Osmanthus is a genus of ornamental plants with valuable qualities such as pest resistance, evergreen foliage, and aromatic flowers. However, different species respond differently to growth hormones for propagation. This study aimed to evaluate the types and rates of plant growth hormones on different species of Osmanthus propagation. Six distinct Osmanthus species (Osmanthus heterophyllus ‘Kaori Hime’, Osmanthus armatus ‘Jim Porter’, Osmanthus x fortunei ‘Patty's Secret’, Osmanthus heterophyllus ‘Rotundifolius’, Osmanthus delavayi, and Osmanthus x fortunei ‘Fruitlandii’) were treated with Indole-3-butyric acid (IBA) powder, 500 mg/L and 2000 mg/L of Potassium Indole-3-butyric acid (K-IBA), 10% Kelpak (seaweed extract), and water dip (the control). The cuttings’ survival rates, rooting rates, callus rate and area, and root length were recorded. The results showed the O. heterophyllus ‘Kaori Hime’ exhibited the best survival and rooting rates, while the O. heterophyllus ‘Rotundifolius’ had the lowest survival rate, the O. armatus ‘Jim Porter’ had the highest callus quantity. Plants treated with IBA powder had significantly larger callus area than those seaweed treatments. In conclusion, O. x fortunei 'Fruitlandii' and O. heterophyllus 'Kaori Hime' exhibit superior performance in all six measurements compared to other plant species and are highly recommended. Conversely, O. delavayi and O. heterophyllus 'Rotundifolius' have displayed considerably weaker performance and are not recommended. Further research is necessary to determine the effectiveness of other propagation methods and treatments for Osmanthus.
