDR to Enable Rosaceae Research - New Data, Functionality and Future Direction
Wednesday September 25, 2024 2:45pm - 3:00pm HST
Initiated in 2003, the Genome Database for Rosaceae (GDR, www.rosaceae.org) is a comprehensive community database that provides access to curated and integrated genomics, genetics, and breeding data for the biologically and economically important Rosaceae family. It serves as steward of critical research and breeding data, and provides access to online query and analysis tools that enable researchers to readily interrogate this wealth of data, facilitating basic and applied research across Rosaceae. This presentation will highlight the impact of GDR on Rosaceae research, demonstrate new data and tools, and share plans for future development and sustainability options.
Speakers
avatar for Dorrie Main

Dorrie Main

Professor, Washington State University
I am a member of the Pullman Charter School Initiative team and am particularly interested in how to set up and maintain a thriving Washington State Charter School to serve the needs of Eastern Washington students.
Wednesday September 25, 2024 2:45pm - 3:00pm HST
Nautilus

2:45pm HST

PROP - Characterizing the effects of PPFD and VPD during indoor acclimation of Chrysanthemum cuttings
Wednesday September 25, 2024 2:45pm - 3:00pm HST
During acclimation of unrooted cuttings (URC) under mist irrigation, the moisture status of URC is greatly affected by environmental conditions such as high photosynthetic photon flux density (PPFD) and vapor pressure deficit (VPD), both of which can cause rapid dehydration. The objective of this study was to characterize the effects of PPFD and VPD on indoor acclimation of Chrysanthemum ‘Olympia White’ URC. Cuttings were stuck in a peat-perlite substrate in propagation trays, placed under sole-source lighting in chambers where ultrasonic fog was used to automatically control relative humidity (RH). Mist irrigation was applied every 20 to 30 min for 3 h. Over 12 h, URC were exposed to 0, 35, 70, 80, 105, 140, or 210 µmol·m–2·s–1 PPFD and 70, 80, 90, or 100% RH setpoints, resulting in air VPD levels ranging from 0.02 to 0.59 kPa. In the chambers, there was a high level of environmental control, and PPFD did not affect air VPD (P = 0.712), which were therefore considered as independent climatic factors. Leaf temperature minus air temperature was considered an indicator of plant energy balance, where a warmer leaf than air temperature is a driver of evapotranspiration. Plant leaf temperature became cooler than the air temperature as air VPD increased (P = 0.024), likely caused by evaporative cooling of leaves. In contrast, increasing PPFD increased leaf temperature relative to air temperature, likely through radiant heating. Leaf VPD was positively correlated with air VPD (adjusted-R2 = 0.88) but was also affected by the interaction of PPFD and air VPD (P < 0.05). Two hours after misting was terminated (5 h total), some plants showed clear wilting especially under low RH. At 5 h, stomatal conductance and evapotranspiration of URC was increased by PPFD and decreased by air VPD (P < 0.01), although there was an interaction between PPFD and air VPD for stomatal conductance (P < 0.005). Further results highlighting responses to RWC, water uptake, and water loss will be presented.
Speakers
AS

Ana Sofia Gomez

Purdue University
Co-authors
CG

Celina Gomez

Purdue University
PF

Paul Fisher

University of Florida
NA
Wednesday September 25, 2024 2:45pm - 3:00pm HST
Lehua Suite

2:45pm HST

VCM 3 - Comparative Analysis of Biodegradable Mulch Use in Hill-side Strawberry Production
Wednesday September 25, 2024 2:45pm - 3:00pm HST
Comparative analysis of biodegradable mulch use in hill-side strawberry production Plastic film (PF) is widely used in crop production, yet global environmental concerns urge for reduced plastic waste. Biodegradable and bio-based mulches emerge as promising alternatives, potentially crucial for sustainable agriculture. This significance is emphasized by the hypothesis that small farms prioritize sustainability and seek to reduce plastic waste in soil and food systems, especially if these alternatives match or exceed the benefits of plastic mulch. However, there is limited data available on the effectiveness of biodegradable paper (BP) and bio-based film (BB) in small-scale crop production, an area frequently affected by heat and drought stress. A field experiment was conducted on a strawberry field in Redlands, San Bernardino County, California during the seasons of 2022 and 2023, respectively. This study was conducted to identify the most appropriate alternative to plastic mulch in hill-side production systems under the Mediterranean climate as it relates to yield and fruit quality at harvest day. We used four common mulches in small farms in California, polyethylene mulch (PE), landscape paper mulch (PA), Coconut liner mulch (CL), Biodegradable plastic mulch (MB), and bare soil (control) with the most popular ever-bearing variety, ‘Albion’. To investigate the suitability and efficacy of his mulches, we recorded the soil properties (temperature, moisture, and pH) daily, fruit yield (fruit weight and fruits/plant) biweekly, and fruit quality (Soluble solid and color) at harvest considering farm stand or U-pick sale service. Our results indicate that the highest total yield in terms of total fruit weight and number of fruits per plant grows under MB, CL, and PF mulch respectively. No notable differences were observed among treatments in fruit quality parameters such as soluble solids and color. The soil under the coconut coir liner had the highest moisture content, compared to PE, BDM, and bare ground treatments. No significant difference was found among the treatments in weed control. By the end of the cropping period, all treatments adequately covered the soil surface. However, in the buried part of the mulches, the PA mulch exhibited considerable degradation before the end of the season, while the MB showed faster signs of biodegradation. Based on crop productivity and the different mulch efficacy observed in our study, this production system with CL and MB is a good alternative to compare to PE and PA mulches for ever-bearing hillside strawberry production in the Mediterranean climate.
Speakers
avatar for Amrita Mukherjee

Amrita Mukherjee

University of California Agriculture and Natural Resources (UCANR)
Co-authors
NP

Nolton Pattio

Jehovah Jireh Farm
NA
Wednesday September 25, 2024 2:45pm - 3:00pm HST
Kahili

2:55pm HST

ORGH/BioECP - Impacts of Organic Soil Amendments on Hemp Production
Wednesday September 25, 2024 2:55pm - 3:05pm HST
Hemp (Cannabis sativa L.) is a highly versatile crop that has attracted considerable attention among farmers due to its diverse applications. Recent studies have sought to establish a fundamental understanding and baseline of the nutritional requirements of hemp, opening up possibilities for organic hemp production. Our research focuses specifically on evaluating the productivity of a floral hemp variety using different regenerative practices, with a particular emphasis on organic soil amendments that promote soil health in Piedmont area of North Carolina. The field experiment was conducted at North Carolina A
Speakers
AB

Arnab Bhowmik

Associate Professor, North Carolina A&T State University
Co-authors
DR

Dipti Rai

North Carolina A
NA
KW

Kennedy Wimbish

North Carolina A
NA
Wednesday September 25, 2024 2:55pm - 3:05pm HST
South Pacific 4

3:00pm HST

CE 3 - Optimizing Sampling Methods for Sap Extraction to Enhance Plant Nutrient Analysis in CEA
Wednesday September 25, 2024 3:00pm - 3:15pm HST
Plant sap analysis is a technique for monitoring plant nutrient status in real-time, enabling precise nutrient management to enhance growth and yield in controlled environment agriculture (CEA). Comprehensive sampling techniques are vital for accurate determination of nutrient concentrations, considering the variability of nutrients across different developmental phases of plants. However, questions remain regarding the selection of the appropriate plant tissues, including the number of leaves collected, sampling time, type and age of plant tissue, and frequency. Different crops need specific sampling procedures due to their unique leaf morphology, growth habits, and physiology. Many commercial laboratories only distinguish between new and old leaves. In this series of studies, we determined the most effective sampling method including the number of leaves, the type and age of tissue, as well as the timing and frequency of the collection. Optimal sampling techniques were identified for lettuce and tomato by conducting five different experiments across three cultivars. These experiments varied the number of leaves sampled (10, 20, 30 per sample with three replicates), types of tissue (leaves for lettuce with three replicates, and petioles and leaves for tomatoes with 20 each per sample), age of tissue (new vs. old with 20 leaves per sample and three replicates), time of collection (6, 8, 10 am with three replicates). For lettuce, two developmental stages (half and final harvest maturity), while for tomatoes, sampling frequency at four different growth stages was investigated (first fully expanded leaves, 1/3 and 2/3 of crop development, and final harvest). The results indicate that collecting 20 fully expanded leaves at 8 am, particularly at the final harvest, was considered the best sampling technique for nutrient analysis for both lettuce and tomatoes, providing the most effective sampling technique for optimizing nutrient management.
Speakers
HR

Husnain Rauf

University of Georgia
Co-authors
DJ

Daniel Jackson

University of Georgia
NA
JL

Jason Lessl

University of Georgia
NA
JS

Jessica Staha

Local bounti
NA
MP

Miguel Puebla

Pure flavor
NA
RS

Rhuanito S. Ferrarezi

University of Georgia
NA
TC

Timothy Coolong

University of Georgia
ZR

Zilfina Rubio

University of Georgia
Wednesday September 25, 2024 3:00pm - 3:15pm HST
Coral 1

3:00pm HST

GG 2 - Moving Beyond Montmorency: Exploring the Genetic Diversity of Tart Cherry
Wednesday September 25, 2024 3:00pm - 3:15pm HST
Genetic diversity is invaluable to the sustainability of American horticulture. In the case of tart cherry, production in the United States is precariously reliant on a single cultivar, ‘Montmorency.’ Our research explores diverse genetic resources in tart cherry to promote utilization of high quality and locally adapted cultivars for plant breeding and improved production. Tart cherry nutritional quality is of particular interest to consumers. The United States Department of Agriculture Tart Cherry collection in Geneva, New York maintains 100 cultivars of tart cherry, including their wild relatives. Over a five-year period, we assayed fruit quality traits, including Brix, titratable acidity, and phenolic content. Total soluble solids (TSS) ranged from 10.9 to 20.7% (average=14.8%) and acidity (TA) ranged from 5.3 to 32.1 g/L (average=16.3%). The sugar/acid ratio ranged from 3.7 to 27.6 (average=10.2). Individual fruit weight ranged from 0.2 to 8.6 g (average of 5.0 g) and pit weight percentage ranged from 6-32% (average=11%). Total anthocyanin content varied from 75.2 to 3760.0 μg/g, with an average of 771.4 μg. We also evaluated bloom phenology over a three-year period. The distribution of bloom ranged from 56.7 to 134.4 GDD, with an average value of 86.0 Growing Degree Days (GDD ). ‘Montmorency’ bloom was above average with values around 95.8 GDD. Sweet cherries (63.5 – 90.6 GDD) tended to bloom much earlier than tart cherries (64.9 –118.0 GDD) and P. fruticosa, the wild progenitor of the tart cherry, bloomed the latest with a range of 85.3 to 134.4 GDD. For Brix, acidity, and phenolic content, ‘Montmorency’ falls significantly below average, though it has a balanced sugar/acid ratio. ‘Montmorency’ is lacking in anthocyanin content which is increasingly relevant for the juice industry. It tends to bloom later than other tart cherries evaluated, though there are some more extreme late bloomers. This data will be available through GRIN-Global, the USDA germplasm database to facilitate future research and breeding.
Speakers Co-authors
Wednesday September 25, 2024 3:00pm - 3:15pm HST
South Pacific 1

3:00pm HST

HEMP 2 - QTL mapping and gene discovery for seed traits in hemp (Cannabis sativa L.) F2 mapping populations
Wednesday September 25, 2024 3:00pm - 3:15pm HST
The emergence of a thriving hemp industry in the U.S. will depend on the breeding of high-yielding regionally adapted cultivars. Despite the latest research efforts, little is known regarding the genetic basis of important agronomic traits in hemp. The objective of this research was to identify and characterize genomic regions associated with seed morphology and quality traits. F 2 mapping populations were developed by crossing hemp germplasm bred or cultivated for cannabinoids (‘FL 58’ × ‘TJ’s CBD’), grain (GVA- H-20-1179 × ‘Picolo’), or fiber (‘Si-1’ × GVA-H-21-1003) market classes. These populations were investigated due to their variation in seed size and seed crude protein. The cannabinoid, grain, and fiber populations were grown and seed was harvested in 2021, 2022, and 2023, respectively. Harvested seeds were phenotyped for thousand seed weight (TSW) and crude protein content predicted by near-infrared (NIR) spectroscopy. The high-cannabinoid population was genotyped using an Illumina array, while the fiber and grain populations were genotyped using an Agilent SureSelect Custom Target Enrichment Probe Set. Marker-associated sequences were aligned to the CBDRx v.2.0 reference genome to align the physical and genetic maps. The TSW and protein content in the cannabinoid population ranged from 9.62 to 23.93 g and 19.25 to 31.89 %, respectively. In contrast, the TSW of the fiber and grain populations ranged from 7.34 to 45.17 g and 8.73 to 31.42 g, respectively. Numerous quantitative trait loci (QTL) of varying effect sizes were identified genome-wide. Notably, in the high- cannabinoid population, major and minor effect QTL for TSW were detected on Chr01 corresponding to 642 kb and 5.56 Mb genetic regions, respectively. Our results in the cannabinoid population highlight the importance of developing more than one F 2 mapping population in a given cross to capture the effect of more alleles due to high heterozygosity in hemp and evaluating distinct pedigrees to sample additional alleles in diverse genetic backgrounds. Narrowing the region around or identifying candidate genes will allow the development of high-throughput molecular markers for beneficial alleles across mapping pedigrees. These findings will accelerate hemp breeding programs through the implementation of marker-assisted selection for high-yielding and high-quality hemp cultivars for grain production.
Speakers
LM

Luis Monserrate

Cornell University
Co-authors
AW

Alexander Wares

Cornell University
NA
DW

Dustin Wilkerson

Cornell University
NA
GS

George Stack

Cornell University
NA
JT

Jacob Toth

Cornell University
NA
LS

Larry Smart

Cornell University
NA
LV

Lucia Vignale

Cornell University
NA
MQ

Michael Quade

Cornell University
NA
Wednesday September 25, 2024 3:00pm - 3:15pm HST
South Pacific 2

3:00pm HST

POM 2/HPRB/IPR - Raspberry Cultivar Evaluation Trial in Mississippi
Wednesday September 25, 2024 3:00pm - 3:15pm HST
Many states in the US produce raspberries, however, most of the production is concentrated in three states: California, Oregon and Washington as most raspberry cultivars grow best in regions with cool summers and mild winters. However, newer raspberry cultivars have been developed exhibiting heat tolerance. Cultivars with heat tolerance provide an opportunity for the growers in the Southern states to include raspberries in their crop production. Local Mississippi growers are interested in incorporating raspberries into their productions. However, there lacks research-based recommendations on raspberry cultivars suitable for Mississippi's climate. The objective of this study was to evaluate raspberry cultivars in terms of plant growth, heat and cold tolerance, pest and disease resistance, berry yield, quality, and fruiting season to identify the best-suited cultivars for Mississippi. This experiment was conducted in a randomized complete block design with two types of fertilizer: conventional and organic. Data collection included measurements of plant growth and performance, berry yield and quality and fruiting season. The results showed that raspberry yield, single berry weight and fruit size were influenced by fertilizer treatment. The soluble solid contents, acidity, and fruit color were not influenced by fertilizer treatment. Raspberry yield was higher for “Polka”, “Encore”, “Heritage”, and “Latham” under conventional fertilizer. Cultivars “Himbo”, “Prelude”, ‘Bp1”, and “Encore” treated with conventional fertilizer had higher single berry weight. The average fruit size of cultivars “Prelude”, “Himbo”, “Encore”, “Bp1” treated with conventional fertilizer produced larger fruits in comparison to the other cultivars. The fruit's soluble solid content was highest in “Heritage”, indicating a sweeter taste. Cultivars “Polana” and “Anne” produced fruits with the highest acidity, indicating a tarter taste compared to other cultivars. Fruit color varied between cultivars, with differences in lightness, redness, and yellow coloration.
Speakers
avatar for Apphia Santy

Apphia Santy

Graduate Student, Mississippi State University
Hello! I'm passionate about the world of horticulture and am eager to explore and discuss various opportunities within the industry. I am particularly interested in pursuing a PhD in horticulture, with a specialization in either specialty crops or ornamentals. Additionally, I have... Read More →
Co-authors
GB

Guihong Bi

Mississippi State University
NA
TL

Tongyin Li

Mississippi State University
NA
Wednesday September 25, 2024 3:00pm - 3:15pm HST
Nautilus

3:00pm HST

PROP - Asexual Propagation of Salix humilis Using Dormant Hardwood Cuttings After Stock Plant Coppicing and Fungicide Application
Wednesday September 25, 2024 3:00pm - 3:15pm HST
Prairie willow (Salix humilis) is a naturally compact and adaptable shrub willow native to the eastern United States. This taxon has potential for use in managed landscapes because of its desirable habit, attractive flowers and foliage, and its purported tolerance to fluctuations in soil moisture. This adaptability could be advantageous for urban green infrastructure applications. However, unlike most willows, S. humilis is thought to exhibit recalcitrance to standard asexual propagation techniques. This recalcitrance is exacerbated by observed susceptibility to fungal infections that cause cutting mortality during propagation. Because juvenility can improve the rooting of propagules, we questioned whether stock plant rejuvenation (coppicing) and propagule treatment with fungicide would influence rooting and survival of S. humilis dormant hardwood cuttings. In February 2024, 300 full-length dormant stems were harvested at the USDA Plant Introduction Station in Ames, Iowa from stock plants that were either coppiced or not coppiced the previous year. Uniformly-sized basal cuttings were acquired by removing the proximal 23 cm of each stem. Experimental trials (N=300; n=75) evaluating the full factorial of stock plant status [non-coppiced (-coppice) or coppiced ( coppice)] and fungicide application [non-treated (-fungicide) or treated ( fungicide) by submerging propagules in T-Bird with 46.2% Thiophanate-methyl for 15 minutes] were conducted in a cool greenhouse at the University of Minnesota Horticultural Research Center in Chaska, MN. All cuttings were treated with 0.8% indole-3-butyric acid via Hormodin-3 Talc powder and stuck in 50 cell trays (5 × 5 × 12.5 cm cells) with a 1:1 perlite:bark-based media and placed on heat mats (22ºC) using a completely randomized design. Total number of propagules rooted, length of the longest three roots, and number of roots were recorded. Propagules were ranked on their overall rooting performance using a Likert Scale. Rooting percentage was 77%, 56%, 87%, and 69% for coppice/ fungicide, coppice/-fungicide, -coppice/ fungicide, and -coppice/-fungicide, respectively. Compared to the non-coppiced propagules, mean count of roots decreased by 41% for coppiced propagules, across fungicide applications. Compared to propagules not treated with fungicide, mean count of roots increased by 19% for propagules treated with fungicide, across coppice treatments. Future research will evaluate different types of cuttings to generate a complete vegetative propagation protocol for Salix humilis. This work supports the introduction of this versatile plant into commercial production, thereby augmenting the ornamental plant palette for challenging urban landscapes.
Speakers
HS

Hazel Schrader

Graduate Research Assistant, University of Minnesota Twin Cities
Co-authors
BM

Brandon Miller

University of Minnesota Twin Cities
Wednesday September 25, 2024 3:00pm - 3:15pm HST
Lehua Suite

3:00pm HST

Exploring Global Plant Science Opportunities: Fulbright Program with Dr. Tanisha M. Williams
Wednesday September 25, 2024 3:00pm - 5:00pm HST
Ready to dig deeper into your passion of the science of cultivated plants and take your career to new heights? Join us for an engaging session where Dr. Tanisha M. Williams, a prominent plant biologist and Fulbright U.S. Student alum, will share how the Fulbright Program can be your gateway to global opportunities. Imagine traveling the world, researching climate impacts on plant species, or exploring conservation efforts in diverse ecosystems. This is your chance to learn how Fulbright can help you cultivate essential career competencies, build lasting international connections, and prepare for a dynamic career in a multicultural world. Don’t miss this opportunity to enrich your academic journey and grow your professional network.

Dr. Tanisha M. Williams is an Assistant Professor in Plant Biology and Director of the UGA Herbarium. Her research uses an integrative approach to explore the impacts climate change has on plants found throughout South Africa, Australia, and the United States. Fulbright alum, she also founded Black Botanists Week to amplify diverse voices in science. Dr. Williams earned her Ph.D. in Ecology and Evolutionary Biology from the University of Connecticut in 2019.
Speakers
avatar for Tanisha Williams

Tanisha Williams

Assistant Professor in Plant Biology and Director of the UGA Herbarium, University of Georgia
Wednesday September 25, 2024 3:00pm - 5:00pm HST
Coral 5 - ASHS Hort Theater

3:05pm HST

ORGH/BioECP - Biomass Source of Biochar and Genetic Background of Tomato Influence Plant Growth and Development and Fruit Quality
Wednesday September 25, 2024 3:05pm - 3:15pm HST
The evolving effects of climate change and intensive agricultural practices have adversely affected soil health and crop yields. This necessitates the need to maintain and improve soil health for sustainable food production. Biochar, a carbon-rich material produced from pyrolysis of biomass is being considered as a novel soil amendment that can potentially restore soil health and improve crop yields. Several studies have reported both positive and negative effects of biochar on crop yields while focusing mainly on one type of biochar and a single plant cultivar. However, the effects of different types of biochar and its interaction with plants with different genetic backgrounds has remained unexplored. In this study, we evaluated the impact of six different biochars on the growth performance and fruit quality of three tomato (Solanum lycopersicum) cultivars. The hypotheses that (1) biochars derived from different feedstock sources would produce unique phenotypes in a single cultivar of tomato, and (2) single feedstock-derived biochar would produce different phenotypes in each of the three tomato cultivars were supported by obtained results. This study demonstrated that plant genetic background and biomass source are important variables to consider for using biochar as a soil amendment.
Speakers Co-authors
AD

Amit Dhingra

Texas A
NA
DI

Daylen Isaac

Washington State University
NA
ET

Elvir Tenic

Washington State University
NA
JL

June Labbancz

Texas A
NA
NR

Norman Richard Knowles

Washington State University
NA
Wednesday September 25, 2024 3:05pm - 3:15pm HST
South Pacific 4

3:15pm HST

ORGH/BioECP - Nitrogen Utilization from Compost in Organic Strawberries
Wednesday September 25, 2024 3:15pm - 3:25pm HST
Strawberries are typically grown in high tunnels in the Intermountain West region to protect the flowers and buds from early and late-season frosts and extend the growing season. Organic farmers rely on N mineralization from organic sources to supply plant-available N which can be inadequate for plant needs, especially when temperatures are low. To support cultivar selection for organic strawberry production in high tunnels, greenhouse cultivar trials were conducted to determine if some strawberries grow better on compost than others. Plants were grown for six weeks in a mixture of turface and peat moss with and without compost. A complete liquid fertilizer with and without added N was supplied daily. Leaf, crown, and root dry weights, tissue, and compost N at the beginning and end of each trial were measured and leachate was collected daily and analyzed for nitrate and ammonium. Plant chlorophyll was determined at the end of the experiment. Plants that received liquid N fertilizer grew more, absorbed more N, and had a higher chlorophyll reading compared to plants that received the compost and N-free fertilizer treatment. Ammonium was leached out of the compost-treated pots in the first two weeks while little nitrate was lost. Some cultivars performed better than others on the compost treatment. This suggests that some strawberry cultivars are better able to grow on organic nitrogen sources than others but, additional N fertilization may be needed.
Speakers
MK

Mark Kindred

Grad Student, Utah State University
I am a graduate student at Utah State University working on my master's degree in Dr Jennifer Reeve's lab. I got my bachelor's in plant science and Asian studies from Utah State University. While I was an undergrad, I worked in Jennifer Reeve's lab. When I graduated, I was offered... Read More →
Co-authors
BB

Brent Black

Utah State University
NA
JR

Jennifer Reeve

Utah State University
NA
RS

Robert Schaeffer

Utah State University
NA
Wednesday September 25, 2024 3:15pm - 3:25pm HST
South Pacific 4

3:15pm HST

CE 3 - Effects of Potassium and Iron Supplements and Late Nitrogen Restriction on Aquaponic Taro (Colocasia esculenta) Corm Production
Wednesday September 25, 2024 3:15pm - 3:30pm HST
The growing of taro in aquaponic systems has yielded corms significantly smaller than those grown terrestrially. Previous trials only partially supported the hypothesis that these low yields were due to excessive water and nitrogen levels late in vegetative development. A 2×2 (nitrogen restricted × supplemental fertilizer) factorial designed experiment was replicated 4 times in dual-tub systems. The 4 treatments tested were: 1) Fish effluent supplied throughout 10 months of plant development (T1); 2) Fish effluent restricted from the system at 6 months and fresh water supplied for the remaining 4 months of development (T2); 3) T1 plus supplemental potassium and iron fertilizer (T3); 4) Treatment 2 plus supplemental potassium and iron fertilizer (T4). The results indicate that the supplemental fertilizer was more important than effluent restriction late in development in enhancing corm growth, although effluent restriction did result in a higher maturity index of corms under supplemental fertilizer treatment. The corm yields were 140% higher in T3 (1.5 kg plant-1) than in T2 (0.63 kg plant-1). T4 had significantly more biomass partitioned into the corm (56% of total biomass) compared to T3 (44% of total biomass). The ratio of corm: total biomass is a key indicator of plant maturity and suggests restriction of high nitrogen effluent enhanced photosynthate translocation to the corm under supplemental fertilizer. Corm density was highest in T3 and lowest in T4, perhaps due to starch conversion to sugar in over-mature corms in T4. These results demonstrate the importance of supplementing potassium and iron fertilization, as well as restricting high nitrogen fish effluent late in taro corm development, to optimize taro yields and quality in aquaponic production systems.
Speakers
avatar for Andrew Bohringer

Andrew Bohringer

University of Hawai'i at Manoa
Co-authors
BK

Bradley Kai Fox

University of Hawai'i at Manoa
NA
EC

Eric Collier

University of Hawai'i at Manoa
NA
KW

Koon-Hui Wang

University of Hawai'i at Manoa
NA
RP

Robert Paull

University of Hawai'i at Mānoa
NA
TR

Theodore Radovich

University of Hawai’i at Mānoa
Wednesday September 25, 2024 3:15pm - 3:30pm HST
Coral 1

3:15pm HST

GG 2 - Population affects growth and plant architecture in wild-collected Hydrangea quercifolia
Wednesday September 25, 2024 3:15pm - 3:30pm HST
Hydrangea quercifolia, oakleaf hydrangea, a flowering shrub native to woodlands of the southeastern United States. Oakleaf hydrangea has immense ornamental potential with four-season interest, including traits like showy panicles, striking foliage textures, red fall color, and exfoliating bark. Cultivars are often derived from wild selections either directly or only a few generations removed. Full genetic and phenotypic variation has not been evaluated for the species, and little is known about the diversity in horticulturally important traits for oakleaf hydrangea. For this study, growth and plant architecture of wild-collected oakleaf hydrangea seedlings were observed at the Otis L. Floyd Nursery Research Center in McMinnville Tennessee over a 3-year period. Seedlings from 14 populations of oakleaf hydrangea spanning the species’ native range were planted in a randomized complete block design containing six blocks and nine replications per block. Two-way ANOVA was used to partition variation in height, width, growth rate, and number of stems into sources attributable to block, population, and block × population. There were significant differences among populations for growth rate, size, and number of stems in all years. Southern populations were smaller than northern populations and showed a slower growth rate. Genetic and phenotypic variation shown among populations will guide conservation efforts and supplement breeding efforts for oakleaf hydrangea.
Speakers
avatar for Lisa Alexander

Lisa Alexander

Research Geneticist, USDA-ARS U.S. National Arboretum
Co-authors
AS

A. Sherwood

USDA-ARS, Agricultural Research Service, North Central Regional Plant Introduction Station
CJ

C. Jennings

Tennessee State University
SH

S.C. Hokanson

University of Minnesota
Wednesday September 25, 2024 3:15pm - 3:30pm HST
South Pacific 1

3:15pm HST

HEMP 2 - Dynamics of Cannabinoid Accumulation and Morphological Changes in Cannabis Inflorescences
Wednesday September 25, 2024 3:15pm - 3:30pm HST
Cannabis (Cannabis sativa L.) is cultivated for its cannabinoids, which have applications for therapeutic and recreational use. This phenomic evaluation explores accumulation of 16 cannabinoids of interest and associated morphological changes in Cannabis flowers. Eight cultivars of interest were grown in containers within an environmentally controlled greenhouse for 150 days (72 days reproductive). Light intensity, light duration, temperature, and relative humidity were regulated. Monitoring floral development, we observed a consistent increase in cannabinoid concentration as flowers matured, peaking in advanced stages of development. This accumulation pattern was consistent across diverse cultivars, which indicates this accumulation pattern to be the result of a fundamental biological mechanism. Concurrent with cannabinoid accumulation, we noted morphological changes in trichomes, which are widely utilized as markers of maturation within industry. Trichomes transitioned from sparse and translucent to abundant, enlarged, and displaying orange/amber hues as flowers matured, signifying floral maturation and trichome senescence. Importantly, a significant linear correlation emerged between cannabinoid accumulation and trichome morphological changes across all cultivars. This underscores a tight relationship between cannabinoid biosynthesis and trichome development, shaped by genetic factors. In summary, our findings demonstrate the intricate relationship between cannabinoid accumulation and floral morphology in Cannabis. Insights gained are invaluable for cultivar selection, breeding, and cultivation practices aimed at optimizing cannabinoid quantity and time to harvest. Understanding the underlying molecular mechanisms of cannabinoids promises tailored approaches for the optimization of cannabinoid production and the fostering of therapeutic and industrial advancements in Cannabis.
Speakers
SD

Samuel des Bordes

Ph.D. Candidate, Louisiana State University
Co-authors
BJ

Babitha Jampala

Louisiana State University
NA
HK

Heather Kirk Ballard

SPESS
Heather Kirk-Ballard is an Assistant Professor and state extension specialist of Consumer Horticulture for the LSU AgCenter. She is the host of the Get It Growing ™ extension program focusing on home gardening that is disseminated weekly through video to news stations and in print... Read More →
Wednesday September 25, 2024 3:15pm - 3:30pm HST
South Pacific 2

3:15pm HST

POM 2/HPRB/IPR - Evaluating Sufficiency Levels and Peach Leaf Analysis for Fertilizer Decision-Making
Wednesday September 25, 2024 3:15pm - 3:30pm HST
The increasing demand for tree fruit production necessitates optimizing nutrient balance in intensified orchard systems to maximize profits efficiently. While peach growers are advised to follow Extension and recommended guidelines for fertilization, such recommendations may not align with orchard-specific variables and environmental conditions. As a consequence, crop sufficiency ranges may require updating to reflect modern growing practices and environmental factors. Although leaf nutrient analysis is the most reliable method for diagnosing tree nutritional status, the prevalence of annual fertilizer application, driven by the low cost of fertilizers relative to crop value, often leads to excessive fertilization in peach orchards. Consequently, our objective was to evaluate established sufficiency levels and leaf analysis as tools for determining the need for annual fertilizer applications. To achieve this, we implemented a two-year study involving two fertilization programs in an orchard with three rows of 17 peach trees: two rows adhered to grower standard, annual fertilization, while the remaining row followed a rational fertilization program. The latter implied applying fertilizer only when leaf analyses indicated nutrient concentrations below established sufficiency thresholds for peaches. Leaf analyses were conducted annually in July, and if nutrient concentrations were within or exceeded sufficiency thresholds, no fertilizer was applied postharvest or the following spring. If nutrient concentrations fell below sufficiency thresholds associated with a significant difference in yield and fruit quality between the two programs, fertilization occurred in late summer and during bloom time the following spring. We assessed tree quality and productivity by measuring yield (total weight of all the fruit per tree) and fruit quality (size and brix) annually. The results of the first year showed that despite deficient leaf nitrogen and phosphorus concentrations and other nutrients such as potassium, calcium, and magnesium remaining within or above their sufficiency ranges, we observed no significant differences in yield or fruit quality between trees subjected to rational and standard fertilization practices. Consequently, fertilization for the upcoming year was deemed unnecessary in trees following the rational program. The outcomes of this study are expected to guide peach growers in making informed decisions based on updated data, reducing the environmental impact of overfertilization, which is inefficient for fruit production and uneconomical, and enhancing farm profitability.
Speakers
avatar for Richardson Bien Aime

Richardson Bien Aime

Graduate student, Clemson University
- Plant and environmental sciences- Horticulture- Optimization of Peach Fertilization- Peach rootstocks- Agricultural economics- Sports- Music
Co-authors
JC

Juan Carlos Melgar

Clemson University
NA
Wednesday September 25, 2024 3:15pm - 3:30pm HST
Nautilus

3:15pm HST

PROP - Evaluation of Auxin Application and the Presence or Absence of Roots or Inflorescences in the Propagation of Variegated Carex wahuensis subsp. wahuensis
Wednesday September 25, 2024 3:15pm - 3:30pm HST
Carex wahuensis subsp. wahuensis is an endemic Hawaiian species extensively used as a ground cover in landscaping and restoration. In recent years, a variegated form was identified and is now starting to be utilized in a handful of landscaping projects. While the green wild-type has been primarily propagated through seeds, protocols for single plant divisions are lacking and limits the commercialization and availability of the variegated form. In this study, we investigated the effect of indole-3-butyric acid (IBA) application as well as the presence or absence of roots and inflorescences on propagating single plant divisions of variegated C. wahuensis subsp. wahuensis. Container grown clumps were divided into single plantlets, with or without flowers and with or without roots (trimmed 7.6 to 10.2 cm length). For the first experiment, plantlets without flowers but with pre-existing roots were treated with or without 3000 ppm IBA and planted in 1:1 perlite and vermiculite. For the second experiment, plants with roots but with or without flowers, and plants without roots and flowers were also planted in 1:1 perlite and vermiculite. Both experiments were located on a mist bench, which activated every 2 minutes for 12 seconds, and both were allowed to root for up to 135 days. Root length and rooting index was recorded for the first experiment at 45 and 90 days after planting while rooting index was recorded for the second experiment at 45, 90 and 135 days after planting. Application of IBA did not improve root length and rooting index of plantlets. However, there was an observed difference in root length and rooting index between experimental runs. In the second experiment, plants with roots and no flowers exhibited the highest rooting index (3.3 = light rooting) followed by plants with no flowers and no roots (2.9 = alive but no roots to light rooting). Plants with flowers and roots exhibited the lowest rooting index (1.7 = mostly dead). Results of the study indicate that vegetative single plantlets of variegated Carex wahuensis subsp. wahuensis can be successfully rooted within 135 days after planting under mist.
Speakers
PM

Paulo Matos

Teaching Assistant, University of Hawai'i
Co-authors
OB

Orville Baldos

University of Hawaii
Wednesday September 25, 2024 3:15pm - 3:30pm HST
Lehua Suite

3:25pm HST

ORGH/BioECP - Interactions between photoselective plastics and insect pest dynamics in an organic brassica vegetable system
Wednesday September 25, 2024 3:25pm - 3:35pm HST
Brassica crops are susceptible to a variety of insect pests of particular significance in organic production systems, including flea beetles, aphids, and lepidopteran caterpillar pests. These pests are abundant on brassicaceous vegetable crops throughout the U.S. and populations frequently are high enough to significantly reduce crop quality. There is an increased interest among small and mid-size vegetable growers to adopt poly-covered tunnels to protect high value horticultural crops. Tunnel production allows for season extension, can protect crops from adverse weather conditions, improve the quality of horticultural crops, and may also influence plant-insect dynamics. Photoselective poly coverings influence light wavelength and transmission in tunnel environments and may affect plant physiological responses as well as incidence of disease and herbivory by insect pests. Here, we explored the impact of UV light blocking and UV light transmitting poly coverings on Lacinato kale (Brassica oleracea var. acephala) production and the effects on pest and beneficial insect populations in a tunnel environment compared to an open field system on certified organic land in Morris, MN. Our results showed that total kale harvestable yield was similar between the poly covered tunnels and open field plot treatments. No differences in yield or insect pest pressure were observed between UV light blocking or transmitting poly covered tunnels but were observed between tunnels and the open field environment. Imported cabbageworm populations were 75% higher in tunnels versus open field plots (P=.0023), however the inverse was observed for cabbage loopers, which were 60% higher in open field plots (P=.0072). No flea beetles were found in tunnels, and an average of 1 beetle/plant was observed in open field plots. Diamond back moth and aphid populations were not different between the tunnel and the open field plots. Overall, insect pest populations did not reach economically damaging thresholds during the study period, likely due to the field plot location which did not have a history of brassica production. Our results showed that light transmission and ambient air temperatures were similar under the photoselective poly coverings. We observed that pest pressure increases rapidly in tunnel environments as the season progressed regardless of poly covering. Although our study did not observe differences in the yield or quality of kale across production systems, there were differences in insect pest populations across systems, indicating a different management approach may be needed to prevent crop damage in tunnel environments.
Speakers
avatar for Dilip Nandwani

Dilip Nandwani

TN State University
NA
Co-authors
AF

Aimee Foster

University of Minnesota
NA
LW

Leah Worth

University of Minnesota
NA
MR

Mary Rogers

University of Minnesota
Wednesday September 25, 2024 3:25pm - 3:35pm HST
South Pacific 4

3:30pm HST

CE 3 - Advantages of a novel in situ pH measurement for soilless media
Wednesday September 25, 2024 3:30pm - 3:45pm HST
Rhizosphere pH determines nutrient bioavailability, but this pH is difficult to measure. Standard pH tests require adding water to growth media. This dilutes hydrogen ion activity and increases pH. We used a novel, in situ, pointed-tip electrode to estimate rhizosphere pH without dilution. Measurements from this electrode matched a research-grade pH meter in hydroponic nutrient solutions. We then compared measurements from this electrode to saturated paste and pour-through methods in peat moss, coconut coir, and pine bark. The pointed-tip electrode was unable to accurately measure pH in the highly-porous pine bark media. Adding deionized water to the other media at container capacity using the saturated paste method resulted in a pH that was 0.59 ± 0.30 units higher than the initial in situ measurement at the top of the container. This increase aligns with established solution chemistry principles. Measurements of pH using the pour-through method were 0.38 ± 0.24 pH units higher than in situ measurements at the bottom of the container. We conclude that in situ pH measurements are not subject to dilution and are thus more representative of the rhizosphere pH than the saturated paste and pour-through techniques.
Speakers Co-authors
BB

Bruce Bugbee

Utah State University
NA
NL

Noah Langenfeld

Utah State University
NA
RH

Royal Heins

Utah State University
NA
Wednesday September 25, 2024 3:30pm - 3:45pm HST
Coral 1

3:30pm HST

POM 2/HPRB/IPR - Common Mechanisms Controlling Fruit Shapes may be Mediated by Changes in Cell Wall Properties
Wednesday September 25, 2024 3:30pm - 3:45pm HST
Fruit shape variation is abundantly present in horticultural crops. This variation is critical to highlight the market class as well as the culinary purpose of the produce. Many of the underlying genes have been cloned in tomato, offering insights into the molecular mechanisms of morphological diversity. Specifically, members of the OFP, TRM and SUN family regulate produce shape variation in tomato and other crops, thereby highlighting the importance of these three families in regulating phenotypic diversity. Despite the knowledge of the genes, mechanistic insights into the function of members of these three gene families are lacking. Our research on the tomato genes OVATE and OFP20 has shown that changes in produce shapes are noticeable early in the development of the flower. Cell counts in ovaries at anthesis implied that changes in cell division patterning may underlie morphological diversity. However, gene expression studies showed that morphological changes were associated with cell wall processes and not with changes in cell division patterning.
Speakers
EV

Esther van der Knaap

University of Georgia
Co-authors
YW

Yanbing Wang

University of Georgia
NA
Wednesday September 25, 2024 3:30pm - 3:45pm HST
Nautilus

3:30pm HST

PROP - Evaluating the Effect of Plant Growth Hormone Types and Rates on Osmanthus spp. Propagation
Wednesday September 25, 2024 3:30pm - 3:45pm HST
Osmanthus is a genus of ornamental plants with valuable qualities such as pest resistance, evergreen foliage, and aromatic flowers. However, different species respond differently to growth hormones for propagation. This study aimed to evaluate the types and rates of plant growth hormones on different species of Osmanthus propagation. Six distinct Osmanthus species (Osmanthus heterophyllus ‘Kaori Hime’, Osmanthus armatus ‘Jim Porter’, Osmanthus x fortunei ‘Patty's Secret’, Osmanthus heterophyllus ‘Rotundifolius’, Osmanthus delavayi, and Osmanthus x fortunei ‘Fruitlandii’) were treated with Indole-3-butyric acid (IBA) powder, 500 mg/L and 2000 mg/L of Potassium Indole-3-butyric acid (K-IBA), 10% Kelpak (seaweed extract), and water dip (the control). The cuttings’ survival rates, rooting rates, callus rate and area, and root length were recorded. The results showed the O. heterophyllus ‘Kaori Hime’ exhibited the best survival and rooting rates, while the O. heterophyllus ‘Rotundifolius’ had the lowest survival rate, the O. armatus ‘Jim Porter’ had the highest callus quantity. Plants treated with IBA powder had significantly larger callus area than those seaweed treatments. In conclusion, O. x fortunei 'Fruitlandii' and O. heterophyllus 'Kaori Hime' exhibit superior performance in all six measurements compared to other plant species and are highly recommended. Conversely, O. delavayi and O. heterophyllus 'Rotundifolius' have displayed considerably weaker performance and are not recommended. Further research is necessary to determine the effectiveness of other propagation methods and treatments for Osmanthus.
Speakers
avatar for Yulong Chen

Yulong Chen

The University of Georgia
Co-authors
LC

Lilin Chen

The University of Georgia
NA
PY

Ping Yu

University of Georgia
Wednesday September 25, 2024 3:30pm - 3:45pm HST
Lehua Suite

3:30pm HST

Industry Networking Cocktail Event
Wednesday September 25, 2024 3:30pm - 4:30pm HST
Join our industry members for a social networking hour.
Wednesday September 25, 2024 3:30pm - 4:30pm HST
Coral 4 - ASHS Collaboration Center

3:35pm HST

ORGH/BioECP - Anaerobic Soil Disinfestation: A Non-chemical Approach to Managing Weeds and Nematodes in Sweetpotato
Wednesday September 25, 2024 3:35pm - 3:45pm HST
Anaerobic soil disinfestation (ASD) is a promising organic pest management strategy and is an alternative to chemical-led approaches that has shown potential to manage weeds and soil-borne pathogens in organic vegetable production systems. ASD is facilitated by incorporating carbon sources into the soil, tarping the soil with plastic mulch, and irrigating to the soil saturation point. To evaluate the impact of ASD on weed and nematode management in organic-grown sweetpotato, greenhouse studies were conducted at Clemson University, Clemson, South Carolina. Experiments were laid out in a randomized complete block design in 2-gallon microcosms with two carbon sources [ASD (soil amended with chicken manure + molasses as carbon source) and non-ASD (non-amended control)] in the main plot and twenty sweetpotato genotypes in subplots. Three-week-old seedlings of tomato (cv. Rutgers) were planted in each microcosm followed by inoculation with 10,000 eggs of the southern root-knot nematode (Meloidogyne incognita). ASD was initiated one month post inoculation to allow nematodes to complete one life cycle. At the time of the ASD initiation, each microcosm was also inoculated with weed seeds [yellow nutsedge (10 tubers) and carpet weed (100 seeds)]. ASD was conducted for three weeks, followed by the transplantation of sweetpotato slips after one week of ASD termination. Weed counts, abundance of nematode second stage juveniles (J2) in soil, and sweetpotato above ground biomass data were collected. Our results suggested that the microcosms receiving the carbon amendment spent the most time under anaerobic conditions (<200 mvh). ASD lowered weed cover percentage (78%), individual weed count of yellow nutsedge (75%), and carpet weed (70%) as compared to non-ASD. The soil abundance of nematode J2 varied among the sweetpotato lines, with the lowest number observed in sweetpotato cultivar Ruddy (23/100 cm3 of soil) under ASD treatment. However, commercial cultivar Beauregard supported the greatest population under non-amended treatment (163/100 cm3 of soil). ASD treatment had significantly higher above-ground biomass (9.1 g) of sweetpotato compared to the non-ASD controls (4.2 g). Results of this greenhouse studies will be validated under field conditions.
Speakers
SS

Simardeep Singh

Clemson University
Co-authors
CK

Churamani Khanal

Clemson University
NA
Wednesday September 25, 2024 3:35pm - 3:45pm HST
South Pacific 4

3:45pm HST

ORGH/BioECP - Potential Establishment of Entomopathogenic Fungi as an Endophyte in Citrus Trees Over Time
Wednesday September 25, 2024 3:45pm - 3:55pm HST
Entomopathogenic fungi offer an alternative strategy for citrus growers seeking environmentally friendly pest management solutions. This study aimed to assess the ability of a commercial strain of the fungus Beauveria bassiana to become endophytic in citrus plants after a single foliar application. A completely randomized block design, consisting of ‘Valencia’ sweet orange trees (Citrus × sinensis) grafted on ‘US-942’ (Citrus reticulata × Poncirus trifoliata) rootstock, was established under greenhouse conditions. Treatments comprised 6 replications of treated plants in two separate greenhouses (n = 16 per greenhouse), along with one control (water only). Treated plants were foliar sprayed asynchronously with the fungus at the beginning of each experimental trial per treatment. To assess endophytism over time, a series of cohorts were destructively sampled every two months. Spore deposition was assessed using cover slips post-spray application of all treatments. Additionally, detached leaves collected post-spray were imprinted on potato dextrose agar (PDA), amended with dodine and bactericides, to determine spore coverage of the leaf surface by counting colony forming units. To assess endophytism, sterile samples of plant organs (i.e., leaves, stems, and roots) were placed onto PDA-dodine plates to allow detection of phenotypic mycelia. Results from the first cohorts indicated successful application of the fungi on the citrus leaves, and endophytism was assessed after two months. Additionally, no statistically significant changes were recorded in terms of plant height, root, stem, and leaf biomass, as well as stem girth. This project contributed to a clearer understanding of the long-term endophytic persistence of commercially available entomopathogenic fungi and their effect on plant growth in citrus trees. The trial will continue in the field for additional screening.
Speakers
JP

Joseph Paoli

University Of Florida
I am a Master's student at the University of Florida based at the Indian River Research and Education Center (IRREC). I am a long-time Florida resident, having gone to Middle and High School in Central Florida before enrolling at the University of Florida. During my four years of... Read More →
Co-authors
ED

Edinson Diaz

University of Florida
NA
JF

John-Paul Fox

University of Florida
LC

Liliana Cano

University Of Florida
NA
LR

Lorenzo Rossi

University of Florida
MR

Mark Ritenour

University Of Florida
NA
PA

Pasco Avery

University of Florida
NA
TB

Terri Baker

Indian River State College and University of Florida
NA
Wednesday September 25, 2024 3:45pm - 3:55pm HST
South Pacific 4

3:45pm HST

POM 2/HPRB/IPR - Pomological Nomenclature: Recent Developments and Problems
Wednesday September 25, 2024 3:45pm - 4:00pm HST
In addition to botanical names, at least a dozen distinct categories of nomenclature are applied to plant cultivars, including various forms of cultivar denominations, breeders references, and trade names. Two sets of rules, the International Code of Nomenclature for Cultivated Plants (9th ed., 2016), and the Explanatory Notes on Variety Denominations Under the UPOV Convention (2022) provide current guidelines for plant cultivar nomenclature. In some instances UPOV and ICNCP rules differ, and stakeholders may wish to consider whether it would be feasible to seek harmonization, and the mechanisms by which that might be achieved. This session will trace a brief history of cultivar denomination rules for U.S. plant patents. The United States Patent and Trademark Office, which issues plant patents, does not provide detailed nomenclatural guidelines. In the past two decades a new model for plant nomenclature has prevailed, in which an alphanumeric code serves as the official cultivar denomination, and this is paired with a trademark, either registered or unregistered. The relationship between cultivar denominations and trade names can be complex and fluid. As co-editor of the Register of New Fruit and Nut Cultivars, the presenter professionally researches all new pomological cultivar denominations and trade names, to avoid publishing names that conflict with previous names or nomenclatural standards. The establishment of an official cultivar denomination has important practical consequences that are sometimes ignored by breeders and rights owners. When a cultivar has been granted a plant patent or plant breeders’ rights, the cultivar denomination recorded by the statutory plant registration authority that issues the grant becomes officially established (a “statutory epithet”), and cannot be casually changed or replaced by the rights owner. When such informal synonyms are used, they are best regarded as trade names, often as unregistered trademarks. Common mistakes and pitfalls in nomenclature are described.
Speakers
DK

David Karp

University of California, Riverside
Wednesday September 25, 2024 3:45pm - 4:00pm HST
Nautilus

3:45pm HST

Interest Group Session: Biotechnology of Woody Ornamental Plants, Interest Group Meeting, 2024 PB Awards
Wednesday September 25, 2024 3:45pm - 5:45pm HST
This year’s PB session will combine a keynote speaker session, business meeting and PB 2024 award ceremony together.
1. Keynote speaker: Hui Duan, USDA-ARS-REE (45 minutes) Biotechnology of Woody Ornamental Plants
2. PB business meeting (30 minutes) Chair annual report, elect new chair, chair elected and secretory. Plan next year’s work.
3. 2024 Plant Biotechnology Interest Group annual award ceremony (45 minutes)
Moderator
KD

Kedong Da

North Carolina State University
Director of plant transformation lab at North Carolina State University
Speakers
Wednesday September 25, 2024 3:45pm - 5:45pm HST
Kahili

3:55pm HST

ORGH/BioECP - Impact of Biosolid Compost on Basil Plant Growth and Physiology
Wednesday September 25, 2024 3:55pm - 4:05pm HST
Biosolids, derived from treated sewage sludge, represent a significant resource in agricultural production, with approximately 70 percent being utilized for land application in the United States. Recognized for their potential as organic fertilizers, biosolids undergo meticulous processing to meet grade AA standards before being blended with locally sourced organic materials, such as woody yard debris, in preparation for municipal distribution.
This study delves into the effects of biosolids on plant growth rates using various concentrations ranging from 0% to 30%. Findings revealed that different concentrations of biosolids in soil significantly delay germination by up to two days. Through replicated mesocosm experiments at the Southwest Florida Research and Extension Center (SWFREC), UF/IFAS, Immokalee, we also evaluated the effects of biosolids on basil (Ocimum bacilicum) growth and physiology. Basil was cultivated in half-gallon pots containing various soil amendments of biosolids compost in concentrations of 0%, 5%, 10%, 15% and 30% over a 60-day period.
Comprehensive data collection includes measurements of plant height, leaf chlorophyll content, photosynthetic gas exchange, Normalized difference vegetation index (NDVI), fresh and dry plant biomass, and visual ratings. Data analysis of weekly and post-harvest collected data analyzes the implications of biosolid usage in agriculture, shedding light on significant effects on crop productivity, and agricultural sustainability. The treatments with higher biosolid compost concentrations showed an increase in plant productivity, specifically, the fresh biomass of the 30% treatment was 45% more than in the control.
Keywords: Biosolids, Organic Fertilizer, Compost, Soil Contaminants, Basil (Ocimum bacilicum), Plant Physiology, Agricultural Systems.
Speakers
MT

MADELEINE TENNY

Florida Gulf Coast University
Co-authors
JG

John Griffis

Florida Gulf Coast University
NA
Wednesday September 25, 2024 3:55pm - 4:05pm HST
South Pacific 4

3:59pm HST

Ornamental Plant Breeding 2 (OPB 2)
Wednesday September 25, 2024 3:59pm - 5:00pm HST
QTL and Transcriptomic Analysis of Fragrance in the Auto-tetraploid Rose Population - Haramrit Gill
Assessing Micropropagation Traits in Anthurium Towards Breeding and Cultivar Development - Jaclyn Nicole Uy
Genotype Comparisons of Anthurium In Vitro Shoot Production in RITA® Bioreactors - Jacob Olarti
Treatment with Oryzalin Induces Chromosomal Changes in Salvia coccinea and Salvia splendens - John Ruter
Moderator
TA

Teresita Amore

University of Hawaii
Wednesday September 25, 2024 3:59pm - 5:00pm HST
South Pacific 2

3:59pm HST

Weed Control and Pest Management 2/Invasive Plants Research (WCPM 2/IPR)
Wednesday September 25, 2024 3:59pm - 5:30pm HST
Planting-hole Steam Application for Pathogen and Weed Control in Organic Strawberry in Southern California - Oleg Daugovish
Elucidating the Impact of Anaerobic Soil Disinfestation on Organic Watermelon Production in South Carolina - Sohaib Chattha
Alternative Carbon Sources for Anaerobic Soil Disinfestation in California Strawberry - Oleg Daugovish
Sweetpotato (Ipomoea batatas) Variety Tolerance to Different Herbicidal Weed Control Methods - Alyssa Miller
Assessment of Various Carbon Sources for Anaerobic Soil Disinfestation to Manage Weeds in Organic Sweetpotato - Simardeep Singh
Evaluation of saturated steam with boiling water to control Guinea grass (Megathyrus maximus) in riparian
Moderator
avatar for Hannah Lutgen

Hannah Lutgen

Extension Faculty, University of Hawaii at Manoa, College of Tropical Agriculture & Human Resources (CTAHR)
As an Extension Agent faculty member at the University of Hawaiʻi at Mānoa College of Tropical Agriculture and Human Resources (CTAHR), I analyze issues, create programs, and conduct activities that meet client needs to support landscape professionals, ornamental and cut flower... Read More →
Wednesday September 25, 2024 3:59pm - 5:30pm HST
South Pacific 3

4:00pm HST

OPB 2 - QTL and Transcriptomic Analysis of Fragrance in the Auto-tetraploid Rose Population
Wednesday September 25, 2024 4:00pm - 4:15pm HST
For centuries, roses have been treasured for their therapeutic, cosmetic, and ornamental qualities. Among its many qualities, flower fragrance holds significant economic value. Over 400 volatile compounds contribute to the complex aroma of roses, with terpenoids, phenylpropanoids, and benzenoids playing dominant roles. Among these, geraniol, a monoterpene, contributes notably to the signature scent of rose oil. However, the fragrance of modern roses has gradually diminished as breeders have focused on enhancing other traits like appearance, adaptation, durability, and vase life. To gain a better molecular understanding of specialized metabolic pathways related to floral scent in roses we carried out QTL studies in the SWxBE autotetraploid rose population [Rosa L. ‘ORAfantanov’ (Stormy Weather™) x Rosa L. ‘Radbrite’ (Brite Eyes™)]. Our study suggests that the QTL for the fragrance was identified on chromosome 2 which colocalized with the genes involved with fragrance such as ODO1, EOBIII, and NUDIX. Two rose genotypes from the SWxBE population, ‘16401-N055’ (slightly fragrant) and ‘16089-N051’ (highly fragrant), were used for transcriptomic analysis. Gene expression analysis suggests that the monoterpenoid pathway was highly active in the highly fragrant rose with the NUDIX gene being highly expressed. Thus, NUDIX, a gene involved in geraniol biosynthesis, is a strong candidate gene for the QTL on chromosome 2. This study lays the groundwork for further exploration of the molecular pathways responsible for the scent of roses.
Speakers
HG

Haramrit Gill

University of California, Davis
NA
Co-authors
DB

David Byrne

Texas A
NA
JL

Jeekin Lau

US Department of Agriculture
NA
Wednesday September 25, 2024 4:00pm - 4:15pm HST
South Pacific 2

4:00pm HST

WCPM 2/IPR - Planting-hole Steam Application for Pathogen and Weed Control in Organic Strawberry in Southern California
Wednesday September 25, 2024 4:00pm - 4:15pm HST
Organic strawberry production has been expanding in California, but opportunities for crop rotation are limited due to lack of organically certified fields. Continuous strawberry production promotes soil-borne pathogens, such as Macrophomina phaseolina, the causal agent of charcoal rot and increases populations of weeds. These problems are exacerbated by lack of cost-effective management tools. In summer and fall production seasons at Oxnard, CA we evaluated pre-plant steam injection to raise soil temperature to 70 C or above for at least two minutes. Steam generated on-site was applied via four 25-cm long spikes to planting holes in raised beds covered with the plastic mulch. Five to ten days later, bare-root strawberry plants were placed in six steamed and six untreated plots and their performance and fruit production assessed. Additionally, we collected soil at 0-25 cm for analyses of resident Macrophomina phaseolina abundance before and after treatment and evaluated weed densities in planting holes. Steam application reduced M. phaseolina microsclerotia levels 80% in summer and 96% in fall. Steaming provided near 100% control of weeds germinated from the soil seed bank, but had no effect on germination of wind-dispersed weeds deposited to holes after steaming as was the case with fumigants. In summer season, strawberry mortality due to soil-borne pathogens was 12-18% in steamed plots and 70-75% in untreated soil. Due to loss of plants, marketable fruit yields in untreated plots were reduced 95% compared to steam treatment. No early-season plant mortality occurred in fall season but strawberry plants were 39 and 54% larger at two evaluation dates in steamed plots compared to untreated soil. We continue evaluations of fruit production and plant responses to soil pathogens. The completed work suggests that hole steaming may be very effective in suppressing soil-borne pathogens and weeds interfering with organic strawberry production in coastal California.
Speakers
OD

Oleg Daugovish

University of California Cooperative Extension
Co-authors
JB

Jenny Broome

UC Davis
NA
KI

Kelly Ivors

Driscolls
NA
OB

Oddbjorn Bergem

SoilSteam
NA
PH

Peter Henry

USDA-ARS
NA
Wednesday September 25, 2024 4:00pm - 4:15pm HST
South Pacific 3

4:00pm HST

Early Career Competition
Wednesday September 25, 2024 4:00pm - 6:00pm HST
Dr. Emran Ali, a faculty member and state horticulture specialist at Alcon State University, MS, leads cutting-edge research on horticultural crops.
Title: Tech-Powered Mobile Plant Clinics: Transforming On-Site Plant Disease Diagnosis in Extension Program

Dr. Michael Hardigan has worked as the Research Geneticist and Caneberry Breeder leading the USDA-ARS and Oregon State University cooperative small fruit breeding program since 2021.
Title: Supporting U.S. Caneberry Production With Genomic Breeding and Mechanization

Dr. Qingwu Meng is an Assistant Professor of Controlled-Environment Horticulture at the University of Delaware.
Title: To Infinity and Beyond: Advancing Research and Teaching in Controlled-Environment Horticulture

Dr. Sushan Ru, is an Assistant Professor and Small Fruit Breeder in the Department of Horticulture, Auburn University.
Title: Establishing the first small fruit breeding program in Alabama

Dr. Muhammad Shahid is a plant physiologist specializing in stress physiology of specialty crops with special focus on fruit crops with the University of Florida, Horticultural Sciences Department.
Title: Research-Driven Extension for Sustainable and Profitable Fruit Production in Florda under Climate Change Scenario
Moderator
avatar for Becky Sideman

Becky Sideman

Member-At-Large
Speakers
EA

Emran Ali

Alcorn State University
QM

Qingwu Meng

University of Delaware
SR

Sushan Ru

Auburn University
MS

Muhammad Shahid

University of Florida
Wednesday September 25, 2024 4:00pm - 6:00pm HST
Hibiscus

4:00pm HST

AI Innovation for Horticulture - Part 2
Wednesday September 25, 2024 4:00pm - 6:00pm HST
Introduction and Overview

Speaker: Kathryn Orvis
Professor
Department of Horticulture and Landscape Architecture
Purdue University
625 Ag Mall Drive
West Lafayette, IN 47907-2010

Title: Digital Agriculture and AI on Specialty Crops Production

Description: Digital agriculture is the 4th agricultural revolution and Artificial Intelligence (AI) is part of it. Currently, in the "connected agriculture"; era, many technologies have been released on the marked regarding the use of multispectral
sensors for many purposes in agriculture. This talk is going to cover information on how to use Digital Agriculture online platforms to process multispectral imagery, and how AI can be used to collect individual in-field plant data.

Speaker: Luan Pereira de Oliveira
Assistant Professor and Precision Agriculture Extension Specialist
Department of Horticulture
University of Georgia
139 Engineering Building
2329 Rainwater Road
Tifton, GA 31793

Title: Bringing the Future of AI to the Farm.
Description: In this talk, we will cover the multitude of use cases where AI can be applied in farming – from weed detection and robotics to Generative AI-based farm assistants and Virtual Reality. We go through the industry trends of applied Artificial Intelligence and think big about farm automation for the future.

Speaker: Justin Hoffman
Chief Technology Officer of AgTechLogic


Title: From Concept to Impact: The Evolution of Moss Robotics through Industry-
University Collaboration


Description: Moss Robotics' journey began with a project focused on autonomous driving technology for tree nurseries, born out of a collaboration between Carnegie Mellon University, Robotics Institute and Hale; Hine Nursery in Tennessee. In this talk, we share the story of how we discovered the real value our solution could offer to growers, and how we refined our ideas through continuous iteration. This process transformed moss robotics from a simple concept into the company it is today. We will cover the steps of our evolution, emphasizing the practical benefits of combining academic research with industry needs to innovate effectively. Additionally, we look ahead to how emerging technologies might further influence our growth and the agricultural industry as a whole, aiming for advancements in farming practices that are both technologically sophisticated and grounded in real-world applications.

Speaker: Di Hu
Founder and CEO
Moss Robotics

Title: AI-Enhanced Computer Vision for Crop Monitoring in Controlled Environment
Agriculture


Description: Controlled environment agriculture (CEA) production remains expensive due to high operation costs. Growers can reduce production costs by nurturing crops with data, however, the data is highly diverse, and growers lack the expertise to analyze this data to derive actionable insights for informed decision-making. In addition, traditional crop monitoring is carried out manually, which makes it unfeasible to collect data daily to get actionable insights for high yields. Recent advancements in sensing and computing technologies, such as AI, computer vision, edge computing, and edge-
cloud integration, have opened opportunities to develop data-driven technologies to enhance decision-making capabilities. Integrating AI and computer vision technologies has emerged as a transformative toolset that can collect real-time plant data at high spatial and temporal resolutions, pivotal in optimizing resource management and maximizing production. The CE Engineering lab delves into cutting-edge computer vision applications within CEA, focusing on various applications, including phenotyping leafy greens, yield estimation, disease monitoring, and plant spacing optimization. This presentation will explore the details of lettuce phenotyping, disease classification, strawberry fruit classification, and yield estimation. We will delve into the technical aspects of these algorithms, including image processing techniques, machine learning models, and data integration strategies. This presentation will showcase state-of-the-art deep learning approaches, including segmentation algorithms, model training, and deep classifiers. Overall, this presentation aims to provide insights into the transformative potential of computer vision in CEA, offering a glimpse into the future of data-driven and sustainable CE production.

Speaker: Azlan Zahid
Assistant Professor,
Department of Biological and Agricultural Engineering
Texas A&M AgriLife Research
Texas A&M University System
Dallas, TX 75252, USA


Panel: 30-minute panel with the above speakers, to allow time for Q&A and discussion.
Moderator Speakers
KD

Kent D. Kobayashi

Interim Dept. Chair, TPSS Dept., Univ. of Hawaii at Manoa
KO

Kathryn Orvis

Professor, Purdue Univ
avatar for Di Hu

Di Hu

CEO, moss robotics inc.
avatar for Justin Hoffman

Justin Hoffman

Chief Technology Officer, AgTechLogic
Wednesday September 25, 2024 4:00pm - 6:00pm HST
Coral 3

4:00pm HST

Interest Group Session: Choosing the Right Career Path-Research, Extension, Teaching, and Administration
Wednesday September 25, 2024 4:00pm - 6:00pm HST
The agricultural production system and the education system in Indian subcontinent and US are very different. Thus, students and post docs coming to US to pursue research and career encounter dilemma and challenges in selecting right career path. For example, in Indian subcontinent higher emphasis is given to research and teaching whereas extension and administration are not fully understood. The goal of this special session will be for students to have open dialogues with experts in the field of research, extension, teaching, and administration with Indian sub-continent background, so the panel understands the challenges faced by incoming students. This session will benefit all the graduate students and researchers who are preparing to for the next step in career.

Coordinator(s)
  • Tripti Vashisth, University of Florida, Lake Alfred, United States
Speaker/Participant(s)
  • Bhimu Patil, Texas A&M University, Vegetable and Fruit Improvement Center, College Station, Texas, United States
  • Ajay Nair, Iowa State University, Ames, Iowa, United States
  • Rajasekaran Lada, Dalhousie University, Truro, Nova Scotia, Canada

Moderator
TV

Tripti Vashisth

University of Florida
Speakers
Wednesday September 25, 2024 4:00pm - 6:00pm HST
South Pacific 1

4:00pm HST

Interest Group Session: Using Applied Economics to Explore the Many Facets of Adding Value to the Horticultural Industry
Wednesday September 25, 2024 4:00pm - 6:00pm HST
The business environment in the horticultural industry is evolving and consumer preferences for value-added products are shaping market trends. With an increasing emphasis on health, sustainability, and convenience, consumers are seeking horticultural products that offer added value and benefits. This shift in consumer preferences presents both challenges and opportunities for horticultural businesses. Understanding these evolving consumer demands is crucial for horticulture businesses to develop innovative products and marketing strategies that resonate with their target audience.

The session aims to explore consumer behavior and preferences for value-added horticultural products, providing attendees with valuable insights to inform strategic decision-making and drive business growth in the competitive market. Presentations will explore the specific information consumers seek on wine packages and labels, and the influence of state marketing programs on consumer preferences for tomato transplants. Additionally, the session will explore the return on investment of social media marketing for US green industry firms, consumer preferences and willingness to pay for Florida-grown fresh blueberries, strategies for expanding marketing potential to appeal to all horticulture consumers, and the influence of demographics on the perception of flatheaded borer damage on ornamental plants. Through these diverse presentations, attendees will gain insights into key factors shaping consumer behavior, industry dynamics, and marketing strategies in the horticulture sector.

Coordinator(s)
  • Alicia L Rihn, University of Tennessee, Knoxville, United States
  • Melinda Knuth, North Carolina State University, Horticultural Science, Raleigh, North Carolina, United States
  • Chengyan Yue, University of Minnesota-Twin Cities (Minneapolis, MN), St. Paul, Minnesota, United States
Moderator(s)
  • Alicia L Rihn, University of Tennessee, Knoxville, United States
  • Melinda Knuth, North Carolina State University, Horticultural Science, Raleigh, North Carolina, United States
Speaker/Participant(s)
  • Alicia L Rihn, University of Tennessee, Knoxville, United States
    Do State Marketing Programs Impact Consumer Preferences for Tomato Transplants? (15 mins)
  • Ariana P Torres, Purdue University, United States
    The Return of Investment of Social Media Marketing: A Case of U.S. Green Industry Firms (15 mins)
  • Chengyan Yue, University of Minnesota-Twin Cities (Minneapolis, MN), St. Paul, Minnesota, United States
    What Information Consumers Are Looking for On Wine Packages and Labels? (15 mins)
  • Hayk Khachatryan, University of Florida, United States
    Analyzing Consumer Preferences and Willingness to Pay for Florida-Grown Fresh Blueberries (15 mins)
  • Melinda Knuth, North Carolina State University, Horticultural Science, Raleigh, North Carolina, United States
    Expanding Marketing Potential to Appeal to All Horticulture Consumers (15 mins)
  • Ben Campbell, University of Georgia, United States
    Demographic Influences on the Perception of Flatheaded Borer Damage on Ornamental Crops (15 mins)
    Summary: Mounata Dahal, Tate Norman, Ben Campbell, and Alicia Rihn
Moderator
MK

Melinda Knuth

North Carolina State University
Speakers
BC

Ben Campbell

University of Georgia
CY

Chengyan Yue

University of Minnesota-Twin Cities (Minneapolis, MN)
Wednesday September 25, 2024 4:00pm - 6:00pm HST
Coral 1

4:14pm HST

Growth Chambers and Controlled Environments 2 (CE 2)
Wednesday September 25, 2024 4:14pm - 5:35pm HST
Carbohydrate Synthesis and Freeze Tolerance of Clonal-type Bermudagrasses as Affected by Mowing Heights During Cold Acclimation and Deacclimation Process - Mingying Xiang
Understanding Photoperiod Effects on Seek and Seedling Quality in Soybean Speed Breeding- Christiane da Silva
Identifying the Optimal Range of pH Management for Hydroponic Leafy Vegetables - Yujin Park
Enhancing Iron Bioavailability in Hydroponic Leafy Greens by Iron Chelators - Asmita Nagila
Effects of Mycorrhiza and Ciochar Interactions on Geraniums - Teal Hendrickson
Exploring Different Nutrient Solution Quantities to Optimize Quantity and Quality in Hydroponic Production - Puja Subedi
How Does Supplementing Dissolved Oxygen Impact Hydroponic Strawberry Production? - Jonathan Ries
Exploring Different EC Levels to Optimize Quantity and Quality in Hydroponic Production - Puja Subedi

Moderator
avatar for Puja Subedi

Puja Subedi

Kansas State University
I am a graduate research assistant working at the Kansas University Olathe, 22201 W Innovation Dr, Olathe, KS 66061. My major is urban food system under the department of horticulture and natural resources. Currently, I am working in the hydroponics research project.
Wednesday September 25, 2024 4:14pm - 5:35pm HST
Coral 2

4:15pm HST

CE 2 -Carbohydrate Synthesis and Freeze Tolerance of Clonal-type Bermudagrasses as Affected by Mowing Heights During Cold Acclimation and Deacclimation Process
Wednesday September 25, 2024 4:15pm - 4:25pm HST
Bermudagrass (Cynodon spp.) is a prominent warm-season turfgrass extensively utilized across golf courses, athletic fields, home lawns, and recreational areas due to its excellent heat tolerance, good traffic tolerance, and strong recuperative potential. Despite its strengths, winter survivability in colder climates remains a concern. Research has revealed variations in freeze tolerance across bermudagrass cultivars, yet there's a gap in understanding the underlying physiological mechanisms and the impact of cold acclimation and deacclimation processes. Additionally, the influence of mowing height on carbohydrate synthesis and freeze tolerance has been reported. To address these gaps, a study utilizing turfgrass plugs from different acclimation stages was conducted in a freeze chamber. The objective of this study was to examine freeze tolerance and carbohydrate synthesis in four clonal-type bermudagrass varieties ('Tifway’, ‘Tahoma 31’, ‘Astro’, and ‘TifTuf’) under mowing heights of 0.5” and 1.5” across various cold acclimation and deacclimation stages. The plugs were subjected to soil temperatures ranging from -5°C to -12°C, with survival assessed after three weeks to determine the lethal temperature (LT50) for each condition. Rhizome carbohydrate levels at each stage were determined. The correlation between rhizome carbohydrate level and freeze tolerance was determined. The data from this study is currently being analyzed.
Speakers
MX

Mingying Xiang

Oklahoma State University
Co-authors
DM

Dennis Martin

Oklahoma State University
NA
LZ

Lu Zhang

Oklahoma State University
SY

Shuhao Yu

Oklahoma State University
YS

Yanwei Sun

Oklahoma State University
NA
Wednesday September 25, 2024 4:15pm - 4:25pm HST
Coral 2

4:15pm HST

OPB 2 - Assessing Micropropagation Traits in Anthurium Towards Breeding and Cultivar Development
Wednesday September 25, 2024 4:15pm - 4:30pm HST
The major bottleneck in anthurium cultivar release is the availability of microprogated plants for field testing. Genotype has been a major consideration for anthurium protocol development. Different genotypes vary in in vitro performance even when using optimized media, therefore assessing proliferative variation under in vitro conditions could help identify cultivars that could introgress tissue culture traits and provide guidelines for future protocol development. The objective of this study is to assess the in vitro performance of different anthurium accessions from the University of Hawaii anthurium breeding program under the RITA® temporary immersion system and to observe how lineage affects in vitro performance. To evaluate shoot initiation/proliferation, 20 accessions of anthurium (parents, interspecific hybrids, complex hybrids, and wild relatives) were placed in RITA® supplemented with a liquid medium containing 0.3X MS salts with 0.2 mg/L BA, 15% coconut water and 20 g/L sucrose. Primary shoots were excised after 45 days to allow axillary buds to develop into secondary shoots. Bud masses (trimmed explant bases) were placed on a solid medium containing ½ MS salts with 15% coconut water, 20 g/L sucrose and 2g/L gellan gum to observe shoot proliferation and growth. Parameters were analyzed using mixed models with time block as random effects and accessions as fixed effects. Previously identified check cultivars (‘Marian Seefurth’ and ‘New Pahoa Red’) were used as controls in this study. Significant differences among the genotypes were observed in terms of the number of primary shoots, the number of secondary shoots, total axillary bud mass volume (cm3), number of explants with shoot, and number of explants with roots. Three selections and an accession were identified to have potential use for breeding. UH2053, UH2409, UH2327 and ‘New Pahoa Red’ showed superior or comparable number of primary and secondary shoots, explant volume, and rooting compared to the check cultivars. These lines will be considered as parents in future crosses for cultivar development. Cluster analysis was also performed and was cross-referenced to existing pedigree and breeding records. Our analysis revealed five clusters which indicate that that parentage influenced in vitro shoot production particularly in lines with Anthurium andraeanum, A. amnicola, A. formosum and A. kamemotoanum in their background. Pedigree and breeding records are valuable resources for predicting response profiles of anthurium in vitro performance.
Speakers
JN

Jaclyn Nicole Uy

University of Hawaii
Co-authors
JO

Jacob Olarti

University of Hawaii
NA
TA

Teresita Amore

University of Hawaii
Wednesday September 25, 2024 4:15pm - 4:30pm HST
South Pacific 2

4:15pm HST

WCPM 2/IPR - Elucidating the Impact of Anaerobic Soil Disinfestation on Organic Watermelon Production in South Carolina
Wednesday September 25, 2024 4:15pm - 4:30pm HST
Weeds and soil-borne pathogens are limiting factors in organic watermelon (Citrullus lanatus) production. Yellow nutsedge (Cyperus esculentus) is a problematic weed for Southeastern watermelon growers. Fusarium wilt caused by Fusarium oxysporum f.sp. niveum (FON), is responsible for significant yield loss in watermelon production. Inefficient non-chemical tactics are an impediment to curtail weeds and soil-borne diseases in organic watermelon; and necessitate the adaption of an alternative strategic and holistic approach. Anaerobic soil disinfestation (ASD) has the potential to control weeds and soil-borne pathogens across a range of environments and crop production systems. ASD is a preplant chemical independent technique, which requires incorporation of labile organic carbon (C) sources into the soil, followed by tarping the soil with plastic mulch, and irrigating the soil to the saturation. Shifts in soil microbial communities and production of volatile organic compounds during ASD process are the main mechanisms that are believed to kill soil-borne pathogens and weeds seeds. The objectives of this study were to 1) determine whether ASD can suppress the emergence of yellow nutsedge, 2) compare the efficacy of locally available C sources, 3) evaluate if ASD can influence grafted and non-grafted watermelon yield. A field trial was conducted at Clemson University’s, Coastal Research and Education Center in Charleston, South Carolina in 2023. The study was arranged in a randomized complete block design with four replications. Main C sources were subjected to control (CT) with no C, chicken manure molasses (CMM), and cotton seed meal (CSM). All treatments were assigned as ASD with non-grafted (Powerhouse) and grafted (Carolina strongback) rootstock grafted to scion Powerhouse. Indicators of reduction in soils (IRIS) tubes paint removal (%), yellow nutsedge shoot count, and crop yield data were recorded. Higher anaerobic soil conditions and lower yellow nutsedge shoot count were observed in ASD plots. At the time of watermelon harvest, total number of yellow nutsedge shoot count were recorded as 65, 25, and 22 in CT, CSM, and CMM, respectively. Higher marketable fruit yield was recorded in ASD plots treated with CMM. Based on weed control and yield assessments, CMM to facilitate ASD is an ideal practice for growing organic watermelon in South Carolina.
Speakers
avatar for Sohaib Chattha

Sohaib Chattha

Graduate Research Assistant, Clemson University
Co-authors
BW

Brian Ward

Clemson University
MC

Matthew Cutulle

Clemson University
Wednesday September 25, 2024 4:15pm - 4:30pm HST
South Pacific 3

4:25pm HST

CE 2 - Understanding Photoperiod Effects on Seed and Seedling Quality in Soybean Speed Breeding
Wednesday September 25, 2024 4:25pm - 4:35pm HST
Soybean is a short-day plant, which means that days must be shorter than a critical value to induce flowering. Manipulating the photoperiod regime is a well-known way to shorten plant cycles in speeding breeding programs. However, the impact of the photoperiod on the quality of the produced seeds is not well understood. Here, we investigate how photoperiod affected the seed and seedling quality in soybean plants, grown in a controlled environment. Soybean (Glycine max) plants (var. S16-14801C and CZ7570LL) were grown in growth chambers with controlled temperature (27 ± 0.5˚C), CO2 (475 ± 15 µmol mol-1), humidity (70 ± 5.0%), and light (300 ± 5 µmol m-2 s-1 at table; 20% blue,10% green, 70% red). One week after germination, seedlings were exposed to different photoperiod regimes: i) 10 h (0 w at 18 h), ii) two weeks at 18 h and then 10 h (2 w at 18 h), iii) four weeks at 18 h and then 10 h (4 w at 18 h); iv) six weeks at 18 h and then 10 h (6 w at 18 h). The plants were grown in the described treatments until the R8 stage (95% brown pods), without changing the light fixture height (industry standard practice). A sample of seeds was harvested and analyzed regarding quality while other samples were placed to germinate in seed germination paper to evaluate germination rate and seedling growth for 10 days. Similar results were found for both varieties; plants of all treatments presented different heights, in which plants at 0 w at 18 h were shorter (50 cm) and 6 w at 18 h taller (180 cm). Treatments did not affect the moisture or weight of 100 seeds. Conversely, germination and seedling survival were 30% lower in seeds from plants 0 w at 18 h than in other treatments. Similar results were found for the root (13% lower in 0 w at 18 h) and shoot length (19% lower in 0 w at 18 h) of seedlings. However, the dry weight of seedlings was similar among treatments. Manipulating the photoperiod can speed up the plant cycle and is a good alternative for speed-breading programs. However, extreme photoperiods and low daily light integral can produce seeds and seedlings with lower quality that can influence the production of plants of the next generation.
Speakers
avatar for Cristiane da Silva

Cristiane da Silva

Postdoctoral Research Scholar, North Carolina State University
I'm Cristiane, a plant physiologist with experience in plant biochemistry and plant responses to abiotic stress. Currently, I'm a post-doc at NCSU studying the effects of light on plant life cycles to expedite breeding purposes in crop and tree species. When I'm not working, I enjoy... Read More →
Co-authors
CC

Cristian Collado

North Carolina State University
NA
RH

Ricardo Hernandez

North Carolina State University
NA
Wednesday September 25, 2024 4:25pm - 4:35pm HST
Coral 2

4:29pm HST

Commercial Horticulture/ Federal Partners (CHEX/FP)
Wednesday September 25, 2024 4:29pm - 5:10pm HST
Advances in Phytophthora, Pythium, and Phytopythium Species Detection, Isolation, Culture, Diagnostics, and Virulence Rejuvenation -William Errickson
Effect of Planting Date on Strawberry Yield - Samantha Simard
Evaluation of Irrigation Strategies in Watermelons - Juan Enciso
Moderator
WE

William Errickson

Rutgers University
Wednesday September 25, 2024 4:29pm - 5:10pm HST
Lehua Suite

4:30pm HST

CHEX/FP - Evaluation of Irrigation Strategies in Watermelons
Wednesday September 25, 2024 4:30pm - 4:40pm HST
The Lower Rio Grande Valley farmers traditionally irrigate vegetable crops with furrow irrigation systems. To conserve water and mitigate the effects of droughts, there is a need to adopt more efficient irrigation and fertilization methods and practices in vegetable crops to increase crop production quality and profitability. Farmers that use furrow irrigation systems apply from 4 to 6 inches per irrigation depending on their furrow length and apply more than five irrigations during the vegetable growing season, and using between 20 inches (1.7 ac-ft) and 40 inches (3.3 ac-ft) of irrigation water in their fields. Vegetables have a shallow root system, so farmers must irrigate frequently to maintain good moisture levels during the growing season for optimum growth, possibly wasting water. We use image analysis derived from Unmanned Aerial Systems (UAS) and irrigation soil water sensors to provide management recommendations to schedule drip and surface irrigation to conserve additional amounts of water. We established replicated research experiments using subsurface drip irrigation and soil water sensors (watermark sensors) to irrigate watermelons and other vegetable crops. We grew plants under three different water levels to trigger irrigation (50, 75, and 100 cb). A drip irrigation system with plastic mulch was placed in the field, as well as soil-water sensors, to measure and monitor the soil moisture. After calculating the water used in the three water level treatments, the 50 cb treatment used 0.27 ac-ft, the 75 cb treatment used 0.24 cb and 0.22 ac-ft was used by the 100 cb treatment. According to our results, we could conserve up to 3.0 ac-ft with our recommendations. We concluded that watermelons could be managed when the soil-water sensor readings range between 50 and 75 CB and approximately 0.3 ac-ft of water using subsurface drip irrigation. We obtained an average yield of 53,536 lb/ac, when irrigated under the 50 cb treatment, 42,059 lb/ac at 75 cb, and 36,719 lb/ac at 100 cb.
Speakers Co-authors
Wednesday September 25, 2024 4:30pm - 4:40pm HST
Lehua Suite

4:30pm HST

OPB 2 - Genotype Comparisons of Anthurium In Vitro Shoot Production in RITA® Bioreactors
Wednesday September 25, 2024 4:30pm - 4:45pm HST
Anthuriums are Hawaii’s top cut flower with its sales valued at USD1.6 M in 2022. It is an important floriculture crop as it is both used as cut flower and foliage. Development of new genotypes allows the growers in Hawaii to produce new cultivars desired by the market. The University of Hawaii anthurium breeding program has identified new selections to field test with commercial growers. In order to do so, the breeding program propagates these selections to send to growers. Recently the use of bioreactors, such as RITA® on ‘New Pahoa Red’, resulted in three times more shoot production than in traditional flasks placed on a platform shaker. Shoot and root production and axillary bud mass volume of three new selections, UH2647, UH2651, and UH2652, were compared against the control ‘New Pahoa Red’. Ten nodes of each genotype were placed in a RITA® bioreactor, and replicated five times in a completely randomized design. Nodes were cultured in liquid medium consisting of 0.3MS, 0.1 mg l-1 BA, 15% coconut water, 20 g l-1 sucrose, and 1 ml l-1 NaSiO3. The plants were cultured in the bioreactors for 1.5 months, and then removed to obtain the number of shoots, roots, and axillary bud mass volume. Primary shoots were excised and transferred to 0.3 MS, 15% coconut water, 20 g l-1 sucrose, and solidified with 6 g l-1 Gelzan® to promote axillary bud growth and shoot development. The pH for all media was adjusted to 5.8. One month later, shoots (~0.5 cm in height) were excised from the axillary bud masses. Shoot counts excised after initial culture in the liquid medium and after placement in the shoot development solid medium were analyzed using one-way ANOVA on R studio. Axillary bud mass volume and root number were analyzed. UH2647 significantly produced more shoots than UH2652 and ‘New Pahoa Red’, while UH2651 was not different from any genotype. UH2647 also had the highest average axillary bud mass volume. UH2651 and UH2652 produced significantly more roots than the control. Knowing the genotypic differences in shoot production will assist propagation facilities in developing guidelines to schedule production of individual genotypes.
Speakers
JO

Jacob Olarti

University of Hawaii
NA
Co-authors
JN

Jaclyn Nicole Uy

University of Hawaii at Manoa
TA

Teresita Amore

University of Hawaii
Wednesday September 25, 2024 4:30pm - 4:45pm HST
South Pacific 2

4:30pm HST

WCPM 2/IPR - Alternative Carbon Sources for Anaerobic Soil Disinfestation in California Strawberry
Wednesday September 25, 2024 4:30pm - 4:45pm HST
Anaerobic soil disinfestation (ASD) has been adopted in approximately1,000 ha in California strawberry production as an alternative to chemical fumigation of soil. Rice bran, the predominant carbon source for ASD, has become increasingly expensive. In 2022-2024 field studies at Santa Paula and Oxnard, CA we evaluated 20-30% lower-priced wheat middlings (Midds) at 6 or 7 t/acre as alternative carbon sources to rice bran. The ASD treatments were applied in August at each location in preparation for strawberry planting in October. Soil and air temperatures were 18-35 C during that time. After incorporation of carbon sources into the top 30 cm of bed soil, beds were shaped, irrigation drip lines installed and covered with totally impermeable film (TIF) to prevent gas exchange. Beds were irrigated to full capacity within 24 to 72 hours after TIF installation. Anaerobic conditions were measured with oxidation reduction potential (ORP) sensors placed at 15 cm depth. Midds plots maintained Eh at -180 to 0 mV during the two ASD weeks at Santa Paula and -300 to 0 mV during five weeks at Oxnard, while untreated soil was aerobic at 200 to 400 mV. At Santa Paula, permeable bags with inoculum of Macrophomina phaseolina, a key soil borne pathogen of strawberry, and tubers of Cyperus esculentus, the most difficult to control weed, were placed 15 cm deep in soil and retrieved two weeks after ASD initiation for analyses. At Oxnard, resident populations of M. phaseolina and C. esculentus in soil were assessed before and after ASD. Two weeks after the completion of ASD, holes were cut to aerate beds and bare-root strawberry were transplanted into them: ‘Fronteras’ at Santa Paula and ‘Gaviota’ at Oxnard. ASD with Midds reduced viable microsclerotia of M. phaseolina 75% at Santa Paula and 98% at Oxnard. ASD treatments reduced tuber germination of C. esculentus 68-74% compared to untreated soil. Additionally, Midds and DDG provided greater sufficiency of plant-available nitrogen and increased fruit yields 40%, compared to untreated soil at Santa Paula. We continue fruit production evaluations at Oxnard in 2024. ASD with wheat middlings as a carbon source can suppress soil pathogens and weeds and help sustain organic strawberry production in California.
Speakers
OD

Oleg Daugovish

University of California Cooperative Extension
Co-authors
PH

Peter Henry

USDA-ARS
NA
Wednesday September 25, 2024 4:30pm - 4:45pm HST
South Pacific 3

4:35pm HST

CE 2 - Identifying the Optimal Range of pH Management for Hydroponic Leafy Vegetables
Wednesday September 25, 2024 4:35pm - 4:45pm HST
In hydroponics, the pH of the nutrient solution influences the solubility and availability of essential nutrients. The optimal pH for plant nutrient uptake in many crop species is around 6.0. However, the impacts of precise pH management on plant nutrient uptake, crop yield, and the optimal pH range remain less clear. In this study, we investigated the effects of pH management range on plant nutrient uptake and the growth of hydroponic leafy vegetables. Within an indoor vertical farm, we grew lettuce (Lactuca sativa) 'Rex,' kale (Brassica oleracea var. sabellica) 'Red Russian,' and arugula (Eruca sativa) 'Astro' using deep water culture hydroponics at the air temperature of 22 °C under a photosynthetic photon flux density of 200 μmol∙m−2∙s−1 with a 24-h photoperiod. The experiment included six pH treatments: pH 6, 6±0.5, 6±1.0, 6±1.5, 6±2.0, and without pH control. Compared to managing pH at 6, maintaining pH within 6±1.0 had generally similar impacts on leaf number, leaf area, SPAD index, shoot and root fresh mass, and shoot and root dry mass in all three crops. However, when compared to managing pH at 6, maintaining pH at 6±1.5 or greater reduced leaf area (by 32-47% in lettuce, by 30-41% in kale, or by 56-65% in arugula) and shoot fresh mass (by 33-54% in lettuce, by 37-45% in kale, or by 48-64% in arugula). Furthermore, in comparison to managing pH at 6, maintaining pH at 6±1.5 or greater also decreased leaf number in lettuce by 3-5 leaves and in arugula by 13-15 leaves but increased the root fresh mass of lettuce by 26-43%. Our results suggest that maintaining pH within 6±1.0 can be effective in promoting optimal nutrient uptake and overall plant development in the context of hydroponic cultivation.
Speakers
YP

Yujin Park

Arizona State University
Co-authors
ME

Matthew Easter

Arizona State University
NA
ZC

Zhihao Chen

Arizona State University
NA
Wednesday September 25, 2024 4:35pm - 4:45pm HST
Coral 2

4:40pm HST

CHEX/FP - Advances in Phytophthora, Pythium, and Phytopythium Species Detection, Isolation, Culture, Diagnostics, and Virulence Rejuvenation
Wednesday September 25, 2024 4:40pm - 4:50pm HST
Not only are Phytophthora, Pythium, and interesting Phytopythium species potentially devastating horticultural pathogens, they regularly present challenges in vitro. In this study, multiple established and novel methodologies were built upon to bolster researchers’ ability to quickly isolate, differentiate, and promote virulence of multiple oomycetes principally collected from symptomatic conifers and nearby water sources. These methods allow greater flexibility in generating clean mycelial cultures for genetic characterization, varying pathogen structures for use in novel bioassays (such as synchronized production of sporangia or zoospores), and ultimately inoculations to evaluate oomycide efficacy or make headway towards completion of Koch’s postulates for previously uncharacterized host: pathogen pairings. Phytopythium vexans (Pp. vexans) (n=8 isolates), three Phytophthora species / species complexes including P. cinnamomi (n=10), P. cryptogea / drechsleri complex (n=4) and P. humicola (n=2), as well as 5 tentative species of Pythium, were evaluated. Isolations took two forms, standard root sampling onto Phytophthora selective media (PARPH), or water-based sampling through a modified ‘baiting/trapping’ technique that utilized on-site collected water samples in the laboratory. The ‘baits’ were Cannabis sativa seed, Vigna radiata beans, and Rhododendron maximum leaves suspended in aerated water samples or slurry of silt/soil. Samples were evaluated on V8-agar (V8A), pea agar (PA), pea broth (PB), potato dextrose agar (PDA), cornmeal agar (CMA), and water agar (WA), each of which provided distinct morphological indicators and structures useful in diagnostic guides and in bioassays or inoculations. As is typical with all plant pathogens, the longer they remain in culture, the less virulent they may become. With oomycetes, this is compounded as the pathogen will often go into chlamydospore or oospore formation (long lived survival structures) which are not ideal for experimentation. Inclusion of germinated then surface sterilized (70% ethanol for 30s) Vigna radiata and Lupinus perennis sprouts into recently poured (still liquid) 1/8 clarified V8 juice agar (1/8 clV8A) provided a media capable of rejuvenating the pathogen due to presence of living roots and dynamic plant nutrients. This allows for more predictability of zoospore formation, especially if they are intended to be used with a time sensitive trials. In numerous incidences multiple species of Phytophthora and Phytopythium vexans isolates went into zoospore release simultaneously by utilizing these approaches in combination with resource starvation and culture washing with sterile distilled water. Taken together these approaches will greatly aid any researcher working with root disease oomycetes in culture.
Speakers
WE

William Errickson

Rutgers University
Wednesday September 25, 2024 4:40pm - 4:50pm HST
Lehua Suite

4:45pm HST

CE 2 - Enhancing Iron Bioavailability in Hydroponic Leafy Greens by Iron Chelators
Wednesday September 25, 2024 4:45pm - 4:55pm HST
Iron is an essential micronutrient for the growth and development of both plants and humans, as it plays vital roles in processes such as protein synthesis, respiration and DNA replication. Leafy greens, vital dietary sources of iron, can be cultivated with increased bioavailable iron through hydroponics by customizing nutrient solutions. Conventionally, iron chelates like EDTA and DTPA, are used in hydroponics, but challenges persist in iron acquisition due to their pH dependency as well as quick oxidation to ferric ion which is harder to uptake by plants. Good sources of chelates that respond well to high pH values, like EDDHA, are often more expensive. Studies suggest that iron complexed with humic substances exhibits higher efficiency, though confirmation in large-scale hydroponic systems is still needed. Fulvic acids are water-soluble humic substances with lower molecular weights that hold promise as alternatives or supplements to synthetic chelates, enhancing iron uptake and stress tolerance. Hydroponic systems, such as Deep-Water Culture (DWC) or Nutrient Film Technique (NFT), impact plant growth and nutrient uptake differently based on temperature, EC, and pH. This research compared the effects of various iron chelators on lettuce and kale cultivation in DWC and NFT systems. Results indicate significant yield loss in iron-deficient kale, while iron-chelated solutions enhanced yields. The addition of fulvic acid to EDTA-chelated solutions notably improved kale yield in DWC compared to no iron and EDTA-only solutions. Leafy greens showed higher chlorophyll fluorescence (Fv/Fm ratio) and chlorophyll content in DWC compared to NFT. The results showed species-specific and system-specific responses. Notably, iron-chelated plants exhibit higher iron content correlating with increased shoot weight and chlorophyll content. The effect of fulvic acids and synthetic chelates might be synergistic, with both providing different advantages that can be complementary in hydroponic solutions. This study highlights the importance of iron management in hydroponics and the way forward for iron fortification techniques.
Speakers
AN

ASMITA NAGILA

Graduate Research Assistant, Texas A&M university
Co-authors
Wednesday September 25, 2024 4:45pm - 4:55pm HST
Coral 2

4:45pm HST

OPB 2 - Treatment with Oryzalin Induces Chromosomal Changes in Salvia coccinea and Salvia splendens
Wednesday September 25, 2024 4:45pm - 5:00pm HST
Salvia coccinea (diploid) and Salvia splendens (tetraploid) and their associated cultivars are widely available, commercially profitable, and environmentally supportive flowering annuals. In recent years, exciting cultivars have been successfully introduced. This investigation explores further development of S. coccinea ‘Summer Jewel Lavender’, S. coccinea ‘Hummingbird Forest Fire’, and S. splendens ‘Mojave Red and White Bicolor’ through treatments by oryzalin for the potential development of novel cultivars. Surflan™ or oryzalin (4-(dipropylamino)-3,5-dinitrobenzenesulfonamide) and the nonionic surfactant SilEnergy™ were applied as a foliar spray for one, two, three, and six days to induce changes in chromosome numbers. Data collected included morphological observations, measurements of foliage and flowers, flower numbers, and ploidy analysis via a CytoFLEX™ flow cytometer. Ploidy changes were achieved in seedlings across all the treatment groups. Novel traits were identified, ranging from smaller overall growth habits and foliage, larger and increased numbers of flowers, and new floral pigmentation while maintaining vigor and potential for container production. Optimization of treatments will be discussed. The findings of this research have practical implications for plant breeders, landscape designers and architects, horticulturists, and environmental researchers as the market continues to demand drought-tolerant, low-growing, long-flowering, and pollinator-attracting plants.
Speakers
avatar for Brad Davis

Brad Davis

UGA College of Environment and Design
Co-authors
JR

John Ruter

University of Georgia
Wednesday September 25, 2024 4:45pm - 5:00pm HST
South Pacific 2

4:45pm HST

WCPM 2/IPR - Sweetpotato (Ipomoea batatas) Variety Tolerance to Different Herbicidal Weed Control Methods
Wednesday September 25, 2024 4:45pm - 5:00pm HST
There are limited herbicides labeled for use in sweetpotato (Ipomoea batatas) production in the United States. Therefore, the registration of additional herbicides with different modes of action (MOA) would provide growers added weed control options to enhanced crop yield and provide a more sustainable sweetpotato (SP) production system. As herbicide-resistant weed populations continue to emerge and become more prevalent, weed control strategies need to include herbicides with different MOAs for the long-term success of (SP) cultivation. This research will identify herbicides, along with rate and application time that could be registered for use in sweetpotato. Herbicide tolerance of four (SP) varieties (Beauregard, Orleans, Dianne, and Covington) were evaluated in a screening study. These varieties were selected to represent sweetpotato production areas across the United States in Arkansas, California, Louisiana, Mississippi, and North Carolina. Herbicides evaluated include fluridone, glyphosate, glufosinate, carfentrazone, saflufenacil, acifluorfen, and others. The study involved the foliar application of herbicide treatments at 1X and 0.5X rates, laid out in a completely randomized design, using a spray chamber onto individual (SP) plants cultivated in 4x4 inch containers within the controlled environment of the Dorman Hall Greenhouse at Mississippi State University. Visual assessments of herbicide-induced injury were conducted at regular intervals of 7, 14, 21, and 28 days after treatment (DAT), accompanied by measurements of vine length in centimeters. Furthermore, dry root and shoot biomass were quantified at 28 DAT to provide a comprehensive assessment of herbicide impacts on (SP) growth and development. Data was analyzed using ANOVA and means separated by Fisher’s protected LSD (α=0.05). Results indicate varying levels of tolerance among the (SP) varieties to specific herbicides and application rates. Noteworthy trends in visual injury, vine length, and biomass measurements highlight the nuanced responses of the cultivars to different herbicide chemistries.
Speakers Co-authors
MS

Mark Shankle

Mississippi State University
Wednesday September 25, 2024 4:45pm - 5:00pm HST
South Pacific 3

4:50pm HST

CHEX/FP - Effect of Planting Date on Strawberry Yield
Wednesday September 25, 2024 4:50pm - 5:00pm HST
Optimizing planting date for strawberry in California production is a sustainable measure to maximize yield and maintain plant health. The goal of this project is to assess the optimal planting date for two predominant cultivars: 'Monterey' and 'Fronteras'. The trial was conducted in field 25 at Cal Poly, San Luis Obispo. The experimental area consisted of 3 beds, each 53.5 ft. long. Standard 64-inch beds with 4 rows of plants per bed and 3 lines of drip tape were used. Beds were planted at two-week intervals: 26 Oct, 9 Nov, 23 Nov 2022. Each bed was planted with four plots of ‘Monterey’ and four plots of ‘Fronteras’ (20 plants/plot). Plug plants were produced at North Carolina State University’s nursery and shipped overnight to Cal Poly and planted in the field the next day. First harvest was 13 Apr 2023 when the first fully red fruit were observed. Fruit were harvested, counted, and weighed twice weekly. The trial was completed 8 Aug 2023 and replicated in the 2024 growing season.
Speakers
SS

Samantha Simard

Cal Poly- San Luis Obispo
Wednesday September 25, 2024 4:50pm - 5:00pm HST
Lehua Suite

4:55pm HST

CE 2 - Effects of Mycorrhiza and Biochar Interactions on Geraniums
Wednesday September 25, 2024 4:55pm - 5:05pm HST
Biochar has long been proposed to be a substitute for peat in soilless mixes for greenhouse growing. Low levels of biochar have been shown to increase disease resistance, increase nutrient supply and uptake, and immobilize phytotoxic substances. Due to its high porosity and pH, biochar has the potential to provide an ideal habitat for mycorrhizal fungi to partner with plant roots. This study examined how various mycorrhiza sources interacted with different biochar rates to effect geraniums (Pelargonium x hortorum L. ‘Maverick Red’). Four different mycorrhizal sources were used in addition to a control containing no mycorrhiza: two commercial sources, MycoBloom and BioAg Vam-Endo, spores extracted from agricultural soils, and spores extracted from prairie soil, with four biochar rates implemented: 0, 15, 30, and 45%. Media with biochar incorporated remained saturated for longer periods after irrigation than pots filled with straight BM-7 peat-media. Prairie soil combined with 15% biochar-BM7 media formed buds and began to flower before all other treatments. Results suggest that biochar and mycorrhiza may pair well to improve potted plant growing.
Speakers
TH

Teal Hendrickson

Oklahoma State University
Co-authors
BD

Bruce Dunn

Oklahoma State University
GW

Gail Wilson

Oklahoma State University
NA
Wednesday September 25, 2024 4:55pm - 5:05pm HST
Coral 2

5:00pm HST

CH/FP - Outcomes of the Western Sustainable Agriculture Research and Education Summit in New Mexico
Wednesday September 25, 2024 5:00pm - 5:10pm HST
The aims of the federal initiative Sustainable Agriculture Research and Education (SARE) are multifaceted: 1) Foster responsible management of the nation's natural resources through the dissemination of location-specific, regional, and sustainable agricultural and ranching techniques, 2) Improve the livelihoods of farmers and ranchers, 3) Safeguard the well-being of individuals involved in food and agricultural systems by reducing reliance on harmful substances, 4) Encourage agricultural variety and resilience, and 5) Assess the local economic, social, and environmental impacts of adopting sustainable agricultural practices. To achieve these goals, SARE offers numerous grant opportunities for research projects, catering to farmers, ranchers, non-governmental organizations, and universities. SARE operates across four regions within the United States. New Mexico is in the Western region (WSARE) which is composed of individual state professional development programs in the area’s 13 states and 4 Pacific Island protectorates. In 2023, the NM WSARE Professional Development Program coordinated a Sustainable Agriculture Summit to better prioritize research and training needs in the state. The event was held in Santa Fe, NM, USA on 30 March, with a total of 58 participants in the full-day event. Among them were 32 agriculture professionals and 26 farmers and ranchers. The event consisted of two main sessions, a panel discussion, followed by an open forum. The panel was comprised of past Western SARE grant recipients and individuals who had submitted proposals, they provided overviews of their projects. Following the panel, summit participants engaged in a brainstorming session to identify critical sustainable agriculture project needs for producers in NM, focusing on research and training needs not previously emphasized in past funding. The priority areas identified by the participants were, in order of importance, 1) Irrigation/water use efficiency, 2) Farm economics, direct markets, and direct sales, 3) Non-traditional production, 4) Pest management, 5) Labor aids and technology adaptation, and 6) Generational transfer of farms and ranches. Each of these priorities was clearly outlined, and potential projects were identified accordingly. The outcomes of this summit were communicated to WSARE, the NM Department of Agriculture, and other funding agencies, offering valuable feedback to guide future proposals and funding initiatives in NM.
Speakers
ME

Mariela Estrada

New Mexico State University
Co-authors
IJ

Israel Joukhadar

New Mexico State University
Wednesday September 25, 2024 5:00pm - 5:10pm HST
Lehua Suite

5:00pm HST

WCPM 2/IPR - Assessment of Various Carbon Sources for Anaerobic Soil Disinfestation to Manage Weeds in Organic Sweetpotato
Wednesday September 25, 2024 5:00pm - 5:15pm HST
Yellow nutsedge (Cyperus esculentus L.) management in organic plasticulture systems is challenging as it reproduces both by seeds and tubers. Yellow nutsedge has a strong midrib and sharp leaf tip which allows it to puncture plastic mulch and creates favorable conditions for other weeds to grow, compete for resources with crop plants, and decrease crop yield. Lack of available herbicide options in specialty crops make weed management more challenging. Anaerobic soil disinfestation (ASD) is a technique that has shown potential to manage weeds in organic production systems. ASD is facilitated by incorporating carbon sources into the soil, tarping the soil with plastic mulch, and irrigating to the soil saturation. A field study was conducted at Clemson University’s Coastal Research and Education Center, Charleston, South Carolina, to evaluate the impact of various carbon sources in ASD on weed management in organic sweetpotato. This treatment structure for this study consisted of a factorial with four carbon source treatments (cotton seed meal, chicken manure molasses, brassica waste, and non-amended control) and four sweetpotato cultivars (Bayyou Belle, Muraski, Monaco, and USDA 18-040). These sweetpotato cultivars have two different growth habits, either bunch type (USDA 18-040 and Monaco) or spreading type (Bayyou Belle and Muraski). The primary purpose of using different plant architecture is to evaluate the impact of the sweetpotato vine growth habit on weed emergence. Experimental plots receiving chicken manure molasses and cotton seed meal as carbon source resulted in the greatest cumulative anaerobic conditions (
Speakers
SS

Simardeep Singh

Clemson University
Co-authors
Wednesday September 25, 2024 5:00pm - 5:15pm HST
South Pacific 3

5:00pm HST

Executive Committee Meeting of the American Pomological Society
Wednesday September 25, 2024 5:00pm - 7:00pm HST
Moderator
avatar for Ksenija Gasic

Ksenija Gasic

Clemson University
Wednesday September 25, 2024 5:00pm - 7:00pm HST
Sea Pearl 3

5:05pm HST

CE 2 - Exploring Different Nutrient Solution Quantities to Optimize Quantity and Quality in Hydroponic Production
Wednesday September 25, 2024 5:05pm - 5:15pm HST
Nutrient solutions play a crucial role in determining crop yield and quality, with optimized quantities offering sustainability benefits. However, there is a lack of comprehensive research regarding the optimal nutrient application quantity for various leafy green vegetables in recirculating hydroponic cultivation. To address this research gap, we proposed a project on different nutrient application quantities using the nutrient film technique (NFT) hydroponic system in a greenhouse with three replications during the fall (air temp: 24.22°C, RH: 31.2%), winter (air temp: 15.5 °C, RH: 73.3%) and early spring (air temp: 13.7 °C, RH: 72.4% ). The project focused on exploring different nutrient solution quantities of Low (76 liters), Medium (114 liters), and High (151 liters) nutrient regimens for six different leafy green vegetable species and cultivars common in Kansas including red butter lettuce (Lectuca sativa), green butter lettuce (Lectuca sativa), arugula (Eruca sativa), kale (Brassica oleracea), red malabar spinach (Basella alba), and basil (Ocimum basilicum). Our results showed that green butter lettuce and basil remained unaffected by the treatments throughout the study. Additionally, plant height, leaf count, and SPAD value for all species remained consistent across treatments and seasons. However, during the fall, the shoot fresh weight of red butter lettuce and kale increased by 7.11% and 21.1%, respectively, in the high-nutrient regimen. Moreover, the dry shoot weight of kale increased by 18.7% in the high-nutrient regimen, while the dry shoot weight of the red malabar spinach increased by 10.3% in the low-nutrient regimen. In contrast, during winter, the shoot fresh weight of red butter lettuce increased by 18.9% and 25.0%, respectively, in medium nutrient regimens compared to low and high nutrient regimens. Similarly, the shoot fresh weight of red malabar spinach increased by 15.3% and 25.0%, respectively, in low-nutrient regimens compared to medium and high-nutrient regimens. During early spring, the shoot fresh weight of red butter lettuce increased by 17.9-18.0% and that of arugula increased by 17.8% in the high-nutrient regimen, compared to low and medium nutrient regimens. In summary, the high-nutrient regimen benefited red butter lettuce and kale in fall and arugula in early spring. Conversely, during winter, the medium nutrient regimen benefited red butter lettuce, while the low nutrient regimen benefited red malabar spinach. The results from this experiment identified the optimal nutrient application quantity which helps to reduce nutrient waste for vital leafy vegetables in Kansas for different seasons and offers valuable production guidelines for local growers.
Speakers
avatar for Puja Subedi

Puja Subedi

Kansas State University
I am a graduate research assistant working at the Kansas University Olathe, 22201 W Innovation Dr, Olathe, KS 66061. My major is urban food system under the department of horticulture and natural resources. Currently, I am working in the hydroponics research project.
Co-authors
TY

Teng Yang

Kansas State University
Wednesday September 25, 2024 5:05pm - 5:15pm HST
Coral 2

5:15pm HST

CE 2 - How Does Supplementing Dissolved Oxygen Impact Hydroponic Strawberry Production?
Wednesday September 25, 2024 5:15pm - 5:25pm HST
Oxygen is crucial for the growth and nutrient uptake of plant roots, especially in crops like strawberries that demand high levels of oxygen in their root zones. However, in hydroponic systems, the nutrient solution is often inadequately oxygenated. In this study, we examined the effects of supplementing dissolved oxygen (DO) into the nutrient solution on the growth of strawberry plants. Inside an indoor vertical farm, bare-root plants of strawberry ‘Albion’ and ‘Eversweet’ were grown using deep water culture hydroponics under a controlled environment of 23 °C air temperature and an 18-h photoperiod, with an extended photosynthetic photon flux density of 350 µmol∙m –2 ∙s –1 . The DO concentration of the nutrient solution was maintained at control levels (no adjustment) or supplemented using an air pump or an oxygen concentrator. The average DO concentrations in the control condition was 70%, while supplementing the nutrient solution with an air pump or an oxygen concentrator increased the average DO concentration to 85% and 100%, respectively. Supplementing with DO had minimal to no effect on the days to root of strawberry bare root plants in both cultivars. Four weeks after the DO treatments, root length, crown diameter, leaf area, and fresh mass of shoot and root were also similar in both cultivars regardless of DO concentration. The effects of supplementing DO on flowering and fruit production will also be presented.
Speakers
JR

Jonathan Ries

Arizona State University
Co-authors
YP

Yujin Park

Arizona State University
Wednesday September 25, 2024 5:15pm - 5:25pm HST
Coral 2

5:15pm HST

WCPM 2/IPR - Evaluation of saturated steam with boiling water to control Guinea grass (Megathyrsus maximus) in riparian landscapes in Hawaii.
Wednesday September 25, 2024 5:15pm - 5:30pm HST
Boiling water and steam have been effectively used as a non-chemical means to control weeds in croplands and urban areas. In Hawaii, it is a relatively new technology with limited trials done on local conditions and weeds. Guinea grass (Megathyrsus maximus) is a noxious weed that invades landscapes and agricultural fields in Hawaii and throughout the world. While it is typically controlled using herbicide sprays, this may be restricted if the guinea grass being controlled is located in riparian areas. In this study, we evaluated the efficacy of saturated steam with boiling water in controlling mature clumps of guinea grass growing along a streambank. Guinea grass bunches were divided into small (less than 15.2 cm) and large clumps (15.2 to 30.5 cm) and then cut 5 to 15 cm from the ground. Cut clumps were either not treated (control) or treated with saturated steam and boiling water for 1 minute using a 15cm long spike injector inserted into different points of the crown. Percent green color and number of resprouts were collected 7, 14, and 21 days after application. Dry biomass was collected 22 days after application. Results indicate that saturated steam with boiling water was effective in controlling guinea grass clumps. Small clumps were completely controlled (no resprouts and new biomass) while large clumps had significantly reduced the number of resprouts and reduced production of new biomass. Saturated steam with boiling water offers a non-chemical means to control guinea grass in riparian areas in Hawaii.
Speakers
avatar for Hannah Lutgen

Hannah Lutgen

Extension Faculty, University of Hawaii at Manoa, College of Tropical Agriculture & Human Resources (CTAHR)
As an Extension Agent faculty member at the University of Hawaiʻi at Mānoa College of Tropical Agriculture and Human Resources (CTAHR), I analyze issues, create programs, and conduct activities that meet client needs to support landscape professionals, ornamental and cut flower... Read More →
Co-authors
OB

Orville Baldos

University of Hawaii at Manoa
RG

Rosemary Gutierrez Coarite

University of Hawaii at Manoa
Wednesday September 25, 2024 5:15pm - 5:30pm HST
South Pacific 3

5:25pm HST

CE 2 - Exploring Different EC Levels to Optimize Quantity and Quality in Hydroponic Production
Wednesday September 25, 2024 5:25pm - 5:35pm HST
Efficient nutrient management is the key to successful hydroponic production. However, there is a lack of comprehensive research regarding the optimal electrical conductivity (EC) levels for various leafy green vegetables in recirculating hydroponic cultivation. To address this research gap, we experimented with different EC levels using the nutrient film technique (NFT) hydroponic system in a greenhouse with three replications during the fall (air temp: 24.22°C, RH: 31.2%) winter (air temp: 15.5 °C, RH: 73.3%) and early spring (air temp: 13.7 °C, RH: 72.4%). The experiment was conducted using three different EC levels (1.2, 1.8, and 2.4 mS/cm) for six different leafy green vegetables kale (Brassica oleracea) ‘Winter bor F1’ and ‘Toscano’, swiss chard (Beta vulgaris), basil (Ocimum basilicum) ‘Prospera® Compact DMR (PL4)’ and ‘large leaf’ and red malabar spinach (Basella alba). Our results showed that, during the fall, the shoot fresh weight of the ‘Winter bor F1’ increased by 13.1 % in EC 2.4 compared to EC 1.2 and that of Swiss chard increased by 8.3-20.6% in EC 2.4 compared to EC 1.2 and 1.8 while that of ‘Prospera® Compact DMR (PL4)’ basil increased by 13.1-13.9 % in EC 1.8 compared to EC 1.2 and 2.4. In contrast during the winter, the shoot fresh weight of ‘Toscano’ kale, ‘Winter bor F1’ kale, and ‘large leaf’ basil increased by 11.2-17.8%, 18.9-20.8%, and 13.2-14.7%, respectively in EC 2.4 compared to EC 1.2 and 1.8, while that of ‘Prospera® Compact DMR (PL4)’ basil increased by 19.2 % in EC 1.2 compared to the EC 2.4. However, during the early spring, only the shoot fresh weight of ‘Winter bor F1’ kale in EC 1.8 was increased by 10.0 % compared to EC 1.2, while the plant height and fresh shoot weight of large leaf basil was increased slightly by 3.1-5.6% in EC 2.4 compared to EC 1.2. In summary, this experience suggested that ’Winter bor F1’ kale performed best in EC 2.4 during the fall and winter seasons but grew best in EC 1.8 during the early spring. In addition, the 2.4 mS/cm proved the optimal EC level for Swiss chard during the fall, ‘Toscano’ kale during the winter, and ‘large leaf’ basil during the spring. The results from this experiment identify optimal EC levels of vital leafy vegetables in Kansas for different seasons, aiding Kansas growers in reducing nutrient waste and enhancing leafy vegetable production.
Speakers
avatar for Puja Subedi

Puja Subedi

Kansas State University
I am a graduate research assistant working at the Kansas University Olathe, 22201 W Innovation Dr, Olathe, KS 66061. My major is urban food system under the department of horticulture and natural resources. Currently, I am working in the hydroponics research project.
Co-authors
TY

Teng Yang

Kansas State University
Wednesday September 25, 2024 5:25pm - 5:35pm HST
Coral 2

6:00pm HST

ASHS Fellows Meet and Greet
Wednesday September 25, 2024 6:00pm - 7:00pm HST
All ASHS Fellows are invited to meet at the Bali Oceanfront Restaurant.
Speakers
avatar for Sandra Wilson

Sandra Wilson

Professor, University of Florida
Dr. Sandra Wilson is a Professor of Environmental Horticulture at the University of Florida’s main campus in Gainesville. She received B.S. and M.S. degrees from the University of Delaware and a Ph.D. in Plant Physiology from Clemson University. Dr. Wilson completed postdoctoral... Read More →
avatar for Curt Rom

Curt Rom

University Professor. And Associate Director, University of Arkansas and ASHS
Wednesday September 25, 2024 6:00pm - 7:00pm HST
Bali Oceanfront Restaurant Rainbow Tower

6:00pm HST

Graduate Student Poster Competition
Wednesday September 25, 2024 6:00pm - 7:30pm HST
Competition participants must bring your poster pdf on a thumb drive or the physical poster to your assigned room. You will be lined up to present to the judges in order of arrival. You will enter the room one at a time.

Students will be given 5 minutes to make a presentation to the judges, followed by a 2 minute period of questions and answers.

This competition is open to graduate students that have a poster presentation scheduled during the ASHS conference AND have also signed up to participate in this competition (Note: This is separate of the assigned time to present your abstract during the conference program).

Please note that if you do not also present your poster during the regularly scheduled Poster session, you will be disqualified from the Poster Competition.


Speakers
HV

Hannah Voye

South Dakota State University
avatar for Carly Strauser

Carly Strauser

Iowa State University
Graduate Research Assistant, Department of Horticulture at Iowa State University
SS

Simardeep Singh

Clemson University
EE

Erika Escalona

graduate student, UC Davis
MU

Merci Uwimbabazi

Graduate Research Assistant, Kentucky State University
BSc in Conservation Agriculture / Pursuing a Master’s in Environmental Studies. Highly interested in Controlled Environment Agriculture and Horticulture.
avatar for Oluwatunmise Israel Dada

Oluwatunmise Israel Dada

Graduate Research Assistant, Washington State University
A Ph.D. Candidate passionate about the natural environment and sustainable solutions.
RN

Richard Noel Torres

University of Missouri - Columbia
avatar for Jeanine Arana

Jeanine Arana

Purdue University

Wednesday September 25, 2024 6:00pm - 7:30pm HST
South Pacific 1

6:00pm HST

Graduate Student Poster Competition
Wednesday September 25, 2024 6:00pm - 7:30pm HST
Competition participants must bring your poster pdf on a thumb drive or the physical poster to your assigned room. You will be lined up to present to the judges in order of arrival. You will enter the room one at a time.

Students will be given 5 minutes to make a presentation to the judges, followed by a 2 minute period of questions and answers.

This competition is open to graduate students that have a poster presentation scheduled during the ASHS conference AND have also signed up to participate in this competition (Note: This is separate of the assigned time to present your abstract during the conference program).

Please note that if you do not also present your poster during the regularly scheduled Poster session, you will be disqualified from the Poster Competition.

Speakers
avatar for Kondwani Kamsikiri

Kondwani Kamsikiri

University of Florida
MP

Monika Peddapuli

UNIVERSITY OF FLORIDA
NU

Noor Ul Basar

University of Florida
EW

Emily Worbington

University of Florida-IFAS CREC
GB

Giancarlo Buzzi

University of Florida
MT

MADELEINE TENNY

Florida Gulf Coast University
avatar for Lucas Sales

Lucas Sales

Research Assistant, University of Georgia
Agronomy Engineer graduated from the Federal University of Paraíba. With experience in the management and cultivation of Ornamental Plants, through a year of experience working in Greenhouses in the state of New Hampshire, USA. Experienced in the management and cultivation of vegetables... Read More →
JB

Joseph Bazzle

Clemson University

Wednesday September 25, 2024 6:00pm - 7:30pm HST
Coral 1

6:00pm HST

Graduate Student Poster Competition
Wednesday September 25, 2024 6:00pm - 7:30pm HST
Competition participants must bring your poster pdf on a thumb drive or the physical poster to your assigned room. You will be lined up to present to the judges in order of arrival. You will enter the room one at a time.

Students will be given 5 minutes to make a presentation to the judges, followed by a 2 minute period of questions and answers.

This competition is open to graduate students that have a poster presentation scheduled during the ASHS conference AND have also signed up to participate in this competition (Note: This is separate of the assigned time to present your abstract during the conference program).

Please note that if you do not also present your poster during the regularly scheduled Poster session, you will be disqualified from the Poster Competition.

Speakers
AN

ASMITA NAGILA

Graduate Research Assistant, Texas A&M university
avatar for Lilin Chen

Lilin Chen

Graduate Research Assistant, University of Georgia
I'm interested in biochar effect on containerized plants, especially the effect under abiotic stresses such as drought and salinity.
avatar for Saroj Burlakoti

Saroj Burlakoti

Utah State University
ST

Sentaro Tomiyama

Graduated student, Meiji University
XC

Xiangyu Cui

Meiji University
avatar for Emilio Suarez Romero

Emilio Suarez Romero

Research Assistant, University of Georgia
TH

Teal Hendrickson

Oklahoma State University
avatar for Puja Subedi

Puja Subedi

Kansas State University
I am a graduate research assistant working at the Kansas University Olathe, 22201 W Innovation Dr, Olathe, KS 66061. My major is urban food system under the department of horticulture and natural resources. Currently, I am working in the hydroponics research project.

Wednesday September 25, 2024 6:00pm - 7:30pm HST
South Pacific 3

6:00pm HST

Graduate Student Poster Competition
Wednesday September 25, 2024 6:00pm - 7:30pm HST
Competition participants must bring your poster pdf on a thumb drive or the physical poster to your assigned room. You will be lined up to present to the judges in order of arrival. You will enter the room one at a time.

Students will be given 5 minutes to make a presentation to the judges, followed by a 2 minute period of questions and answers.

This competition is open to graduate students that have a poster presentation scheduled during the ASHS conference AND have also signed up to participate in this competition (Note: This is separate of the assigned time to present your abstract during the conference program).

Please note that if you do not also present your poster during the regularly scheduled Poster session, you will be disqualified from the Poster Competition.

Speakers
PP

Pradip Poudel

The Penn State University
Pradip
ZW

Zirui Wang

Utah State University
GS

Gursewak Singh

Graduate Research Assistant, Clemson University
JA

Jannatul Afroze

New Mexico State University
avatar for Lindsey Trinkle
avatar for Noah Willsea

Noah Willsea

Clemson University
avatar for I. Alejandra Sierra

I. Alejandra Sierra

University of Florida

Wednesday September 25, 2024 6:00pm - 7:30pm HST
South Pacific 2

6:00pm HST

Graduate Student Poster Competition
Wednesday September 25, 2024 6:00pm - 7:30pm HST
Competition participants must bring your poster pdf on a thumb drive or the physical poster to your assigned room. You will be lined up to present to the judges in order of arrival. You will enter the room one at a time.

Students will be given 5 minutes to make a presentation to the judges, followed by a 2 minute period of questions and answers.

This competition is open to graduate students that have a poster presentation scheduled during the ASHS conference AND have also signed up to participate in this competition (Note: This is separate of the assigned time to present your abstract during the conference program).

Please note that if you do not also present your poster during the regularly scheduled Poster session, you will be disqualified from the Poster Competition.

Speakers
OG

Orestis Giannopoulos

University of Georgia
AG

Amit Godara

The University of Georgia
GC

Gasselle Cordova

University of Florida
avatar for Juan David Jimenez Pardo

Juan David Jimenez Pardo

PhD student, University of Florida
KL

Katelyn Lust-Moore

University of Arkansas
avatar for Venkateswara Rao Kadium

Venkateswara Rao Kadium

Montana State University
MR

MEGAN REID

University of Maryland Eastern Shore

Wednesday September 25, 2024 6:00pm - 7:30pm HST
Coral 2

6:00pm HST

Graduate Student Poster Competition
Wednesday September 25, 2024 6:00pm - 7:30pm HST
Competition participants must bring your poster pdf on a thumb drive or the physical poster to your assigned room. You will be lined up to present to the judges in order of arrival. You will enter the room one at a time.

Students will be given 5 minutes to make a presentation to the judges, followed by a 2 minute period of questions and answers.

This competition is open to graduate students that have a poster presentation scheduled during the ASHS conference AND have also signed up to participate in this competition (Note: This is separate of the assigned time to present your abstract during the conference program).

Please note that if you do not also present your poster during the regularly scheduled Poster session, you will be disqualified from the Poster Competition.

Speakers
TC

Thiago Campbell

Washington State University
BW

Bryce Waugh

Grad Student, Oklahoma State University
avatar for Min Jeong Kang

Min Jeong Kang

Graduate Research Assistant, University of Georgia
Hello! I am a 5th year Ph.D. candidate in the Food Science and Technology Department at the University of Georgia.My research interests are Metabolomics, using Machine Learning Algorithms to discover the biomarkers. My recent work involves evaluating pecan quality, looking at biomarkers... Read More →
LS

Laura Schulz

Graduate Research Assistant, Washington State University
I am a PhD student at Washington State University working with Carol Miles on introducing sweetpotato as a new crop in western Washington.
SS

Sadikshya Sharma

University of California Davis
I am a PhD candidate working on grape breeding and genomics at the University of California Davis. I am working on developing high-throughput phenotypic and genomic tools for salt and drought tolerance in grapes.
avatar for Pragya Dhakal Poudel

Pragya Dhakal Poudel

The University of Queensland
QF

Qiuyi Fu

Michigan State University

Wednesday September 25, 2024 6:00pm - 7:30pm HST
Nautilus

6:00pm HST

Graduate Student Poster Competition
Wednesday September 25, 2024 6:00pm - 7:30pm HST
Competition participants must bring your poster pdf on a thumb drive or the physical poster to your assigned room. You will be lined up to present to the judges in order of arrival. You will enter the room one at a time.

Students will be given 5 minutes to make a presentation to the judges, followed by a 2 minute period of questions and answers.

This competition is open to graduate students that have a poster presentation scheduled during the ASHS conference AND have also signed up to participate in this competition (Note: This is separate of the assigned time to present your abstract during the conference program).

Please note that if you do not also present your poster during the regularly scheduled Poster session, you will be disqualified from the Poster Competition.

Speakers
PN

PRAKRITI NEPAL

Utah State Univeristy
avatar for CYRUS JANUARIE

CYRUS JANUARIE

Graduate Research Assistant, UNIVERSITY OF FLORIDA
Soil nutrient managementStatistical data analysisField research 
JH

Jessica Hutchinson

University of Maine
avatar for Yulong Chen

Yulong Chen

The University of Georgia
avatar for Amanda Jessica Da Silva

Amanda Jessica Da Silva

Research Assistant, Michigan State University
NK

Navdeep Kaur

Texas A and M university
MK

Mark Kindred

Grad Student, Utah State University
I am a graduate student at Utah State University working on my master's degree in Dr Jennifer Reeve's lab. I got my bachelor's in plant science and Asian studies from Utah State University. While I was an undergrad, I worked in Jennifer Reeve's lab. When I graduated, I was offered... Read More →

Wednesday September 25, 2024 6:00pm - 7:30pm HST
South Pacific 4

6:00pm HST

Association of Horticulturists of Indian Subcontinent (AHIS) Meeting
Wednesday September 25, 2024 6:00pm - 7:30pm HST
Moderator
SB

Samikshya Bhattarai

Graduate Research Assistant, Texas A&M University
Wednesday September 25, 2024 6:00pm - 7:30pm HST
Sea Pearl 4

6:30pm HST

Asian Horticulture Professional Interest Group Gathering
Wednesday September 25, 2024 6:30pm - 9:30pm HST
Speakers
XW

Xingbo Wu

Chair 2023-2024, University of Florida
NA
JM

Jianbing Ma

Chair-elect
YM

Yu Ma

Secretary
Wednesday September 25, 2024 6:30pm - 9:30pm HST
Hibiscus
 


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