The goal of the project is to evaluate the potential of reducing chemical fumigation in California's watermelon production using grafting and Trichoderma-based biofungicide. Two field experiments in 2022 and 2023 were implemented within commercial watermelon fields in Stockton and Modesto, CA. Each trial included three rootstocks (Cobalt, Flexifort, and RS841) grafted onto a 45-ct scion (Summer Breeze). Two Trichoderma-containing products were applied to the grafted and nongrafted seedlings through tray soaking at one day before transplanting or field chemigation at 30 and 62 days after transplanting (DAT). The application rates for soaking and chemigation were 45 g.75 L-1 of water and 2.2 kg.ha-1, respectively. All seedlings were mechanically transplanted during mid-May of both years into a split-split plot design with the Trichoderma product as the main factor and application method as the sub-plot. All treatments were replicated four times. Each treatment row was 18.6 m long and contained ten grafted or nongrafted triploids and three grafted or nongrafted pollenizers. Vine health was visually assessed three times for symptoms associated with soil-borne fungal pathogens. Canopy coverage was measured starting at 22 DAT in 2022 and 40 DAT in 2023 and thereafter at an approximate interval of two weeks for a total of six measurements. Harvest was conducted three times in 2022 and twice in 2023 to analyze yield and quality differences among treatments. Runner and root samples were taken amid the harvest and shipped to the UC Davis Fungal Pathology Lab for further identification. Results indicated that the Trichoderma-containing bio-fungicides were not as effective as grafting on preventing vine decline and maintaining canopy coverage in both trials. The synergistic effects of grafting and Trichoderma inoculation provided some but limited benefits to plant health compared to the single factor of grafting. The overall influence of biofungicides on yield was also less notable than that of grafting regardless of application method. For fruit quality, grafting dominated the impacts over biofungicides on fruit firmness and rind thickness in both trials. Charcoal rot (Macrophomina phaseolina) with other putative co-infestations of Fusarium pathogens were diagnosed from nongrafted inoculation-free plots in 2022 while no significant soil-borne diseases were identified from 2023. Overall, grafting with multi-pathogen resistant rootstocks prove to be a reliable method for enhancing watermelon yield while maintaining crop health. More work is still needed to further explore the optimal application protocols tailored to grower’s farming system to maximize the effects of biofungicide.
