This study addresses the critical need for precise irrigation management in the greenhouse production of high-value ornamental crops, focusing on the propagation of single-stem rose (Rosa rubiginosa) cuttings under light-emitting diode (LED) supplemental lighting. The current lack of effective monitoring and control systems for substrate moisture poses challenges in optimizing plant growth while minimizing water and nutrient losses. In this context, we propose the integration of moisture sensors for real-time monitoring and control of substrate moisture levels, coupled with LED supplemental lighting, to enhance the production of rose cuttings. Our approach involved assessing the feasibility and effectiveness of moisture sensor-controlled irrigation in greenhouses, considering the specific requirements of rose propagation and the influence of LED lighting on plant growth. We tested three Ө thresholds (0.25, 0.35, and 0.45 m3.m-3) and five light levels as supplemental lighting (100, 175, 250, 325, and 400 µmol.m-2.s-1) arranged on randomized complete block design with four replications. Rose Double Knock Out® ‘Radtko’ PP 16,202 CPBR 3,104 plants were grown in 15.6 L pots (Pioneer Pots; Blackmore Co., Belleville, MI) filled with 20% Canadian peat/58% aged pine/10% perlite/12% EZ Hydrafiber lime potting mix (Oldcastle HFC25; Oldcastle Lawn
