Cultivated strawberry (Fragaria ×ananassa Duch, 2n = 8× = 56) belongs to the family Rosaceae and is an allo-octoploid species. We report here a high-quality haplotype-phased genome assembly of a representative Korean short-day cultivar, ‘Sulhyang’ using a trio-binning approach. Using PacBio Revio long reads of ‘Sulhyang’ and whole genome paired-end reads (Illumina) of two parents ‘Akihime’, and ‘Yukbo’, we completed telomere-to-telomere phased genome assemblies of both haplotypes. The ‘Sulhyang’ genome was 797 Mb , with a long terminal repeat (LTR) assembly index (LAI) of 17.28. The genome was phased into two haplotypes, phase-1 (784.9 Mb with contig N50 of 12.34) and phase-2 (781 Mb with contig N50 of 16.04 Mb). The BUSCO analysis detected over 99% of conserved genes in the combined phased-1 and phased-2 assembly. Both haploid assemblies were annotated using RNA-Seq data representing various F. ×ananassa tissues from the NCBI sequence read archive, resulting in a total of 104,099 genes in the primary assembly. This haplotype-phased reference genome for ‘Sulhyang’ will broaden our knowledge of strawberry genome evolution and gene functions.
