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Tuesday, September 24
 

12:14pm HST

Postharvest 1/Produce, Quality, Safety and Health Properties (PH 1/QUAL)
Tuesday September 24, 2024 12:14pm - 1:45pm HST
Identification of Genes Associated with the Chilling Prerequisites for Ripening in European Pear - Adwaita Parida
Effects of Cooling Delay and Harvest Maturity on ‘Gem’ Pear Internal Breakdown Development - Janet Turner
Efficacy of Ultraviolet Radiation for Control of the Mucor Rot Pathogen in Post-harvest Anjou and Bartlett Pears - Shawn McMurtrey
Preliminary Insights Into the Effects of Various Technologies of 1-methylcyclopropene (1-MCP) “AnsiP®” on Apple Fruit Quality During Shelf Life - Yosef Al Shoffe
Pre-Storage Ambient Temperature Delay Reduces Lenticel Blotch by Mediating Metabolic Alteration in Cold-Stored ‘Summer Prince’ Apples - Hyowon Park
1-Methylcyclopropene from HarvestHold Fresh® impact on ‘Red Delicious’ apple during cold storage - Ozge Horzum
Moderator
RB

Randolph Beaudry

Michigan State University
Dr. Randolph Beaudry, Professor, MSU Department of Horticulture (MSc, PhD, University of Georgia). His appointment is 50% MSU Extension and 50% AgBioResearch. His research program includes both regional and international projects and focuses on the physiology of plant responses to... Read More →
Tuesday September 24, 2024 12:14pm - 1:45pm HST
South Pacific 1

12:15pm HST

PH 1/QUAL - Identification of Genes Associated with the Chilling Prerequisites for Ripening in European Pear
Tuesday September 24, 2024 12:15pm - 12:25pm HST
The pear (Pyrus communis L.) has been classified as a climacteric fruit, undergoing a ripening process characterized by a transition from system-I to system-II ethylene production. This transition denotes a change in the regulation of ethylene biosynthesis from being auto-inhibitory to auto-stimulatory process. Increased ethylene production during climacteric ripening is accompanied by a corresponding rise in respiration. Pears require chilling for ripening, and the duration of chilling necessary for ripening varies among cultivars. ‘Bartlett
Speakers Co-authors
Tuesday September 24, 2024 12:15pm - 12:25pm HST
South Pacific 1

12:25pm HST

PH 1/QUAL - Effects of Cooling Delay and Harvest Maturity on ‘Gem’ Pear Internal Breakdown Development
Tuesday September 24, 2024 12:25pm - 12:35pm HST
‘Gem’ pear is a relatively new European pear cultivar with good fire blight resistance. Fruit can be eaten without ripening, either at harvest or upon removing from cold storage, when still crisp and juicy, or can be expected to ripen to a buttery melting texture after 30-60 days of regular-air (RA) storage (~30 °F). Although research initially indicated ‘Gem’ could be stored in RA conditions for 5 months with good quality retention, in practice ‘Gem’ storage has proved more challenging, primarily due to internal breakdown which can appear as early as 2 months after storage, either in RA or controlled atmosphere (CA) conditions. Additionally, with increasing commercial production of ‘Gem’, there is industry interest in extending storage beyond 5 months. This on-going study evaluates factors potentially influencing internal breakdown development, such as cooling delay (representing a delay of room temperature establishment at storage facility) and fruit maturity in year 1 and cooling delay and rate of controlled atmosphere (CA) establishment in year 2. In year 1, internal breakdown incidence was essentially absent for fruit harvested at 56 N flesh firmness but higher for fruit harvested at 44 N, especially where cooling was delayed. Fruit harvested at 56 N did not soften to below 18 N at 60 d storage, indicating a longer period of postharvest chilling is required for less mature fruit.
Speakers
JT

Janet Turner

Bio-Science Research Technician, USDA-ARS Hood River Worksite
Co-authors
KD

Kristal Dowell

USDA-ARS Hood River Worksite
NA
RL

Rachel Leisso

USDA-ARS Hood River Worksite
YD

Yu Dong

State Key Laboratory of Plateau Ecology and Agriculture
NA
Tuesday September 24, 2024 12:25pm - 12:35pm HST
South Pacific 1

12:35pm HST

PH 1/QUAL - Efficacy of Ultraviolet Radiation for Control of the Mucor Rot Pathogen in Post-harvest Anjou and Bartlett Pears
Tuesday September 24, 2024 12:35pm - 12:45pm HST
Mucor rot is caused by the pathogen Mucor piriformis, which can be responsible for severe post-harvest decay in a wide variety of commercially important fruit. There are a minimal number of fungicides that have been shown to be effective against Mucor rot. As a result, alternative methods for control of Mucor rot are currently being investigated. Ultraviolet light in the C spectrum (UV-C) is a germicidal form of radiation that has been demonstrated to kill common post-harvest pathogens. Little information is available for the effectiveness of UV-C light against M. piriformis. For this study, a Far-UV light (222-nm) and a UV-C light (265-nm) were tested for their ability to inactivate M. piriformis. The effects of three UV-C light treatments (0kJ, 10kJ, and 20kJ) were tested on Anjou and Bartlett pears that had been wound inoculated with M. piriformis. Lesion sizes were measured after five weeks in cold storage. The optimal dose required to inactivate M. piriformis in vitro in polystyrene wells with the Far-UV light was found to be 9 kJ, which, with available bulbs set at 8 cm distance, required 17 minutes of treatment. Results showed no significant effect of treatment on final lesion size for both the Far-UV light and the UV-C light. For both lights, final mean lesion sizes were larger for fruit that had been treated with the 20kJ and 10 kJ treatments when compared to the control fruit that received no UV-C treatment. Results from this study suggest that UV-C radiation in the 222-nm and 265-nm light spectrum have minimal impacts on reducing rot caused by M. piriformis in Anjou and Bartlett pears, and, given the duration of treatment required for spore deactivation, this technology may prove impractical for managing M. piriformis on other components of pear packing systems.
Speakers Co-authors
KW

Kevin Wang

USDA-ARS
NA
RL

Rachel Leisso

USDA-ARS Hood River Worksite
Tuesday September 24, 2024 12:35pm - 12:45pm HST
South Pacific 1

12:45pm HST

PH 1/QUAL - Preliminary Insights Into the Effects of Various Technologies of 1-methylcyclopropene (1-MCP) “AnsiP®” on Apple Fruit Quality During Shelf Life
Tuesday September 24, 2024 12:45pm - 12:55pm HST
Various postharvest formulations of 1-MCP have been innovated to optimize its efficacy for repeated use across storage, transportation, and retail stages. For small farms or fruits destined for farmers' markets, utilizing postharvest technologies with minimal 1-MCP concentrations is pivotal. In this trial, fruit from ‘Empire’, ‘Red Delicious’, and ‘RubyFrost’ apples was obtained from Cornell orchards in Lansing NY and Cornell AgriTech orchards in Geneva NY, on October 1, 4, and 13, respectively. Fruit were transported to the postharvest laboratory at Cornell University in Ithaca NY and fruit maturity indices were assessed. After cooling the fruit overnight, three different formulations of 1-MCP (tablet - AnsiP®-G, sticker - AnsiP®-Sticker, and sheet - AnsiP®-S, LYTONE Enterprise, Inc., Taiwan) were tested. The treatments were applied to fruit packed in cardboard boxes (30×30×48 cm), each containing 4 trays, at 3 °C for 24 hours. Fruit were then transferred to 20 ⁰C for 4 weeks, and fruit quality including internal ethylene concentration (IEC), flesh firmness, soluble solid content, titratable acidity, fruit color, IAD value index for peel chlorophyll content, and fruit weight were assessed weekly. Additionally, the release dynamics of 1-MCP from tablets, stickers, and sheets were evaluated in different trial. The 1-MCP tablets outperformed other treatments in reducing IEC and maintaining fruit firmness. The effects of different 1-MCP technologies on apple fruit quality and longevity during shelf life will be discussed.
Speakers
YA

Yosef Al Shoffe

Cornell Univeristy
Tuesday September 24, 2024 12:45pm - 12:55pm HST
South Pacific 1

12:55pm HST

PH 1/QUAL - Pre-Storage Ambient Temperature Delay Reduces Lenticel Blotch by Mediating Metabolic Alteration in Cold-Stored ‘Summer Prince’ Apples
Tuesday September 24, 2024 12:55pm - 1:05pm HST
Lenticel blotch as peel physiological disorder occurs in cold-stored apple fruit, characterized by irregular shape breakdown tissue without extending to the flesh. This study aimed to evaluate the postharvest treatment effects on fruit quality attributes, mineral nutrients, targeted metabolites, and incidence of lenticel blotch, and their relationship in cold stored ‘Summer Prince’ apple fruit. Fruits were harvested from 2 orchards, treated with postharvest 1-MCP, pre-storage ambient temperature delay for 7 days (ATC), and 1-MCP ATC at harvest and then stored at 0.5 °C for 5 months. There were significant differences in the organic matter content, nitrate nitrogen (NO3-N), and phosphoric acid contents in the soil. Calcium, manganese, copper, and zinc in leaf tissues were significantly different between 2 orchards. Lenticel blotch was effectively controlled by ATC treatment, while exacerbated by 1-MCP. Based on the results of VIP scores, histidine, valine, sucrose, K/Ca ratio, (K Mg P)/Ca ratio, total phenolic compounds, total flavonoids, ABTS, and DPPH were influenced by the postharvest treatments. In the results of volcano plot analysis, ATC treatment effectively controlled the symptoms and up-regulated in amino acids, such as valine, histidine, proline, and isoleucine. However, 1-MCP treatment caused to induce lenticel blotch by upregulating aspartic acid and glutamic acid. Therefore, the results indicated that pre-storage ambient temperature delay should control the lenticel blotch by mediating targeted metabolic alteration during cold storage.
Speakers
HP

Hyowon Park

Chung-Ang University
Co-authors
HP

Hnin Phyu Lwin

Washington State University
JK

Jeonghee Kim

National Institute of Horticultural and Herbal Science
NA
JL

Jinhee Lee

Chung-Ang University
NA
JL

Jinwook Lee

Chung-Ang University
NA
JP

Jongtaek Park

National Institute of Horticultural and Herbal Science
NA
SY

Seung Yeon Han

Chung-Ang University
NA
SK

Soon-il Kwon

National Institute of Horticultural and Herbal Science
NA
Tuesday September 24, 2024 12:55pm - 1:05pm HST
South Pacific 1

1:05pm HST

PH 1/QUAL - 1-Methylcyclopropene from differing sources: Impact on ‘Red Delicious’ apple during cold storage
Tuesday September 24, 2024 1:05pm - 1:15pm HST
Ethylene affects the postharvest quality and storage life by affecting the physiological changes related to ripening in apple fruits. 1-Methylcyclopropene (1-MCP), a cyclopropane-derived compound, is an effective ethylene inhibitor that blocks ethylene receptors at the cellular level, preventing ethylene effects and delaying its production. Several commercial products are available that provide controlled release of 1-MCP. In this study, a new 1-MCP technology was used, which uses a polymer sheeting material coated with cyclodextrin-encapsulated 1-MCP that is released when exposed to high humidity. Red Delicious apples were commercially harvested in Elbridge, Michigan, and 1-MCP sheets were applied targeting different 1-MCP concentrations (50, 100, 1000, 4000 ppb) and were placed on top of the fruit held in vented cardboard apple boxes in an open room. Another commercial 1-MCP product, Easyfresh®, powder (EF), (Fine Americas, Walnut Creek, CA) was applied at multiple dosages (0, 5, 50, 100, 1000 ppb) to fruit in cardboard apple boxes within sealed chambers as controls. Treated and untreated apples were stored in a cold room at 0 °C temperature and >90% relative humidity. We examined changes in ethylene production, respiration rate, firmness, soluble solids, titratable acidity, weight loss, and superficial scald during 7 months cold storage. The EF treatment was more effective than the 1-MCP sheet treatment for a given target concentration. The effect of 4000 ppb 1-MCP sheet was similar to the 50 ppb EF for the first 3 months of cold storage. The lack of effectiveness of the 1-MCP from sheet was likely due to escape of the 1-MCP from the apple box and off-target sorption by the cardboard.  EF 100 and 1000 ppb concentrations resulted in the lowest ethylene production, respiration rate, weight loss, and superficial scald values in the overall study for 6 months. These concentrations were also influential in preserving firmness, brix, and titratable acidity values. 1-MCP sheet efficacy could be improved by raising the dosage target or changing the architecture and the materials of the boxes in which treatment occurred.
Speakers
OH

Ozge Horzum

Ankara University
NA
Co-authors
RB

Randolph Beaudry

Michigan State University
Dr. Randolph Beaudry, Professor, MSU Department of Horticulture (MSc, PhD, University of Georgia). His appointment is 50% MSU Extension and 50% AgBioResearch. His research program includes both regional and international projects and focuses on the physiology of plant responses to... Read More →
Tuesday September 24, 2024 1:05pm - 1:15pm HST
South Pacific 1

1:15pm HST

PH 1/QUAL - Effects of Seed Sanitizing Treatments on Germination, Shoot Growth and Mineral Nutrient Composition of Four Microgreen Species
Tuesday September 24, 2024 1:15pm - 1:25pm HST
Microgreens are an emerging functional food that is sought after due to dense nutrient compositions as well as vibrant colors and textures. Seed contamination is one of the major food safety concerns as microgreens are consumed raw. Plant pathogenic diseases are also a concern as these can cause a reduction in the growth and quality of the crop. Seed sanitation methods should effectively reduce microbial load with minimal adverse effects on seed germination. The objective of this study was to investigate the effect of four seed sanitizing treatments on germination, shoot production and mineral nutrient concentrations of four microgreen species including chive (Allium schoenoprasum), shiso (Perilla frutescens var. crispa), scallion (Allium fistulosum) and dill (Anethum graveolens). Microgreen seeds were subject to four sanitizing treatments including: Tsunami 100 (400 ppm, 5 min), hydrogen peroxide (3%, 5 min), vinegar (1%, 15 min), and hot water (85°C, 10 sec). Seeds which were treated with deionized water for 10 min were considered to be the control. The microgreens were grown in a greenhouse and were planted into a peat-based substrate and a jute fiber mat in January 2024. Prior to greenhouse production, a germination test was conducted to investigate germination percentage of seeds for each species in response to the four sanitizing treatments or control. Microgreens were assessed for germination, shoot coverage, shoot height, fresh and dry shoot weight, and mineral nutrient concentrations. There was a significant interaction between microgreen species and the sanitizing treatment on fresh and dry shoot weight. The lowest fresh shoot weight for the three species chive, scallion and shiso was 938.2 g·m-2, 976 g·m-2, 907.8 g·m-2, respectively when treated with hot water, with the other three sanitizing treatments and control resulting in statistically similar fresh shoot weights. Dill microgreens showed little difference in fresh shoot weight among the five sanitizing treatments with values ranging from 506.4 g·m-2 in hot water to 868.2 g·m-2 in control. Sanitizing treatment and substrate type both had a significant effect on the shoot height of tested microgreens. Hot water treated microgreens produced the shortest shoots with a mean shoot height of 7.7 cm regardless of species or substrate type. The other four sanitizing treatments produced statistically similar shoot heights ranging from 8.01 cm with vinegar to 8.1 cm with Tsunami 100. The peat substrate increased overall shoot length in tested microgreens compared with jute fiber mats regardless of sanitizing treatment or species.
Speakers
JA

Jacob Arthur

Mississippi State University
Co-authors
GB

Guihong Bi

Mississippi State University
SW

Shecoya White

Mississippi State University
NA
TL

Tongyin Li

Mississippi State University
NA
ZC

Zonia Carvajal

Mississippi State University
NA
Tuesday September 24, 2024 1:15pm - 1:25pm HST
South Pacific 1

1:25pm HST

PH 1/QUAL - Towards Development of a Consumer-Preference Driven Digital Guide to Apple Fruit Cultivar Selection
Tuesday September 24, 2024 1:25pm - 1:35pm HST
There are over 7,500 apple varieties grown worldwide, each with its own set of organoleptic characteristics such as flavor, texture, and appearance. However, no more than 150 varieties have been introduced broadly in the global market. Consumer preference for apples is influenced by a complex interplay of factors beyond taste. The sheer variety of apples available in the market creates a valuable opportunity for a digital app that can help consumers navigate and select the best options based on their quality trait preferences. This project, in cooperation with U.S. Apple Association, aims to ultimately develop a digital app that will recommend apple varieties based on consumer’s preferences regarding sweetness, sourness, juiciness, crispiness, flavor, color, texture, and nutrition content. Thus, we examined the relationship between various sensory and physico-chemical data to understand their significance in apple selection. Five cultivars of apples grown organically (‘Ambrosia’, ‘Cosmic Crisp’, ‘Gala’, “Sugar Bee®’, and ‘Sweet Tango’) were purchased from a retail store in Mid Atlantic area during the winter season. Thirty apples of each cultivar (n=30) were measured for volume, weight, height, width, specific density, surface area, circumference, fizziness and for skin color (L*, a*, b*, hue angle, chroma). Firmness parameters and acoustic texture parameters were also measured. Additionally, total juice content, soluble solid content (SSC), titratable acidity and pH were assessed. Consumer panels (n=45: female=22, male=23) were conducted to evaluate traits including sweetness, sourness, flavor, texture/mouth feel, and overall eating quality (OEQ) using a five-point scale. Consumers displayed a stronger preference for ‘Cosmic Crisp’ and ‘Sugar Bee®’ varieties compared to ‘Gala’ and ‘Ambrosia’. This preference is driven by significantly higher consumer ratings for firmness, sweetness, and sourness of ‘Cosmic Crisp’ and ‘Sugar Bee®’. As highlighted in previous studies, correlations between consumer-rated sensory scores and their corresponding instrumental measurements were low. Furthermore, OEQ showed a stronger correlation with sensory ratings (r=0.54-0.84 ) than instrumental measurements (r=0.02 to 0.49). This suggests that consumer evaluation is critical, and may be a more reliable indicator, for the development of a digital app, compared to instrumental measurements. An accompanying consumer survey (n=30) indicated that crispness is a key factor considered by consumers when choosing apples. This project provided valuable insights and potential issues when developing a user-friendly app for consumers. It identified the dominant factors influencing apple selection and showed methods to cross-validate sensory ratings with corresponding instrumental measurements.
Speakers
EP

Eunhee Park

USDA-ARS
NA
Co-authors
BZ

Bin Zhou

USDA-ARS
NA
CG

Christopher Gerlach

US Apple Association
NA
JF

Jorge Fonseca

USDA-ARS
NA
RO

Regina O'Brien

United States Department of Agriculture
NA
VG

Verneta Gaskins

USDA-ARS, Beltsville Agricultural Research Center
WJ

Wayne Jurick

USDA-ARS
NA
Tuesday September 24, 2024 1:25pm - 1:35pm HST
South Pacific 1

1:35pm HST

PH 1/QUAL - Evaluating Fresh-cut Lettuce Quality via Image Analysis
Tuesday September 24, 2024 1:35pm - 1:45pm HST
Visual quality is an important factor for consumer purchasing decisions of fresh-cut lettuce. Consumer behavior towards produce quality has been studied via traditional human evaluations. For sensory studies, quality evaluations are commonly done by trained human panels and consumer panels. This study was to investigate the possibility of replacing human evaluation with a machine based approach, using image capturing and analysis, and determine whether efficiency of produce quality analysis can be enhanced. Three types of data were collected: (a) consumers’(n=200) evaluation of lettuce on the picture, (b) instrumental analysis of samples (package head-space gas composition (O2, CO2) and electrolyte leakage), (c) image analysis of lettuce on the pictures. For image analysis, ImagePro’s smart segment tool was used to classify the lettuce samples into five regions: adult leaf, baby leaf, rib, rib degradation, and leaf degradation. This was used to find and calculate L*a*b, hue angle, chroma values, area, and relative area of these regions. Lettuce samples consisted of four cultivars (Green Forest, King Henry, Parris Island Cos, PI 491224). Samples were measured on day 7, 10, and 13 of storage. To predict browning score, data sets (b) and (c) were fed into a regression algorithm. The scores assigned by trained panels served as the target variables. The results showed a strong correlation between consumer’s browning score on the pictures and predicted scores generated by the regression model (r=0.74). Interestingly, removing the instrumental data set (b) did not worsen the model’s performance. The model achieved an R2 of 0.92 and RASE of 8.90 when using trained data sets (a) and (c), and an R2 of 0.91 and RASE of 8.53 when using trained data set (c) only. While a correlation coefficient of 0.74 indicates a promising relationship between image analysis and human evaluation of browning score, it’s not sufficient to definitively replace human evaluation. Further studies with larger datasets and exploration of more advanced machine learning models could lead to a more robust statistical model.
Speakers
EP

Eunhee Park

USDA-ARS
NA
Co-authors
EE

Ella Evensen

USDA-ARS
NA
IS

Ivan Simko

USDA-ARS
NA
JF

Jorge Fonseca

USDA-ARS
NA
YL

Yaguang Luo

USDA/ARS
NA
Tuesday September 24, 2024 1:35pm - 1:45pm HST
South Pacific 1

2:14pm HST

Postharvest 1 (PH 1)
Tuesday September 24, 2024 2:14pm - 4:00pm HST
Ethylene Degreening: A Technique to Enhance Peel Color of Georgia-Grown Satsuma (Citrus reticulata) Citrus Fruit - Taiwo Owolanke
Postharvest Storage Temperatures to Promote Anthocyanin Accumulation and Antioxidant Activity in Blood Orange cv. Moro - Fariborz Habibi
Preserving Flavor in Grapefruit Juice: Continuous Flow High-Pressure Homogenization Versus Conventional Treatment - Jayashan Adhikari
Effects of Alternative Atmosphere Storage on the Postharvest Quality of Georgia-grown Blackberries - Ramsey Corn
Examining the Effect of Storage Temperatures on Chilling Injury Incidence of Georgia-Grown Peaches - Orestis Giannopoulos
Postharvest melatonin application maintains quality of jackfruit bulbs by alleviating enzymatic browning and oxidative stress under low temperature storage - Jashanpreet Kaur
Catalysis of 1-Methylcyclopropene Degradation By Non-Target Materials: Metals and Metal Salts - Ozge Horzum


Moderator
TO

Taiwo Owolanke

Graduate Research Assistant, University of Georgia
Tuesday September 24, 2024 2:14pm - 4:00pm HST
South Pacific 2

2:15pm HST

PH 1 - Ethylene Degreening: A Technique to Enhance Peel Color of Georgia-Grown Satsuma (Citrus reticulata) Citrus Fruit
Tuesday September 24, 2024 2:15pm - 2:30pm HST
In recent years, the production of Satsuma oranges (Citrus reticulata) has significantly increased in the state of Georgia. Satsumas are known to develop a deep orange peel color naturally when the temperature drops below 12°C at night. However, due to the subtropical climate of South Georgia, the fruit often fails to turn orange naturally even after attaining physiological maturity standards. Consumers tend to choose fruits based on appearance, with brightly colored oranges being more likely to attract buyers. Degreening is a postharvest technique that utilizes gaseous ethylene (C2H4) to accelerate and promote the development of orange/yellow color pigments in fresh-market citrus fruit. The study was conducted using four Satsuma cultivars ('Brown Select', 'Owari', 'Miho', and 'Xie Shan') harvested at physiological maturity yet with green peel coloration. The fruit samples were subjected to a degreening treatment which involved exposure to a continuous flow of
Speakers
TO

Taiwo Owolanke

Graduate Research Assistant, University of Georgia
Co-authors
AD

Angelos Deltsidis

University of Georgia
AB

Anthony Bateman

University of Georgia
NA
JP

Jake Price

University of Georgia, Extension
NA
OG

Orestis Giannopoulos

University of Georgia
RC

Ramsey Corn

University of Georgia
Tuesday September 24, 2024 2:15pm - 2:30pm HST
South Pacific 2

2:30pm HST

PH 1 - Postharvest Storage Temperatures to Promote Anthocyanin Accumulation and Antioxidant Activity in Blood Orange cv. Moro
Tuesday September 24, 2024 2:30pm - 2:45pm HST
Blood oranges (Citrus sinensis L. Osbeck) fruit contain valuable compounds for human health, including anthocyanins, flavonoids, polyphenols, hydroxycinnamic acids, and ascorbic acid. Anthocyanin is considered an important internal quality index of blood oranges due to its red color and antioxidant activity. Blood orange pigmentation under similar growing conditions depends on some factors including cultivar, cultural practices, soil characteristics, climate conditions, maturity, and harvest maturity. Blood orange fruit require cold temperatures between 8 °C to 15 °C during the last ripening stages to develop high levels of anthocyanin in their flesh. However, commercial production of blood oranges in subtropical or tropical regions is limited due to very low or lack of cold temperatures to enhance anthocyanin concentration in fruit. We evaluated the effect of different storage temperatures (10, 15, and 20 °C) on anthocyanin enhancement and the antioxidant activity of ‘Moro’ blood orange for 42 days. Fruit were harvested from a commercial citrus orchard in south Georgia and transported to the postharvest lab in Gainesville, Florida. Fruit were checked for absence of defects andr rind injuries, sanitized with 100 ppm sodium hypochlorite solution, and superficial water removed from the fruit surface. The fruit were then divided into sets of four replicates of 10 fruit per treatment and placed in mesh bags for storage at 10, 15, or 20 °C with 90 % relative humidity. Anthocyanin accumulation and antioxidant activity in the flesh were evaluated every 14 days for 42 days. There were significant differences among the applied storage temperatures for anthocyanin content and antioxidant activity. The highest anthocyanin concentration and antioxidant activity were observed at 10 °C, while the lowest anthocyanin level was at 20 °C for all sampling times. The efficiency of these temperatures in enhancing flesh anthocyanin and antioxidant activity was in the following order: 10 °C > 15 °C > 20 °C at all sampling times. Overall, it can be concluded that cold storage can be used as a simple technology for enhancing bioactive compounds and antioxidant activity in blood oranges that are poorly pigmented at harvest in subtropical or tropical climates like Florida.
Speakers
FH

Fariborz Habibi

University of Florida
Co-authors
AS

Ali Sarkhosh

University of Florida
NA
JB

Jeffrey Brecht

University of Florida
NA
Tuesday September 24, 2024 2:30pm - 2:45pm HST
South Pacific 2

2:45pm HST

PH 1 - Preserving Flavor in Grapefruit Juice: Continuous Flow High-Pressure Homogenization Versus Conventional Treatment
Tuesday September 24, 2024 2:45pm - 3:00pm HST
Grapefruit is known for its citrus aroma and tangy flavor. Processors use post-harvest preservation methods for juice production to maintain quality during storage for market distribution. These processes aim to preserve quality and safety while reducing unwanted compounds such as furanocoumarins. However, traditional methods like thermal pasteurization alter sensory quality and nutrient contents. With consumers demanding fresh-like taste without safety issues, researchers have aimed to develop new processing technologies. Here, we tested continuous flow high-pressure homogenization (CFHPH) for grapefruit juice processing. This method may extend shelf-life while maintaining fresh-like flavor. In this study, CFHPH was applied at various pressure levels (200, 250, 300 MPa), inlet temperatures (4 or 22 °C), and a flow rate of 1.125 L/min, and compared with conventional high-temperature short time (HTST) processing for preserving flavor compounds in Ruby Red grapefruit juice during storage at 4 °C for 45 days. Gas chromatography–mass spectrometry analysis identified key volatile compounds such as limonene, myrcene, α-pinene, β-pinene, and linalool. CFHPH preserved flavor compounds better than HTST, while HTST treatments led to flavor loss and off-notes. CFHPH maintained major volatiles like limonene, suggesting its potential as a consumer-preferred preservation method. This research underscores the significance of innovative techniques for maintaining grapefruit juice sensory quality, which is essential for customer satisfaction and market success.
Speakers Co-authors
KA

Koushik Adhikari

University of Georgia
NA
RS

Rakesh Singh

University of Georgia
NA
Tuesday September 24, 2024 2:45pm - 3:00pm HST
South Pacific 2

3:00pm HST

PH 1 - Effects of Alternative Atmosphere Storage on the Postharvest Quality of Georgia-grown Blackberries
Tuesday September 24, 2024 3:00pm - 3:15pm HST
Blackberries have several susceptibilities that contribute to the fruit’s high perishability resulting in a short shelf-life. The industry-standard technique for maintaining the postharvest quality is to store berries in a room with low temperature and high relative humidity to slow senescence. Despite the use of cold storage, the shelf-life of fresh-market blackberries can be short, limiting their marketing potential. The application of innovative technologies such as controlled atmospheres (CA), or the addition of gaseous ozone (O3) to the cold storage rooms could aid in maintaining the postharvest quality. During the 2023 blackberry season, three harvests were conducted paired with a sensory survey one day after. Four blackberry cultivars (Caddo, Ouachita, Ponca, Osage) were hand-picked, field-packed, and hand-sorted before being placed into cold storage. The storage conditions included a control of cold storage (1 °C, 95% RH), cold storage with additional controlled atmosphere (10% CO2 10% O2), and cold storage with gaseous ozone (0.5 ppm). The blackberries were kept under the above storage conditions for up to 21 days, with quality assessments occurring every seventh day. Quality assessments included sensory attributes of visual quality, weight loss, color, firmness, respiration rates, red drupelet reversion (RDR) presence, anthocyanin content, titratable acidity, and total soluble solids. Significant differences between treatments were recorded in fruit firmness of Caddo and Ouachita berries after seven days of storage. The CA and ozone treatments resulted in higher firmness of Caddo berries compared to the control. The CA and ozone treatments resulting in lower firmness of Ouachita berries compared to the control. RDR was separated by presence of RDR with the parameters none, low, and high. In each harvest, Ouachita had the highest presence of RDR after harvest compared to the other cultivars. Caddo exhibited low to no RDR in each harvest initially and after storage. Caddo was favored by panelists in the sensory survey related to the overall flavor and overall appearance. The experiment will be repeated during the 2024 blackberry season to evaluate the above cultivars’ aroma volatile compound levels using a GC/MS system.
Speakers
RC

Ramsey Corn

University of Georgia
Co-authors
AD

Angelos Deltsidis

University of Georgia
OG

Orestis Giannopoulos

University of Georgia
ZR

Zilfina Rubio

University of Georgia
Tuesday September 24, 2024 3:00pm - 3:15pm HST
South Pacific 2

3:15pm HST

PH 1 - Examining the Effect of Storage Temperatures on Chilling Injury Incidence of Georgia-Grown Peaches
Tuesday September 24, 2024 3:15pm - 3:30pm HST
Peaches are climacteric fruit that can continue ripening after harvest. When stored at elevated temperatures, peaches exhibit higher respiration rates and increased ethylene production, which makes low-temperature storage key for quality preservation. Cold storage slows down metabolic activities responsible for senescence. However, temperatures between 36 and 46°F (2.2-7.7°C) might induce a disorder commonly known as chilling injury, hence, this range has been named by many scientists as the “killing zone”. The disorder is detrimental to peach quality as it affects the flesh texture, appearance, and fruit juiciness overall. Depending on the severity, peaches might not show external symptoms of the disorder which usually appears after transfer to ambient conditions. Factors such as varietal differences, storage durations, and growing conditions may influence the severity of chilling injury symptoms. Very little research has been done in the Southeast regarding chilling injury incidence, while many of the newer cultivars have never been investigated. This project aimed to investigate the appearance of chilling injury symptoms in important peach cultivars grown in the Southeastern United States. Peaches were stored at five different temperatures of 33, 36, 41, 46, and 50°F (0.5, 2.2, 5, 7.7, and 10°C) and 95% relative humidity for up to four weeks. Quality evaluations occurred on days 0, 7, 14, 21, and 28 after harvest, which were followed by three days of ambient storage to allow for the development of chilling injury. Quality measurements included weight loss, decay incidence, total soluble solids, titratable acidity, firmness, as well as visual appearance. Results show that 33°F storage temperatures had lower respiration. Higher storage temperatures of 41, 46, and 50°F resulted in increased weight loss and lower firmness readings. Peaches stored at 33 and 36°F were juicier until the 21st day of storage. All tested temperatures were tolerated for a storage period of 7 days, with no visible symptoms. On the other hand, storage durations longer than 14 days at temperatures of 36, 41, and 46°F showed the highest chilling injury incidence. More research is currently underway in order to create a map of chilling injury tolerance across different cultivars that are important for the Southeastern region.
Speakers
avatar for Orestis Giannopoulos

Orestis Giannopoulos

University of Georgia
Co-authors
AD

Angelos Deltsidis

University of Georgia
DC

Dario Chavez

University of Georgia
NA
RC

Ramsey Corn

University of Georgia
Tuesday September 24, 2024 3:15pm - 3:30pm HST
South Pacific 2

3:30pm HST

PH 1 - Postharvest melatonin application maintains quality of jackfruit bulbs by alleviating enzymatic browning and oxidative stress under low temperature storage
Tuesday September 24, 2024 3:30pm - 3:45pm HST
Jackfruit bulbs are susceptible to postharvest browning which is a major factor limiting shelf life and marketability. In this investigation, the impact of postharvest melatonin (MLT) application on enzymatic browning and antioxidant metabolism in jackfruit bulbs was evaluated. Jackfruit bulbs were dipped in different concentrations of MLT (0, 0.05, 0.1 and 0.2 mmol L-1) prior to cold storage at 4 ± 1 °C temperature and 85-90% relative humidity over the duration of 20d. MLT application considerably delayed the process of senescence as demonstrated by a reduction in browning index and softening of bulbs with 0.1 and 0.2 mmol L-1 MLT application, respectively. At the end of the storage period, 0.2 mmol L-1 MLT treatment significantly maintained higher ascorbic acid (46.9 %), total phenolics (22.3%) and total carotenoids (26.3%) compared to control. Whilst 0.1 mmol L-1 MLT application curtailed fruit weight loss (46.0%) and improved total flavonoid content (16.7 %) and DPPH radical scavenging activity (12.5%) as compared to the control. The levels of polyphenol oxidase and guaiacol peroxidase were significantly lower in 0.1 mmol L-1 and 0.2 mmol L-1 MLT treated bulbs, respectively. The activity of antioxidant enzymes including catalase, superoxide dismutase, and ascorbate peroxidase was improved with the application of 0.2 mmol L-1 MLT. In addition, all MLT treatments effectively reduced malondialdehyde, lipoxygenase and hydrogen peroxide radicals. These findings suggest that dip treatment of MLT (0.1 and 0.2 mmol L-1) effectively lessen flesh browning along with maintenance of antioxidant potential and postharvest quality of jackfruit bulbs.
Speakers
JK

Jashanpreet Kaur

PhD Scholar, Edith Cowan University
Co-authors
AW

Andrew Woodward

Edith Cowan University, Joondalup, Western Australia, Australia
NA
EA

Eben Afrifa-Yamoah

Edith Cowan University, Joondalup, Western Australia, Australia
NA
HM

Hafiz Muhammad Shoaib Shah

Edith Cowan University, Joondalup, Western Australia, Australia
NA
MS

Muhammad Sohail Mazhar

Department of Industry, Tourism and Trade Northern Territory
NA
ZS

Zora Singh

Edith Cowan University
Tuesday September 24, 2024 3:30pm - 3:45pm HST
South Pacific 2

3:45pm HST

PH 1 - Catalysis of 1-Methylcyclopropene Degradation By Non-Target Materials: Metals and Metal Salts
Tuesday September 24, 2024 3:45pm - 4:00pm HST
1-Methylcyclopropene is a cyclic olefin that inhibits ethylene action and is released as a gas from a formulated cyclodextrin. Different concentrations of 1-MCP are used globally in the fruit industry to enhance the preservation of quality attributes. The concentrations applied cannot be considered stable due to losses to target and non-target sites within the storage or treatment room environment. Copper is used in cooling systems because it transfers heat more efficiently than many other materials, but it is also used as a catalyst for chemical modification. So, we investigated the absorption or degradation of 1-MCP by various metals, including copper. These forms included metal bars (hot and cold-rolled steel, aluminum, galvanized metal, silver, gold, stainless steel, and brass), copper pipes (polished, oxidized, and with patina) and copper salts [covellite (CuS, copper II sulfide); eriochalcite (CuCl22H2O, copper II chloride); chalcopyrite (CuFeS2, copper iron sulfide); cupric carbonate (CuCO3, copper II carbonate); chalcocite (Cu2S, copper I sulfide), cuprite (Cu2O, copper I oxide); chalcanthite (CuSO45H2O, copper sulfate); tenorite (CuO, copper II oxide), and cupric acetate (Cu(CH3CO2)2, copper II acetate)], which possessed copper atoms at different levels of oxidation. The metal pieces had a surface area of 435 cm2, and the powders weighed 1 g. We put the materials in 480-mL glass jars and added 50 μL L−1 of 1-MCP gas to the headspace. Gas concentrations were measured at 0, 2, 4, 6, and 24 h in both humid and dry conditions at room temperature. The loss of 1-MCP was more pronounced in humid condition than in dry condition except when exposed to cupric carbonate. While covellite, eriochalcite, and chalcopyrite caused a 1-MCP loss of over 90% within 24 h in both conditions, stainless steel, aluminum, galvanized iron mesh, silver and galvanized iron yielded a 1-MCP loss below 10%. On the other hand, the impact of copper pipes on reducing 1-MCP is evident, and the reduction of 1-MCP also increases as the oxidation level of the copper increases. Based on mass spectral analysis of the headspace in the treatment chambers, the decrease of 1-MCP appears to occur by adsorption by the materials, polymerization, and disintegration into break-down products. The implications for 1-MCP reductions in commercial treatment rooms will be discussed.
Speakers
OH

Ozge Horzum

Ankara University
NA
Co-authors
NS

Nobuko Sugimoto

Michigan State University
NA
PE

Philip Engelgau

Michigan State University
NA
RB

Randolph Beaudry

Michigan State University
Dr. Randolph Beaudry, Professor, MSU Department of Horticulture (MSc, PhD, University of Georgia). His appointment is 50% MSU Extension and 50% AgBioResearch. His research program includes both regional and international projects and focuses on the physiology of plant responses to... Read More →
Tuesday September 24, 2024 3:45pm - 4:00pm HST
South Pacific 2
 
Wednesday, September 25
 

9:59am HST

Postharvest 2 (PH 2)
Wednesday September 25, 2024 9:59am - 12:30pm HST
Postharvest Performance of Tomato Fruit at Shelf-life Conditions in Relation to Fertilization Rate During Plant Production and Fruit Ripening Stage at Harvest - Sotirios Tasioulas
Identification of NAC Transcription Factors Involved in the Ripening and Senescence of Pepper (Capsicum annuum L.) Fruit - Kyeonglim Min
Comparative Analysis of Volatile Organic Compounds of Eight Microgreens in the Brassicaceae Family - Jinhe Bai
Nutritional Quality and Shelf-Life of “Living Lettuce” Through 28 Days of Cold Storage - Marlee Trandel Hayse
Comparing the Postharvest Phytonutrient Content of Red and Green Butterhead Lettuce Cultivars - Marlee Trandel Hayse
Production System Alters Phytonutrient Content of ‘Skyphos’ Butterhead Lettuce - Marlee Trandel Hayse
Improving Postharvest Storage and Late-Season Production of Caladium Tubers in Florida Through Chemical Treatments - Gasselle Cordova
Impact of Storage Environment on Dormancy Progression and Microbiome of Potato Tubers - Munevver Dogramaci
Strawberry Anthocyanins, Extraction, Assessments and Storage - Toktam Taghavi
Isolation of Pathogenic Strain of Trichoderma atroviride from Symptomless, Disease-Free Sweetpotato (Ipomoea batatas L.) Storage Roots - Richard Noel Torres

Moderator
MD

Munevver Dogramaci

United States Department of Agriculture
Wednesday September 25, 2024 9:59am - 12:30pm HST
Nautilus 1

10:00am HST

PH 2 - Postharvest Performance of Tomato Fruit at Shelf-life Conditions in Relation to Fertilization Rate During Plant Producti
Wednesday September 25, 2024 10:00am - 10:15am HST
This study investigates how the ripening stage at harvest and fertilization rate impact tomato fruit quality. Tomato plants (cv. HM1823) were grown during the Fall 2023 season under four levels of fertilization (75%, 100%, 125%, and 150% of the recommended UF/IFAS rate, which is 200 lb/A nitrogen and potassium). Fruit were harvested at four distinct ripening stages: mature green (MG), turning (T), pink (P), and red (R), as assessed visually and stored at 25°C. Color, firmness, soluble solids content (SSC), pH, and titratable acidity (TA) were measured every 4 days until 12 or 16 days (for MG fruit). According to the analysis of variance, all factors (fertilization rate-FR, ripening stage at harvest- RS and shelf life period- SL), as well as the interaction RSSL significantly (P
Speakers
ST

Sotirios Tasioulas

SWFREC - University of Florida/IFAS
Co-authors
JW

Jessie Watson

SWFREC - University of Florida/IFAS
NA
PT

Pavlos Tsouvaltzis

Southwest Florida Research and Education Center, University of Florida
NA
Wednesday September 25, 2024 10:00am - 10:15am HST
Nautilus 1

10:15am HST

PH 2 - Identification of NAC Transcription Factors Involved in the Ripening and Senescence of Pepper (Capsicum annuum L.) Fruit
Wednesday September 25, 2024 10:15am - 10:30am HST
Pepper (Capsicum annuum L.) as a non-climacteric fruit is usually harvested at either mature green or ripe stage, and fruit quality including texture, color, and nutritive values is different according to the fruit developmental stages. Understanding molecular mechanisms of fruit ripening and senescence processes is crucial to control the fruit quality and reduce the postharvest loss. This study was aimed to identify NAC transcription factors (TFs) involved in the ripening and senescence of pepper fruit by analyzing gene expressions and protein-protein interactions. To identify NAC genes related to the ripening, transcript levels of total 104 NAC TFs were investigated using publicly available transcriptome data. Among them, transcript levels of 8 genes significantly increased during the ripening and senescence. Their phylogenetic analysis also showed that they are closely associated with NAC TFs which play a role in ripening or senescence in other crop species. Among them, 4 genes showing abundant transcript levels at the ripening stage were selected, including CaNAC14, 45, 84, and 92, to further investigate their roles. In pepper (C. annuum var. ‘Gyeonggiyangpyeiong’) fruit, their expressions clearly increased during the ripening or induced by exogenous ABA, the major phytohormone regulating non-climacteric fruit ripening. To reveal a relationship between the CaNACs and ABA signaling, protein-protein interactions between the CaNACs and ABA receptors were analyzed through yeast- two-hybrid and bimolecular-fluorescence complementation assays. Among them, CaNAC92 and CaPYL12, the ABA receptor, interacted each other in a nucleus with an ABA-dependent manner. This result indicated that their interaction may affect the ABA signaling or the transcriptional regulation during the ripening. We identified CaNAC TFs involved in the pepper fruit ripening and suggested CaNAC92 as a putative molecule functioning in the ripening. These findings will provide genetic information to control the ripening and to improve pepper fruit quality.
Speakers
KM

Kyeonglim Min

Student, Seoul National University
Co-authors
EJ

Eun Jin Lee

Seoul National University
Wednesday September 25, 2024 10:15am - 10:30am HST
Nautilus 1

10:15am HST

Workshop: Breeding for Post-harvest Shelf Life in Vegetable Crops
Wednesday September 25, 2024 10:15am - 12:15pm HST
Improved postharvest storage is a major target for vegetable-crop production. Nutritional contents and flavor are traits highly affected from harvesting to final consumption. Several reports have demonstrated that these traits are strong driving factors impacting consumers’ decisions and preferences when purchasing food. Therefore, these are economically important traits related to strong commercialization potential and improved market value. Assessing the potential variation in post-harvest shelf life among vegetable crops will be critical to identify parental lines that can be used in breeding programs to improve these traits and their effect on the quality of produce. Additionally, investigating the genetic mechanisms underlying post-harvest shelf life in horticultural crops is critical. This will result in the development of molecular breeding tools associated with improving shelf life, which will accelerate breeding. This interest group seeks to provide case studies from experts worldwide who have worked on breeding for nutritional contents, flavor, and post-harvest shelf life in vegetables.

Coordinator(s)
  • Carlos Avila, Texas A&M AgriLife Research, Weslaco, TX, United States
Moderator(s)
  • Dennis Nicuh Lozada, New Mexico State University, Plant and Environmental Sciences, Las Cruces, NM, United States
  • Devi Kandel, Langston University, United States
Speaker/Participant(s)
  • Carmit Ziv, Institute of Postharvest and Food Science, ARO, Volcani Institute, Israel
    Breeding for improved postharvest quality of peppers during cold storage (15 mins)
    Summary: Dr. Ziv's research program studies postharvest disease control of fruity vegetables by developing environment-friendly treatments to control postharvest fungal rot, understanding resistance mechanisms of phytopathogenic fungi to cold storage, uncovering the role of lipids in determining the interactions between fruits and pathogenic fungi during storage.
  • Carlos Avila, Texas A&M AgriLife Research, Weslaco, TX, United States
    Lack of locule gel increases post-harvest shelf-life and quality in tomato (15 mins)
    Summary: Dr. Avila's research program is focused on the development of tomato and spinach cultivars adapted to Texas conditions. Research work includes the characterization of plant a/biotic stress and vegetable quality trait regulation using molecular, physiological, and biochemical approaches to develop applied strategies to improve breeding efficiency and ultimately crop resistance and quality. Current efforts include the development of firm long shelf-life tomato cultivars with improved flavor and nutritional content.
  • Bhimu Patil, Texas A&M University, Vegetable and Fruit Improvement Center, College Station, Texas, United States
    Post-harvest shelf life and nutritional quality in horticultural crops (15 mins)
    Summary: Dr. Patil has been actively involved in both research and educational activities related to global level ‘Foods for Health’. His research, in collaboration with trans-disciplinary scientists, related to bioactive-derived assays led to isolation and characterization of certain bioactive compounds in citrus and turmeric and other vegetables. His research is also focused on pre and postharvest effects on bioactive compounds in citrus, onion and recently his work is focused on other vegetables and fruits.
  • Alexander Goldshmidt, Institute of Plant Sciences, ARO, Volcani Institute, Israel
    Exploring the Role of CLASS-II KNOX mediated Ripening Pathways for the Discovery of Novel Genetic Approaches to Extend Fruit Shelf Life (15 mins)
    Summary: Dr. Goldshmidt research is focused on investigation of the genetic factors and networks controlling development of the pericarp tissues in fruits of Solanaceae crops tomato and pepper. His lab seek to use their research to develop novel genetic solutions and tools to extend fruit's shelf life and improve its consumer attractiveness and nutritional values.
  • Barbara Blanco Ulate, UC Davis, United States
    Control of fruit ripening, improvement of fruit quality, and fruit-pathogen interactions. (15 mins)
    Summary: Dr. Blanco-Ulate's research program integrate systems biology approaches with biochemical and physiological analyses to study fruit development and quality traits, and to establish a novel framework for the early detection and efficient management of fruit diseases.





Wednesday September 25, 2024 10:15am - 12:15pm HST
Coral 2

10:30am HST

PH 2 - Comparative Analysis of Volatile Organic Compounds of Eight Microgreens in the Brassicaceae Family
Wednesday September 25, 2024 10:30am - 10:45am HST
This study investigates the volatile organic compounds (VOCs) of eight microgreens in the Brassicaceae family, known for their robust flavors and potential health benefits, including detoxification properties and anti-cancer effects. The microgreens analyzed included five Brassica species—B. juncea (mustard), B. napus (kale), B. rapa (mizuna), B. oleracea L. var. capitata (red cabbage), and B. oleracea L. var. italica (broccoli)—as well as Eruca sativa (arugula), Lepidium sativum (cress), and Raphanus sativus (radish). The above-ground plant tissues were homogenized in saturated salt water and the VOCs were examined using headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GCMS). A total of 117 VOCs were identified across all tested species, with individual species VOC counts ranging from 42 to 67. Radish showed the highest total ion current at 676 million (mTIC), with other species varying down to 190 mTIC in arugula. Notably, sulfur and/or nitrogen-containing compounds (SCCs and/or NCCs) constituted over 90% of the total VOCs collected from all species, with Brassica species containing more than 96% SCCs and 93% NCCs. Conversely, radish and arugula had less than 88% NCCs, and cress contained only 66% SCCs. Dominant compounds were primarily isothiocyanates, with significant variability in major VOCs across different species. For instance, allyl isothiocyanate was prevalent in mustard, mizuna, and red cabbage; 4-isothiocyanato-1-butene in kale and mizuna; isothiocyanatomethyl-benzene in cress; 1-isothiocyanato-4-(methylthio)-butane in arugula and broccoli; and (E)-4-isothiocyanato-1-(methylthio)-1-butene in radish. The reduced SCCs in cress were attributed to the higher presence of benzyl nitrile and benzyl isocyanate, while the lower NCCs in radish were linked to increased levels of dimethyl disulfide and dimethyl trisulfide. This diversity in SCCs and NCCs contributes to the varied volatile flavors and potential health benefits of these microgreens.
Speakers
JB

Jinhe Bai

USDA-ARS
Co-authors
AP

Anne Plotto

USDA-ARS
NA
ER

Erin Rosskopf

USDA-ARS
NA
FD

Francesco Di Gioia

Pennsylvania State University
JH

Jason Hong

USDA Agricultural Research Service
NA
LW

Libin Wang

Nanjing Agricultural University,
NA
WZ

Wei Zhao

USDA-ARS
NA
Wednesday September 25, 2024 10:30am - 10:45am HST
Nautilus 1

10:45am HST

PH 2 - Nutritional Quality and Shelf-Life of “Living Lettuce” Through 28 Days of Cold Storage
Wednesday September 25, 2024 10:45am - 11:00am HST
Lettuce (Lactuca sativa) is cultivated in controlled environment agriculture via vertical production or hydroponic well systems. To extend shelf-life, growers harvest “living lettuce” by keeping roots intact, but there is limited data supporting this harvesting technique. The objective of this study was to assess the use of clamshell and root treatments on the shelf-life and nutritional quality of lettuce. Treatments included storage either inside or outside of plastic clamshells and with roots removed or intact. Lettuce was stored for 28 days at 4 oC, 85% relative humidity. Percent weight loss (%weight loss), visual ratings (1 to 5 scale, 1 = poor and 5 = excellent), and colorimeter analysis (L*, a*, b*, c*, hue angle) were measured every 3 days. Total assays of chlorophyll, carotenoids, anthocyanins, flavonoids and phenolic activity were analyzed via UV VIS spectrophotometry every 7 days throughout storage. Few differences were seen in root treatment. As days in storage progressed, %weight loss progressed to 16.9%. Closed clamshells decreased %weight loss (5.5%) compared to open (18.1%). Visual ratings of yellowing/nutrient deficiency, relative greenness, wilt/head looseness, and salability decreased through storage. Lettuce stored in closed clamshells decreased %weight loss by 12% and delayed wilt/head looseness by 11 days. Similarly, lettuce stored in closed clamshells with roots were salable until 18 days in storage, while open clamshells were no longer salable after 7 days. Short root length (~4 cm) may be the dominant factor for the lack of significance as previous research suggested harvesting “living lettuce” with 9 cm roots increased storage and quality. Color data differed, and lettuce stored in closed clamshells with roots had more intense red color a* (-3.7 vs. -2.7), blue color b* (12.6 vs 10.1), and were purer in color c* (13.3 vs. 10.5) compared to open clamshells with roots. No differences were seen in total anthocyanin (mg/L), total chlorophyll (g/g) or total carotenoid (g/g) content in clamshell or root treatment (p > 0.05). Through 14 days of storage, total anthocyanins degraded by 7.55 mg/L (Day 0 = 10.78 vs. Day 14 = 3.22), while no loss of total chlorophyll (9204.84 g/g) or total carotenoids (1947.25 g/g) were seen. Anthocyanins are water soluble pigments and with increased weight/water loss, anthocyanins solubilize leading to degradation. Chlorophylls and carotenoids were not lost, potentially due to low enzyme activity of chlorophyllase. This experiment indicates consumers should eat lettuce within 7-10 days of harvest for highest nutritional and postharvest quality.
Speakers
MT

Marlee Trandel Hayse

Auburn University
Co-authors
CR

Camila Rodrigues

Auburn Univeristy
NA
CB

Caroline Blanchard

Auburn University
DW

Daniel Wells

Auburn University
NA
MH

MD Hasibur Rahman

Auburn University
NA
TR

Tanzeel Rehman

Auburn University
NA
Wednesday September 25, 2024 10:45am - 11:00am HST
Nautilus 1

11:00am HST

PH 2 - Comparing the Postharvest Phytonutrient Content of Red and Green Butterhead Lettuce Cultivars
Wednesday September 25, 2024 11:00am - 11:15am HST
Lettuce is a popular leafy vegetable that can play an important role in human nutrition and diets with regular consumption. Lettuce cultivars can vary considerably in their postharvest nutritional composition. It is critically important to determine which lettuce cultivars have the highest nutritional quality to allow growers to select those that are best for their market. The objectives of this experiment were twofold, to determine differences in the phytonutrient content of two butterhead lettuce cultivars, ‘Nancy’ (green butterhead) and ‘Skyphos’ (red butterhead), and if leaf location within the head (lower or mid leaves) differs in nutritional composition. The experiment was conducted in the spring of 2023 in a high tunnel, and setup as a completely randomized design with 4 replications. At horticultural maturity (40 days after germination), the lower and mid leaves were harvested. Lettuce samples were frozen and shipped to Auburn University where samples were assayed for total chlorophyll, carotenoids, anthocyanins, flavonoids, phenolics and antioxidant activity via UV VIS spectrophotometry. Carotenoids of violaxanthin, neoxanthin, lutein and -carotene were quantitated by UPLC/MS. The interaction of leaf location*cultivar (P < 0.05) indicated that the lower leaves of ‘Skyphos’ lettuce had the highest total anthocyanin content at 2.94 mg/L compared to ‘Nancy’ at 0.05 mg/L, respectively. No other differences (P > 0.05) were detected between the cultivars. Sample location differed in total carotenoids (mg/g) and total chlorophyll (mg/g) with the lower leaves having the highest nutritional composition at 1380.18 and 5973.58 mg/g, respectively compared to the mid leaves (509.02 and 1684.42 mg/g, respectively). Chlorophyll a (4399.67 mg/g) and b (1573.90 mg/g) was also highest in the lower leaves of both cultivars compared to the mid leaves. Our results indicate that both butterhead lettuce cultivar and leaf location strongly impacts human health. ‘Skyphos’, a red butterhead lettuce, had the high highest total anthocyanin content in the lower leaves. Anthocyanins are critical phytochemicals known for many health promoting properties such as free radical scavenging, and anti-cancer and anti-diabetic properties. This experiment also indicated the lower leaves of both cultivars had the highest amount of total chlorophyll and carotenoids. The lower leaves of red butterhead lettuces, such as ‘Skyphos’, should be eaten to obtain the highest nutrition for their diet.
Speakers
MT

Marlee Trandel Hayse

Auburn University
Co-authors
AW

Alan Walters

Southern Illinois University
Wednesday September 25, 2024 11:00am - 11:15am HST
Nautilus 1

11:15am HST

PH 2 - Production System Alters Phytonutrient Content of ‘Skyphos’ Butterhead Lettuce
Wednesday September 25, 2024 11:15am - 11:30am HST
Lettuce is one of the most widely consumed vegetables in the world and can provide various health benefits to consumers. The type of production system, such as high tunnel, green roof or open field environments, can influence the nutritional composition of lettuce. To determine the extent of phytonutrient content change, ‘Skyphos’ lettuce was grown in these various production environments to compare phytonutrient content at harvest. The production experiment was conducted at Southern Illinois University-Carbondale and set up with three treatment locations and four replications. Two production systems used organic fertility practices (high tunnel and green roof), while the field production system used conventional fertilizers. At horticultural maturity (40 days after germination), the lower and mid leaves were harvested to determine phytonutrient content. Lettuce samples were frozen and shipped to Auburn University where samples were assayed for total chlorophyll, carotenoids, anthocyanins, flavonoids, phenolics and antioxidant activity via UV VIS spectrophotometry. Carotenoids of violaxanthin, neoxanthin, lutein and b-carotene were quantitated by UPLC/MS. The interaction of production system*leaf sampling location differed (P < 0.05) in total anthocyanins, total carotenoids and total chlorophyll. Total anthocyanins were highest in the lower leaves of ‘Skyphos’ lettuce cultivated on the green roof at 3.57 mg/L. In comparison, total carotenoids, total chlorophyll and chlorophyll a were highest in the lower leaves of lettuce cultivated in the high tunnel at 1316.48, 6093.45 and 4401. 97 mg/g, respectively. Cultivating lettuce in the high tunnel provided the highest Chlorophyll b content (1638.37 mg/g) compared to the field (888.80 mg/g) or green roof (893.43 mg/g). The phytonutrient content of lettuce can be influenced by location, temperature, soil type, humidity and UV radiation. The green roof increased total anthocyanin content which may be due to the higher temperatures and irradiation levels on compared to the field or high tunnel. Total Chlorophyll and carotenoid content was increased in the high tunnel likely due to increases of UV-A and UV-B exposure from the poly film leading to an increase in photosystem response. This experiment suggests the specific phytonutrient content (e.g., anthocyanin or carotenoids) in a red lettuce like ‘Skyphos’ differs based on production system and can be used in market development to increase consumer consumption by highlighting the specific phytonutrients highest in each production system.
Speakers
MT

Marlee Trandel Hayse

Auburn University
Co-authors
AW

Alan Walters

Southern Illinois University
Wednesday September 25, 2024 11:15am - 11:30am HST
Nautilus 1

11:30am HST

PH 2 - Improving Postharvest Storage and Late-Season Production of Caladium Tubers in Florida Through Chemical Treatments
Wednesday September 25, 2024 11:30am - 11:45am HST
Florida is home to the largest caladium production in the world, supplying essentially all the global caladium tuber demand. These plants are famous for their vibrant leaf colors and patterns and are asexually propagated through tubers. Following plant development, tubers are harvested and stored for a few months before being forced from March to September for potted plant production and direct landscape planting. During extended postharvest storage, caladium tubers risk severe weight loss, tissue decay, and Fusarium tuber rot. The current storage practices under ambient conditions with high temperatures and high relative humidity exacerbate weight loss and tuber rot. The challenges of long-term tuber storage make it difficult to produce pot caladium plants for winter holidays, including Thanksgiving, Christmas, and New Year. These challenges also affect the commercialization of tubers in the Southern Hemisphere during the summer. Opening these marketing opportunities can allow growers to extend and increase their production. Additionally, identifying caladium tubers suitable for late-season production will give Florida growers a competitive advantage. To identify caladium varieties with long-term tuber storage potential and late-season production, 12 varieties were evaluated to target Thanksgiving and Valentine’s Day. No.1-sized tubers were potted in 5-inch containers and grown in a greenhouse. For each variety, 10 tubers were monitored for sprouting and leaf expansion and later evaluated for plant quality using a rating scale from 1 to 5. Potted tubers from all cultivars achieved suitable market quality standards when targeting Thanksgiving, with the higher ratings obtained by cultivars ‘Splash of Wine’, ‘Classic Pink’, and ‘Lemon Blush’. The higher number of leaves and height were obtained by cultivars ‘Classic Pink’, ‘Desert Sunset’, and ‘White Wonder’, and ‘Party Punch’, ‘Ballet Slipper’, and ‘Classic Pink’, respectively. For Valentine’s Day, despite sprouting decreasing, cultivars ‘Splash of Wine’, ‘Hot 2 Trot’, ‘Desert Sunset’, ‘Party Punch’, ‘Classic Pink’, ‘White Wonder’, and ‘Lemon Blush’ reached suitable market quality standards. Overall, the number of leaves and height decreased compared to plants targeting Thanksgiving, however, cultivars with a more desirable number of leaves were ‘Classic Pink’ and ‘Lemon Blush’ whereas cultivars with higher height were ‘Party Punch’ and ‘Ballet Slipper’. Identifying cultivars suitable for late-season production will allow growers to expand their commercialization window for major holidays and to supply tuber demand to the Southern Hemisphere.
Speakers
GC

Gasselle Cordova

University of Florida
Co-authors
ZD

Zhanao Deng

University of Florida
Wednesday September 25, 2024 11:30am - 11:45am HST
Nautilus 1

11:45am HST

PH 2 - Impact of Storage Environment on Dormancy Progression and Microbiome of Potato Tubers
Wednesday September 25, 2024 11:45am - 12:00pm HST
Preservation of nutritional and market qualities of potato tubers during postharvest storage is essential for optimum economic return. Protecting tubers from unintended sprouting during storage is one of the major postharvest challenges that potato growers and stakeholders encounter. Storage environment, particularly storage temperature, significantly impacts dormancy progression and sprouting and effect overall marketability. Potato tubers carry significant amount of microbiome in different tissues of tubers from field to storage, which might contribute to overall storage qualities. However, how storage temperature impacts overall potato tuber microbiome and how changes in microbiome influence tuber dormancy are largely unknown. Therefore, the primary objective of this study was to investigate the impact of different storage temperatures on tuber microbiome and monitor the dormancy progression and sprout growth during long-term storage. Certified seed tubers of Russet Burbank were cured after harvest following industry practices. Then, one subset of tubers was stored at constant 8°C, and a second subset was stored at constant 21°C. Three types of tuber tissues (primary meristem, secondary meristem, and tuber flesh) were collected after 4, 8, 12, 17, and 24 weeks of storage for microbiome profiling and sugar and protein analysis. Additionally, a third subset was generated by moving tubers from 8°C to 21°C three days before sampling time of 8, 12, and 17 weeks. Tuber dormancy progression was monitored using additional subsets for each storage condition. As expected, the subset of tubers stored at 8°C had delayed sprouting compare to 21°C, and rapid sprout growth was observed when tubers were moved from 8°C to 21°C. Increase in reducing sugar content was observed in meristem tissues, while it decreased in tuber flesh with progression of storage duration. Interestingly, higher protein content was determined in secondary meristem tissues compare to primary meristems and tuber flesh. Overall, greater bacterial and fungal diversity and abundance were observed in meristem tissues when compared to tuber flesh. Additionally, storage temperatures, storage time, and tuber tissue types significantly impacted tuber microbial profile. In tuber tissues, Ascomycota and Basidiomycota were predominant fungal phyla, while Actinobacteria, Proteobacteria, and Cyanobacteria were the predominant bacterial phyla. Our results suggested that storage temperature and storage duration significantly impact both dormancy progression and tuber microbiome and subsequently contribute to postharvest qualities of potato tubers.
Speakers
MD

Munevver Dogramaci

United States Department of Agriculture
Co-authors
DS

Dipayan Sarkar

United States Department of Agriculture
NA
EF

Evandro Fortini

United States Department of Agriculture
NA
MB

Malick Bill

United States Department of Agriculture
NA
SK

Shyam Kandel

United States Department of Agriculture
NA
Wednesday September 25, 2024 11:45am - 12:00pm HST
Nautilus 1

12:00pm HST

PH 2 - Strawberry Anthocyanins, Extraction, Assessments and Storage
Wednesday September 25, 2024 12:00pm - 12:15pm HST
The anthocyanin pigments have antioxidant activities and play critical roles in plant and human health. They are abundant in flowers, vegetables, and fruits and are soluble in water, methanol, and nonpolar solvents such as chloroform. Extracting stable pigments with higher concentrations has been the research community's and industries' goal. Spectrophotometric methods can easily measure the total anthocyanin content. Other complex and more expensive methods, such as high-pressure liquid chromatography and ultrasound-assisted methods, are also available to identify and quantify the anthocyanin pigment. Strawberry color affects the appearance, fruit quality after harvest, and consumers' decision-making process. A large group of factors, such as sample type, temperature, pH, solvent type, and the ratio of its components, affect strawberry anthocyanin yield. We studied the effect of some of these variables on the anthocyanin yield, profile, and color of strawberries. Extraction solvents significantly changed the anthocyanin yield. The results suggest that acidified chloroform-methanol extracted the highest anthocyanin content compared to water-based solvents. Methanol-water-based solvents also performed better than water alone. Processing time (incubation time) was lowest in the pH differential method; however, the haze produced in this method may interfere with the spectrophotometry. Chloroform-methanol solvent with higher pH extracted pelargonidin as the main anthocyanin, and methanol and water-based solvents extracted delphinidin in UHPLC. Chloroform extracts reduced the redness and increased the extracts' brightness mainly due to higher pelargonidin content. Less redness and increased brightness indicated some level of color degradation of the extracts after storage for 48h at 4 °C.
Speakers
Wednesday September 25, 2024 12:00pm - 12:15pm HST
Nautilus 1

12:15pm HST

PH 2 - Isolation of Pathogenic Strain of Trichoderma atroviride from Symptomless, Disease-Free Sweetpotato (Ipomoea batatas L.) Storage Roots.
Wednesday September 25, 2024 12:15pm - 12:30pm HST
Many fungal endophytes have the ability to promote plant growth, as well as increase the host plant’s tolerance to abiotic and biotic stresses. Most endophytic species of Trichoderma are well known biocontrol agents and have been used to control diseases caused by phytopathogens of the genera Rhizoctonia, Fusarium, and Phytophthora. However, our recent research has isolated a strain of Trichoderma from sweetpotato storage roots purchased from a local farm, which was shown to develop root rot symptoms. Therefore, the objective of this project was to evaluate this isolate, especially for its potentially pathogenic nature. In the laboratory, the ITS region of the isolate was amplified, and gene sequencing placed it to T. atroviride with 99% homology. However, T. atroviride has never been identified as a disease-causing agent for sweetpotato in literature so far. Pathogenicity test was carried out accordingly, by inoculating this isolate onto healthy, symptomless sweetpotato storage roots. Two weeks post-inoculation, the storage roots were cut in half to reveal necrotic lesion development between 1.7 and 2.1cm in diameter based on three replications. The necrotic tissue was sampled and cultured on PDA, and the re-isolated fungal specimen was confirmed to be T. atroviride through gene sequencing. This result demonstrated that under environmental conditions conducive for pathogen development, this strain of T. atroviride is capable of causing root rot disease in sweetpotatoes. This finding is particularly significant as farmers typically store sweetpotatoes for an extended time, sometimes up to a year, which increases the possibilities for disease occurrence. Dual-culture assays are still ongoing to determine if this strain of T. atroviride could be a beneficial endophyte under different storage conditions, especially when other fungal endophytes are co-existing in the same storage root.
Speakers
RN

Richard Noel Torres

University of Missouri - Columbia
Co-authors
JS

James Schoelz

University of Missouri - Columbia
NA
PT

Peng Tian

University of Missouri - Columbia
NA
XX

Xi Xiong

University of Missouri - Columbia
NA
Wednesday September 25, 2024 12:15pm - 12:30pm HST
Nautilus 1

2:00pm HST

Fruit, Vegetable, and Edible Crops Collaboration Session
Wednesday September 25, 2024 2:00pm - 3:00pm HST
A forum for discussion of potential collaborations with regards to fruit, vegetable, and edible crops – i.e. citrus, breeding, production systems, postharvest, pomology, crop management, viticulture, etc.
Wednesday September 25, 2024 2:00pm - 3:00pm HST
Coral 4
 
Thursday, September 26
 

7:59am HST

Postharvest 3 (PH 3)
Thursday September 26, 2024 7:59am - 10:15am HST
Assessing Apple Cultivar Responses to Low Oxygen Limit Determination Using Chlorophyll Fluorescence and Respiratory Quotient - Hnin Phyu Lwin
Understanding how physiological maturity and storage regime affect skin greasiness on WA 38 apples - Carolina Torres
Chlorophyll to Carotenoid Ratio Measured Nondestructively Correlates with Peel Pigment Content and Canopy Attachment Position of ‘Granny Smith’ Apples - Manoella Mendoza
Early Spectral Differences on Scald Disorders in ‘Granny Smith’ Apples - Rene Mongollon
Apple Ripening After DCA Storage: Assessing The Risk Of Flavor Loss - Randolph Beaudry
The Impact Of Hot Water Treatment On Organic Apple Quality With Different Storage Systems - Sadat Amankona
Fungicides and Scald Inhibitor Diphenylamine Shift Microbiomes of ‘Fuji’ Apples Through Short Term Cold and Room Temperature Storage - Connor Lane
Pyrus communis: A Model for Studying Climacteric Ripening and Senescence - Amit Dhingra
Characterization of Postharvest Rots and the Susceptibility to Decay of 38 European Pear Genotypes during Cold Storage. - Tamara Collum
Moderator
avatar for Manoella Mendoza

Manoella Mendoza

WA State University
Thursday September 26, 2024 7:59am - 10:15am HST
Nautilus 1

8:00am HST

PH 3 - Assessing Apple Cultivar Responses to Low Oxygen Limit Determination Using Chlorophyll Fluorescence and Respiratory Quotient
Thursday September 26, 2024 8:00am - 8:15am HST
Dynamic controlled atmosphere (DCA) is a prominent technology used worldwide for long-term apple storage. In Washington, the adoption of DCA technologies to manage low oxygen levels is limited, partly because the benefits are not clearly demonstrated for some major cultivars, such as ‘Honeycrisp’ and new varieties like ‘WA 38’. The response of ‘Honeycrisp’, ‘Gala’, and ‘WA 38’ apples to lower oxygen levels was monitored monthly until 8 months of storage (1oC or 3oC) through chlorophyll fluorescence (CF) and respiratory quotient (RQ) by a stepwise decrease in O2 levels at each time point during 2023/2024 season. Ethanol and acetaldehyde concentrations were obtained from fruit juice before and after the lower oxygen limit (LOL) determinations. ‘Honeycrisp’ and ‘Gala’ apple cultivars showed differences in LOLs determined through CF or RQ. CF LOL for ‘Honeycrisp’ was approximately 0.3-0.2 kPa O2 compared to 0.1 kPa O2 for ‘Gala’ while RQ response occurred at 0.1 kPa O2 level in both cultivars. ‘WA 38’ apples displayed inconsistent CF responses at 0.3 and 0.4 kPa oxygen levels. Ethanol concentration increased after low oxygen stress irrespective of the cultivar. During the first month of storage and after the low oxygen stress, ‘Honeycrisp’ apples accumulated (in average) 120 ppm of ethanol, ‘Gala’ 2.92 ppm, and ‘WA 38’ 4.24 ppm. Towards the end of storage, the ethanol concentration showed an increase for ‘Gala’ (25 ppm), which was not observed for the other two cultivars. Small amounts of acetaldehyde were accumulated only in ‘Honeycrisp’ apples. This study highlights the different responses of apple cultivars to low oxygen stress during the lower oxygen limit (LOL) determination, with ‘Honeycrisp’ being the most sensitive one among the cultivars tested. Further seasons are needed to confirm these results and include the seasonal-dependent responses in each cultivar.
Speakers
HP

Hnin Phyu Lwin

Washington State University
Co-authors
CT

Carolina Torres

Washington State University
DR

David Rudell

USDA-ARS
NA
SA

Sadat Amankona

Washington State University-TFREC
I am a second-year Ph.D student at Washington State University-TFREC
Thursday September 26, 2024 8:00am - 8:15am HST
Nautilus 1

8:15am HST

PH 3 - Understanding how physiological maturity and storage regime affect skin greasiness on WA 38 apples
Thursday September 26, 2024 8:15am - 8:30am HST
‘WA 38’ (Cosmic Crisp®) is the newest cultivar released by the Washington State University apple breeding program, and today in its sixth year of commercial production in WA. This cultivar has a high pack-out, i.e. highly colored fruit and few external and internal defects. Nevertheless, skin greasiness has been one of the main defects at harvest and postharvest, making waxing during packaging challenging, and affecting the cosmetic appearance. Since the starch degradation index is the only maturity index used to commercially harvest WA 38, the correlation between maturation and the onset of greasiness was studied during two seasons. Different maturity indices pre-and postharvest (firmness, soluble solid content, internal ethylene concentration (IEC), starch index (1-6), IAD values) along with skin greasiness were evaluated from 3-4 weeks before harvest (WBH) until 6 months in air (1oC) or controlled atmosphere (2.5 kPa O2, 1.5 kPa CO2) storage in fruit from four commercial orchards (#1 to #4; 2nd-4th-leaf). In 2022 and 2023 the rate of change of all maturity indices were block-dependent. In both years, IEC was detected in up to 80% of the fruit sampled four WBH in orchard #1 (average 0.3 ppm). At this same time point, only 16% of the fruit had started the starch degradation process (SI=1.5/6.0). The climacteric peak occurred 3-5 months into cold storage, and it was block-dependent. Greasiness on fruit skin was detected 2 WBH in two of the four orchards in 2022; in 2023 this occurred at commercial harvest. Overall, there was a higher incidence (and severity) of greasiness in fruit from air storage compared to CA, and in both cases, it increased during the shelf-life period (7 d at 20oC). The results indicate that fruit greasiness appears to be more related to fruit maturity and perhaps seasonal weather than tree age, nevertheless, additional seasons are needed to confirm these results.
Speakers
CT

Carolina Torres

Washington State University
Co-authors
DR

David Rudell

USDA-ARS
NA
OG

Oswaldo Gonzalez

Washington State University
NA
RM

Rene Mogollon

Washington State University
Thursday September 26, 2024 8:15am - 8:30am HST
Nautilus 1

8:30am HST

PH 3 - Chlorophyll to Carotenoid Ratio Measured Nondestructively Correlates with Peel Pigment Content and Canopy Attachment Position of ‘Granny Smith’ Apples
Thursday September 26, 2024 8:30am - 8:45am HST
‘Granny Smith’ is an important apple variety for Washington State, where it has consistently been ranked as the third most-produced conventional cultivar since 2017. This apple variety is highly susceptible to sunscald, a sun-stress-related postharvest disorder that typically appears after three months of cold storage and makes apples unmarketable. Previous research has shown that a decrease in chlorophyll (chl) and an increase in carotenoids (car) in fruit peel have been associated with increased sunlight exposure and the development of sunscald. In order to assess the use of the chl/car ratio measured non-destructively with a Vis/NIR hyperspectral imager as a predictive index for sun exposure and sunscald development postharvest, ‘Granny Smith’ apples were picked from the periphery and inner canopy sections of the tree in 2021 and 2023 seasons. Fruit position in the canopy was documented during sampling and later correlated with hyperspectral images, chlorophyll, and carotenoid content in the peel. Subsequently, the collected peel sample was immediately frozen with liquid nitrogen and stored at -80°C. The chl/car ratio obtained from the reflectance values from the hyperspectral images was well correlated (r2 = 0.82) with the destructive determination of the ratio. Based on k-means clustering analysis, samples were grouped in accordance with the chl/car ratio and associated with fruit position on the tree. Apples with the highest chl/car ratios (1.4 to 1.7) were those located on the shaded portion of the trees. Apples with sunburn had the lowest chl/car ratios (0.1 to 1.2) and were located on the periphery of the tree. The results indicate that the chlorophyll to carotenoid ratio measured non-destructively at harvest can potentially be used for pre-storage sorting of ‘Granny Smith’ apples based on relative sun exposure and sunscald susceptibility, reducing postharvest losses. However, the establishment of the index reliability can benefit from additional years of data collection.
Speakers
avatar for Manoella Mendoza

Manoella Mendoza

WA State University
Co-authors
CT

Carolina Torres

Washington State University
DR

David Rudell

USDA - ARS
NA
LK

Lee Kalcsits

Washington State University
NA
RM

Rene Mogollon

Washington State University
Thursday September 26, 2024 8:30am - 8:45am HST
Nautilus 1

8:45am HST

PH 3 - Early Spectral Differences on Scald Disorders in ‘Granny Smith’ Apples
Thursday September 26, 2024 8:45am - 9:00am HST
‘Granny Smith’ apples are highly susceptible to skin browning (=scald). These disorders can significantly reduce fruit’s marketability due to their unattractive appearance. Superficial scald and sunscald have different etiologies but their symptoms are similar and easily mistaken. The oxidation of α-farnesene causes superficial scald (SS); on the other hand, sunscald (SC) is a non-oxidative process that affects only the sunlit sides of the fruit. To correlate the spectral fingerprint with fruit susceptibility to these disorders, hyperspectral images (400-1000 nm, 640x865 px; Headwall Photonics, Bolton, MA) were taken at harvest from sun-exposed and unexposed sides of the fruit (n=216; ~26,000 px) and later, after six months in air storage (33 oF), correlated with scald incidence. The dataset grouped 145 asymptomatic, 117 with SS and 170 with SC fruit. After pre-processing spectral information (Savitzky-Golay dev, standard normal variate), iPLS wavelength selection showed that bands from 400 to 650 and 900 to 950 nm were the most accurate for at-harvest spectral differentiation between superficial and sunscald symptoms. A neural network classification model was trained (18,226 px) and validated on an independent dataset (7,808 px), achieving overall accuracies of 78 % and 73 %, respectively. After prediction, SC px obtained the highest classification metrics (accuracy 87 %, precision 86 %); meanwhile, the asymptomatic class showed the lowest metrics (accuracy 74 %, precision 60 %). These preliminary results showed that in the same way, sunscald could be identified close to harvest using hyperspectral fingerprints, superficial scald could also be predicted at harvest on susceptible fruit and differentiated from sunscald susceptible fruit.
Speakers
RM

Rene Mogollon

Washington State University
Co-authors
CT

Carolina Torres

Washington State University
DR

David Rudell

USDA-RS
NA
MM

Manoella Mendoza

WA State University
Thursday September 26, 2024 8:45am - 9:00am HST
Nautilus 1

9:00am HST

PH 3 - Apple Ripening After DCA Storage: Assessing The Risk Of Flavor Loss
Thursday September 26, 2024 9:00am - 9:15am HST
Dynamic controlled atmosphere (DCA) storage suppresses the ripening of apple fruit during storage to a greater extent than standard CA storage. DCA is a relatively new technology, and it has the potential to markedly alter the volatile profile of apple fruit. It has been found that DCA storage can suppress the most important aroma volatiles of stored apple fruit. What we don't know is the length of time and the conditions needed to recover that loss in aroma, especially when DCA is applied in combination with the ethylene action inhibitor 1-methylcyclopropene (1-MCP). A critical concern about 1-MCP is that apple flavor is compromised in treated fruit because aroma volatiles are so closely linked with ethylene response. Apple fruit from high-value cultivars were stored under a DCA regimen (approximately 0.4% O2), with and without 1-MCP treatment, for 3, 6, and 9 months. Following removal of fruit from DCA, the volatile profile and fruit quality traits were measured for up to 5 weeks while being held at room temperature. The recovery of aroma formation following storage for 6 months was essentially immediate for ‘Red Delicious’ fruit if 1-MCP was not used. The recovery was a little slower for one of the replicate studies if DCA was used. If the fruit were treated with 1-MCP, there was a 2-week delay in the recovery of aroma formation. ‘Evercrisp’ aroma formation was much lower than ‘Red Delicious’ and the responses to DCA and 1-MCP differed from that of Red Delicious. Aroma formation remained very low for 3 to 4 weeks at room temperature following CA and DCA storage and never recovered following 1-MCP treatment for the 5-week duration of the study. The data for aroma formation after 9 months was similar to that at the 6-month time point for ‘Red Delicious’ in terms of 1-MCP responses. However, the effect of DCA seemed to be more pronounced, suppressing aroma recovery for an additional week at room temperature. For ‘Evercrisp’, the 9-month data were quite similar to those from 6 months storage.
Speakers
RB

Randolph Beaudry

Michigan State University
Dr. Randolph Beaudry, Professor, MSU Department of Horticulture (MSc, PhD, University of Georgia). His appointment is 50% MSU Extension and 50% AgBioResearch. His research program includes both regional and international projects and focuses on the physiology of plant responses to... Read More →
Co-authors
JX

Jiarui Xu

Michigan State University
NA
NS

Nobuko Sugimoto

Michigan State University
NA
OH

Ozge Horzum

Ankara University
NA
Thursday September 26, 2024 9:00am - 9:15am HST
Nautilus 1

9:15am HST

PH 3 - The Impact Of Hot Water Treatment On Organic Apple Quality With Different Storage Systems
Thursday September 26, 2024 9:15am - 9:30am HST
There has been a surge in demand for organic apples in the U.S. in the last decade. Washington State supplies more than 90% of them because of the ideal climate and soil conditions that favor production. Thermotherapy methods are widely used in Europe to reduce postharvest rots, but they have not been adopted in the U.S. because of their potential detrimental effects on fruit quality. The objective of this research was to evaluate the impact of hot water treatments at harvest on fruit quality during long-term storage. ‘Royal Gala’ and ‘Honeycrisp’ apples were immersed at 49 °C or 52 °C for 2 minutes in a controlled hot water bath immediately after harvest. After fruits were dried at 20 °C, they were placed in controlled atmosphere (CA; 2 kPa O2, 0.5 kPa CO2, 1°C-‘Royal Gala’, 3oC-‘Honeycrisp’) and air (RA; 1°C-‘Royal Gala’, 3oC-‘Honeycrisp’) storage for up to 9 months. Fruit quality (firmness and titratable acidity), defects, and physiological disorders were evaluated 1 and 7 days at 20 °C. In general, ‘Royal Gala’ apples immersed at 49 °C and stored in CA had no decay after 3 and 6 months of storage, but it was the highest (around 9%) in ‘Honeycrisp’ in both CA and RA. Peel damage was less at 49 °C for both ‘Royal Gala’ (RA-1.3%; CA-5%) and ‘Honeycrisp’ (RA-7.4%; CA-2.8%) compared to 52 °C after 3 months. Fruit firmness and titratable acidity were statistically different (p≤0.05) at 49 °C compared to 52 °C after 3 months. Although the hot water treatment in ‘Royal Gala’ apples was effective in reducing decay incidence without compromising fruit quality, the opposite was found in ‘Honeycrisp’; therefore, further research is needed to confirm these results and understand the variety-dependent effect.
Speakers
avatar for Sadat Amankona

Sadat Amankona

Washington State University-TFREC
I am a second-year Ph.D student at Washington State University (Tree Fruit Research & Extension Center). My research is about using non-chemical solutions for long-term storage of organic apples.
Co-authors
CT

Carolina Torres

Washington State University
Thursday September 26, 2024 9:15am - 9:30am HST
Nautilus 1

9:30am HST

PH 3 - Fungicides and Scald Inhibitor Diphenylamine Shift Microbiomes of ‘Fuji’ Apples Through Short Term Cold and Room Temperature Storage
Thursday September 26, 2024 9:30am - 9:45am HST
Recent studies have shown that the native microbiome plays an important role in biocontrol efficacy, but in comparison less is known about how the microbiome responds to conventional and natural product fungicides. This study investigated the effects of conventional fungicide fludioxonil, natural product fungicide natamycin, and scald inhibitor diphenylamine (DPA) on the microbiomes of ‘Fuji’ apples (Malus x domestica) from 1-28 days of cold storage and 7 days of room temperature after the cold storage. We hypothesized that fludioxonil would have a smaller effect on the microbiome than natamycin due to being more limited in targeting specific pathogens, and that antioxidant activity from DPA would shift the microbiome. DPA showed a strong effect on the fungal microbiome that increased with storage time, while natamycin resulted in modest fungal shifts and fludioxonil resulted in no observed shifts. While chemical treatment was not a predictor of bacterial microbiome variation, bacterial communities shifted throughout storage with many of the trends reversing when samples were removed and left at room temperature. Time in cold storage decreased the relative abundance of Pseudomonas and DPA application reduced the relative abundance of Aureobasidium, both notable biocontrol genera. These results highlight how chemical applications like DPA may have unintended effects on beneficial microbes that protect crops from pathogen infection.
Speakers
CL

Connor Lane

Cornell University
Co-authors
CW

Chris Watkins

Cornell University
NA
JK

Jenny Kao Kniffin

Cornell University
NA
YA

Yosef Al Shoffe

Cornell Univeristy
Thursday September 26, 2024 9:30am - 9:45am HST
Nautilus 1

9:45am HST

PH 3 - Pyrus communis: A Model for Studying Climacteric Ripening and Senescence
Thursday September 26, 2024 9:45am - 10:00am HST
Fruits constitute a critical part of a healthy diet. However, they often perish prior to consumption due to unpredictable ripening. Especially in climacteric fruit, blockage in the perception or synthesis of ethylene has been used to prolong shelf life. European pear (Pyrus communis) are classified as climacteric fruit. However, System 2 ethylene production requires a genetically pre-determined period of cold conditioning, which triggers ripening—in addition, applying 1-MCP, an ethylene perception inhibitor, indefinitely blocks ripening. Using a physiological and developmental transcriptomics approach, we have shown that in 1-MCP fruit stored in a controlled atmosphere, activating alternative respiration via glyoxylic acid stimulation of AOX expression can reverse the effect of 1-MCP. Interestingly, 1-MCP fruit without CA storage responds differently to glyoxylic acid. It shows classic signs of senescence. The underlying processes of ripening and senescence can be discerned by utilizing the fruit that has been treated differently post-1-MCP application. This knowledge is expected to aid in identifying strategies to prolong fruit shelf life.
Speakers
avatar for Amit Dhingra

Amit Dhingra

Head of the Department of Horticultural Sciences, Texas A&M University
Dr. Amit Dhingra is the Head of the Department of Horticultural Sciences, Professor of Genomics and Biotechnology, and Senior Scientist at the Norman Borlaug Institute for International Agriculture at Texas A&M University. After his post-doctoral training at Rutgers University, the... Read More →
Co-authors
EC

Evan C. Stowe

Washington State University
NA
SL

Seanna L. Hewitt

Washington State University
NA
Thursday September 26, 2024 9:45am - 10:00am HST
Nautilus 1

10:00am HST

PH 3 - Characterization of Postharvest Rots and the Susceptibility to Decay of 38 European Pear Genotypes during Cold Storage.
Thursday September 26, 2024 10:00am - 10:15am HST
Phytopathogenic fungi can cause rots during cold storage leading to the loss of pear fruit and significant economic costs. Currently, all commercially available pear varieties are susceptible to postharvest rots. Genetic resistance to fruit rot pathogens including Penicillium expansum and Colletotrichum spp. has been identified in wild apple germplasm. The objective of this study was to evaluate diverse genotypes of European pears (Pyrus communis L.) for disease incidence during cold storage and evaluate susceptibility to prevalent phytopathogenic fungi by directly challenging fruit using a wound inoculation method. Fruit from 38 pear genotypes were harvested at maturity, then evaluated weekly for the presence of postharvest rots during cold storage. If rot was observed, the diseased fruits were removed from cold storage and fungal isolates were collected from fruit with disease symptoms. The identity of fungal isolates was determined based on morphology and genetic sequencing of marker genes including ITS and TEF1. After 12 weeks in cold storage, the incidence of rot ranged from 8.3% to 100%. Colletotrichum was the most prevalent genus isolated from the rotting pears. Twenty pear genotypes were directly challenged with P. expansum or C. fioriniae using a wound inoculation method on fruit that was harvested at commercial maturity. Five genotypes had significantly reduced disease development when challenged with P. expansum compared to susceptible varieties Gem and Bartlett. Four of these five genotypes also had significantly reduced disease development when challenged with C. fioriniae. One additional genotype was found to have significantly reduced disease development when challenged with C. fioriniae but was highly susceptible to P. expansum. These results will be a valuable resource for pear breeders and aid efforts to develop European pear varieties that have genetic resistance to fruit rot pathogens.
Speakers Co-authors
BE

Breyn Evans

USDA ARS
NA
CB

Caitlin Barnes

USDA ARS Appalachian Fruit Research Station
NA
CG

Christopher Gottschalk

USDA ARS
During his Ph.D. studies, Dr. Gottschalk studied the molecular mechanisms that control flowering in apple as they relate to seasonal bloom times and biennial/alternate bearing in diverse apple germplasm, from wild species to cultivated varieties. Moreover, he investigated plant growth... Read More →
JB

John Bennett

USDA ARS
NA
Thursday September 26, 2024 10:00am - 10:15am HST
Nautilus 1

12:14pm HST

Postharvest 2 (PH 2)
Thursday September 26, 2024 12:14pm - 2:00pm HST
Non-invasive Assessment of Haskap Growth (Lonicera caerulea) Utilizing X-ray Computed Tomography and Image Processing - Arturo Duarte Sierra
Vase Life Prediction for Cut Roses Using Deep Learning and Hyperspectral Imagining Techniques - Yongtae Kim
Extending the Postharvest Storage of Caladium Tubers to Unlock Late-Season Pot Plant Production Potential in Florida - Gasselle Cordova
Analysis of Oxidative Decreases in Hop (Humulus lupulus L.) Bitter Acids Produced Postharvest - Katie Stenmark
The Use of Desiccants for Proper Moisture Preservation in Green Coffee During Storage and Transportation - Laudia Anokye Bempah
Transcriptome Analysis Revealed that Metabolites and Hormone Signaling Pathway Differently Respond to Non-chilling and Chilling Temperatures in Cucumber (Cucumis sativus L.) Fruit During Storage - Yujin Sim
Fresh Weight of Indoor-Grown Lettuce under Different Postharvest Storage Practices -Caroline Blanchard
Modified Hydrocooling and Optimized Postharvest Handling Practices Can Improve the Shelf Life of Water Spinach During Summer in Humid Subtropical Regions - Wei Shiang Liao
Discovery of Biomarkers for Postharvest Pecan Color Stability Using Metabolomics and Machine Learning Algorithms - Min Jeong Kang
Evaluating the Effects of Controlled Atmosphere Storage on Two Georgia-grown Pecan Cultivars - Ramsey Corn

Moderator
DA

Duarte-Sierra Arturo

Université Laval
Thursday September 26, 2024 12:14pm - 2:00pm HST
South Pacific 3

12:15pm HST

PH 2 - Non-invasive Assessment of Haskap Growth (Lonicera caerulea) Utilizing X-ray Computed Tomography and Image Processing
Thursday September 26, 2024 12:15pm - 12:25pm HST
The latest advances in technology have made it easier to accurately assess the quality of fresh produce, reducing the amount of physical handling required. Computed tomography (CT) is a non-intrusive method that can effectively monitor and categorize fresh produce at different stages of development and maturity. Implementing novel CT scanning techniques can provide innovative means for classifying fresh produce in the early stages of development. The present work provides information for the calculation of the volume and the porosity of the haskap during its ontology. Samples of haskap were taken at four stages of growth prior to harvest and at the time of harvest. Image data were acquired using X-ray microcomputed tomography (SkyScan 1272, Bruker, Billerica, MA, USA) at a resolution of 19.69 μm per pixel, operating at 60 kV and 166 μA, using a 0.25 mm aluminum filter. The resultant data was exported to the BMP file format. BMP files were then processed using Avizo 3.0 (Thermo Fisher Scientific, Waltham, Massachusetts, USA). Automated thresholding using factorization in criteria was applied to the 8-bit images. Subsequently, the images were labeled and an axis connectivity of 26 neighborhoods was established. Finally, a measurement was derived using the volume fraction tool. The results showed that the porosity at stage 3 was 26.56%. It then decreased to 7.52% in the 4th stage and reached a peak of 13.45% during the harvesting stage. These results may have implications for commercial cultivation strategies and optimization of pre- and post-harvest practices and add to the existing understanding of Haskap growth and development.
Speakers
DA

Duarte-Sierra Arturo

Université Laval
Co-authors
CG

Charles Goulet

Université Laval
NA
EA

Ernesto Alonso Lagarda Clark

Université Laval
NA
Thursday September 26, 2024 12:15pm - 12:25pm HST
South Pacific 3

12:25pm HST

PH 2 - Vase Life Prediction for Cut Roses Using Deep Learning and Hyperspectral Imaging Techniques
Thursday September 26, 2024 12:25pm - 12:35pm HST
Japan is a major export market for the Korean cut rose flower industry. The longevity of cut roses significantly influences consumer purchasing decisions, prompting Japan to establish a quality guarantee system to ensure cut flower longevity. However, existing longevity guarantee methods rely heavily on subjective evaluations, overlooking critical factors such as senescence characteristics and disease infections. Hyperspectral imaging (HSI) technique is used for quality control of many fruits and can be performed at video rates, and could thus provide accurate data on aspects of cut flower quality. The You Only Look Once version 8 (YOLOv8) and Random Forest models for object detection and classification enable consistent quality assessment and swift longevity prediction. In this study, we developed a non-contact and rapid detection technique for the potential longevity of cut roses using deep learning techniques based on HSI data. Cut ‘Unforgettable’, ‘Egg Tart’, and ‘Catalina’ rose flowers were held in wet conditions during the exportation to Japan. HSI data within the visible near-infrared range 450-900 nm wavelengths were obtained for analysis of the disease infection and quality of cut roses. Image data of diseased cut roses were collected and corresponding data processing was carried out to build diseased cut roses and quality detection dataset. We developed the longevity prediction model based on scoring a grading standard on the flower quality and this model was then used to predict the longevity and evaluate quality changes of cut roses after exporting to Japan. The results showed that the longevity of exported cut roses was 8 d (‘Egg Tart’), 5.9 d (‘Catalina’), and 4.9 d (‘Unforgettable’). The longevity of cut roses was primarily terminated by gray mold disease (‘Unforgettable’ and ‘Catalina’), petal wilting and discoloration (‘Egg Tart’ and ‘Catalina’), and petal abscission (‘Catalina’). The predictive accuracy of the three rose flowers longevity prediction model was three rose flowers ‘Egg Tart’ (r2=0.80), ‘Unforgettable’ (r2=0.78), and ‘Catalina’ (r2=0.65). These results demonstrate that the combination of HSI and deep learning is a reliable method for evaluating the longevity of exported cut roses.
Speakers
YK

yongtae kim

Andong National University
Co-authors
BI

ByungChnu In

Andong National University
NA
JY

Ji Yeong Ham

Andong National University
ST

Suong Tuyet Yhi Ha

Andong National University
NA
Thursday September 26, 2024 12:25pm - 12:35pm HST
South Pacific 3

12:35pm HST

PH 2 - Extending the Postharvest Storage of Caladium Tubers to Unlock Late-Season Pot Plant Production Potential in Florida
Thursday September 26, 2024 12:35pm - 12:45pm HST
Florida is home to the largest caladium production in the world, supplying essentially all the global caladium tuber demand. These plants are famous for their vibrant leaf colors and patterns and are asexually propagated through tubers. Following plant development, tubers are harvested and stored for a few months before being forced from March to September for potted plant production and direct landscape planting. During extended postharvest storage, caladium tubers face the risk of severe weight loss, tissue decay, and Fusarium tuber rot. The current practice of storage under ambient conditions with high temperatures and high relative humidity exacerbates weight loss and tuber rot. The challenges of long-term tuber storage make it difficult to produce pot caladium plants for winter holidays, including Thanksgiving, Christmas, and New Year. These challenges also affect the commercialization of tubers in the Southern Hemisphere during the summer. Opening these marketing opportunities can allow growers to extend and increase their production. Additionally, the identification of caladium tubers suitable for late-season production will give Florida growers a competitive advantage. To identify caladium varieties with long-term tuber storage potential and late-season production, 12 varieties were evaluated to target Thanksgiving and Valentine’s Day. No.1-sized tubers were potted in 5-inch containers and grown in a greenhouse. For each variety, 10 tubers were monitored for sprouting and leaf expansion and later evaluated for plant quality using a rating scale from 1 to 5. Eight cultivars were identified as suitable to grow for Thanksgiving, whereas 3 were identified for Valentine’s Day based on long-term storage potential and plant performance. Identification of cultivars will allow growers to expand their commercialization window not only for major holidays but also to supply tuber demand to the Southern Hemisphere.
Speakers
GC

Gasselle Cordova

University of Florida
Co-authors
SS

Steven Sargent

University of Florida
Professor and Extension Postharvest Specialist
ZD

Zhanao Deng

University of Florida
Thursday September 26, 2024 12:35pm - 12:45pm HST
South Pacific 3

12:45pm HST

PH 2 - Analysis of Oxidative Decreases in Hop (Humulus lupulus L.) Bitter Acids Produced Postharvest
Thursday September 26, 2024 12:45pm - 12:55pm HST
Nearing maturity, female hop plants develop inflorescences called hop cones. Inside of a mature hop cone is the lupulin gland where glandular trichomes are present. Within the lupulin gland, the glandular trichomes secrete alpha acids (α), and beta acids (β). Hop bitter acids are extremely sensitive to photolysis and oxidation reactions causing the degradation of α-acids and β-acids which negatively affect their use in beer, and the beer brewing industry accounts for 98% of world use of hops (Fandino et al., 2015). Determining hop storage index (HSI) is a way to measure the amount of α and β-acids lost during postharvest handling. HSI is a ratio determined by measuring the spectrophotometric UV absorption of hop extract at 275nm for oxidative compounds, and 325nm for bitter acids. Bitter acids and any oxidative decreases were determined in eight cultivars of hops (‘Cascade’, ‘Chinook’, ‘Comet’, ‘Mount Rainier’, ‘Newport’, ‘Tahoma’, ‘Willamette’, ‘Zeus’) produced at the Cimarron Valley Research Station in Perkins, Oklahoma. Mature hop cones were hand harvested at 80% moisture and dried at ambient temperature to 8-10% moisture using a centrifugal fan. Hops were stored no longer than six months frozen under nitrogen in vacuum sealed bags until analysis. Hop bitter acids were extracted using toluene and UV absorbance was measured at 355, 325, and 275nm using a spectrophotometer. Based on the HSI, hops were assigned a quality score of good (0.4). Seven of the eight cultivars of hops were of good quality (‘Cascade’, 0.20; ‘Chinook’, 0.19; ‘Comet’, 0.17; ‘Mount Rainier’, 0.12; ‘Newport’, 0.23; ‘Tahoma’, 0.18; ‘Willamette’, 0.22). ‘Zeus’ hops were of questionable quality (0.31) but contained a slightly higher moisture content at storage (11%) than the other cultivars (7-10%), which could have negatively affected hop storage quality. Understanding sources of oxidative stresses to hop bitter acids during postharvest handling is valuable information for determining and maintaining hop quality.
Speakers
KS

katie stenmark

OKLAHOMA STATE UNIVERSITY
Crop Science PhD Student Department of Horticulture and Landscape Architecture Oklahoma State University
Co-authors
CF

Charles Fontanier

OKLAHOMA STATE UNIVERSITY
NA
NM

NIELS MANESS

OKLAHOMA STATE UNIVERSITY
NA
Thursday September 26, 2024 12:45pm - 12:55pm HST
South Pacific 3

12:55pm HST

PH 2 - The Use of Desiccants for Proper Moisture Preservation in Green Coffee During Storage and Transportation
Thursday September 26, 2024 12:55pm - 1:05pm HST
Prolonged storage and long-distance transportation of green coffee beans exposes them to undesirable fluctuations in temperature (T) and relative humidity (r.h.), which can change the physical (wet-basis moisture content (MCwb), water activity (Aw), and color) and sensory characteristics of the coffee. High humidity also supports mold growth, decay, and microbial activities. Thus, the objective of this study was to evaluate the efficacy of commercially available desiccants for preserving the moisture content of green coffee between 10 and 12% MCwb, when stored in either hermetic packages and/or jute sacks, and to assess the corresponding impact on sensory quality. A conventional coffee storage and transportation period from Brazil to Italy with a duration of 42 days was mimicked in environmental chambers. Treatments in a 3 × 3 factorial design consisting of three packaging materials (GrainPro SuperGrain bag, GrainPro TranSafeliner, and/or jute sacks) and desiccants (Drying Beads®, CaCl2, or no desiccants) were evaluated. Additionally, four different mass ratios of green coffee to desiccant ranging from 50 to 300 – g coffee per g desiccant were also evaluated. The MCwb, Aw, and color of all samples were measured approximately weekly over 42 days. In comparison to the control (no desiccant, and only jute sacks), we observed a statistically significant impact for all tested desiccants and hermetic packages for maintaining the proper MCwb, Aw, and color. No significant difference was observed for the different desiccant masses tested when they were placed inside the hermetic packaging, but the desiccants were ineffective without the hermetic packaging. Triangle test and descriptive sensory evaluation yielded no significant differences between the use of hermetic packages with or without desiccants.
Speakers
LA

Laudia Anokye Bempah

UC DAVIS
Laudia Anokye-Bempah is a Ph.D. student in the Biological Systems Engineering Program at the University of California Davis. Her research focuses on the kinetics of coffee roasting, with the ultimate goal of designing a “Coffee Roasting Control Chart” that will be used to produce... Read More →
Thursday September 26, 2024 12:55pm - 1:05pm HST
South Pacific 3

1:05pm HST

PH 2 - Transcriptome Analysis Revealed that Metabolites and Hormone Signaling Pathway Differently Respond to Non-chilling and C
Thursday September 26, 2024 1:05pm - 1:15pm HST
Cucumber (Cucumis sativus L.) fruit is sensitive to chilling temperatures during storage, and the physiological mechanism of chilling injury (CI) is not well known. Understanding the chilling response in cucumber fruit is necessary to delay CI and extend the storage duration during postharvest. In this study, our goal was to identify CI symptoms and investigate key factors influencing on the chilling response in cucumber fruit (C. sativus var. ‘Hangang’). To assess the severity of CI symptoms, cucumber fruits were stored at low temperatures of both 13 °C (non-chilling response) and 2 °C (chilling response) for 9 d and exposed to 20 °C. As a result, various CI symptoms of discoloration, shirankage, water-soaking and necrosis area appeared on a cucumber fruit peel stored at 2 °C, resulting in loss of its marketability from 6 d. To clear the cellular mechanism of CI symptoms, transcriptome analysis was conducted in cucumber fruit stored for 1 d (early response) and 6 d (late response). The results suggested that phytohormone synthesis and signalling played major roles in chilling responses. To reveal the main phytohormone involved in the chilling response, eight phytohormones were quantified in cucumber fruit peel using LC-MS/MS. Among them, ABA was not sufficiently accumulated at 2 °C compared to 13 °C during the early response, and SA levels gradually increased by persisting chilling stress. Through these results, major phytohormone synthesis and signalling genes were selected by RT-qPCR. For further understanding of CI, targeted metabolite analysis was conducted, and amino acids such as isoleucine, serine, valine, threonine, and sucrose were identified as significant metabolites for acclimating to chilling temperatures. These findings help to elucidate the hormonal mechanisms involved in chilling response as well as the complex interplay of various molecular components involved in chilling response of cucumber fruit during storage.
Speakers
YS

Yujin Sim

Seoul national university
Co-authors
EJ

Eun Jin Lee

Seoul National University
KM

Kyeonglim Min

Seoul National University
Thursday September 26, 2024 1:05pm - 1:15pm HST
South Pacific 3

1:15pm HST

PH 2 - Fresh Weight of Indoor-Grown Lettuce under Different Postharvest Storage Practices
Thursday September 26, 2024 1:15pm - 1:25pm HST
Two-thirds of food waste occurs once the product has reached the consumer. In an effort to extend the shelf-life of produce, a common practice of hydroponic lettuce growers, particularly those who use controlled environment production systems such as greenhouses or indoor growing facilities, is to harvest the lettuce without removing the root tissue. However, the postharvest quality of this “living lettuce” has not been a focus of academic research, and its effect on the shelf-life of the produce is unknown. In this study, lettuce harvested from an indoor production facility that utilizes a vertical hydroponic setup was subjected to four postharvest treatments. Treatments included harvesting the complete lettuce plant or removing the roots after harvesting and storing the harvested produce either inside or outside of a plastic clamshell. Lettuce was stored for 28 days at 4 °C and 85% relative humidity. Fresh weight of the lettuce heads was assessed for 18 days post-harvest. As days in storage increased, the fresh weight of lettuce decreased by 0.37 grams per day. The rate of lettuce fresh weight decrease was the same across postharvest treatments, but the lettuce with roots intact stored inside a plastic clamshell had a higher fresh weight across all measured time points. A better understanding of the relationship between fresh lettuce weight and storage time under different postharvest conditions can help to increase the shelf life of the stored produce and reduce food waste.
Speakers
CB

Caroline Blanchard

Research Associate II, Auburn University
Co-authors
CR

Camila Rodrigues

Auburn University
NA
DW

Daniel Wells

Auburn University
NA
MT

Marlee Trandel Hayse

Auburn University
TR

Tanzeel Rehman

Auburn University
NA
Thursday September 26, 2024 1:15pm - 1:25pm HST
South Pacific 3

1:25pm HST

PH 2 - Modified Hydrocooling and Optimized Postharvest Handling Practices Can Improve the Shelf Life of Water Spinach During Su
Thursday September 26, 2024 1:25pm - 1:35pm HST
In an effort to improve the shelf life of water spinach (Ipomoea aquatica Forsk.) and amaranth (Amaranthus tricolor L.) during summer in humid subtropical regions like northern Taiwan, the study investigated the effectiveness of modified hydrocooling and optimized postharvest handling practices. Most farmers in Taiwan often compromised postharvest vegetable quality due to uneven cooling. The first trial implemented a modified hydrocooling system using a 10-minute 5°C shower with a multi-hole perforated pipe and smaller baskets for precooling to substitute the conventional method. The results showed that the modified hydrocooling improved cooling uniformity and reduced vegetable loss rates. Meanwhile, for some farmers needed to meet the specialized market requirement which extend vegetable shelf life, the second trial conducted optimized postharvest handling practices including storage vegetables at 11°C, consistently clean 10°C water for washing/hydrocooling, and wrapping vegetables in plastic film. These practices revealed significantly decreased the water spinach quality loss for longer duration but less effective for amaranth. Even with the addition of hypochlorous acid in washing/hydrocooling water, the shelf life of amaranth was difficult to extend due to severe pre-harvest leaf disease. In conclusion, the study showed that modified hydrocooling offers benefits for most conventional farmers by reducing vegetable loss rates. On the other hands, for farmers needed to meet the specialized market requirement, optimized postharvest handling practices significantly improves the quality of water spinach in longer shelf life. Also, the research emphasized the importance of disease management in amaranth cultivation for postharvest quality.
Speakers
WS

Wei Shiang Liao

Taoyuan District Agricultural Research and Extension Station, Ministry of Agriculture
Co-authors
AC

Ah Chiou Lee

Taoyuan District Agricultural Research and Extension Station, Ministry of Agriculture
NA
WF

Wing Fu Fung

Taoyuan District Agricultural Research and Extension Station, Ministry of Agriculture
NA
Thursday September 26, 2024 1:25pm - 1:35pm HST
South Pacific 3

1:35pm HST

PH 2 - Discovery of Biomarkers for Postharvest Pecan Color Stability Using Metabolomics and Machine Learning Algorithms
Thursday September 26, 2024 1:35pm - 1:45pm HST
Nut kernel color is a crucial quality indicator affecting consumers' first impression of the product. In the postharvest stage, nut kernels (e.g., pecans) gradually darken with increasing blue/red colors, which have a negative impact on consumers' preference. While growing evidence suggests that plant phenolics and their derivatives are linked to pecan kernel color, the compounds (biomarkers) responsible for kernel color stability during storage remain elusive. We hypothesized that: (1) the phenotype of pecan color exhibits notable variation among different cultivars during storage; and (2) this variation is attributed to changes in metabolites and their combination effects in the stage of phenolic biosynthetic pathways related to color formation. Here, pathway-based metabolomics with machine learning (ML) algorithms were employed to identify key metabolites of postharvest pecan color stability. Nine pecan cultivars with different color stabilities were selected, and the color of each cultivar was measured over a 6-month storage period (0, 1, 2, 3, and 6 months). Metabolites in phenylpropanoid, flavonoid, and anthocyanin biosynthetic pathways were analyzed in the outer layer of the kernel (testa) using liquid chromatography–mass spectrometry. Different ML models were compared to find relevant biomarkers of pecan color phenotypes. Lasso regression and support vector machine (linear kernel) in conjunction with recursive feature elimination were chosen as the most effective models to select biomarkers that predict the kernel color of pecans. Results revealed twenty marker compounds (flavonoid precursors, flavonoids, anthocyanidins, and anthocyanins) within phenylpropanoid, flavonoid, and anthocyanin biosynthetic pathways, and their roles in pecan color phenotypes during storage. Our findings provide a foundation for future research in the area and will help select genes/proteins for the breeding of pecans with stable and desirable kernel color. The collected information on biomarkers and their effects will also help set up strategies for the quality control of pecans after harvest.
Speakers
MJ

Min Jeong Kang

University of Georgia
Co-authors
JS

Joonhyuk Suh

University of Georgia
NA
LW

Lenny Wells

University of Georgia
NA
PC

Patrick Conner

University of Georgia
RP

Ronald Pegg

University of Georgia
NA
WK

William Kerr

University of Georgia
NA
Thursday September 26, 2024 1:35pm - 1:45pm HST
South Pacific 3

1:45pm HST

PH 2 - Evaluating the Effects of Controlled Atmosphere Storage on Two Georgia-grown Pecan Cultivars
Thursday September 26, 2024 1:45pm - 1:55pm HST
Pecans are an important commodity in Georgia with 180,000 planted acres in 2022. The crop undergoes quality deterioration through lipid oxidation, off-flavor development, and kernel browning in storage. Many new varieties have been introduced to the State with little known about their storage viability. Knowing the varieties performance after harvest can help growers plan for an uncertain market by reducing storage costs. The standard industry technique for storage is low temperature (0°C) cold rooms, which can quickly become expensive over long periods of time due to rising electricity costs. The addition of controlled atmosphere (CA) may possibly aid in reducing oxidation rate and kernel browning during storage. The controlled atmosphere would reduce the amount of oxygen (O2) substituted by carbon dioxide (CO2). Previous studies reported shelf-life increases after oxygen levels were reduced to 2-3% using nitrogen (N2), however carbon dioxide is less commonly seen. Half pecan kernels were hand shelled and sorted before being stored under varied conditions for up to five months. Three treatments were applied to two GA pecan varieties (‘Desirable’ and ‘Sumner’). Conditions included elevated storage temperature at 10 °C, low temperature storage (5 °C) plus CA (10% CO2 4% O2), high temperature storage plus CA, and a control of cold storage at (0 °C). Quality assessments were conducted every month for change of weight, firmness, and color values. Firmness values were conducted using MecMesin texture analyzer to penetrate the half kernel until the breakpoint was achieved. A significant difference was seen in ‘Desirable’ when comparing the days of storage against the atmospheric conditions in the chroma values with the CA treatments exhibiting higher chroma. The Sumner variety saw significant differences in the chroma after two months of storage and varied differences in lightness values throughout storage. A significant difference was seen between the two varieties in firmness values with Sumner exhibiting higher firmness.
Speakers
RC

Ramsey Corn

University of Georgia
Co-authors
AD

Angelos Deltsidis

University of Georgia
CE

Camille Esmel McAvoy

University of Georgia
NA
OG

Orestis Giannopoulos

University of Georgia
Thursday September 26, 2024 1:45pm - 1:55pm HST
South Pacific 3

2:00pm HST

Postharvest Interest Group Meeting
Thursday September 26, 2024 2:00pm - 3:00pm HST
Moderator
RB

Randolph Beaudry

Michigan State University
Dr. Randolph Beaudry, Professor, MSU Department of Horticulture (MSc, PhD, University of Georgia). His appointment is 50% MSU Extension and 50% AgBioResearch. His research program includes both regional and international projects and focuses on the physiology of plant responses to... Read More →
Thursday September 26, 2024 2:00pm - 3:00pm HST
Sea Pearl 4
 
Friday, September 27
 

10:00am HST

Workshop: Preparing for AI in Postharvest Research
Friday September 27, 2024 10:00am - 12:00pm HST

Explore cutting-edge techniques, tools, and strategies to integrate artificial intelligence (AI) into postharvest research. Learn how AI can enhance data analysis, optimize storage conditions, and improve quality assessment for fruits and vegetables. During this workshop, we’ll delve into organizing datasets, determining the number of variables, and understanding their types. Join us to stay ahead in the rapidly evolving field of postharvest science. Join us for this dynamic workshop, where experts share insights, foster collaboration, and propel postharvest science into the future! 🌱

The goal of this workshop is to empower researchers, practitioners, and industry professionals with the knowledge and tools needed to revolutionize postharvest practices. By integrating artificial intelligence (AI) and non-destructive technologies, we aim to achieve the following objectives: 1. Enhanced Quality Control 2. Efficient Resource Management 3. Scientific Advancements 4. Sustainable Practices 5. Industry Transformation

Coordinator(s)
  • Randolph Beaudry, Michigan State University, Horticulture, East Lansing, Michigan, United States
  • Angelos Deltsidis, University of Georgia, Horticulture, Tifton, GA, United States
Moderator(s)
  • Angelos Deltsidis, University of Georgia, Horticulture, Tifton, GA, United States
Speaker/Participant(s)
  • Carolina Torres, Washington State University, Wenatchee, Washington, United States
    Introduction (10 minutes) Welcome and Objectives (10 mins)
    Summary: Overview of AI applications, emphasizing data analysis, optimization, and quality assessment and discuss principles for designing experiments that maximize AI potential.
  • Luan Oliveira, University of Georgia, Tifton, Georgia, United States
    Remote Sensing and AI (20 mins)
    Summary: Explore how remote sensing, combined with AI algorithms, accelerates data processing for fruits and vegetables.
  • Pavlos Tsouvaltzis, Southwest Florida Research and Education Center, University of Florida, United States
    Non-Destructive Technologies (20 mins)
    Summary: Delve into advanced non-destructive methods for assessing vegetable crop physiology, quality, and safety.
  • Loren Honaas, USDA ARS TFRL, United States
    AI in Molecular Biology (20 mins)
    Summary: Discuss molecular indicators related to postharvest tree fruit disorders using AI techniques.

Moderator Speakers
CT

Carolina Torres

Washington State University
RB

Randolph Beaudry

Michigan State University
Dr. Randolph Beaudry, Professor, MSU Department of Horticulture (MSc, PhD, University of Georgia). His appointment is 50% MSU Extension and 50% AgBioResearch. His research program includes both regional and international projects and focuses on the physiology of plant responses to... Read More →
Friday September 27, 2024 10:00am - 12:00pm HST
Coral 2

12:14pm HST

Postharvest 3 (PH 3)
Friday September 27, 2024 12:14pm - 1:35pm HST
Evaluating the Potential of Gaseous Ozone to Suppress Decay Incidence in Fresh Peaches During Cold Storage - Orestis Giannopoulos
Evaluation of different reflectance models to predict fruit firmness using compression values in sweet cherries cv ‘Skeena’ - Rene Mogollon
Effects of Harvest Regimes on Shelf-life and Quality of Rabbiteye Blueberries - Amit Godara
Facilitating Postharvest Elderberry Fruit Detachment with Ethylene - Steven Sargent
Integrated Analyses of Transcriptomics and Lipidomics Reveal Potential Mechanisms Related to Physiological Disorders of Cold-Stored Hardy Kiwifruit (Actinidia arguta) - Hyowon Park
β-Cyclodextrin Nano-encapsulated Carvacrol Inclusion Complexes Improve Postharvest Quality of Limes - Chang Shu
Nondestructive Detection of Chilling Injury Based on VIS/NIR Spectroscopy in Sweet Potato - Jonghwan Lee
Development and Characterization of Bio-based Nanoparticle Coatings from Sweetpotato Peels - David Picha

Moderator
CS

Chang Shu

USDA-Agricultural Research Service-Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center
Friday September 27, 2024 12:14pm - 1:35pm HST
Lehua Suite

12:15pm HST

PH 3 - Evaluating the Potential of Gaseous Ozone to Suppress Decay Incidence in Fresh Peaches During Cold Storage
Friday September 27, 2024 12:15pm - 12:25pm HST
Sanitizing fruit and vegetables with gaseous ozone promotes food safety, marketability, and extended postharvest shelf-life. Ozone is a self-decomposing gas that disinfects and neutralizes microorganisms on packing equipment, water, and produce surfaces. The USDA permits ozone use in "organic" products. Ozone is produced on-site through a corona discharge mechanism using commercially gaseous ozone generators. This study studied gaseous ozone application on peaches, ranging from 0.5 - 5 ppm compared to control storage (CS). Gaseous ozone applications are known for postharvest spoilage vulnerability to assess microbial load reduction and defense against diseases like brown rot and gray mold. Investigated varieties (July Prince, Scarlet Prince, August Lady, and O’Henry), stored under controlled conditions of 35°F (1.7°C) at 90% relative humidity, revealed that 5 ppm ozone resulted in high decay (~40-42%). However, 0.5 ppm treatment maintained peach quality comparable to controls, with decay rates between 3.5 to 7%. Notably, peaches treated with 0.5 ppm also exhibited higher firmness than the other treatments and unaffected visual quality up to the 28th storage day. The findings suggest ozone’s potential to enhance postharvest practices, highlighting its efficiency, and could serve as an effective counteract treatment for decay incidence that will benefit the peach industry.
Speakers
avatar for Orestis Giannopoulos

Orestis Giannopoulos

University of Georgia
Co-authors
AD

Angelos Deltsidis

University of Georgia
CE

Camille Esmel McAvoy

University of Georgia
NA
DC

Dario Chavez

University of Georgia
NA
RC

Ramsey Corn

University of Georgia
Friday September 27, 2024 12:15pm - 12:25pm HST
Lehua Suite

12:25pm HST

PH 3 - Evaluation of different reflectance models to predict fruit firmness using compression values in sweet cherries cv ‘S
Friday September 27, 2024 12:25pm - 12:35pm HST
The compression test is the standard procedure to measure fruit firmness in sweet cherries. Nevertheless, this measurement is not always well correlated with perceived texture by buyers and consumers; therefore, the cherry industry needs a better way to grade fruit firmness. Hyperspectral information was correlated to fruit firmness as an alternative to compression values. ‘Skeena’ cherries, grown under commercial conditions in central Washington, were harvested in 2023 and stored for 30 days at 0-1oC. Compression measurements (at 20oC; FirmTech 2, BioWorks Inc) were carried out at harvest and 15 and 30 days into storage. Immediately after these measurements, hyperspectral images from the fruit (n=1030) were taken using a Vi-NIR camera (Headwall Photonics). The comparison between low (< 303 mm/g), medium (303-374 mm/g), and high firmness (>374 mm/g) groups did not yield any spectral differences. Despite this, iPLS wavelength selection showed bands > 800 nm suitable to model these compression groups. On the other hand, Neural Network, Random Forest, and PLS models were not able to predict compression values (regression) or firmness groups (classification). Furthermore, the regression models tested did not have coefficients of determination higher than 0.42 with root mean squared errors of 40 mm/g for compression values with the training dataset. Classification models achieved total accuracies of around 65-70 % and had problems distinguishing between low-medium and medium-high compression values. All models showed poor performance when tested with an independent data set. These results are in contrast to previous reports, which used a lower fruit number, reinforcing the challenge of tailoring a non-destructive technique to predict firmness through compression values in sweet cherries, a highly variable phenotypic characteristic.
Speakers
RM

Rene Mogollon

Washington State University
Co-authors
CT

Carolina Torres

Washington State University
Friday September 27, 2024 12:25pm - 12:35pm HST
Lehua Suite

12:35pm HST

PH 3 - Effects of Harvest Regimes on Shelf-life and Quality of Rabbiteye Blueberries
Friday September 27, 2024 12:35pm - 12:45pm HST
Blueberry producers in the United States are facing high input costs and labor shortages, which have led them to mechanize several areas of blueberry production, particularly harvesting. Even though the use of machine harvesters is advantageous to producers and new technologies have been developed in recent years, several parts of the process still require improvements. One of the issues caused by the adoption of mechanical harvesting is increased harvest intervals, due to the high capital expenses associated with the purchase of such machinery that in turn leads to limited availability of harvesters to perform the harvest in a timely fashion. This trend may compromise berry quality, particularly in the Southeastern United States. Firmness is a critical factor in determining consumer preference, shelf life, and market value of fresh blueberries. The postharvest quality of blueberries is influenced by various factors, including genotype, postharvest handling, and harvest conditions such as harvest intervals. This study aimed to investigate the impact of different harvest-regimes on fruit quality and storability of ‘Brightwell’ blueberries. The experiment was conducted at the Alma Blueberry Research Farm, with a randomized complete block design. Fruit were hand-harvested at different intervals: T1: every 2 days, T2: every 3 days, and T3: every 7 days. Each treatment was replicated four times, resulting in nine harvests across all treatments. Following the harvest, the berries were sorted for defects, packed in clamshells, and stored at 1°C (34°F) and 85% relative humidity. Fruit quality parameters: firmness, fruit size, color, total soluble solids (TSS), titratable acidity, and fruit rot evaluation were evaluated at four different intervals: 1, 7, 14, and 21 days after harvest. Preliminary results indicate that fruit from the second and third harvests, T1 and T2 exhibited higher firmness readings at harvest compared to T3. Throughout the storage period, the firmness of the fruit was consistently higher in T2 compared to other treatments. A bigger fruit size was obtained at the first harvest of all treatments which declined after 14 days of storage in all three treatments. At the second harvest, T2 had the highest TSS but after 21 days of storage, TSS declined and T1 had the highest TSS after 21 days of storage in all three harvests. Additionally, T3 had a higher fruit rot incidence, and wet, sunken berries in the second and third harvests. So, a harvest interval of less than 7 days is recommended to maintain the fruit quality.
Speakers
AG

Amit Godara

The University of Georgia
Co-authors
AD

Angelos Deltsidis

The University of Georgia
ZR

Zilfina Rubio Ames

The University of Georgia
Friday September 27, 2024 12:35pm - 12:45pm HST
Lehua Suite

12:45pm HST

PH 3 - Facilitating Postharvest Elderberry Fruit Detachment with Ethylene
Friday September 27, 2024 12:45pm - 12:55pm HST
Interest in producing elderberry (Sambucus nigra ssp. canadensis) fruit in North America has increased in recent years, including the subtropical climate of Florida. The fruit contain high amounts of antioxidants in both the juice and solid fractions and are processed into a wide variety of products. Fruit grow in clusters (cymes) of 500 or more fruit (1-2 mm diameter) each, and typically ripen at different rates, depending upon when the flowers were fertilized, the variety/selection and weather conditions. This non-uniform ripening from green to deep purple challenges growers, since unripe fruit must be removed. Delaying cyme harvest until all fruit ripen is not feasible since overripe fruit abscise before unripe fruit ripen. Fruit are typically destemmed from the cymes the day of harvest, either manually or with mechanized systems. Destemming can cause significant loss of salable product as the fruit are often crushed, releasing juice. In a series of tests, we investigated the potential to promote uniform ripening and detachment of elderberry fruit by postharvest exposure to gaseous ethylene, commercially used to initiate ripening in climacteric fruit crops. At a commercial farm in Chiefland, Florida, small segments (approximately 30 fruit each) were cut from cymes with 75% ripe fruit from three elderberry genotypes (DNS11, DNS23 and DNS36). The samples were returned to the UF Postharvest Laboratory that day, divided into two treatment groups ( /- 100 ppm ethylene) and sealed in glass jars (n=3; 3 segments/jar) at 22 °C/95% relative humidity. Jars were opened after 24, 48 and 72 hr to avoid CO2 accumulation; at each timepoint, one jar was removed and fruit examined; remaining jars were resealed and ethylene concentration was reestablished. Exposure to ethylene significantly increased ripe fruit detachment after 24 hr, however partially ripe fruit ripened after 48 hr, irrespective of treatment. Fruit juice content was 46%, pH=5.0. Ethylene did not affect the following parameters, although there were differences in germplasm: soluble solids content (7-14%), total titratable acidity (0.44-0.70%), and total anthocyanin content (8-20 mg · g-1). Attached or detached fruit lost 6-10% weight after 24 hr, whereas stems lost about 50% weight; after 48 hr, shrivel symptoms appeared and weight loss roughly doubled for these same groups. Consideration must be given as to exposure to ethylene for 24 hr for easy fruit removal versus holding for 48 hr for ripening with accompanied higher weight loss and shriveling.
Speakers
SS

Steven Sargent

University of Florida
Professor and Extension Postharvest Specialist
Co-authors
AB

Adrian Berry

University of Florida
NA
AS

Ali Sarkhosh

University of Florida
NA
DJ

David Jarnagin

Hydemoer Co.
NA
KA

Kevin Athearn

University of Florida
NA
Friday September 27, 2024 12:45pm - 12:55pm HST
Lehua Suite

12:55pm HST

PH 3 - Integrated Analyses of Transcriptomics and Lipidomics Reveal Potential Mechanisms Related to Physiological Disorders of
Friday September 27, 2024 12:55pm - 1:05pm HST
Hardy kiwifruit (Actinidia arguta) is highly sensitive to low temperatures, which can cause chilling injury (CI) as a physiological disorder. Peel surface pitting with black/dark brown spots was developed as CI symptoms in cold-stored hardy kiwifruit. In this study, we performed transcriptomic and lipidomic analyses to elucidate the potential mechanism of CI symptoms in cold-stored hardy kiwifruit. The peel tissues of hardy kiwifruit were collected after harvest (HV) and after cold storage; pitted peel tissues from CI-damaged fruit (PT) and healthy peel tissues from CI-unaffected fruit (HT) were also collected. Transcriptomic analysis identified 10,612 differential expressed genes (DEGs) between HV and HT tissues, and 3,206 DEGs were detected between HT and PT tissues. Among the 3,206 DEGs, 1,322 transcripts were up-regulated in HT tissues, while 1,874 transcripts were up-regulated in PT tissues. The KEGG pathway enriched by up-regulated DEGs in HT indicated protein modification, L-valine degradation, and polyol metabolism, while up-regulated DEGs in PT enriched alkaloid biosynthesis, protein modification, and lipid metabolism. In lipidomics analysis, a total of 101 lipids clustered into 14 subgroups were detected in peel tissues of cold-stored hardy kiwifruit. Based on the results of VIP scores (> 1.5), the levels of triacylglycerol (TAG) 54:8, TAG 54:9, phosphatidylcholine (PC) 36:4, PC 34:2, PC 36:5, PC 34:3, and TAG 54:7 were higher in PT tissues, but the levels of phosphatidylethanolamine (PE) 36:2, phosphatidylserine (PS) 38:3, PS 36:0, PC 36:1, PE 34:1, and PE 36:1, were higher in HT tissues, compared with the other tissues. Therefore, the results indicated that the CI symptoms in cold-stored hardy kiwifruit would be derived from integrated transcriptomic and lipidomic results.
Speakers
HP

Hyowon Park

Chung-Ang University
Co-authors
HJ

Hyun Ji Eo

National Institute of Forest Science
NA
JE

Jane E. Stewart

Colorado State University
NA
avatar for Jinwook Lee

Jinwook Lee

Professor, Chung-Ang University
NA
JR

Jorge R. Ibarra Caballero

Colorado State University
NA
UL

Uk Lee

National Institute of Forest Science
NA
Friday September 27, 2024 12:55pm - 1:05pm HST
Lehua Suite

1:05pm HST

PH 3 - β-Cyclodextrin Nano-encapsulated Carvacrol Inclusion Complexes Improve Postharvest Quality of Limes
Friday September 27, 2024 1:05pm - 1:15pm HST
The ripening and senescence of fresh fruit and vegetables are inevitable during storage, transportation, and sale, which will reduce their marketability and cause substantial economic losses. The infection of pathogenic microorganisms can also lead to food safety problems. Plant essential oils are considered a promising strategy to preserve fresh produce. However, their application is limited by several aspects, including high volatility, low solubility, off flavor, etc. Encapsulation technology has emerged as a promising solution to alleviate these limitations. Nanoencapsulation is a delivery system for bioactive compounds that is usually employed to support the stability of the agents, maximize their retention, and control the release of the compounds at the target locations. A nanoemulsion composed of carvacrol and β-cyclodextrin was prepared by an ultrasound-mediated method, and the encapsulation process was conducted using a Buchi® Nano Spray Dryer B-90. The inclusion complex powder was collected and filled to air-permeable sachets. The sachets were then fixed inside commercial clamshells containing limes to simulate shelf-life. Fruit quality and total microbial population were analyzed. The results showed that the dimensions of spray-dried powders were primarily at nanoscale. The encapsulation process did not affect the antimicrobial and antioxidant activities of carvacrol. The nanocapsules prolonged the release of carvacrol. It effectively delayed the rapid color deterioration of limes without causing phytotoxicity, and maintained lime quality with higher firmness and lower weight loss. Free carvacrol and carvacrol/β-cyclodextrin treatments significantly reduced the growth of P. digitatum and total bacteria on fruit peel and inside of the wounds. However, the GC-MS results showed carvacrol residue detected from free carvacrol-treated fruit, but not from the carvacrol/β-cyclodextrin-treated fruit. The results indicate that nano-encapsulated carvacrol/β-cyclodextrin inclusion complexes can be applied as an effective strategy to preserve postharvest produce. Keywords: Nanoencapsulation, nano spray drying, essential oil, postharvest quality, lime
Speakers
CS

Chang Shu

USDA-Agricultural Research Service-Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center
Co-authors
XS

Xiuxiu Sun

USDA-Agricultural Research Service-Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center
NA
Friday September 27, 2024 1:05pm - 1:15pm HST
Lehua Suite

1:15pm HST

PH 3 - Nondestructive Detection of Chilling Injury Based on VIS/NIR Spectroscopy in Sweet Potato
Friday September 27, 2024 1:15pm - 1:25pm HST
Sweet potato (Ipomoea batatas L.) is one of the seven major food crops grown worldwide. The optimal storage temperature for sweet potatoes is 12-15℃, and chilling injury occur when it falls below 10℃. The initial symptom of chilling injury in sweet potatoes is difficult to discern in visual judgment and requires checking through a cut-off sections. Therefore, this study intended to develop a non-destructive chilling injury prediction model using VIS/NIR spectroscopy sensors. USB4000 Fiber Optical Spectrometer was used for collecting spectrum data in the wavelength of the visible to near-infrared region (400 nm to 1100 nm), and the average value was used by measuring six areas divided into upper, middle, and lower parts to reduce errors. Firmness, total soluble solids, internal chromaticity, chilling index, and Malondialdehyde (MDA) were measured as reference data, and Partial Least Squares Regression(PLSR) method was used for prediction. To increase the accuracy of the model, the near-infrared spectrum data were preprocessed using the Savitzky–Golay 1st and 2nd derivatives (S–G), standard normal variate (SNV), and multiplicative scattering correction (MSC) methods. Excellent performance models for each preprocessing methods were selected, and as a result of verification through the prediction model, the 'Hogammi' variety showed excellent performance in the preprocessing of Savitzky-Golay 2st derivative in TSS and Malondialdehyde (MDA), and CI index showed the best performance in the processing of SNV. As indicators of chilling injury such as the CI index involve subjective opinions of experimenters, the findings of this study could be used as a fundamental step to develop more objective and accurate chilling injury prediction models by including various internal indicators.
Speakers
JL

jonghwan lee

kangwon national university
Co-authors
CJ

CheonSoon Jeong

kangwon national university
NA
HC

HanRyul Choi

National Institute of Horticultural and Herbal Science
NA
MB

MinWoo Baek

kangwon national university
NA
SJ

SeMin Jang

kangwon national university
NA
ST

Shimeles Tilahun

kangwon national university, jimma university
NA
ST

SuHo Tae

kangwon national university
NA
Friday September 27, 2024 1:15pm - 1:25pm HST
Lehua Suite

1:25pm HST

PH 3 - Development and Characterization of Bio-based Nanoparticle Coatings from Sweetpotato Peels
Friday September 27, 2024 1:25pm - 1:35pm HST
Bio-based nanoparticle coatings were developed using nanomaterials extracted from sweetpotato peels and combined with other biopolymers to reduce postharvest deterioration of sweetpotato roots and other fresh produce items. The sustainable coating materials applied as thin films to the surface of washed sweetpotato roots reduced postharvest deterioration, weight loss, and respiration rate, while maintaining root market quality. Chemical and physical characterization was made of the cellulose nanomaterials (CNMs), pectin, and phenolic compounds from ‘Beauregard’ sweet potato peel tissue. The peel contained 40.2% cellulose, 19.1 % lignin, 10.8% hemicellulose and 29.8% pectin. The extracted CNMs from the peel tissue showed a high degree of crystallinity, particularly for cellulose nanocrystals. The CNMs and pectin fractions formed stable water dispersions with strong shear thinning behavior suitable for coating application. The thin films formed by the CNMs and pectin fractions exhibited an entangled fiber network structure with significantly reduced water and gas permeability. Our results demonstrated the feasibility of using sweetpotato peel tissue as an integral component in a bio-based nanoparticle film coating.
Speakers
DP

David Picha

Louisiana State University Agricultural Center
Co-authors
QW

Qinglin Wu

LSU Agricultural Center
NA
RA

Ragab Abouzeid

School of Plant, Environmental and Soil Sciences
NA
Friday September 27, 2024 1:25pm - 1:35pm HST
Lehua Suite
 


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