Our research is to define and develop pre-breeding resources as foundational knowledge to underpin breeding of a specialty leafy green crop watercress (Nasturtium officinale; Brassicaceae). This is being achieved by screening a unique, worldwide collection of watercress population to discover and to enhance nutritional traits for health, morphology, and sensory of the indoor controlled environment agriculture (CEA) market. Watercress is a perennial semi-aquatic leafy green vegetable in the Brassicaceae family and is an understudied specialty crop that has important human health benefits. The most abundant secondary metabolite glucosinolate (GLS) in watercress is gluconasturiin, an aromatic GLS, which hydrolyses and releases phenethyl-isothiocyanates (PEITC). PETIC, specifically from watercress, has been proven to have chemo-preventative potentials. Wild germplasm collection harbours natural variations and useful trait discovery opportunities for introgression of novel traits into the existing gene pool. There is limited interdisciplinary research on crop nutrition and breeding for the CEA settings. We found that watercress is well-suited to indoor hydroponic growing. We established the first indoor vertical farm (VF), a controlled growth chamber in a shipping container, at University of California, Davis. Light quality and quantity both serve important roles in watercress growth and development, and a fully controllable vertical farm allows testing a suite of traits of interests with altered LED light regimes. Results showed that VF grown wild watercress possessed significant genotypic differences across treatments, indicating an abundant natural diversity. Altering red to blue LED light ratio and duration may further enhance the anti-cancer GLS compounds as well as nutritional quality profile of this leafy crop.