Pairwise is a technology-focused food and agriculture company that makes tiny alterations to plant genetics to solve big problems. Powered by our best-in-class technology, Pairwise is harnessing new genomics technologies to create innovative new products. With our FulcrumTM platform, we accelerate innovation in plants and plant-based production systems, delivering value to people and our planet. Partnering with Plant Sciences, Inc. to access their elite germplasm, we created a platform to transform and edit tetraploid blackberry (Rubus sub. Rubus). We used the power of genetics and genomic tools to identify the candidate gene for thornless in blackberries and used CRISPR based gene editing to validate the trait. The identification of this gene will enable faster deployment of a thornless trait, an essential production trait, into blackberries. The gene may be broadly applicable to other Rubus species and horticultural crops such as roses.