Lettuce (Lactuca sativa L.) is a leafy vegetable in the Asteraceae family and is classified into various types, such as Romaine, Butterhead, Crisphead (Iceberg and Batavian), Latin, Leaf, and Stem. Lettuce propagates by the seeds. The seedling can grow in soil or a hydroponic system in a controlled environment Agriculture (CEA) system. This study was conducted to study the ability of different types of lettuces to grow in a hydroponic system and to assess the effect of light, humidity, and temperature on the yield. The experiment was conducted twice (2023 and 2024) in the Department of Agricultural Science lab at Morehead State University. Four lettuce cultivars, including 'Gustav's Salad' and 'May Queen' as the Butterhead type, 'Ice Queen' as a Crisphead type, and 'Lollo' as the Leaf type, were planted directly on rock wools (Rockwool Starter Plugs) in 2-inch Garden Net Pot in hydroponic containers. The commercial aquarium pumps constantly aerated the nutrient solution. Plants were fertilized with liquid plant food, and the Standard Hydroponic solution controlled the pH. The artificial light was provided by a mix of different lights (µmol/m2/s), including LEC, LED, and Fluorescent, for 16 hours daily under different temperatures (°C) and humidity (%). Biological control products were used for pest and disease control. Harvesting was done after 60 days, and fresh and dried yields of leaves were calculated. The roots were also collected and weighed. Dried leaves and roots were ground and analyzed. The primary results show significant differences among different cultivars.
